##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938126_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158011 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.79029068897239 33.0 31.0 34.0 30.0 34.0 2 31.981446804488023 33.0 31.0 34.0 30.0 34.0 3 32.05382224650384 34.0 31.0 34.0 30.0 34.0 4 35.58211844147226 37.0 35.0 37.0 33.0 37.0 5 35.58372176972221 37.0 35.0 37.0 33.0 37.0 6 35.6075705823557 37.0 35.0 37.0 33.0 37.0 7 35.55535583460881 37.0 35.0 37.0 33.0 37.0 8 35.51969938985482 37.0 35.0 37.0 33.0 37.0 9 37.1395687973787 39.0 37.0 39.0 34.0 39.0 10-11 37.04626389763537 39.0 37.0 39.0 33.0 39.0 12-13 36.93442781338928 39.0 37.0 39.0 33.0 39.0 14-15 38.08864621171996 40.0 38.0 41.0 33.0 41.0 16-17 37.94312424728141 40.0 38.0 41.0 33.0 41.0 18-19 38.09217770437685 40.0 38.0 41.0 33.0 41.0 20-21 38.07110922974906 40.0 38.0 41.0 33.0 41.0 22-23 37.981188928137996 40.0 38.0 41.0 33.0 41.0 24-25 37.864791699393564 40.0 38.0 41.0 33.0 41.0 26-27 37.669876335199405 40.0 38.0 41.0 32.0 41.0 28-29 37.45729447162225 40.0 37.0 41.0 32.0 41.0 30-31 37.23306090654774 40.0 37.0 41.0 31.0 41.0 32-33 36.95561213543397 40.0 36.0 41.0 31.0 41.0 34-35 36.70269243298574 39.0 36.0 41.0 30.0 41.0 36-37 36.42903835986007 39.0 35.0 41.0 30.0 41.0 38-39 36.12592081319326 39.0 35.0 40.5 29.5 41.0 40-41 35.909106811781776 39.0 35.0 40.0 29.0 41.0 42-43 35.72063997820215 39.0 35.0 40.0 27.5 41.0 44-45 35.42428629881868 39.0 35.0 40.0 26.5 41.0 46-47 35.0001946236604 38.0 34.5 40.0 24.5 41.0 48-49 34.57561777952013 38.0 34.0 40.0 23.5 41.0 50-51 33.854071874517786 37.5 33.5 39.5 22.5 40.5 52-53 33.9844004502294 38.0 33.5 40.0 23.0 40.5 54-55 34.08595947842713 38.0 34.0 40.0 22.5 41.0 56-57 33.76638070890278 38.0 33.0 40.0 20.5 41.0 58-59 33.42913590338511 37.5 33.0 40.0 18.5 41.0 60-61 32.98400193511525 37.0 32.5 40.0 16.0 41.0 62-63 32.435922476762165 36.0 31.5 39.0 10.5 41.0 64-65 31.89857883022839 35.5 31.0 39.0 7.5 40.0 66-67 31.34262244261035 35.0 30.5 38.5 4.5 40.0 68-69 30.720343408814877 35.0 30.0 38.0 2.0 40.0 70-71 30.153144956165654 35.0 29.0 37.0 2.0 39.5 72-73 29.504575741272866 34.0 28.5 36.5 2.0 39.0 74-75 28.819546100552778 34.0 27.5 36.0 2.0 39.0 76-77 28.142102612081217 34.0 26.0 35.5 2.0 38.0 78-79 27.410507638746978 33.0 25.5 35.0 2.0 37.0 80-81 26.733309515104416 33.0 24.5 35.0 2.0 36.5 82-83 25.998599173034798 32.0 22.0 35.0 2.0 36.0 84-85 25.344003575514677 32.0 19.5 35.0 2.0 35.5 86-87 24.65608748055501 31.0 16.5 34.0 2.0 35.0 88-89 23.82811348042248 31.0 7.5 34.0 2.0 35.0 90-91 22.990600140592427 30.0 2.0 34.0 2.0 35.0 92-93 22.07939579548019 29.5 2.0 34.0 2.0 35.0 94-95 21.116605522400025 29.0 2.0 34.0 2.0 35.0 96-97 19.912303968793488 28.0 2.0 34.0 2.0 35.0 98-99 18.44307860339915 25.5 2.0 33.0 2.0 35.0 100-101 14.956080622387931 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.3625786521749461 1101 2 0.295619991584644 1101 3 0.28936997567009826 1101 4 0.24510228050502292 1101 5 0.32263714634926544 1101 6 0.3459852697902619 1101 7 0.3062832869916363 1101 8 0.25189979714094335 1101 9 0.34761657302237126 1101 10-11 0.42832139804045966 1101 12-13 0.42304107708240224 1101 14-15 0.5229189728803405 1101 16-17 0.6607625904711156 1101 18-19 0.5394438258294443 1101 20-21 0.5833913333954115 1101 22-23 0.6208583022082195 1101 24-25 0.5582417992685507 1101 26-27 0.6959232961125394 1101 28-29 0.8151633794274815 1101 30-31 0.7150401336801693 1101 