##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938125_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2152609 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.823719031184947 33.0 31.0 34.0 30.0 34.0 2 32.014308218538524 34.0 31.0 34.0 30.0 34.0 3 32.07698239671022 34.0 31.0 34.0 30.0 34.0 4 35.5983752739118 37.0 35.0 37.0 33.0 37.0 5 35.61678688512405 37.0 35.0 37.0 33.0 37.0 6 35.64633289185356 37.0 35.0 37.0 33.0 37.0 7 35.58589181778948 37.0 35.0 37.0 33.0 37.0 8 35.56629373936465 37.0 35.0 37.0 33.0 37.0 9 37.21131612847479 39.0 37.0 39.0 34.0 39.0 10-11 37.12959994128056 39.0 37.0 39.0 34.0 39.0 12-13 37.02556827552054 39.0 37.0 39.0 33.0 39.0 14-15 38.246992138377195 40.0 38.0 41.0 33.0 41.0 16-17 38.167073537275 40.0 38.0 41.0 33.0 41.0 18-19 38.12149744798057 40.0 38.0 41.0 33.0 41.0 20-21 38.015561581318295 40.0 38.0 41.0 33.0 41.0 22-23 37.86193869857461 40.0 38.0 41.0 33.0 41.0 24-25 37.806431637143575 40.0 38.0 41.0 32.5 41.0 26-27 37.7991783459049 40.0 38.0 41.0 33.0 41.0 28-29 37.70828492308635 40.0 38.0 41.0 32.0 41.0 30-31 37.50938349695649 40.0 37.0 41.0 32.0 41.0 32-33 37.26533220849676 40.0 37.0 41.0 31.5 41.0 34-35 37.043618232572655 40.0 36.0 41.0 31.0 41.0 36-37 36.81813046400902 40.0 36.0 41.0 30.0 41.0 38-39 36.56571421005859 39.0 35.5 41.0 30.0 41.0 40-41 36.33366579810824 39.0 35.0 41.0 30.0 41.0 42-43 36.03524490513605 39.0 35.0 41.0 29.0 41.0 44-45 35.78392499520349 39.0 35.0 40.5 28.0 41.0 46-47 35.60278364533457 39.0 35.0 41.0 27.0 41.0 48-49 35.3149338779128 39.0 35.0 41.0 26.0 41.0 50-51 34.65973592974851 38.5 34.0 40.0 24.5 40.5 52-53 34.730021336898616 38.5 34.5 40.0 24.5 41.0 54-55 34.865050271554196 39.0 35.0 40.0 24.0 41.0 56-57 34.64011508824872 38.0 34.0 40.0 24.0 41.0 58-59 34.36582096423456 38.0 34.0 40.0 23.0 41.0 60-61 33.97163349219482 37.5 34.0 40.0 22.0 41.0 62-63 33.558668109257184 37.0 33.5 40.0 21.5 41.0 64-65 33.11596067841396 36.5 33.0 39.5 19.5 41.0 66-67 32.669317093814996 36.0 32.5 39.0 17.5 41.0 68-69 32.16763703951809 35.0 32.0 39.0 13.5 40.0 70-71 31.671710700828623 35.0 31.0 38.0 9.0 40.0 72-73 31.17766347720371 35.0 31.0 37.0 7.0 39.5 74-75 30.658281415714605 35.0 30.5 37.0 3.5 39.0 76-77 30.117645378236364 34.5 30.0 36.0 2.0 39.0 78-79 29.544024948330144 34.0 29.0 36.0 2.0 37.5 80-81 29.02060801566843 34.0 29.0 35.0 2.0 37.0 82-83 28.482317968567443 34.0 28.5 35.0 2.0 36.5 84-85 27.98974337652588 34.0 27.0 35.0 2.0 36.0 86-87 27.488890690320442 33.5 26.0 35.0 2.0 36.0 88-89 26.934051423179966 33.0 25.5 35.0 2.0 35.0 90-91 26.31848422077581 32.5 24.5 35.0 2.0 35.0 92-93 25.697595104359408 32.0 21.5 35.0 2.0 35.0 94-95 25.08545211880095 32.0 19.0 34.0 2.0 35.0 96-97 24.242411417958394 31.5 8.5 34.0 2.0 35.0 98-99 23.17470590339444 31.0 2.0 34.0 2.0 35.0 100-101 20.40567167562711 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4321766381070482 1101 2 0.297110922431429 1101 3 0.281209445167903 1101 4 0.191911945595038 1101 5 0.16062100316896277 1101 6 0.24849283381735177 1101 7 0.2523924916684166 1101 8 0.1913352722149071 1101 9 0.23040387163558762 1101 10-11 0.3521532986211753 1101 12-13 0.15801005247754318 