##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938127_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2147556 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.44965626041882 33.0 31.0 34.0 31.0 34.0 2 32.67554000920116 34.0 31.0 34.0 31.0 34.0 3 32.740526440288406 34.0 31.0 34.0 31.0 34.0 4 36.210577512297704 37.0 35.0 37.0 35.0 37.0 5 36.13756614495734 37.0 35.0 37.0 35.0 37.0 6 36.195228902063555 37.0 37.0 37.0 35.0 37.0 7 36.16455729210321 37.0 37.0 37.0 35.0 37.0 8 36.15665668322502 37.0 36.0 37.0 35.0 37.0 9 37.83610858110335 39.0 38.0 39.0 35.0 39.0 10-11 37.8113867577842 39.0 38.0 39.0 35.0 39.0 12-13 37.72459344482752 39.0 38.0 39.0 35.0 39.0 14-15 38.93977153564331 40.0 38.0 41.0 35.5 41.0 16-17 38.848238648957235 40.0 38.0 41.0 35.0 41.0 18-19 39.11136566403856 40.0 38.5 41.0 36.0 41.0 20-21 39.08621009184394 40.0 39.0 41.0 36.0 41.0 22-23 38.97832210196149 40.0 38.5 41.0 35.0 41.0 24-25 38.865038210877856 40.0 38.0 41.0 35.0 41.0 26-27 38.69622375388581 40.0 38.0 41.0 34.5 41.0 28-29 38.56658219855501 40.0 38.0 41.0 34.0 41.0 30-31 38.316673465092414 40.0 38.0 41.0 34.0 41.0 32-33 38.070832145937054 40.0 37.0 41.0 33.5 41.0 34-35 37.973269614389565 40.0 37.0 41.0 33.0 41.0 36-37 37.911139919052175 40.0 37.0 41.0 33.0 41.0 38-39 37.716820422843455 40.0 37.0 41.0 33.0 41.0 40-41 37.49666853856198 40.0 37.0 41.0 33.0 41.0 42-43 37.221162335231305 40.0 36.5 41.0 32.0 41.0 44-45 36.92994292116248 40.0 36.0 41.0 31.0 41.0 46-47 36.52404524026382 39.0 35.5 41.0 30.5 41.0 48-49 36.068944185855926 39.0 35.0 40.5 29.5 41.0 50-51 35.663462559299965 39.0 35.0 40.0 28.0 41.0 52-53 35.10726635300779 38.0 34.0 40.0 26.5 41.0 54-55 34.718932824103305 38.0 34.0 40.0 26.0 41.0 56-57 34.31428563446076 38.0 34.0 40.0 25.0 41.0 58-59 33.78813893560866 37.0 33.0 40.0 23.0 41.0 60-61 33.26802001903559 36.5 32.5 39.0 22.0 41.0 62-63 32.692602660885214 36.0 31.5 39.0 19.5 40.0 64-65 32.096620763323514 35.0 31.0 39.0 17.5 40.0 66-67 31.50418708522618 35.0 30.5 38.0 12.0 40.0 68-69 30.857673560084116 35.0 29.5 37.5 7.5 40.0 70-71 30.209280456481693 34.0 29.0 37.0 4.5 39.0 72-73 29.516630299745387 34.0 28.0 36.0 2.0 39.0 74-75 28.856700593605012 34.0 27.5 36.0 2.0 38.5 76-77 27.579410734807382 31.5 26.0 34.5 2.0 36.5 78-79 28.227375677281525 33.0 27.0 35.0 2.0 37.0 80-81 27.946102918852873 33.0 26.5 35.0 2.0 37.0 82-83 27.384038646722132 33.0 26.0 35.0 2.0 36.0 84-85 26.716864193529762 33.0 25.0 35.0 2.0 36.0 86-87 25.992559681796422 32.0 23.5 35.0 2.0 35.0 88-89 25.169942949101213 31.5 19.5 34.0 2.0 35.0 90-91 24.505665975648597 31.0 16.5 34.0 2.0 35.0 92-93 23.55524209845983 31.0 4.5 34.0 2.0 35.0 94-95 22.631248963938543 30.0 2.0 34.0 2.0 35.0 96-97 21.561228205457738 29.5 2.0 34.0 2.0 35.0 98-99 20.137451363317183 29.0 2.0 34.0 2.0 35.0 100-101 16.360037177144623 13.