##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938126_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2158011 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46738640349841 33.0 31.0 34.0 31.0 34.0 2 32.69891997770169 34.0 31.0 34.0 31.0 34.0 3 32.66044890410661 34.0 31.0 34.0 31.0 34.0 4 36.19815422627595 37.0 35.0 37.0 35.0 37.0 5 36.158769348256335 37.0 35.0 37.0 35.0 37.0 6 36.22054336145645 37.0 37.0 37.0 35.0 37.0 7 36.18267886493628 37.0 37.0 37.0 35.0 37.0 8 36.175455083407826 37.0 37.0 37.0 35.0 37.0 9 37.871283325247184 39.0 38.0 39.0 35.0 39.0 10-11 37.838012410502074 39.0 38.0 39.0 35.0 39.0 12-13 37.77518279563913 39.0 38.0 39.0 35.0 39.0 14-15 39.02621047807449 40.0 38.0 41.0 36.0 41.0 16-17 38.95670643013405 40.0 38.0 41.0 35.5 41.0 18-19 38.9596167489415 40.0 38.0 41.0 35.5 41.0 20-21 38.96767972915801 40.0 38.5 41.0 35.0 41.0 22-23 38.9955028032758 40.0 38.5 41.0 35.0 41.0 24-25 38.942548949009065 40.0 38.0 41.0 35.0 41.0 26-27 38.76346065891231 40.0 38.0 41.0 35.0 41.0 28-29 38.68628681688833 40.0 38.0 41.0 34.5 41.0 30-31 38.4637191376689 40.0 38.0 41.0 34.0 41.0 32-33 38.24771120258423 40.0 38.0 41.0 34.0 41.0 34-35 38.027834658859476 40.0 37.5 41.0 33.5 41.0 36-37 37.94160363408713 40.0 37.0 41.0 33.0 41.0 38-39 37.894187749738066 40.0 37.0 41.0 33.0 41.0 40-41 37.773905693715186 40.0 37.0 41.0 33.0 41.0 42-43 37.576983620565414 40.0 37.0 41.0 33.0 41.0 44-45 37.310402032241726 40.0 37.0 41.0 32.5 41.0 46-47 36.920387569850206 40.0 36.0 41.0 31.0 41.0 48-49 36.51735579661086 39.5 36.0 41.0 30.5 41.0 50-51 36.193302536456024 39.0 35.0 41.0 30.0 41.0 52-53 35.71812539417084 39.0 35.0 40.0 28.0 41.0 54-55 35.3926411867224 38.0 35.0 40.0 28.0 41.0 56-57 35.099353061685036 38.0 34.0 40.0 27.0 41.0 58-59 34.70205110168577 38.0 34.0 40.0 26.0 41.0 60-61 34.263837858101745 37.0 34.0 40.0 26.0 41.0 62-63 33.82615473229747 37.0 33.0 39.5 24.5 41.0 64-65 33.3713396270918 36.0 33.0 39.0 23.0 40.5 66-67 32.83930457259022 35.5 32.0 39.0 22.0 40.0 68-69 32.29083772047501 35.0 31.5 38.0 20.5 40.0 70-71 31.68124004001833 35.0 31.0 37.5 18.5 39.5 72-73 31.20268895756324 35.0 30.5 37.0 17.5 39.0 74-75 30.641231671200934 34.0 30.0 36.0 12.5 39.0 76-77 29.455300274187664 33.0 28.5 35.0 12.0 37.0 78-79 30.14960674435858 34.0 30.0 35.0 9.5 37.0 80-81 29.963619045500693 34.0 30.0 35.0 7.0 37.0 82-83 29.501820194614393 34.0 29.0 35.0 4.0 36.5 84-85 28.981927107878505 34.0 29.0 35.0 2.0 36.0 86-87 28.436662046671678 34.0 28.5 35.0 2.0 36.0 88-89 27.794295534174758 33.0 26.5 35.0 2.0 35.0 90-91 27.246084473156067 33.0 26.0 35.0 2.0 35.0 92-93 26.593578763036888 32.5 25.0 35.0 2.0 35.0 94-95 25.822430932928516 32.0 23.5 34.0 2.0 35.0 96-97 24.966547668200022 32.0 19.0 34.0 2.0 35.0 98-99 23.91859193488819 31.0 4.5 34.0 2.0 35.0 100-101 20.459270596859795 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.05578623024304363 1101 2 0.04737610895531219 1101 3 0.24387276102186206 1101 4 0.12285340954270652 1101 5 -0.029082128111866723 1101 6 -0.00760467808629528 1101 7 0.05686030662701569 1101 8 0.0863325006273925 1101 9 0.11711415839265982 1101 10-11 0.1214857376104348 1101 12-13 0.15819387432841125 1101 14-15 0.3318301322649617 1101 16-17 0.338299887312985 1101 18-19 0.28228932151974107 1101 20-21 0.2691597223672275 1101 