32-33 0.7357889715401527 1101 34-35 0.8017040373942663 1101 36-37 0.7360203874078195 1101 38-39 0.8243360961048509 1101 40-41 0.8193405352998866 1101 42-43 1.1031798806775726 1101 44-45 0.9134188429288415 1101 46-47 1.0066057912778135 1101 48-49 1.0647172327334644 1101 50-51 1.1300332976300282 1101 52-53 0.935756083044204 1101 54-55 0.9704069463046139 1101 56-57 1.1970162367055437 1101 58-59 1.158306225509861 1101 60-61 1.1675840899379608 1101 62-63 0.9672397241591426 1101 64-65 1.0672543319132863 1101 66-67 1.0044252924962684 1101 68-69 1.4128467729894751 1101 70-71 1.710268828821274 1101 72-73 1.840261911501365 1101 74-75 1.857969626279477 1101 76-77 1.915835568604443 1101 78-79 1.992802803393559 1101 80-81 2.1484147573329864 1101 82-83 2.2474205656915593 1101 84-85 2.4302606047554143 1101 86-87 2.6491141887852585 1101 88-89 2.979890276795274 1101 90-91 3.2650028072634605 1101 92-93 3.5165278992543136 1101 94-95 3.805381566592658 1101 96-97 4.11044162611412 1101 98-99 4.115145476724354 1101 100-101 4.106148367319413 1102 1 0.3350319773622772 1102 2 0.2468969704622026 1102 3 0.16911947512095082 1102 4 0.10954861624567513 1102 5 0.10349027703438196 1102 6 0.2548736177902171 1102 7 0.14821185371400247 1102 8 0.28322761231810034 1102 9 0.35725292685739163 1102 10-11 0.2571909002356918 1102 12-13 0.2542740642649619 1102 14-15 0.32795623704232924 1102 16-17 0.3740997136571096 1102 18-19 0.23078632668569554 1102 20-21 0.31402074842402783 1102 22-23 0.3633664005679691 1102 24-25 0.11364293829352334 1102 26-27 0.28534054092232 1102 28-29 0.2773760707510533 1102 30-31 0.42016563715611 1102 32-33 0.3389415735517147 1102 34-35 0.41445719111253965 1102 36-37 0.43695636122298964 1102 38-39 0.44034724349908316 1102 40-41 0.5885690007961628 1102 42-43 0.4813613013604794 1102 44-45 0.5383413170274309 1102 46-47 0.5953935720522665 1102 48-49 0.6198480180443013 1102 50-51 0.6566463945113981 1102 52-53 0.5943216673703304 1102 54-55 0.7088544298914456 1102 56-57 0.7103683886176455 1102 58-59 0.7440073999830261 1102 60-61 0.9162140799787153 1102 62-63 0.7063772495269234 1102 64-65 0.83957648245865 1102 66-67 0.7721107271014382 1102 68-69 1.1535187530731434 1102 70-71 1.1227785008411075 1102 72-73 1.3809987438052858 1102 74-75 1.2871025123448518 1102 76-77 1.3922340705236032 1102 78-79 1.6154972416214086 1102 80-81 1.8112744189797283 1102 82-83 1.9879882703749132 1102 84-85 2.1188899032569495 1102 86-87 2.072453528163841 1102 88-89 2.4026908145622983 1102 90-91 2.5675663213281155 1102 92-93 2.816354796644596 1102 94-95 3.409671260534825 1102 96-97 3.4669138885853776 1102 98-99 3.6973573730500995 1102 100-101 3.8053911591122045 1103 1 0.5743148893195382 1103 2 0.5081261786824811 1103 3 0.5367438963652518 1103 4 0.42868034793063714 1103 5 0.5005780801885678 1103 6 0.4832013507450057 1103 7 0.5394956281894565 1103 8 0.49124423975217013 1103 9 0.5405440327374933 1103 10-11 0.6117757235093038 1103 12-13 0.7317497610179444 1103 14-15 0.8968259189288474 1103 16-17 0.9043417578252289 1103 18-19 0.788068915198636 1103 20-21 0.8829293634234574 1103 22-23 0.8257749826239973 1103 24-25 0.8973635063575927 1103 26-27 0.9322291862296836 1103 28-29 1.0072521436578015 1103 30-31 0.9621115740365482 1103 32-33 1.1419100736685124 1103 34-35 1.1556747792960422 1103 36-37 1.2945090856424386 1103 38-39 1.2293297715159497 1103 40-41 1.3084512431038746 1103 42-43 1.453685847789714 1103 44-45 