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05393950772173639 1101 2 0.10543957230770928 1101 3 0.14569623177030877 1101 4 0.007431456636005862 1101 5 -0.035829507924418635 1101 6 0.039131457655479096 1101 7 0.10138332319812804 1101 8 0.1265386776016797 1101 9 -0.05886251960159683 1101 10-11 0.08799873904958133 1101 12-13 0.20101354655210457 1101 14-15 0.3074056656437065 1101 16-17 0.2591050665511361 1101 18-19 0.2698515344063708 1101 20-21 0.21902532667785124 1101 22-23 0.13409920672663844 1101 24-25 0.20555070401742626 1101 26-27 0.32269235844883326 1101 28-29 0.2362421370043961 1101 30-31 0.27188253931585393 1101 32-33 0.2611526360697738 1101 34-35 0.27534402371557576 1101 36-37 0.2995093817692336 1101 38-39 0.3195413751005489 1101 40-41 0.3248440864708826 1101 42-43 0.4170056342460313 1101 44-45 0.445789352331488 1101 46-47 0.4232183552175144 1101 48-49 0.4728476066178544 1101 50-51 0.43752919398802703 1101 52-53 0.6986903305040215 1101 54-55 0.8653641866274029 1101 56-57 0.7455360728024658 1101 58-59 1.0001071510103756 1101 60-61 0.9487581770549411 1101 62-63 0.9328442929179204 1101 64-65 1.180605469495795 1101 66-67 1.406945514281336 1101 68-69 1.5833621017899588 1101 70-71 1.81706266899522 1101 72-73 1.996091931352499 1101 74-75 2.014934920065759 1101 76-77 2.104358487686067 1101 78-79 2.116861320571477 1101 80-81 2.2835675256465144 1101 82-83 2.3673154411846085 1101 84-85 2.5376796582022862 1101 86-87 2.829304756333574 1101 88-89 3.124773606753969 1101 90-91 3.36065426751912 1101 92-93 3.419998775414271 1101 94-95 3.6421424548748504 1101 96-97 3.729320568779851 1101 98-99 4.101662479550001 1101 100-101 3.951597150828597 1102 1 0.13814195939901452 1102 2 0.11125640570902817 1102 3 0.10617053423323597 1102 4 0.03012989666839161 1102 5 -0.009321016907236412 1102 6 -0.005976249611855167 1102 7 0.037573608941613656 1102 8 0.11066636969260912 1102 9 0.11358932603860694 1102 10-11 0.1556183566030498 1102 12-13 0.22484888760320842 1102 14-15 0.3318075372622076 1102 16-17 0.30916297495453904 1102 18-19 0.2418952594600512 1102 20-21 0.2312941988950854 1102 22-23 0.2669842976093051 1102 24-25 0.22420722315082742 1102 26-27 0.31692514816290895 1102 28-29 0.3488195586641183 1102 30-31 0.40898603807040246 1102 32-33 0.2957988749477707 1102 34-35 0.44730022895323174 1102 36-37 0.5432915020290849 1102 38-39 0.7051590603062721 1102 40-41 0.7265810136598887 1102 42-43 0.7869814045784338 1102 44-45 0.5855364634834288 1102 46-47 0.7195317121946871 1102 48-49 0.8565716382951152 1102 50-51 0.9691562502827935 1102 52-53 0.9354998122998737 1102 54-55 1.0169345957729448 1102 56-57 1.1723326732646058 1102 58-59 1.1993473561310637 1102 60-61 1.055708839567842 1102 62-63 1.2892248065112568 1102 64-65 1.305849929798633 1102 66-67 1.4603326020513343 1102 68-69 1.738663548924066 1102 70-71 1.7723388024329623 1102 72-73 1.9352972644195994 1102 74-75 2.0847763535357835 1102 76-77 2.056981852080117 1102 78-79 2.097654073469922 1102 80-81 2.154163584921328 1102 82-83 2.1442536299822486 1102 84-85 2.427447224120538 1102 86-87 2.4664593550819482 1102 88-89 2.551688505178703 1102 90-91 2.7562518110567567 1102 92-93 3.0543896915485433 1102 94-95 3.120518886966945 1102 96-97 3.415024001547472 1102 98-99 3.8569886200915278 1102 100-101 3.5630097584671887 1103 1 0.0954187466391474 1103 2 0.09102040956120305 1103 3 0.03923736687404045 1103 4 0.022490452689396534 1103 5 -0.08348577763823073 1103 6 -2.04818718643196E-5 1103 7 0.028415352278926775 1103 8 0.07160002589809977 1103 9 0.17861019104379494 1103 10-11 0.15938278898558877 1103 12-13 0.19495702833005168 1103 14-15 0.2807989255828005 1103 16-17 0.30872941827034595 