22-23 0.14780384817763803 1101 24-25 0.16577861244869752 1101 26-27 0.23202154482011395 1101 28-29 0.2482483482419866 1101 30-31 0.2510203173899441 1101 32-33 0.2893837652024942 1101 34-35 0.11631180050438417 1101 36-37 0.29952996415546096 1101 38-39 0.3334308287003154 1101 40-41 0.39689024523681127 1101 42-43 0.5321637636037551 1101 44-45 0.44857590368845734 1101 46-47 0.5093300280730588 1101 48-49 0.6197467163373673 1101 50-51 0.7414015357125265 1101 52-53 0.6391988735684322 1101 54-55 0.8031180816889503 1101 56-57 0.796577369878257 1101 58-59 0.8512644587921301 1101 60-61 0.9265364499219189 1101 62-63 0.886919200065762 1101 64-65 0.8080267574823381 1101 66-67 0.9563885940132835 1101 68-69 1.0041373212085176 1101 70-71 1.0303153402145355 1101 72-73 1.0588507165022172 1101 74-75 1.1462118157695045 1101 76-77 1.1607451751491844 1101 78-79 1.2080821214400892 1101 80-81 1.3835219916468624 1101 82-83 1.4647077815221472 1101 84-85 1.5226065048393487 1101 86-87 1.5464176763203277 1101 88-89 1.6489073575794215 1101 90-91 2.065603447792739 1101 92-93 2.3885897748529175 1101 94-95 2.285648647970387 1101 96-97 2.4349792575803484 1101 98-99 2.469905962352435 1101 100-101 2.731215263330615 1102 1 0.012705243378476894 1102 2 0.041234750717855206 1102 3 0.2170121802269449 1102 4 0.04801389292317282 1102 5 -0.08935766564747638 1102 6 -0.04726744654099946 1102 7 0.035379029642690796 1102 8 0.042379738745033535 1102 9 0.03218361452157836 1102 10-11 0.13484061211784848 1102 12-13 0.10310991765688726 1102 14-15 0.26363701531261086 1102 16-17 0.2616732591329196 1102 18-19 0.16305598257785903 1102 20-21 0.13796003252529232 1102 22-23 0.16224558273221845 1102 24-25 0.11698303416128653 1102 26-27 0.12176433035094192 1102 28-29 0.13974639096821306 1102 30-31 0.10503312338923365 1102 32-33 0.3095829720560275 1102 34-35 0.14977385814390942 1102 36-37 0.2131391562906444 1102 38-39 0.28564329132588995 1102 40-41 0.24789417503087918 1102 42-43 0.30804774946150815 1102 44-45 0.39577048703063866 1102 46-47 0.44853638065024626 1102 48-49 0.4372265133188833 1102 50-51 0.5140727229764437 1102 52-53 0.5434028602102856 1102 54-55 0.6290293479516933 1102 56-57 0.7501569837380089 1102 58-59 0.60044291565778 1102 60-61 0.7506524368382159 1102 62-63 0.7268730684108533 1102 64-65 0.7238728339230107 1102 66-67 0.6862157963342383 1102 68-69 0.7407109785742492 1102 70-71 0.9440361587573705 1102 72-73 0.8823135528039963 1102 74-75 0.8495334637237733 1102 76-77 0.9074854117862792 1102 78-79 1.0492991378785739 1102 80-81 1.1559838113315486 1102 82-83 1.2527440715395315 1102 84-85 1.3618599855413223 1102 86-87 1.6467779845834585 1102 88-89 1.9513101023911084 1102 90-91 1.950645672523379 1102 92-93 2.087708960818702 1102 94-95 2.1283150380213733 1102 96-97 2.288884384935187 1102 98-99 2.456685992442555 1102 100-101 2.8948697866776882 1103 1 0.29565313889398226 1103 2 0.2836682499661407 1103 3 0.3529306722576351 1103 4 0.17452255104851133 1103 5 0.07128634793718902 1103 6 0.16183930805460278 1103 7 0.2636468655006823 1103 8 0.21532661600989655 1103 9 0.2393437134715839 1103 10-11 0.3173830042881036 1103 12-13 0.23935484886506941 1103 14-15 0.36070325551195026 1103 16-17 0.35023411158839934 1103 18-19 0.4055913068906918 1103 20-21 0.45361773259656246 1103 22-23 0.45242904753968816 