1.39472005834984 1103 46-47 1.3333258044769565 1103 48-49 1.515866658020819 1103 50-51 1.5736619617668595 1103 52-53 1.5274076256939324 1103 54-55 1.5520078922431253 1103 56-57 1.4206042034876987 1103 58-59 1.5183540723998163 1103 60-61 1.6115564817324994 1103 62-63 1.753303630005263 1103 64-65 1.9377927469163119 1103 66-67 2.0815990262035804 1103 68-69 2.103684368545764 1103 70-71 2.3620314642499665 1103 72-73 2.31625597188744 1103 74-75 2.3787720241236165 1103 76-77 2.4123295464958794 1103 78-79 2.6981732043174986 1103 80-81 2.6961791526472894 1103 82-83 2.6469970932172693 1103 84-85 2.7750056963244596 1103 86-87 3.007333404536233 1103 88-89 3.326477748609605 1103 90-91 3.3182805942073053 1103 92-93 3.5534305005852254 1103 94-95 3.8060552728017534 1103 96-97 4.234719798032948 1103 98-99 4.435100929619914 1103 100-101 4.376923266950939 1104 1 -0.024899091662746287 1104 2 0.05778038247140671 1104 3 0.15418665221189798 1104 4 0.07237713625143272 1104 5 0.11184227032790517 1104 6 0.07415938091737928 1104 7 0.0036022021432486895 1104 8 0.04112397838393633 1104 9 0.07485069077250728 1104 10-11 0.015703038816475612 1104 12-13 0.06565710488521859 1104 14-15 0.1508396223915156 1104 16-17 0.20866463316234984 1104 18-19 0.11923473546485042 1104 20-21 0.19502903549522443 1104 22-23 0.15652756115783717 1104 24-25 0.21594078557058083 1104 26-27 0.25149539271357213 1104 28-29 0.28406650269035794 1104 30-31 0.23723442174780018 1104 32-33 0.2389267377416715 1104 34-35 0.33770976843339895 1104 36-37 0.36363178949283537 1104 38-39 0.36883654085685436 1104 40-41 0.438140798834624 1104 42-43 0.43324514067063546 1104 44-45 0.5439794185473232 1104 46-47 0.3886142239935424 1104 48-49 0.5402082295372921 1104 50-51 0.399232253984934 1104 52-53 0.42761863116380283 1104 54-55 0.6290902279358335 1104 56-57 0.7476261434140454 1104 58-59 0.6806870025215588 1104 60-61 0.7551565508544869 1104 62-63 0.8416847741461027 1104 64-65 0.8808007799114641 1104 66-67 0.8945820669577564 1104 68-69 1.0442401758100495 1104 70-71 1.3871417742873042 1104 72-73 1.4831112051698518 1104 74-75 1.6823830461575398 1104 76-77 1.7275474455370876 1104 78-79 1.6745909143335673 1104 80-81 1.7143981839479139 1104 82-83 1.8877745952139833 1104 84-85 2.147502281062085 1104 86-87 2.2928824385908477 1104 88-89 2.490622981585375 1104 90-91 2.7299974588545943 1104 92-93 3.057310444978132 1104 94-95 3.2090754336679908 1104 96-97 3.413174314074432 1104 98-99 3.7598761183799674 1104 100-101 3.904712340575884 1105 1 0.2784136016412333 1105 2 0.2682439178058402 1105 3 0.17133851877103723 1105 4 0.07164068856748429 1105 5 0.1365195492057012 1105 6 0.22644159979959255 1105 7 0.3012929837893239 1105 8 0.3118207033794249 1105 9 0.30009581413507647 1105 10-11 0.37870611651798214 1105 12-13 0.2713406615463114 1105 14-15 0.3411226500665663 1105 16-17 0.4490154898351264 1105 18-19 0.3920134413833054 1105 20-21 0.2899073234363101 1105 22-23 0.34274583082201815 1105 24-25 0.37085602152517083 1105 26-27 0.3858210676407978 1105 28-29 0.3452703953795364 1105 30-31 0.4813487426878069 1105 32-33 0.47386004698883255 1105 34-35 0.5642016544044424 1105 36-37 0.46402628718894334 1105 38-39 0.47264263244884575 1105 40-41 0.6150692141703331 1105 42-43 0.618207767033681 1105 44-45 0.6137627101522298 1105 46-47 0.5910378083407366 1105 48-49 0.6893447337977463 1105 50-51 0.6464261175562598 1105 52-53 0.6569219061683143 1105 54-55 0.6874199169361432 