1103 18-19 0.18951460387178543 1103 20-21 0.12725768420255434 1103 22-23 0.20253715203219969 1103 24-25 0.2852930478434246 1103 26-27 0.28635832485671386 1103 28-29 0.20311220066578528 1103 30-31 0.3580401836851337 1103 32-33 0.3935998708500179 1103 34-35 0.46196245828188864 1103 36-37 0.35778409997138994 1103 38-39 0.40564420147571667 1103 40-41 0.5556578610436347 1103 42-43 0.46086089965938726 1103 44-45 0.47577711544698076 1103 46-47 0.674735929025303 1103 48-49 0.9373633969555542 1103 50-51 1.0215522720735066 1103 52-53 0.8862975192603599 1103 54-55 1.0730172136683365 1103 56-57 1.1330276506992618 1103 58-59 1.348415029748331 1103 60-61 1.322248833197328 1103 62-63 1.1400571807733542 1103 64-65 1.2620654438038557 1103 66-67 1.41680158659236 1103 68-69 1.3940047978143184 1103 70-71 1.57571876381353 1103 72-73 1.8149304975806153 1103 74-75 1.8963627286830906 1103 76-77 1.7265875323476472 1103 78-79 1.6050380363819414 1103 80-81 1.9424767693815284 1103 82-83 2.061835208002204 1103 84-85 2.23639836735164 1103 86-87 2.3346490756956655 1103 88-89 2.698208835813425 1103 90-91 2.8182843087080265 1103 92-93 2.886288319418469 1103 94-95 3.176246933330944 1103 96-97 3.633610816426664 1103 98-99 3.9016594631101142 1103 100-101 3.6407781090877407 1104 1 0.1767767565257543 1104 2 0.1978372978866645 1104 3 0.15849341561803243 1104 4 0.10045838687510411 1104 5 0.029475503928402702 1104 6 0.06020142437474618 1104 7 0.12504868922884782 1104 8 0.09620234394088101 1104 9 0.09689052232328521 1104 10-11 0.2248963350123816 1104 12-13 0.22726172780107845 1104 14-15 0.2687052445240141 1104 16-17 0.35226552800144617 1104 18-19 0.29277010441713003 1104 20-21 0.3004837000355991 1104 22-23 0.32070536907318825 1104 24-25 0.3557327678814133 1104 26-27 0.3321662231049558 1104 28-29 0.38416241985006394 1104 30-31 0.38554053106796005 1104 32-33 0.4100810446042473 1104 34-35 0.45673268119697497 1104 36-37 0.5786777018459262 1104 38-39 0.6617176905507662 1104 40-41 0.6914883534516534 1104 42-43 0.8216365246153643 1104 44-45 0.814620035594622 1104 46-47 0.8413435881980149 1104 48-49 0.9126183188467607 1104 50-51 0.9251939861840981 1104 52-53 1.0916188531342783 1104 54-55 1.166068224695124 1104 56-57 1.2639386312325058 1104 58-59 1.5058022921242014 1104 60-61 1.5907538688207197 1104 62-63 1.7742735327016632 1104 64-65 1.8167320800150364 1104 66-67 1.9447492488486908 1104 68-69 2.1985862538527883 1104 70-71 2.029763346830414 1104 72-73 2.3142366836597894 1104 74-75 2.1642405717498043 1104 76-77 2.2678071220742524 1104 78-79 2.296307972825158 1104 80-81 2.509212341573388 1104 82-83 2.7096708702280807 1104 84-85 2.930692913726773 1104 86-87 3.1010536348811364 1104 88-89 3.172353084391059 1104 90-91 3.439453342336588 1104 92-93 3.5641540373969107 1104 94-95 3.8525651321938135 1104 96-97 4.085655806785603 1104 98-99 4.216299793399802 1104 100-101 4.108001349665102 1105 1 0.16836692925367913 1105 2 0.23077671885046414 1105 3 0.18675168423646937 1105 4 0.16377782652796924 1105 5 -0.013776922134653091 1105 6 -0.0037587543518569078 1105 7 0.13435159101020133 1105 8 0.19482742044750978 1105 9 0.2926111773495208 1105 10-11 0.18209704652722536 1105 12-13 0.2208809922153563 1105 14-15 0.35701579277986895 1105 16-17 0.43721930416061383 