1103 24-25 0.526238932527896 1103 26-27 0.4823927706904314 1103 28-29 0.5450640552210331 1103 30-31 0.43523084370573173 1103 32-33 0.5694255624856694 1103 34-35 0.5730846102717564 1103 36-37 0.5633065686761682 1103 38-39 0.787673802906987 1103 40-41 0.6958645619062267 1103 42-43 0.7391840572846675 1103 44-45 0.6836718724504891 1103 46-47 0.6517432712122613 1103 48-49 0.691420807521439 1103 50-51 0.797140412685529 1103 52-53 0.800214657542476 1103 54-55 0.8292413836743222 1103 56-57 0.8529074584225 1103 58-59 1.0829918965086804 1103 60-61 0.9845026821168332 1103 62-63 1.0344746089641816 1103 64-65 0.9690975378816091 1103 66-67 0.9813707201750788 1103 68-69 1.0861644894425808 1103 70-71 1.0214471666458138 1103 72-73 0.9182248571830733 1103 74-75 1.0368376750886483 1103 76-77 0.8028421888432931 1103 78-79 1.2794537322853827 1103 80-81 1.3898190823359649 1103 82-83 1.5036589996929663 1103 84-85 1.6224662639550047 1103 86-87 1.8263494807530414 1103 88-89 1.984825522898678 1103 90-91 1.8313186763028888 1103 92-93 2.0067220962520267 1103 94-95 1.8074822287876344 1103 96-97 2.101274698376699 1103 98-99 2.459057917475352 1103 100-101 2.803164336640851 1104 1 0.05896334845797924 1104 2 0.07431144839983972 1104 3 0.08134746064720133 1104 4 0.028248446833075036 1104 5 -0.02782632863983281 1104 6 0.02089063456916307 1104 7 0.020778222781977718 1104 8 -3.5948992614009967E-4 1104 9 -0.019446485534977853 1104 10-11 0.04334427132966567 1104 12-13 0.012092099626855202 1104 14-15 0.07489687674466694 1104 16-17 0.09129274405630383 1104 18-19 -0.0024410577891202934 1104 20-21 0.03840421815904449 1104 22-23 -0.006142465776186157 1104 24-25 0.04040421145315776 1104 26-27 -0.025341612884439257 1104 28-29 0.011260623595198638 1104 30-31 -0.08829155201878791 1104 32-33 -0.06370304137914928 1104 34-35 0.033895567081117406 1104 36-37 0.12800555130482394 1104 38-39 0.07030965469363082 1104 40-41 0.08927097769905146 1104 42-43 0.07560380771760578 1104 44-45 0.0016106616636761828 1104 46-47 -0.0975968172764965 1104 48-49 -0.09687906383486222 1104 50-51 -0.02596619078553175 1104 52-53 -0.12475302391634813 1104 54-55 -0.09593707399404394 1104 56-57 -0.035009748797016016 1104 58-59 -0.14535597049626148 1104 60-61 -0.0816321865728824 1104 62-63 -0.15143416319489944 1104 64-65 -0.22753001410818285 1104 66-67 -0.18908132100121833 1104 68-69 0.06467878511331548 1104 70-71 0.058194014244733694 1104 72-73 -0.05636851381200003 1104 74-75 0.02435731625038784 1104 76-77 0.069595888460114 1104 78-79 0.25443025459541957 1104 80-81 0.3423835453237487 1104 82-83 0.4059776239705464 1104 84-85 0.4217446957456161 1104 86-87 0.5940739545504456 1104 88-89 0.5895744212311804 1104 90-91 0.5072441644052788 1104 92-93 0.7454457123292499 1104 94-95 0.9153625365581561 1104 96-97 1.2217491354208079 1104 98-99 1.5181563569863066 1104 100-101 2.2253836166889265 1105 1 0.18385491195157044 1105 2 0.16640877992497138 1105 3 0.23373018297805004 1105 4 0.1606723539176329 1105 5 0.08865261967822136 1105 6 0.06977906426126168 1105 7 0.02953870118705737 1105 8 0.09252540741629645 1105 9 0.09973804161254662 1105 10-11 0.14091373404993135 1105 12-13 0.16850308248776713 1105 14-15 0.1648445391405815 1105 16-17 0.2759135720530992 1105 18-19 0.16410951006619712 1105 20-21 0.19167349354000862 1105 22-23 