1101 14-15 0.2128380397186831 1101 16-17 0.4373736878853265 1101 18-19 0.4202358890412512 1101 20-21 0.45746573943001323 1101 22-23 0.4107452710149673 1101 24-25 0.5579213230734297 1101 26-27 0.46355916189871493 1101 28-29 0.4546708040539045 1101 30-31 0.5460169450197796 1101 32-33 0.4045007967144585 1101 34-35 0.5849514135965777 1101 36-37 0.5590854179411622 1101 38-39 0.4681305207689519 1101 40-41 0.5040409766514884 1101 42-43 0.5642288699011004 1101 44-45 0.6064172562202188 1101 46-47 0.6531865395602381 1101 48-49 0.6836031887986209 1101 50-51 0.6358909236269099 1101 52-53 0.7269581059851546 1101 54-55 0.6587711559933211 1101 56-57 0.475804271974944 1101 58-59 0.3914000506622273 1101 60-61 0.4784627604357894 1101 62-63 0.5076119750117982 1101 64-65 0.4692753686550972 1101 66-67 0.4688399684445912 1101 68-69 0.5818818154923449 1101 70-71 0.5416958680292332 1101 72-73 0.4902369875709951 1101 74-75 0.46930016000761654 1101 76-77 0.48817129960801964 1101 78-79 0.5751419294171995 1101 80-81 0.48749483372387203 1101 82-83 0.5434884632631345 1101 84-85 0.5528882873096315 1101 86-87 0.5918412666285384 1101 88-89 0.6908821129640863 1101 90-91 0.7223159260661198 1101 92-93 0.8533422754328832 1101 94-95 0.9857759312912897 1101 96-97 0.8782036855242161 1101 98-99 0.8296288738889608 1101 100-101 1.241055067480623 1102 1 0.3655657594459605 1102 2 0.2822199879823373 1102 3 0.23518908254587245 1102 4 0.11613983959782104 1102 5 0.08406089159294794 1102 6 0.21576982126504873 1102 7 0.09513726154289515 1102 8 0.1455743238188134 1102 9 0.25810791626599183 1102 10-11 0.18000476305631707 1102 12-13 0.14071005247754442 1102 14-15 0.27495951810083596 1102 16-17 0.26809697937765975 1102 18-19 0.20186461986411786 1102 20-21 0.30284328475777755 1102 22-23 0.4304590785463489 1102 24-25 0.2753066787219609 1102 26-27 0.20609695827249652 1102 28-29 0.19520218480704443 1102 30-31 0.2581848669165794 1102 32-33 0.48909535738391696 1102 34-35 0.5428323062046658 1102 36-37 0.49491805392442956 1102 38-39 0.4416309391789923 1102 40-41 0.5926327479206677 1102 42-43 0.7078892604171401 1102 44-45 0.5536048433889746 1102 46-47 0.7254063443022147 1102 48-49 0.6798666476549684 1102 50-51 0.758404452218258 1102 52-53 0.813451411424488 1102 54-55 0.6763406120602724 1102 56-57 0.6258338396179042 1102 58-59 0.5868220869244283 1102 60-61 0.5518859124581041 1102 62-63 0.5678397295445734 1102 64-65 0.46444663783222495 1102 66-67 0.5122352264641137 1102 68-69 0.5990701000111684 1102 70-71 0.7069791316275662 1102 72-73 0.6589316877104672 1102 74-75 0.5751489745124978 1102 76-77 0.6545432661352173 1102 78-79 0.6087995305329592 1102 80-81 0.4906383483682255 1102 82-83 0.5161906947833579 1102 84-85 0.5779193891227443 1102 86-87 0.5423638607707986 1102 88-89 0.7075935495052299 1102 90-91 0.8775892872934534 1102 92-93 0.9778612433547806 1102 94-95 1.1244748155311761 1102 96-97 0.9967495711588086 1102 98-99 1.0858574652418191 1102 100-101 1.2820197815670973 1103 1 0.25177218916872235 1103 2 0.2669198877162273 1103 3 0.21854628372537377 1103 4 0.05734720006082483 1103 5 -0.00813315928469649 1103 6 0.0318893960013753 1103 7 