1105 18-19 0.3527426724582199 1105 20-21 0.26632981959131286 1105 22-23 0.3714716285252919 1105 24-25 0.35426546524821845 1105 26-27 0.40779056926043467 1105 28-29 0.38676688202399134 1105 30-31 0.395520854488872 1105 32-33 0.39177365181063095 1105 34-35 0.47776638828630524 1105 36-37 0.4497898893174508 1105 38-39 0.38981407354823006 1105 40-41 0.5045927114166275 1105 42-43 0.5485774702538393 1105 44-45 0.6218205313296679 1105 46-47 0.5694562181450635 1105 48-49 0.6954848189314262 1105 50-51 0.6754784981955453 1105 52-53 0.7983734716470181 1105 54-55 0.8969894191200822 1105 56-57 0.9160127447392767 1105 58-59 1.1463225397581738 1105 60-61 1.0957264508240314 1105 62-63 1.3180491613126435 1105 64-65 1.4018725691650005 1105 66-67 1.585113500380917 1105 68-69 1.8578165139994525 1105 70-71 1.8555641881685432 1105 72-73 2.042499217102492 1105 74-75 2.0414166792903927 1105 76-77 2.1874717951894347 1105 78-79 2.350634218172882 1105 80-81 2.4334097866898006 1105 82-83 2.3610057475293402 1105 84-85 2.4915033420344415 1105 86-87 2.6786791482783947 1105 88-89 2.617392192575686 1105 90-91 2.8740827253002905 1105 92-93 2.8982921024512223 1105 94-95 3.161322542643294 1105 96-97 3.561056655841032 1105 98-99 3.6733926150151532 1105 100-101 3.5635719082631496 1106 1 0.15573724809707556 1106 2 0.023714883087507133 1106 3 0.030188626312195765 1106 4 0.07822653456491224 1106 5 -0.061808546685156784 1106 6 -0.007773345787569497 1106 7 -0.0184617111420593 1106 8 0.019932543623731647 1106 9 0.12620570145161025 1106 10-11 0.07137386972971882 1106 12-13 0.08264759028590163 1106 14-15 0.01903441731187172 1106 16-17 0.047768847775707 1106 18-19 0.015035264918182634 1106 20-21 -0.09893251311100926 1106 22-23 -0.00869576107223935 1106 24-25 -0.0503320530349356 1106 26-27 -0.08668849634551634 1106 28-29 0.009229464373589735 1106 30-31 0.1307184754859705 1106 32-33 0.16202859338697095 1106 34-35 0.1024757479679792 1106 36-37 0.0313381253364895 1106 38-39 0.06612529246278598 1106 40-41 0.13300604538761718 1106 42-43 0.08784007836602825 1106 44-45 0.09091346437502779 1106 46-47 0.17209938381081713 1106 48-49 0.2902206453568823 1106 50-51 0.4583724935155047 1106 52-53 0.30648075941019215 1106 54-55 0.5643692835828702 1106 56-57 0.5710890460088294 1106 58-59 0.8623539412891787 1106 60-61 0.6229223338070398 1106 62-63 0.6704388465643518 1106 64-65 0.7039497267044865 1106 66-67 0.8781942961664306 1106 68-69 0.9902762821697166 1106 70-71 1.0303952593038304 1106 72-73 1.2613753189522292 1106 74-75 1.53291682432517 1106 76-77 1.4243600866893864 1106 78-79 1.6297526230258654 1106 80-81 1.4210116564968729 1106 82-83 1.9263885540231485 1106 84-85 1.8723813659854862 1106 86-87 2.0915693633215895 1106 88-89 2.2624056999155044 1106 90-91 2.3229984010962568 1106 92-93 2.333673642444168 1106 94-95 2.674570454058408 1106 96-97 3.3456244990717785 1106 98-99 3.6134776315890065 1106 100-101 3.669361018807045 1107 1 0.2844175899021977 1107 2 0.2462307669574102 1107 3 0.22974543694798655 1107 4 0.1812651046250977 1107 5 0.15643489140826006 1107 6 0.17610600185774672 1107 7 0.18417310569516587 1107 8 0.26867192372166215 1107 9 0.2572872240225905 1107 10-11 0.2988400893745933 1107 12-13 0.34033887981932764 1107 14-15 0.4481506148256713 