FastQCFastQC Report
Thu 26 May 2016
SRR938126_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938126_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158011
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120360.5577358039416852No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99240.45986790614134965No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.27015617621967636No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35760.1657081451392046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25170.11663517933875221No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91800.032.7053571
GTATCAA132300.030.9916151
TATCAAC158900.025.6916772
ATCAACG160450.025.354633
TCAACGC161050.025.2601684
CAACGCA163100.024.9718115
AACGCAG166950.024.3931146
TAAGGTG11900.023.5628745
GAGTACT96450.023.36930312-13
GTAAGGT12150.022.686894
GTACTTT100900.022.29440514-15
GTACATG84100.022.2485121
ACGCAGA182250.022.1530197
CGCAGAG181850.022.097278
GTCTACG1302.8472315E-422.0302181
TACATGG83650.021.4757842
AGAGTAC141400.021.33048810-11
AGTACTT100350.020.92264612-13
GCAGAGT190700.020.4989059
GGTAAGG12800.020.049663
Produced by FastQC (version 0.11.5)