FastQCFastQC Report
Thu 26 May 2016
SRR938127_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938127_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147556
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118650.5524885032101607No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97100.45214187662626726No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59220.2757553237261333No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34260.1595301822164358No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25470.11859993406458318No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA129300.031.4176121
GGTATCA89150.031.2337131
TATCAAC154800.026.0037232
ATCAACG156450.025.6079673
TCAACGC157950.025.3948674
CAACGCA159750.025.0194785
AACGCAG162950.024.66886
GTAAGGT9750.023.8844154
TAAGGTG10800.023.7625565
GAGTACT92350.023.50496512-13
GGACCGA6400.023.0151486
GTACTTT96500.022.32367314-15
ACGCAGA181550.022.0331737
GTACATG87300.022.0101471
CGCAGAG182550.021.860438
AGAGTAC138500.021.77883110-11
TACATGG85450.021.7464222
AGTACTT95000.021.47434212-13
GCAGAGT187100.021.021679
ACATGGG89500.019.8065553
Produced by FastQC (version 0.11.5)