0.0763722692215012 1103 8 0.1679157912577125 1103 9 0.23980353459480597 1103 10-11 0.12418969902562083 1103 12-13 0.028285886653478087 1103 14-15 0.0934088519869718 1103 16-17 0.33703786045020934 1103 18-19 0.26855162884576345 1103 20-21 0.1926100602928429 1103 22-23 0.27905831449323415 1103 24-25 0.30116459910982485 1103 26-27 0.08445555556235007 1103 28-29 0.2343593783713942 1103 30-31 0.19678351057421395 1103 32-33 0.21032041248797384 1103 34-35 0.3444645918911462 1103 36-37 0.2270975514093081 1103 38-39 0.26524032865570746 1103 40-41 0.2499064299713467 1103 42-43 0.16498451533420422 1103 44-45 0.20987057607191417 1103 46-47 0.35880891906816004 1103 48-49 0.3429251975616836 1103 50-51 0.31496648817021367 1103 52-53 0.3459514325776709 1103 54-55 0.2864876372875642 1103 56-57 0.09087714019199922 1103 58-59 0.2625384396072903 1103 60-61 0.29868331992349084 1103 62-63 0.332189258463103 1103 64-65 0.44199623147950007 1103 66-67 0.4675977709386885 1103 68-69 0.5038818154923419 1103 70-71 0.7342289317299482 1103 72-73 0.8658492558554656 1103 74-75 0.5849703993065738 1103 76-77 0.6147136656343122 1103 78-79 0.7820243021843041 1103 80-81 0.7757544899422761 1103 82-83 0.7999731279075561 1103 84-85 0.85151814912291 1103 86-87 0.8472127529783862 1103 88-89 0.8535783044032463 1103 90-91 0.9450007929350086 1103 92-93 0.8509889218771036 1103 94-95 0.9836895140348005 1103 96-97 0.7837724081396544 1103 98-99 0.9955299187153841 1103 100-101 0.9471249023306392 1104 1 0.283324179090652 1104 2 0.33227183532117976 1104 3 0.23914168560506255 1104 4 0.2074925306159301 1104 5 0.2160537257661943 1104 6 0.24052137769391635 1104 7 0.1745988561814329 1104 8 0.25582103242062715 1104 9 0.21806391663719182 1104 10-11 0.2563018039249272 1104 12-13 0.23430937745343527 1104 14-15 0.2473056707055079 1104 16-17 0.4573604445552064 1104 18-19 0.46166063992521345 1104 20-21 0.31535246395589667 1104 22-23 0.391066196762317 1104 24-25 0.32314870976581034 1104 26-27 0.3938377861353004 1104 28-29 0.5876466960500508 1104 30-31 0.5619588286584829 1104 32-33 0.5292788102149473 1104 34-35 0.5026803753452072 1104 36-37 0.6531664208341255 1104 38-39 0.5141602218297052 1104 40-41 0.43022625469713915 1104 42-43 0.5544365201743275 1104 44-45 0.4511032092899683 1104 46-47 0.5172665138450299 1104 48-49 0.6087614015919343 1104 50-51 0.5064430290592412 1104 52-53 0.4923633133139873 1104 54-55 0.5423225542575523 1104 56-57 0.6156691385773243 1104 58-59 0.5707927861170674 1104 60-61 0.5705570066588663 1104 62-63 0.6126959994412431 1104 64-65 0.6318444461221517 1104 66-67 0.5344186248251717 1104 68-69 0.6494941587903185 1104 70-71 0.6068565237669468 1104 72-73 0.7956959396764276 1104 74-75 0.8458828665328504 1104 76-77 0.71401816556752 1104 78-79 0.9096999493464821 1104 80-81 1.095460503926379 1104 82-83 1.0632313112219904 1104 84-85 1.0009848479010799 1104 86-87 1.0185521634462802 1104 88-89 0.9887788664195654 1104 90-91 1.20259493603076 1104 92-93 1.3984695014052413 1104 94-95 1.3065656452096412 1104 96-97 1.3460851384332493 1104 98-99 1.3566546533810815 1104 100-101 1.193087725789848 1105 1 0.5513120847929294 1105 2 0.4941460809328717 1105 