1105 56-57 0.6996242667720622 1105 58-59 0.7506913367327783 1105 60-61 0.8262507193368194 1105 62-63 0.8820125410179571 1105 64-65 0.9493791131440723 1105 66-67 1.031441230218892 1105 68-69 1.2569182526505642 1105 70-71 1.1460671411627956 1105 72-73 1.0594202896635743 1105 74-75 1.2412472012343585 1105 76-77 1.375184628953928 1105 78-79 1.636883385000747 1105 80-81 1.5669387226853893 1105 82-83 1.8812836752890227 1105 84-85 1.7569581177023927 1105 86-87 1.88322269258299 1105 88-89 2.1169003663383563 1105 90-91 2.31809761227397 1105 92-93 2.405866151938671 1105 94-95 2.780554396065188 1105 96-97 2.6987072108675747 1105 98-99 2.906342891484986 1105 100-101 2.8833965121707976 1106 1 0.2694720605300276 1106 2 0.2031082587214783 1106 3 0.15646214020356553 1106 4 0.24367157131333528 1106 5 0.2828016112287841 1106 6 0.06329494593752116 1106 7 0.16681724061646008 1106 8 0.2079674043417299 1106 9 0.17102638547754623 1106 10-11 0.24857307712343157 1106 12-13 0.3775177108961003 1106 14-15 0.5110796638119126 1106 16-17 0.30231254765461557 1106 18-19 0.3634777875508277 1106 20-21 0.35885143213530313 1106 22-23 0.288310573006612 1106 24-25 0.31923906644253464 1106 26-27 0.3571059744344396 1106 28-29 0.4025492775118309 1106 30-31 0.33154291651433 1106 32-33 0.3817284531128635 1106 34-35 0.38165158123235443 1106 36-37 0.3510538833390626 1106 38-39 0.21404527731436218 1106 40-41 0.5576576802825457 1106 42-43 0.36725836393532063 1106 44-45 0.4754829981388795 1106 46-47 0.6649877753914168 1106 48-49 0.5435571199912985 1106 50-51 0.6888071550868133 1106 52-53 0.7272747494174965 1106 54-55 0.28582188530896957 1106 56-57 0.6377070539719725 1106 58-59 0.8067980275080942 1106 60-61 0.7946457352154326 1106 62-63 0.6505464817358586 1106 64-65 0.889190332919167 1106 66-67 0.9597796883073961 1106 68-69 0.794375336212628 1106 70-71 0.986449044837407 1106 72-73 1.4333535007030385 1106 74-75 1.4087155096566732 1106 76-77 1.5152834310658534 1106 78-79 1.731059462335157 1106 80-81 1.9750103267066663 1106 82-83 2.041116015140947 1106 84-85 2.0664770742947347 1106 86-87 1.8862330433796721 1106 88-89 2.1726145204578096 1106 90-91 2.4745221670830126 1106 92-93 2.680428401145573 1106 94-95 2.886916511807545 1106 96-97 2.98119605373995 1106 98-99 3.250432271049906 1106 100-101 3.4215209184682216 1107 1 0.3330852130537991 1107 2 0.20457404231470377 1107 3 0.041652643668363964 1107 4 0.13155284042216664 1107 5 0.15300675639540628 1107 6 0.18485535606651382 1107 7 0.26236100034734733 1107 8 0.2339223417817351 1107 9 0.27171289171740654 1107 10-11 0.261489437914328 1107 12-13 0.22504031881310027 1107 14-15 0.3072829051539472 1107 16-17 0.45216108872801897 1107 18-19 0.3981558314517386 1107 20-21 0.5673236532370964 1107 22-23 0.5282098155103014 1107 24-25 0.6174378951063915 1107 26-27 0.5536423850797192 1107 28-29 0.5805359023373811 1107 30-31 0.6585800491608822 1107 32-33 0.5753151457016585 1107 34-35 0.6292405406631474 1107 36-37 0.6105568721823289 1107 38-39 0.7757418996391223 1107 40-41 0.6598450617976965 1107 42-43 0.9051825901032302 1107 44-45 0.8046821010825482 1107 46-47 0.8979578224223417 1107 48-49 0.8807710421119666 1107 50-51 0.7060867833569944 1107 52-53 0.6425636305978628 1107 54-55 0.890312208878477 1107 56-57 0.9370588662355033 1107 58-59 0.9475408821155966 1107 60-61 1.0143406392578385 1107 62-63 1.00109806650849 1107 64-65 1.1827257997192113 1107 66-67 1.4540419966140412 1107 68-69 