0.2001894897501728 1105 24-25 0.1980722455298931 1105 26-27 0.10647775729250242 1105 28-29 0.11086701396835963 1105 30-31 0.17317001071886295 1105 32-33 0.15701996830059528 1105 34-35 0.2882676295483364 1105 36-37 0.36784078629769823 1105 38-39 0.3351437146192424 1105 40-41 0.3742926931586297 1105 42-43 0.15603515812222923 1105 44-45 0.21066357477995723 1105 46-47 0.4338383438116864 1105 48-49 0.4703217873253749 1105 50-51 0.49973626658876213 1105 52-53 0.4363835054699692 1105 54-55 0.48246655435372787 1105 56-57 0.6723856155247319 1105 58-59 0.7416032088584217 1105 60-61 0.7083061835860818 1105 62-63 0.6209761022793288 1105 64-65 0.609522271727954 1105 66-67 0.7008772581486014 1105 68-69 0.8673164374740523 1105 70-71 0.8312260365062514 1105 72-73 0.828578647149687 1105 74-75 0.7059345575910108 1105 76-77 0.8159351478146775 1105 78-79 1.0164642971062143 1105 80-81 1.1700519714505724 1105 82-83 1.1280336039582295 1105 84-85 1.2027608939124264 1105 86-87 1.3388237865212425 1105 88-89 1.4296450595602046 1105 90-91 1.3097384260699094 1105 92-93 1.643043636586473 1105 94-95 1.8374974897298308 1105 96-97 1.9576857463362174 1105 98-99 2.1312895584954994 1105 100-101 2.4263663628027032 1106 1 0.07052863114833485 1106 2 0.06242856035454025 1106 3 0.14426225302484852 1106 4 0.048763248192621234 1106 5 0.04865243627909166 1106 6 9.09565661970646E-4 1106 7 0.023832070600001032 1106 8 -0.052837783295032636 1106 9 -0.017332499110608524 1106 10-11 0.06436095824160759 1106 12-13 0.04582271673989169 1106 14-15 0.0030675433099958127 1106 16-17 0.069793063820768 1106 18-19 0.07431688029359407 1106 20-21 0.0222392715128521 1106 22-23 0.027688334127255132 1106 24-25 0.10458078880312627 1106 26-27 0.01680673364610641 1106 28-29 -0.040746335654148425 1106 30-31 -0.0037052141013731443 1106 32-33 0.0032340684423530774 1106 34-35 -0.07855302986322243 1106 36-37 0.05549998403488843 1106 38-39 0.16299330982819527 1106 40-41 0.1684428028438063 1106 42-43 0.09419505631904457 1106 44-45 0.29306938786540826 1106 46-47 -0.0508926367609277 1106 48-49 0.12619562373085813 1106 50-51 0.1529040280231797 1106 52-53 0.07920672246573446 1106 54-55 0.08512734224471785 1106 56-57 0.1755562321501074 1106 58-59 0.24880943992715743 1106 60-61 0.30155942985484074 1106 62-63 0.1240671070106103 1106 64-65 0.23965062194025677 1106 66-67 0.3320269122536743 1106 68-69 -0.030661441256611965 1106 70-71 0.14840742949764518 1106 72-73 0.18561016970840782 1106 74-75 0.43461545102820054 1106 76-77 0.6127473583618794 1106 78-79 0.6686771326276251 1106 80-81 0.8761550776065725 1106 82-83 0.7737469202771265 1106 84-85 0.8895326899609266 1106 86-87 0.9737741791412624 1106 88-89 1.1374709712727018 1106 90-91 1.1447637205609702 1106 92-93 1.2671878608269225 1106 94-95 1.494309058380086 1106 96-97 1.7317252038249293 1106 98-99 1.8757077643601896 1106 100-101 2.1295606129065945 1107 1 0.008914091091376974 1107 2 0.058906888215716435 1107 3 0.12991459529379057 1107 4 0.06537613450363722 1107 5 0.07654709669647985 1107 6 0.047700895193031556 1107 7 -0.014202472522434562 1107 8 0.04956337513382891 1107 9 0.1119218631484884 1107 10-11 0.05792728152070481 1107 12-13 0.03865271972470197 1107 14-15 0.08386936807746537 1107 16-17 0.0900619387088426 1107 18-19 0.05172852589181076 1107 20-21 0.06892230085272644 