FastQCFastQC Report
Thu 26 May 2016
SRR938127_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938127_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147556
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT112140.5221749747154439No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97760.45521513757964865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58750.27356678941084656No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT33570.15631722758335523No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25830.12027625822097306No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA89150.034.3011551
GTATCAA126750.028.9284761
TATCAAC153500.023.7013032
ATCAACG153200.023.681763
TCAACGC155450.023.338454
CAACGCA157300.023.0331975
AACGCAG161100.022.5537326
GAGTACT96350.022.52770812-13
GTACGCT1302.921902E-421.9460413
TAAGGTG11950.021.8838045
GTACTTT99300.021.73887814-15
TACATGG79600.021.0902042
GTACATG82350.020.963421
TACCTGG15450.020.9314632
TAAGGTA6850.020.8242234
GTAAGGT11500.020.6733254
AGAGTAC138350.020.66663710-11
ACGCAGA175750.020.5385977
CGCAGAG176100.020.4438368
AGTACTT100200.020.003112-13
Produced by FastQC (version 0.11.5)