1.5690359149430364 1107 70-71 1.5609057287944559 1107 72-73 1.6575802736396845 1107 74-75 1.8850562539230182 1107 76-77 2.024616003407157 1107 78-79 2.0015122660395477 1107 80-81 2.245324578769811 1107 82-83 2.323544561215922 1107 84-85 2.43459244929171 1107 86-87 2.484717244055691 1107 88-89 2.6914446363813695 1107 90-91 2.956578620322336 1107 92-93 3.1788079318044105 1107 94-95 3.410527163544785 1107 96-97 3.2645402103698586 1107 98-99 3.3970535874316674 1107 100-101 3.8202221228792865 1108 1 0.2558803800399403 1108 2 0.1751451505945738 1108 3 0.11102812431067122 1108 4 0.04087701429129709 1108 5 0.046580592619292815 1108 6 0.1443025361069914 1108 7 0.1518264687081654 1108 8 0.05614647700817699 1108 9 0.11107295354570823 1108 10-11 0.06235765487961231 1108 12-13 0.07101382753066332 1108 14-15 0.14006012816049918 1108 16-17 0.23280598158064691 1108 18-19 0.15836448282201587 1108 20-21 0.17890029392516738 1108 22-23 0.223957589729892 1108 24-25 0.21588118792229238 1108 26-27 0.07366522635010853 1108 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2316 54-55 -1.6485601347261394 2316 56-57 -1.5462712976477064 2316 58-59 -1.5361140938079103 2316 60-61 -1.642339316460422 2316 62-63 -1.6257995285580407 2316 64-65 -1.59681664487767 2316 66-67 -1.835648574498233 2316 68-69 -1.8377384451110288 2316 70-71 -2.006201321647339 2316 72-73 -1.9008052424260313 2316 74-75 -1.8529161922359485 2316 76-77 -1.9689735007323748 2316 78-79 -2.559627445585768 2316 80-81 -2.730122313697535 2316 82-83 -2.5714280683183723 2316 84-85 -2.5524408774763323 2316 86-87 -2.5293486623957264 2316 88-89 -2.843507380407665 2316 90-91 -2.7553645469091705 2316 92-93 -3.0659718140509717 2316 94-95 -3.152692839307722 2316 96-97 -3.237015933043228 2316 98-99 -3.2888358508285744 2316 100-101 -2.8966861011646152 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6593.0 3 2142.0 4 2312.0 5 2555.0 6 2919.0 7 3663.0 8 4507.0 9 5573.0 10 6823.0 11 8956.0 12 10703.0 13 12902.0 14 15189.0 15 18346.0 16 22198.0 17 27430.0 18 26449.0 19 22289.0 20 22465.0 21 23847.0 22 25645.0 23 28537.0 24 32157.0 25 35562.0 26 40364.0 27 46212.0 28 53037.0 29 61353.0 30 72259.0 31 86346.0 32 105330.0 33 132122.0 34 171457.0 35 231840.0 36 297525.0 37 323015.0 38 158737.0 39 8651.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.102859393190656 18.319117906503564 14.861517707644303 22.71650499266148 2 15.750714361209955 19.685217497723873 39.97757248842466 24.586495652641513 3 19.57296809106453 23.728213982367187 29.48173479568712 27.217083130881157 4 12.981565452842611 16.604909079107205 36.416298068452626 33.997227399597556 5 13.96554514715451 36.888684214553734 33.78336953008226 15.362401108209497 6 32.40890089311304 35.65115444297999 16.888916920731 15.051027743175974 7 28.9784551678993 32.8680036550138 19.801097524057308 18.352443653029596 8 26.870010527696742 34.005294907626535 19.1719509894227 19.952743575254026 9 26.533682390787032 16.937183102639857 19.923209832701836 36.60592467387127 10-11 25.43799115290208 26.28308534368794 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22.146951223187216 36-37 22.802591856338058 25.53422151591237 29.236939655012556 22.426246972737015 38-39 22.2460129262396 26.024949570608563 29.132962096379465 22.59607540677237 40-41 22.685337860151446 25.88121737417512 29.07535877544162 22.358085990231807 42-43 22.376658761759156 25.86990482825076 28.781001153870843 