1107 16-17 0.44427511793291785 1107 18-19 0.3661947040307467 1107 20-21 0.31057029579734774 1107 22-23 0.4182374910704283 1107 24-25 0.5253573046716795 1107 26-27 0.4815324794706939 1107 28-29 0.4901310876427516 1107 30-31 0.5421757035960155 1107 32-33 0.5234831837467198 1107 34-35 0.4990154927141859 1107 36-37 0.5913911831514795 1107 38-39 0.681901002780549 1107 40-41 0.7243322944096988 1107 42-43 0.8072928599151155 1107 44-45 0.7466283810540588 1107 46-47 0.8944937892252369 1107 48-49 0.9227757595525432 1107 50-51 0.8403895894494156 1107 52-53 0.9388790280050827 1107 54-55 1.092691979567114 1107 56-57 1.1696732483212031 1107 58-59 1.248063887642303 1107 60-61 1.242556966356588 1107 62-63 1.4394483322929403 1107 64-65 1.5638489906656972 1107 66-67 1.6932470736465213 1107 68-69 2.0127644637472457 1107 70-71 2.0706176393967866 1107 72-73 2.1783695752805237 1107 74-75 2.1512568301021986 1107 76-77 2.089400810951748 1107 78-79 2.0968760818049255 1107 80-81 2.3991144256142363 1107 82-83 2.748037718092874 1107 84-85 2.5498885949590075 1107 86-87 2.7072547040775277 1107 88-89 2.6795350374474722 1107 90-91 2.9778726679376994 1107 92-93 3.486523347217222 1107 94-95 3.6790148858858096 1107 96-97 3.8121444689706685 1107 98-99 3.8620705751456477 1107 100-101 3.8024028748180534 1108 1 0.16660272342608096 1108 2 0.19381245281506665 1108 3 0.14327476197905753 1108 4 0.18075012189903106 1108 5 0.04162549394767723 1108 6 0.09608283364841697 1108 7 0.1270975773808587 1108 8 0.10000990945849253 1108 9 0.1296530488933456 1108 10-11 0.20767118923193095 1108 12-13 0.17799087609297715 1108 14-15 0.33491166211916834 1108 16-17 0.3459102903150253 1108 18-19 0.21189062946143622 1108 20-21 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56-57 0.5429279029915222 2316 58-59 0.5883828557948689 2316 60-61 0.7344184479691691 2316 62-63 0.8017263666061112 2316 64-65 1.0696253368015078 2316 66-67 1.3260411740783233 2316 68-69 1.4878107483053142 2316 70-71 1.6015959759489142 2316 72-73 1.7813063214747054 2316 74-75 2.1253838037495427 2316 76-77 2.1803276135713823 2316 78-79 2.1771646022913558 2316 80-81 2.0438838868122318 2316 82-83 2.2982538976320193 2316 84-85 2.711327617053353 2316 86-87 2.8036189598046377 2316 88-89 2.974768194926302 2316 90-91 3.172905651984671 2316 92-93 3.414334878678975 2316 94-95 3.7791169514041414 2316 96-97 4.084809662446691 2316 98-99 4.32959417421365 2316 100-101 4.555541079740983 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3.0 3 4.0 4 17.0 5 29.0 6 94.0 7 219.0 8 582.0 9 1049.0 10 1951.0 11 3106.0 12 4717.0 13 7259.0 14 9646.0 15 13450.0 16 18167.0 17 25330.0 18 26345.0 19 21356.0 20 20649.0 21 21785.0 22 23880.0 23 26433.0 24 29406.0 25 33033.0 26 37443.0 27 43047.0 28 51683.0 29 61542.0 30 72650.0 31 86960.0 32 105383.0 33 131002.0 34 169902.0 35 230828.0 36 312094.0 37 369346.0 38 180178.0 39 6988.