3 0.5005785133715577 1105 4 0.35898898690147263 1105 5 0.3914636544657881 1105 6 0.4210238616751809 1105 7 0.34932236678849193 1105 8 0.3443527554041452 1105 9 0.5347865805693033 1105 10-11 0.5830236156240503 1105 12-13 0.4260726845620795 1105 14-15 0.6847619590270497 1105 16-17 0.6599700375491153 1105 18-19 0.6485590398577727 1105 20-21 0.5625220314569148 1105 22-23 0.5969800452704916 1105 24-25 0.6392704104893738 1105 26-27 0.6097943204001623 1105 28-29 0.7559465004996255 1105 30-31 0.7157299133194996 1105 32-33 0.6809718822091781 1105 34-35 0.7191769659500764 1105 36-37 0.809196849257205 1105 38-39 0.859475765725719 1105 40-41 0.8946503906764676 1105 42-43 0.7692874674035011 1105 44-45 1.0261175444046486 1105 46-47 0.9308683839406413 1105 48-49 0.847246992833206 1105 50-51 0.7771258900053866 1105 52-53 0.8435321693857318 1105 54-55 0.8269463721316512 1105 56-57 0.8425358762016515 1105 58-59 0.8257850650714502 1105 60-61 0.8347367277748887 1105 62-63 0.8099159135324498 1105 64-65 0.8352354070796864 1105 66-67 1.051876663929697 1105 68-69 0.9983582804299047 1105 70-71 1.1345765596718955 1105 72-73 1.1353828088966438 1105 74-75 1.0016659621209705 1105 76-77 1.0241536242958205 1105 78-79 1.1480141676496665 1105 80-81 1.263082922100434 1105 82-83 1.2435557062986788 1105 84-85 1.3923411211232697 1105 86-87 1.6055642253221052 1105 88-89 1.4578761571523664 1105 90-91 1.3521314880257727 1105 92-93 1.2150087499765938 1105 94-95 1.6089851531933057 1105 96-97 1.5723201120371861 1105 98-99 1.6017837249936662 1105 100-101 1.3913351827543998 1106 1 0.10883869435416571 1106 2 0.1237141497251173 1106 3 0.08196039030391233 1106 4 0.05618340709803249 1106 5 0.10046646190570385 1106 6 0.2241881772936054 1106 7 0.1976834091419022 1106 8 0.07127109517479369 1106 9 -0.03345892227866187 1106 10-11 -0.45452074471677406 1106 12-13 -0.20769358975390162 1106 14-15 -0.14922042040118555 1106 16-17 0.057242383136596686 1106 18-19 0.10070135699883309 1106 20-21 -0.021371328343150253 1106 22-23 0.03898575049860398 1106 24-25 0.011784670238014883 1106 26-27 -0.04406315253189774 1106 28-29 -0.008191344401602407 1106 30-31 0.09765401279295105 1106 32-33 0.12989388108513822 1106 34-35 -0.10541317838130482 1106 36-37 -0.2217610550878817 1106 38-39 -0.11055384990878281 1106 40-41 0.20085499232691717 1106 42-43 0.18144602066181648 1106 44-45 0.32060443925387005 1106 46-47 0.5671059494265336 1106 48-49 0.43947631005265464 1106 50-51 0.6173157276840726 1106 52-53 0.5981256486315303 1106 54-55 0.12348005410305518 1106 56-57 0.33760159793160227 1106 58-59 0.3864868187581294 1106 60-61 0.2689841066782961 1106 62-63 0.4135619980638978 1106 64-65 0.2972834829934996 1106 66-67 0.05606025429059258 1106 68-69 -0.26548194661000935 1106 70-71 0.16651799804307288 1106 72-73 0.3836492512869967 1106 74-75 0.4220319257983043 1106 76-77 0.41587918342544583 1106 78-79 0.38484225214656576 1106 80-81 0.2741452717137314 1106 82-83 0.30567380212897177 1106 84-85 0.22077837490759933 1106 86-87 0.5916339821656535 1106 88-89 0.4723976500779621 1106 90-91 0.26468199337824316 1106 92-93 0.41744702416501767 1106 94-95 0.3188728423101921 1106 96-97 