22.972435256119237 44-45 22.502512509248838 26.00185799392734 28.936390009099956 22.55923948772387 46-47 22.983842371929974 26.396045670943202 28.244816872644478 22.375295084482346 48-49 22.835478346961718 26.32891537700489 28.169865704602966 22.665740571430426 50-51 22.712166048334208 26.680331002903575 28.255630455789422 22.351872492972795 52-53 22.968822488259363 26.566093998313928 27.859290338637337 22.60579317478937 54-55 22.557227647425368 26.60521630559539 27.99043322759459 22.847122819384648 56-57 22.792333677990932 26.682105967612692 27.89101064700002 22.63454970739636 58-59 22.964359328781455 26.32154116560671 27.80394097002967 22.91015853558216 60-61 22.500181911128188 26.61649472782156 28.290447881418974 22.59287547963128 62-63 22.415159119448905 26.623793288273955 28.395402173129693 22.565645419147444 64-65 22.230683061641514 26.71291444917232 28.13911690155924 22.917285587626925 66-67 22.40145183867666 26.60897097087309 27.886652033039894 23.102925157410354 68-69 22.4939219206638 26.33669083686092 28.233477959025915 22.935909283449366 70-71 22.627147571618163 26.455194922416407 27.853829217610627 23.063828288354802 72-73 22.564404527938812 26.455429594092333 27.94439651466709 23.035769363301764 74-75 22.39939139758186 27.13140033011537 27.546651446971715 22.922556825331057 76-77 22.701519572029348 26.52391091622229 27.646553000574887 23.128016511173474 78-79 22.713000494040465 26.661315334070533 27.710270758273325 22.91541341361568 80-81 22.931461911980968 26.711366595184476 27.340825445496876 23.01634604733768 82-83 22.878697539951656 26.730597350931074 27.469369604646428 22.921335504470846 84-85 22.82147792031622 26.872611941699226 27.363843166570227 22.942066971414324 86-87 22.9394879052504 26.807574166201526 27.178738246026708 23.074199682521368 88-89 22.806883774655017 26.875283034697446 26.981706398519346 23.336126792128194 90-91 22.931336059374956 26.67003655658437 27.020236258485852 23.37839112555482 92-93 23.079422225394385 26.397255728201596 27.16542658753148 23.357895458872537 94-95 22.89289795048057 26.3317704482614 27.271170216957497 23.504161384300538 96-97 23.01450786808501 26.151984982270736 27.255857609678092 23.577649539966163 98-99 22.90732870348805 26.316306635825754 26.787147393759557 23.98921726692664 100-101 23.741270116033377 25.158262139927817 26.872704845111706 24.2277628989271 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1142.0 1 764.5 2 582.5 3 784.0 4 616.0 5 563.5 6 1135.5 7 2519.0 8 4261.0 9 5035.0 10 4032.0 11 2787.0 12 2556.5 13 2670.5 14 2865.0 15 3068.0 16 3147.5 17 3245.5 18 3480.0 19 3788.0 20 4157.5 21 4844.5 22 5755.0 23 6777.5 24 8147.0 25 10039.0 26 12491.0 27 15762.5 28 18380.5 29 20396.0 30 23064.5 31 26721.5 32 31689.0 33 34741.5 34 35879.5 35 38101.5 36 42144.5 37 46337.5 38 49121.5 39 51292.0 40 54314.5 41 58289.5 42 60925.5 43 63322.5 44 66705.0 45 70790.5 46 73807.5 47 78065.0 48 83506.5 49 83188.5 50 79558.0 51 77438.0 52 77453.5 53 79253.0 54 80879.0 55 77237.5 56 70630.5 57 65365.0 58 61284.0 59 55030.5 60 45408.0 61 37396.0 62 30917.5 63 23938.0 64 17684.0 65 13269.5 66 9803.5 67 7471.5 68 5786.0 69 4303.0 70 3204.5 71 2277.5 72 1590.5 73 1146.5 74 832.0 75 581.0 76 379.5 77 230.0 78 140.0 79 93.5 80 63.0 81 34.0 82 22.5 83 22.0 84 12.5 85 8.0 86 8.0 87 7.0 88 8.5 89 7.5 90 3.0 91 1.0 92 0.5 93 1.0 94 1.