1107 22-23 0.00912564102217317 1107 24-25 0.0707790285720904 1107 26-27 0.07747077314239448 1107 28-29 0.08572812002142172 1107 30-31 0.008967118325756473 1107 32-33 0.10975970957141357 1107 34-35 0.07365129246395696 1107 36-37 0.09318038351536728 1107 38-39 0.11359168351023641 1107 40-41 0.15578257099669912 1107 42-43 0.13081806678201957 1107 44-45 0.05906937979835192 1107 46-47 0.15792283746080926 1107 48-49 0.26650164739958626 1107 50-51 0.22114303061270135 1107 52-53 0.01737921220040306 1107 54-55 0.08255233881622104 1107 56-57 0.24724732854333098 1107 58-59 0.14591195604108975 1107 60-61 0.27822567972375367 1107 62-63 0.37493068507144756 1107 64-65 0.39559160337797294 1107 66-67 0.5649538005491905 1107 68-69 0.5575229473817487 1107 70-71 0.7019849659808983 1107 72-73 0.6512324218683609 1107 74-75 0.6640249023879008 1107 76-77 0.6434975539879204 1107 78-79 0.9255801101770125 1107 80-81 1.1446338733568489 1107 82-83 1.2203367751508338 1107 84-85 1.3263224867518524 1107 86-87 1.558951775320704 1107 88-89 1.5531162145346933 1107 90-91 1.641490715341586 1107 92-93 1.6773475078126658 1107 94-95 1.7607436628121214 1107 96-97 1.8485016496425644 1107 98-99 2.022630183508145 1107 100-101 2.4548744212818114 1108 1 0.07772370508538984 1108 2 0.059021477168499814 1108 3 0.0931043767904427 1108 4 -0.0038388450846014166 1108 5 -0.05042530630463915 1108 6 -0.0023325823808164614 1108 7 0.01424849144039797 1108 8 0.03775535658533613 1108 9 -0.01930640034105835 1108 10-11 0.04829712262657182 1108 12-13 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2316 100-101 -3.8089077627862693 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 2.0 3 8.0 4 15.0 5 31.0 6 90.0 7 220.0 8 493.0 9 957.0 10 1690.0 11 2639.0 12 4111.0 13 6209.0 14 8516.0 15 11725.0 16 15538.0 17 21623.0 18 21863.0 19 16401.0 20 14538.0 21 14694.0 22 15506.0 23 16798.0 24 19058.0 25 22255.0 26 25778.0 27 31349.0 28 37528.0 29 45493.0 30 55521.0 31 68669.0 32 85843.0 33 112298.0 34 154663.0 35 228044.0 36 343790.0 37 460821.0 38 276679.0 39 16554.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.39329132242607 18.73910744662562 15.228745358573242 22.63885587237507 2 16.053158314027236 20.021308928014474 39.3450957256552 24.58043703230309 3 19.89644504256013 23.599510261885694 29.601165584126704 26.90287911142747 4 13.339969073373585 16.892870332913038 36.03540482416447 33.731755769548904 5 13.892553539032578 36.97438143099761 33.910811142698925 15.222253887270881 6 32.626971380061036 35.69971538540672 16.645412478000523 15.027900756531723 7 29.006617667843216 32.81498565113895 19.73456113059665 18.4438355504212 8 26.99933132808992 34.12673441120959 18.982450953633652 19.89148330706684 9 26.300839059671148 16.855011397068875 19.948508140134596 36.89564140312538 10-11 25.457886915312294 26.381885912537058 26.175538493547997 21.984688678602655 12-13 26.47641740473056 23.625389305244504 26.99050190198289 22.907691388042046 14-15 23.057783301382614 25.08835682487253 25.840994323013184 26.012865550731668 16-17 22.254543651538384 28.27728866998361 26.612677136492817 22.855490541985187 18-19 22.231675371441572 26.56075432423653 29.15687176756745 22.050698536754446 20-21 22.83876217498428 25.468243674383494 29.626609873629008 22.066384277003223 22-23 22.658665780665622 