FastQCFastQC Report
Thu 26 May 2016
SRR938125_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938125_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152609
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT120010.5575095151976044No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98590.4580023589978487No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60480.28096138221107503No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34540.160456450753481No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25460.11827507921782357No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA82000.036.695661
GTATCAA122700.032.97061
TATCAAC148500.027.104882
ATCAACG149200.026.945863
TCAACGC149800.026.93314
CAACGCA151800.026.5469465
AACGCAG154850.026.177516
TAACACG950.001223664325.0113374
GAGTACT94300.023.41808912-13
ACGCAGA172200.023.4156287
CGCAGAG171900.023.401178
TAAGGTG11400.023.3439145
GTAAGGT10450.022.7375774
GTACATG84500.022.3268971
GTACTTT98950.022.0374414-15
TACATGG83250.021.9198442
GCAGAGT180150.021.9056729
TATTCCG1754.0360646E-621.724135
AGAGTAC139400.021.6241610-11
AGTACTT98150.020.90104112-13
Produced by FastQC (version 0.11.5)