FastQCFastQC Report
Thu 26 May 2016
SRR938126_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938126_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2158011
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114320.5297470680177256No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100400.4652432262856862No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60780.281648239976534No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34120.15810855459031486No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26140.12113005911461991No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA88200.032.8530041
GTATCAA128450.030.7312871
ATCAACG154700.025.360823
TATCAAC155850.025.2369792
TCAACGC156750.025.1194734
CAACGCA158200.024.8291935
AACGCAG162150.024.3122236
TAAGGTG11950.024.2451825
GTAAGGT11150.023.8548494
GTACATG85200.022.9705541
GAGTACT96250.022.8724412-13
TACATGG84750.022.5320222
GTACTTT98650.022.02711114-15
GGACCGA6700.021.9760886
ACGCAGA179150.021.9256367
CGCAGAG178200.021.8292948
AGTACTT97800.021.49007612-13
AGAGTAC138300.021.36149810-11
ACATGGG86900.021.3166243
GCAGAGT189250.020.3539419
Produced by FastQC (version 0.11.5)