25.278670961362103 29.657656981359224 22.405006276613047 24-25 22.599092853673998 25.593310583620948 29.62535264525648 22.18224391744857 26-27 22.024466273302277 25.89373136043965 29.665084078336385 22.416718287921693 28-29 22.077821877150424 26.382770197474052 29.437373870774163 22.102034054601358 30-31 22.217158400563484 25.77355990009314 29.357185555210584 22.652096144132788 32-33 22.037651089890826 25.81249155736324 29.3941621787946 22.755695173951334 34-35 22.780679441091678 26.0159278258659 29.07100504919542 22.132387683847004 36-37 22.78288719547305 25.595684006349884 29.265821822757953 22.355606975419107 38-39 22.244997324537017 26.01069119178261 29.173299319767303 22.571012163913068 40-41 22.665193990889556 25.851091343595716 29.09983851859817 22.38387614691656 42-43 22.385801835438958 25.846333316493876 28.772204329656 22.995660518411164 44-45 22.44806502493143 25.989929180790693 29.002694156158178 22.559311638119702 46-47 22.94700726216429 26.382303146180973 28.28546146822088 22.385228123433855 48-49 22.7729335447012 26.30663385243885 28.219533115508895 22.700899487351062 50-51 22.684291229284746 26.641106092601014 28.27358155264269 22.40102112547156 52-53 23.03206517482997 26.564160238293503 27.80238376912815 22.601390817748378 54-55 22.592401058196646 26.651787224439545 27.978634029205594 22.777177688158215 56-57 22.80287264522748 26.729219637898044 27.82295827037026 22.64494944650421 58-59 22.914781249956555 26.31397615674804 27.833036995640892 22.938205597654505 60-61 22.50551549551879 26.663209779746254 28.219851520682703 22.61142320405225 62-63 22.42110906756268 26.683900128405273 28.334795327734657 22.560195476297388 64-65 22.157115973922284 26.778223095248354 28.113341405581345 22.951319525248017 66-67 22.35318404751048 26.75854306378846 27.809558629995813 23.07871425870525 68-69 22.450809982720184 26.413930618454323 28.144877987070487 22.990381411755003 70-71 22.546224277818787 26.55005002291462 27.77154518674835 23.13218051251824 72-73 22.478893759114296 26.60243622483852 27.829399386750115 23.089270629297072 74-75 22.355608938045265 27.2063024701913 27.37882244344445 23.059266148318986 76-77 22.45530722503268 26.692102125522066 27.7212674078121 23.131323241633154 78-79 22.549143632724764 26.761795931531395 27.756206988750286 22.93285344699355 80-81 22.69759150401076 26.810623600994717 27.489444540598 23.002340354396523 82-83 22.57714579108727 26.82971021208605 27.67276245695072 22.920381539875958 84-85 22.51584506797496 26.933696088885306 27.537724862796566 23.01273398034317 86-87 22.599825903170746 26.976273572523006 27.350950153024023 23.072950371282225 88-89 22.45621083488453 27.05806411552119 27.12414348212312 23.361581567471156 90-91 22.50875433256338 26.851385227010216 27.321210149627085 23.318650290799315 92-93 22.588753040146266 26.60578921235507 27.44304728946502 23.362410458033644 94-95 22.315909467413807 26.570667618161657 27.544274058361708 23.569148856062828 96-97 22.410294503351704 26.391770921860275 27.60715215805702 23.590782416731003 98-99 22.25014150791483 26.617760419164632 27.16822215317702 23.963875919743522 100-101 22.492308676146955 26.095194005795314 27.356622091975215 24.05587522608252 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1199.0 1 822.5 2 578.5 3 767.0 4 635.5 5 559.5 6 1187.5 