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4521756376589368 2 0.03887839311291741 3 0.052038659673189804 4 0.05518970941297334 5 0.08062980216504921 6 0.08030542939771855 7 0.04513415362572294 8 0.03911008794672502 9 0.04467076395810772 10-11 0.04784498318127201 12-13 0.04344278133892737 14-15 0.03681630909202965 16-17 0.037604071526975535 18-19 0.04654749211194938 20-21 0.0546104723284543 22-23 0.04703405126294537 24-25 0.06415629948132794 26-27 0.05611648874820379 28-29 0.04367447617273498 30-31 0.045945085544049594 32-33 0.044068357390207924 34-35 0.04930466063425997 36-37 0.04388300152316184 38-39 0.024976703084460646 40-41 0.02309997493061898 42-43 0.0227292631965268 44-45 0.014249232279168179 46-47 0.02678392278816002 48-49 0.026204685703640995 50-51 0.015106503164256347 52-53 0.015477214898348525 54-55 0.015500384381729286 56-57 0.014550435563118075 58-59 0.013600486744506864 60-61 0.016867383901194202 62-63 0.0157320792155369 64-65 0.016589350100625067 66-67 0.009059268001877655 68-69 0.011909114457711291 70-71 0.017168587185144097 72-73 0.015778418182298422 74-75 0.01299808017660707 76-77 0.009244623868923744 78-79 0.0135773172611261 80-81 0.016867383901194202 82-83 0.014341910212691222 84-85 0.012488351542230323 86-87 0.003266897156687339 88-89 0.027339990389298293 90-91 0.031070277213600856 92-93 0.016357655266817452 94-95 0.03132514153078923 96-97 0.02599616035321414 98-99 0.022590246296242233 100-101 0.1482846936368721 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2158011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.73276722344069 #Duplication Level Percentage of deduplicated Percentage of total 1 77.34432632818078 41.559246826459734 2 12.649029378249422 13.593347023678778 3 4.103481302529713 6.6147421690371075 4 1.815377970708195 3.901811276905023 5 1.0110321938020685 2.7162778762485567 6 0.6314548951703189 2.0357891336573335 7 0.42088480318184257 1.583071361007752 8 0.3015355623531164 1.2961872145207431 9 0.24024433467627987 1.1618093620719836 >10 1.3362997056034704 13.384429487443898 >50 0.09562233986151393 3.503864095882455 >100 0.04455963461487312 4.26395164277131 >500 0.004158795088562437 1.611405102885414 >1k 0.0017328312869009995 1.4803064760768259 >5k 1.7328312869009996E-4 0.7334260535306575 >10k+ 8.664156434504998E-5 0.5603348978224574 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12036 0.5577358039416852 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9924 0.45986790614134965 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5830 0.27015617621967636 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3576 0.1657081451392046 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2517 0.11663517933875221 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.633896676152253E-5 2 0.0 0.0 0.0 0.0 4.633896676152253E-5 3 0.0 0.0 0.0 0.0 4.633896676152253E-5 4 0.0 0.0 0.0 0.0 4.633896676152253E-5 5 0.0 0.0 0.0 0.0 4.633896676152253E-5 6 0.0 0.0 0.0 0.0 4.633896676152253E-5 7 0.0 0.0 0.0 0.0 4.633896676152253E-5 8 0.0 0.0 0.0 0.0 4.633896676152253E-5 9 0.0 0.0 0.0 0.0 4.633896676152253E-5 10-11 0.0 0.0 0.0 0.0 4.633896676152253E-5 12-13 0.0 0.0 0.0 0.0 4.633896676152253E-5 14-15 0.0 0.0 0.0 6.95084501422838E-5 4.633896676152253E-5 16-17 0.0 0.0 0.0 9.267793352304506E-5 4.633896676152253E-5 18-19 0.0 4.633896676152253E-5 0.0 1.390169002845676E-4 4.633896676152253E-5 20-21 0.0 9.267793352304506E-5 0.0 2.548643171883739E-4 4.633896676152253E-5 22-23 0.0 9.267793352304506E-5 0.0 5.328981177575091E-4 