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.40627205996025 18.712434041300902 15.283885495884626 22.59740840285422 2 16.021277626264883 19.974786143276553 39.34931111309817 24.654625117360396 3 19.655441531602765 23.596485591165923 29.75471035649792 26.993362520733395 4 13.30460871358996 16.84352967307856 36.01984429474198 33.8320173185895 5 13.880524721185344 36.93308774614079 33.90333990190498 15.283047630768888 6 32.61608322770929 35.75612068427616 16.62244581509941 15.005350272915141 7 29.01228186831915 32.78470968859485 19.737087181894207 18.46592126119179 8 26.982654652976645 34.198924355661106 18.99252636725571 19.825894624106542 9 26.329837266176064 16.86153934984699 19.89615171851165 36.912471665465304 10-11 25.47565697937563 26.354306942403365 26.21468310954406 21.95535296867695 12-13 26.491555982707787 23.61984972685229 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22.416467369236674 38-39 22.302393288324325 26.00520531129885 29.126249987193948 22.56615141318288 40-41 22.72399595966934 25.828987276870226 29.00134072290997 22.445676040550463 42-43 22.374781134746488 25.8366007897776 28.766205342174867 23.02241273330105 44-45 22.47109939303545 26.017216847858858 28.975671359510724 22.536012399594966 46-47 22.97949389911136 26.390347911765748 28.224875160414907 22.40528302870798 48-49 22.769976661842577 26.32539035070564 28.196144826956782 22.708488160495 50-51 22.688907763057166 26.617955480555572 28.23178999756002 22.461346758827244 52-53 22.98757285025396 26.56021542627992 27.783722519924975 22.668489203541142 54-55 22.602483939883292 26.584312586028027 27.947350383412584 22.8658530906761 56-57 22.81249541630007 26.71858771519525 27.796883479844876 22.672033388659806 58-59 22.985943090657475 26.329068950937717 27.719230604463863 22.965757353940944 60-61 22.56120445753219 26.64158699470468 28.168927841695396 22.628280706067734 62-63 22.45003157077161 26.686521795007906 28.23917048030412 22.62427615391636 64-65 22.236183829432154 26.696765998185846 28.05915189173181 23.007898280650192 66-67 22.387836955942213 26.687681267227443 27.762252366587205 23.16222941024314 68-69 22.51637280711251 26.392203728965168 28.110402625888835 22.98102083803348 70-71 22.638501626965724 26.51558329561604 27.657346304357137 23.1885687730611 72-73 22.554300122697672 26.550286255765275 27.739592233959083 23.155821387577966 74-75 22.379742367602987 27.210792174918836 27.320428431202725 23.08903702627545 76-77 22.528795523841985 26.589155300257595 27.741278923576377 23.140770252324035 78-79 22.566373123681057 26.671225337080852 27.791941164747275 22.970460374490816 80-81 22.761890326881108 26.758017084083313 27.46733552047224 23.012757068563335 82-83 22.621578510755356 26.817365122375364 27.6033213326215 22.95773503424778 84-85 22.536550543278455 26.90268532085292 27.482972416630442 23.077791719238185 86-87 22.675379429392585 26.870790747012457 27.322315565860038 23.13151425773492 88-89 22.5014388449009 27.065138231552517 26.991286839551563 23.442136083995017 90-91 22.587962747380676 26.75124563445867 27.296414435389988 23.364377182770664 92-93 22.632983726617606 26.576862256444066 27.347552287344314 23.442601729594013 94-95 22.420538142087594 26.54443622062387 27.380037442571332 23.654988194717202 96-97 22.458581396383003 26.356967258820553 27.465623123593314 23.718828221203133 98-99 22.279512152418842 26.561123435197963 27.024231265680616 24.13513314670258 100-101 22.731910134124558 25.744287925437103 27.230675241996018 24.29312669844232 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1122.0 1 776.5 2 603.5 3 774.0 4 620.5 5 585.5 6 1152.0 7 