7 2678.0 8 4379.5 9 5069.5 10 4083.0 11 2804.0 12 2550.5 13 2692.5 14 2838.0 15 2946.5 16 3025.5 17 3194.5 18 3447.5 19 3722.5 20 4083.5 21 4786.0 22 5676.5 23 6670.5 24 7875.0 25 9671.5 26 12272.0 27 15419.5 28 18006.5 29 20172.5 30 23080.5 31 26957.0 32 32157.5 33 34840.0 34 35813.5 35 38385.0 36 42243.5 37 46464.0 38 49289.0 39 51530.5 40 54572.5 41 58725.5 42 61325.0 43 63622.0 44 67567.0 45 71771.5 46 74314.5 47 79185.0 48 85391.0 49 84728.5 50 80731.5 51 78142.5 52 77656.5 53 79517.5 54 82331.0 55 78633.5 56 70827.5 57 65336.0 58 60979.0 59 54386.5 60 44404.5 61 36400.0 62 29870.5 63 22622.5 64 16330.0 65 11955.5 66 8705.5 67 6695.5 68 5062.0 69 3618.5 70 2733.0 71 1868.5 72 1276.5 73 950.0 74 687.0 75 467.0 76 289.5 77 150.5 78 84.0 79 48.0 80 25.0 81 15.5 82 12.0 83 6.0 84 2.0 85 2.5 86 3.0 87 3.0 88 2.5 89 0.5 90 0.5 91 0.5 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.010657962355150182 3 0.005699692911667271 4 0.0 5 2.780338005691352E-4 6 2.3169483380761267E-4 7 0.0 8 9.267793352304506E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016218638366532886 26-27 0.021756144894534826 28-29 2.780338005691352E-4 30-31 4.633896676152253E-5 32-33 0.003035202322879726 34-35 0.015778418182298422 36-37 0.008178827633408726 38-39 0.020412314858450673 40-41 0.02256707681286147 42-43 0.01760880736937856 44-45 0.008920251101593087 46-47 0.0021084229876492753 48-49 1.8535586704609012E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.390169002845676E-4 68-69 9.267793352304506E-5 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.0852535042685137E-4 82-83 8.804403684689281E-4 84-85 0.0038924732079678925 86-87 7.645929515651218E-4 88-89 0.0 90-91 0.003683947857541041 92-93 6.719150180420767E-4 94-95 1.6218638366532885E-4 96-97 2.3169483380761267E-4 98-99 2.0852535042685137E-4 100-101 0.002780338005691352 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2158011.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 51.803778236997076 #Duplication Level Percentage of deduplicated Percentage of total 1 75.65057150487544 39.189854297406576 2 13.629105820285679 14.120783509652906 3 4.404762879206468 6.845500781429058 4 1.937535327354945 4.014866016985724 5 1.059968116200523 2.745517661496972 6 0.6706865309098863 2.084645778827798 7 0.4517225293010168 1.63806536127957 8 0.3362805264343497 1.393648145346055 9 0.24344042631151688 1.1350020472705664 >10 1.4549674906277692 14.064787625871173 >50 0.10528950040792787 3.710834453868554 >100 0.049138945928880594 4.579447939001753 >500 0.00429396306120182 1.60112984931902 >1k 0.001968066403050825 1.5991834686049367 >5k 8.945756377503751E-5 0.2816690947658747 >10k+ 1.7891512755007503E-4 0.9950639688734555 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11432 0.5297470680177256 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10040 0.4652432262856862 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6078 0.281648239976534 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3412 0.15810855459031486 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2614 0.12113005911461991 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 2.3169483380761265E-5 0.0 12-13 0.0 0.0 0.0 4.633896676152253E-5 0.0 14-15 0.0 0.0 0.0 1.1584741690380632E-4 4.633896676152253E-5 16-17 0.0 0.0 