9.267793352304506E-5 24-25 0.0 9.267793352304506E-5 0.0 8.341014017074056E-4 1.390169002845676E-4 26-27 0.0 9.267793352304506E-5 0.0 0.0014596774529879598 1.390169002845676E-4 28-29 0.0 9.267793352304506E-5 0.0 0.003382744573591145 1.390169002845676E-4 30-31 0.0 9.267793352304506E-5 0.0 0.009406810252589074 1.6218638366532887E-4 32-33 0.0 9.267793352304506E-5 0.0 0.024049923749230193 1.8535586704609012E-4 34-35 0.0 9.267793352304506E-5 0.0 0.04564388226009969 1.8535586704609012E-4 36-37 0.0 9.267793352304506E-5 0.0 0.08262237773579467 1.8535586704609012E-4 38-39 0.0 9.267793352304506E-5 0.0 0.1363060707290185 2.085253504268514E-4 40-41 0.0 9.267793352304506E-5 0.0 0.19172747497579945 2.3169483380761267E-4 42-43 0.0 9.267793352304506E-5 0.0 0.24995238671165254 2.3169483380761267E-4 44-45 0.0 9.267793352304506E-5 0.0 0.3183023626848983 2.3169483380761267E-4 46-47 0.0 9.267793352304506E-5 0.0 0.4003455033361739 2.3169483380761267E-4 48-49 0.0 9.267793352304506E-5 0.0 0.48296788107196853 2.3169483380761267E-4 50-51 0.0 9.267793352304506E-5 0.0 0.5720313751876149 2.3169483380761267E-4 52-53 0.0 9.267793352304506E-5 0.0 0.6588010904485658 2.3169483380761267E-4 54-55 0.0 9.267793352304506E-5 0.0 0.7548849380285828 2.3169483380761267E-4 56-57 0.0 9.267793352304506E-5 0.0 0.8664228310235675 2.3169483380761267E-4 58-59 0.0 9.267793352304506E-5 0.0 0.9842164845313578 2.3169483380761267E-4 60-61 0.0 9.267793352304506E-5 0.0 1.1118571684759715 2.3169483380761267E-4 62-63 0.0 9.267793352304506E-5 0.0 1.23294088862383 2.3169483380761267E-4 64-65 0.0 9.267793352304506E-5 0.0 1.361044962236059 2.3169483380761267E-4 66-67 9.267793352304506E-5 9.267793352304506E-5 0.0 1.5071748939185206 2.3169483380761267E-4 68-69 9.267793352304506E-5 9.267793352304506E-5 0.0 1.6691295827500414 2.3169483380761267E-4 70-71 1.1584741690380632E-4 1.1584741690380632E-4 0.0 1.8393326076651138 2.780338005691352E-4 72-73 1.390169002845676E-4 1.8535586704609012E-4 0.0 2.005967532139549 2.780338005691352E-4 74-75 1.390169002845676E-4 1.8535586704609012E-4 0.0 2.1774448786405634 2.780338005691352E-4 76-77 1.390169002845676E-4 1.8535586704609012E-4 0.0 2.360367949931673 2.780338005691352E-4 78-79 1.8535586704609012E-4 1.8535586704609012E-4 0.0 2.57148828249717 2.780338005691352E-4 80-81 2.780338005691352E-4 1.8535586704609012E-4 0.0 2.7914825271974983 3.2437276733065774E-4 82-83 2.780338005691352E-4 1.8535586704609012E-4 0.0 3.0104573146290727 3.7071173409218024E-4 84-85 2.780338005691352E-4 1.8535586704609012E-4 0.0 3.2307991015801125 3.7071173409218024E-4 86-87 2.780338005691352E-4 1.8535586704609012E-4 0.0 3.4629341555719595 4.170507008537028E-4 88-89 2.780338005691352E-4 1.8535586704609012E-4 0.0 3.711079322579913 4.6338966761522534E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9180 0.0 32.705357 1 GTATCAA 13230 0.0 30.991615 1 TATCAAC 15890 0.0 25.691677 2 ATCAACG 16045 0.0 25.35463 3 TCAACGC 16105 0.0 25.260168 4 CAACGCA 16310 0.0 24.971811 5 AACGCAG 16695 0.0 24.393114 6 TAAGGTG 1190 0.0 23.562874 5 GAGTACT 9645 0.0 23.369303 12-13 GTAAGGT 1215 0.0 22.68689 4 GTACTTT 10090 0.0 22.294405 14-15 GTACATG 8410 0.0 22.248512 1 ACGCAGA 18225 0.0 22.153019 7 CGCAGAG 18185 0.0 22.09727 8 GTCTACG 130 2.8472315E-4 22.030218 1 TACATGG 8365 0.0 21.475784 2 AGAGTAC 14140 0.0 21.330488 10-11 AGTACTT 10035 0.0 20.922646 12-13 GCAGAGT 19070 0.0 20.498905 9 GGTAAGG 1280 0.0 20.04966 3 >>END_MODULE