2494.5 8 4149.5 9 4866.5 10 3865.0 11 2652.5 12 2438.5 13 2571.0 14 2797.0 15 2973.0 16 3038.0 17 3229.5 18 3507.5 19 3735.5 20 3963.0 21 4565.0 22 5575.0 23 6658.5 24 7880.5 25 9685.5 26 12496.0 27 15427.5 28 17790.5 29 20214.5 30 23157.5 31 26949.0 32 31918.5 33 34577.5 34 35622.0 35 37979.0 36 41757.5 37 45745.5 38 48523.0 39 51173.0 40 54196.0 41 58261.5 42 61010.5 43 63364.0 44 67032.0 45 71152.0 46 73885.0 47 78618.5 48 83997.0 49 83558.0 50 80324.0 51 77842.0 52 77328.5 53 79001.5 54 81573.0 55 77974.5 56 70372.5 57 65007.5 58 60842.5 59 54450.5 60 44761.5 61 36674.0 62 30278.0 63 23171.5 64 16944.0 65 12514.5 66 9128.5 67 6994.0 68 5294.0 69 3858.0 70 2868.0 71 1951.5 72 1331.5 73 981.0 74 734.0 75 518.0 76 310.0 77 160.5 78 105.5 79 66.0 80 30.0 81 18.0 82 11.5 83 4.5 84 2.0 85 1.5 86 2.0 87 1.5 88 0.5 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.015086917407508815 3 0.0025144862345848023 4 0.0017228887162895868 5 0.11608544782999838 6 9.312911979943713E-5 7 0.0 8 2.793873593983114E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.3282279949859283E-5 16-17 2.3282279949859283E-5 18-19 0.0 20-21 0.0 22-23 1.1641139974929642E-4 24-25 3.725164791977485E-4 26-27 0.005401488948367354 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.01005794493833921 36-37 5.587747187966228E-4 38-39 0.006239651026562288 40-41 0.009638863899241743 42-43 0.005401488948367354 44-45 0.0030965432333312846 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.3282279949859283E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 6.984683984957784E-5 68-69 7.683152383453564E-4 70-71 0.0 72-73 4.6564559899718565E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 6.984683984957784E-5 82-83 0.002072122915537476 84-85 0.003934705311526219 86-87 0.0025610507944845208 88-89 0.0 90-91 0.0026076153543842396 92-93 0.0 94-95 2.3282279949859283E-5 96-97 2.3282279949859283E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2147556.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.96311747406873 #Duplication Level Percentage of deduplicated Percentage of total 1 76.12278254509853 39.555770918439435 2 13.342071412186987 13.86591248277773 3 4.22474761212379 6.5859316940123955 4 1.93621713437654 4.024475136356514 5 1.0680590798064886 2.774983971661516 6 0.6533459465001297 2.036993530151572 7 0.4556091313634358 1.6572409570707638 8 0.33063961468807895 1.3744852111693973 9 0.25425146472998517 1.1890528856746436 >10 1.4505393114530072 14.095361060467274 >50 0.10570340790627085 3.7393077915094906 >100 0.04923363028966602 4.566556949046487 >500 0.004741902661585726 1.7801994885254424 >1k 0.0017893972307870582 1.5047383626861215 >5k 1.7893972307870583E-4 0.7276357941790919 >10k+ 8.946986153935291E-5 0.5213537662720807 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11214 0.5221749747154439 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9776 0.45521513757964865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5875 0.27356678941084656 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3357 0.15631722758335523 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2583 0.12027625822097306 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 0.0 0.0 20-21 0.0 0.0 0.0 4.6564559899718565E-5 