0.0 1.390169002845676E-4 9.267793352304506E-5 18-19 0.0 0.0 0.0 1.390169002845676E-4 9.267793352304506E-5 20-21 0.0 0.0 0.0 2.780338005691352E-4 9.267793352304506E-5 22-23 0.0 0.0 0.0 5.328981177575091E-4 9.267793352304506E-5 24-25 0.0 0.0 0.0 8.109319183266443E-4 9.267793352304506E-5 26-27 0.0 0.0 0.0 0.0014133384862264372 1.390169002845676E-4 28-29 0.0 0.0 0.0 0.0033364056068296223 1.6218638366532887E-4 30-31 0.0 0.0 0.0 0.009267793352304507 1.8535586704609012E-4 32-33 0.0 0.0 0.0 0.023818228915422582 2.3169483380761267E-4 34-35 0.0 0.0 0.0 0.04543535690967285 2.3169483380761267E-4 36-37 4.633896676152253E-5 0.0 0.0 0.08271505566931772 2.3169483380761267E-4 38-39 4.633896676152253E-5 0.0 0.0 0.13669995194649148 2.3169483380761267E-4 40-41 4.633896676152253E-5 0.0 0.0 0.19212135619327242 2.3169483380761267E-4 42-43 6.95084501422838E-5 0.0 0.0 0.25076331862997914 2.3169483380761267E-4 44-45 9.267793352304506E-5 0.0 0.0 0.3193681589204133 2.3169483380761267E-4 46-47 9.267793352304506E-5 0.0 0.0 0.4017820113057811 2.3169483380761267E-4 48-49 9.267793352304506E-5 0.0 0.0 0.48479827025904876 2.3169483380761267E-4 50-51 9.267793352304506E-5 0.0 0.0 0.5739081033414566 2.3169483380761267E-4 52-53 9.267793352304506E-5 0.0 0.0 0.6608863439528343 2.3169483380761267E-4 54-55 9.267793352304506E-5 0.0 0.0 0.757387242233705 2.3169483380761267E-4 56-57 9.267793352304506E-5 0.0 0.0 0.8694116943796857 2.3169483380761267E-4 58-59 9.267793352304506E-5 0.0 0.0 0.9879467713556603 2.3169483380761267E-4 60-61 9.267793352304506E-5 0.0 0.0 1.1159349975509856 2.3169483380761267E-4 62-63 9.267793352304506E-5 0.0 0.0 1.2369723787320823 2.3169483380761267E-4 64-65 9.267793352304506E-5 0.0 0.0 1.3653776556282615 2.3169483380761267E-4 66-67 9.267793352304506E-5 0.0 0.0 1.512063654911861 2.3169483380761267E-4 68-69 9.267793352304506E-5 0.0 0.0 1.6742732080605705 2.780338005691352E-4 70-71 9.267793352304506E-5 0.0 0.0 1.8444530634922622 2.780338005691352E-4 72-73 9.267793352304506E-5 0.0 0.0 2.012779360253493 3.2437276733065774E-4 74-75 9.267793352304506E-5 0.0 0.0 2.186365129742156 3.2437276733065774E-4 76-77 9.267793352304506E-5 0.0 0.0 2.3716283188547234 3.2437276733065774E-4 78-79 9.267793352304506E-5 0.0 0.0 2.585436311492388 3.2437276733065774E-4 80-81 9.267793352304506E-5 0.0 0.0 2.807562148663746 3.47542250711419E-4 82-83 9.267793352304506E-5 0.0 0.0 3.0307537820706196 3.7071173409218024E-4 84-85 1.1584741690380632E-4 0.0 0.0 3.2553355844803384 3.938812174729415E-4 86-87 1.390169002845676E-4 0.0 0.0 3.4919655182480533 4.170507008537028E-4 88-89 1.390169002845676E-4 0.0 0.0 3.7460420730014814 4.170507008537028E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8820 0.0 32.853004 1 GTATCAA 12845 0.0 30.731287 1 ATCAACG 15470 0.0 25.36082 3 TATCAAC 15585 0.0 25.236979 2 TCAACGC 15675 0.0 25.119473 4 CAACGCA 15820 0.0 24.829193 5 AACGCAG 16215 0.0 24.312223 6 TAAGGTG 1195 0.0 24.245182 5 GTAAGGT 1115 0.0 23.854849 4 GTACATG 8520 0.0 22.970554 1 GAGTACT 9625 0.0 22.87244 12-13 TACATGG 8475 0.0 22.532022 2 GTACTTT 9865 0.0 22.027111 14-15 GGACCGA 670 0.0 21.976088 6 ACGCAGA 17915 0.0 21.925636 7 CGCAGAG 17820 0.0 21.829294 8 AGTACTT 9780 0.0 21.490076 12-13 AGAGTAC 13830 0.0 21.361498 10-11 ACATGGG 8690 0.0 21.316624 3 GCAGAGT 18925 0.0 20.353941 9 >>END_MODULE