0.3942649118958208 1106 98-99 0.29810688218771375 1106 100-101 0.1381294796521324 1107 1 0.23198047225773522 1107 2 0.10716864274648685 1107 3 0.11618355722021789 1107 4 0.12853801187531388 1107 5 0.18146760741922208 1107 6 0.1585019287794509 1107 7 0.05131892947492389 1107 8 0.07657420816378391 1107 9 0.08311090091185491 1107 10-11 0.10116266108215655 1107 12-13 0.006263998086105005 1107 14-15 0.10661768008129968 1107 16-17 0.13562650405414445 1107 18-19 0.31741728300766425 1107 20-21 0.2522165790792954 1107 22-23 0.3073298452459099 1107 24-25 0.25128342739799336 1107 26-27 0.29054611389752694 1107 28-29 0.31527891818676324 1107 30-31 0.34531740571231495 1107 32-33 0.28741082792267036 1107 34-35 0.4008389451763037 1107 36-37 0.23495166849845361 1107 38-39 0.27352472495977054 1107 40-41 0.3737086241474046 1107 42-43 0.5170159110662027 1107 44-45 0.3481102269439518 1107 46-47 0.45633399089030036 1107 48-49 0.36313964295228374 1107 50-51 0.3508718123176422 1107 52-53 0.3795719267608959 1107 54-55 0.3962384765461593 1107 56-57 0.3282124158299027 1107 58-59 0.2733158182354174 1107 60-61 0.0758372291532865 1107 62-63 0.22799384453178817 1107 64-65 0.33440445172836064 1107 66-67 0.2620295657101579 1107 68-69 0.3232086099640341 1107 70-71 0.32291851626820645 1107 72-73 0.3604553261056971 1107 74-75 0.304449989105553 1107 76-77 0.5670410276508235 1107 78-79 0.4614311254344372 1107 80-81 0.4622008524487917 1107 82-83 0.35436377906042793 1107 84-85 0.22603698696782715 1107 86-87 0.47875557781890876 1107 88-89 0.6409542187747554 1107 90-91 0.8012982414175802 1107 92-93 0.664177644729957 1107 94-95 0.7496509795896991 1107 96-97 0.71465320254012 1107 98-99 0.9074028076086798 1107 100-101 0.8150200548487341 1108 1 0.2815674784805431 1108 2 0.1974831174810845 1108 3 0.21343627190571368 1108 4 0.24127053459755388 1108 5 0.24732359691432748 1108 6 0.18700203790805858 1108 7 0.18830000611928455 1108 8 0.16019704781887611 1108 9 0.282364861705247 1108 10-11 0.3045783764335255 1108 12-13 0.13729186661716142 1108 14-15 0.20896633377530094 1108 16-17 0.285273139493448 1108 18-19 0.41622000049819263 1108 20-21 0.355688831174497 1108 22-23 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-1.254832473976883 2316 78-79 -1.2088832278155053 2316 80-81 -1.257833468162918 2316 82-83 -1.326071285164538 2316 84-85 -1.3075544799859635 2316 86-87 -1.3282568465790092 2316 88-89 -1.4521417864069868 2316 90-91 -1.44075325632382 2316 92-93 -1.4215306805419594 2316 94-95 -1.4144177794005337 2316 96-97 -1.4597535471801848 2316 98-99 -1.6066176669915428 2316 100-101 -1.4944266935256643 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6519.0 3 1868.0 4 1922.0 5 2179.0 6 2484.0 7 3098.0 8 3719.0 9 4622.0 10 5780.0 11 7144.0 12 8672.0 13 10711.0 14 12571.0 15 15123.0 16 18554.0 17 24425.0 18 21914.0 19 17193.0 20 16943.0 21 17312.0 22 18853.0 23 20242.0 24 22226.0 25 25079.0 26 28333.0 27 32732.0 28 38037.0 29 45022.0 30 53338.0 31 65293.0 32 81171.0 33 104929.0 34 144443.0 35 211836.0 36 312220.0 37 416390.0 38 296376.0 39 33333.