0.0 22-23 0.0 0.0 0.0 2.561050794484521E-4 2.3282279949859283E-5 24-25 0.0 0.0 0.0 4.6564559899718567E-4 9.312911979943713E-5 26-27 0.0 0.0 0.0 9.545734779442306E-4 9.312911979943713E-5 28-29 0.0 0.0 0.0 0.003632035672178048 9.312911979943713E-5 30-31 0.0 0.0 0.0 0.009778557578940899 9.312911979943713E-5 32-33 0.0 0.0 0.0 0.02337540906965872 1.396936796991557E-4 34-35 0.0 0.0 0.0 0.04502792942302786 1.396936796991557E-4 36-37 4.6564559899718565E-5 0.0 0.0 0.080905922825761 1.396936796991557E-4 38-39 4.6564559899718565E-5 0.0 0.0 0.13382654515179115 1.396936796991557E-4 40-41 4.6564559899718565E-5 0.0 0.0 0.1886330321537599 1.396936796991557E-4 42-43 4.6564559899718565E-5 0.0 0.0 0.24732765990735517 1.396936796991557E-4 44-45 4.6564559899718565E-5 0.0 0.0 0.3153119173609442 1.396936796991557E-4 46-47 4.6564559899718565E-5 0.0 0.0 0.3972655427844489 1.396936796991557E-4 48-49 4.6564559899718565E-5 0.0 0.0 0.48054625816509555 1.396936796991557E-4 50-51 4.6564559899718565E-5 0.0 0.0 0.5736753779645327 1.396936796991557E-4 52-53 4.6564559899718565E-5 0.0 0.0 0.660657975857207 1.396936796991557E-4 54-55 4.6564559899718565E-5 0.0 0.0 0.7501084954245663 1.396936796991557E-4 56-57 4.6564559899718565E-5 0.0 0.0 0.8635863279001805 1.396936796991557E-4 58-59 4.6564559899718565E-5 0.0 0.0 0.9786473554123851 1.396936796991557E-4 60-61 4.6564559899718565E-5 0.0 0.0 1.1050468532601712 1.396936796991557E-4 62-63 4.6564559899718565E-5 0.0 0.0 1.2259750153197402 1.396936796991557E-4 64-65 4.6564559899718565E-5 0.0 0.0 1.3557737260402056 1.396936796991557E-4 66-67 4.6564559899718565E-5 0.0 0.0 1.5046173417596562 1.396936796991557E-4 68-69 4.6564559899718565E-5 0.0 0.0 1.6657540012926322 1.396936796991557E-4 70-71 4.6564559899718565E-5 0.0 0.0 1.8379264615218416 1.396936796991557E-4 72-73 4.6564559899718565E-5 0.0 0.0 2.011635552227742 2.793873593983114E-4 74-75 4.6564559899718565E-5 0.0 0.0 2.1858102885326387 2.793873593983114E-4 76-77 4.6564559899718565E-5 0.0 0.0 2.372254786371112 2.793873593983114E-4 78-79 4.6564559899718565E-5 2.3282279949859283E-5 0.0 2.5831223958769876 2.793873593983114E-4 80-81 4.6564559899718565E-5 4.6564559899718565E-5 0.0 2.805584580797893 3.4923419924788926E-4 82-83 4.6564559899718565E-5 4.6564559899718565E-5 0.0 3.0353108370631547 4.656455989971856E-4 84-85 4.6564559899718565E-5 4.6564559899718565E-5 0.0 3.265060375608366 4.656455989971856E-4 86-87 4.6564559899718565E-5 4.6564559899718565E-5 0.0 3.4962766977904183 4.656455989971856E-4 88-89 4.6564559899718565E-5 4.6564559899718565E-5 0.0 3.748470354207294 4.656455989971856E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8915 0.0 34.301155 1 GTATCAA 12675 0.0 28.928476 1 TATCAAC 15350 0.0 23.701303 2 ATCAACG 15320 0.0 23.68176 3 TCAACGC 15545 0.0 23.33845 4 CAACGCA 15730 0.0 23.033197 5 AACGCAG 16110 0.0 22.553732 6 GAGTACT 9635 0.0 22.527708 12-13 GTACGCT 130 2.921902E-4 21.946041 3 TAAGGTG 1195 0.0 21.883804 5 GTACTTT 9930 0.0 21.738878 14-15 TACATGG 7960 0.0 21.090204 2 GTACATG 8235 0.0 20.96342 1 TACCTGG 1545 0.0 20.931463 2 TAAGGTA 685 0.0 20.824223 4 GTAAGGT 1150 0.0 20.673325 4 AGAGTAC 13835 0.0 20.666637 10-11 ACGCAGA 17575 0.0 20.538597 7 CGCAGAG 17610 0.0 20.443836 8 AGTACTT 10020 0.0 20.0031 12-13 >>END_MODULE