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.111855281697046 18.306269613843625 14.887286634528207 22.69458846993112 2 15.764732129369907 19.6869633117283 39.90220798929121 24.646096569610577 3 19.57571222058502 23.69583102860038 29.519701399573293 27.208755351241305 4 13.04289415246244 16.518290667582754 36.418819633326656 34.01999554662815 5 13.934891159815145 36.947267604586074 33.73645890481017 15.381382330788615 6 32.48076976737696 35.60769395328937 16.851915582301675 15.059620697031987 7 29.030172073143408 32.77971532201303 19.851124700264332 18.338987904579227 8 26.970297839665413 33.97165414938743 19.13326324028807 19.924784770659087 9 26.588878570439224 16.864018636421523 19.921161467004666 36.62594132613459 10-11 25.4075759118417 26.334608584396936 26.292696851482766 21.965118652278598 12-13 26.524139278123737 23.678839007920082 26.95423280300247 22.84278891095371 14-15 23.226196145255148 25.030927528370473 25.863780360746407 25.879095965627968 16-17 22.26700398248034 28.233077944821673 26.56327169998571 22.936646372712282 18-19 22.283213228721543 26.512618537743588 29.15913446587884 22.045033767656026 20-21 22.963255332621106 25.447999680149657 29.597360490858698 21.991384496370536 22-23 22.72957161816352 25.29465632532011 29.581868089725205 22.393903966791168 24-25 22.62356579105517 25.607864206482844 29.54922022135517 22.21934978110681 26-27 22.083223897526246 25.860955705808657 29.640430739336693 22.4153896573284 28-29 22.079023862794855 26.423816215618178 29.404678284824143 22.092481636762827 30-31 22.269508392733535 25.773945916645864 29.280173233346673 22.676372457273928 32-33 22.073362324830125 25.795288482748635 29.396322469928627 22.735026722492616 34-35 22.804710631882397 25.94401615211927 29.088203011646875 22.16307020435146 36-37 22.78310218215483 25.579043987330518 29.2298294845068 22.408024346007853 38-39 22.295091185865896 26.01465377999067 29.129192001883137 22.561063032260297 40-41 22.71239297420459 25.87597614380642 29.03196046741036 22.37967041457863 42-43 22.357974469168877 25.893920684044463 28.78671619711731 22.961388649669352 44-45 22.486258904587615 26.036686598941234 28.93359620578955 22.543458290681613 46-47 22.95843340699498 26.454577497268183 28.222968261672577 22.364020834064256 48-49 22.800676961424628 26.353028319645865 28.185048204690684 22.66124651423882 50-51 22.648458606681928 26.698385791170505 28.236154597507262 22.417001004640305 52-53 22.992105128645974 26.582017908672277 27.847451011370662 22.578425951311086 54-55 22.613619783220248 26.634839589860064 27.994963862296622 22.75657676462306 56-57 22.767514343831426 26.73857459663601 27.887266023328838 22.606645036203727 58-59 22.930718234483017 26.346656258313033 27.827012233300458 22.895613273903493 60-61 22.50694768597505 26.59960580406236 28.312799100175628 22.580647409786962 62-63 22.39667236482063 26.666222151801982 28.3681410156034 22.568964467773988 64-65 22.208181679205577 26.709085679528982 28.2374061440425 22.845326497222946 66-67 22.361361690185607 26.668624319719434 27.871744608051998 23.098269382042965 68-69 22.47179786695995 26.37787199204158 28.260538759071295 22.88979138192717 70-71 22.62611841383682 26.50622203384474 27.817645331057932 23.050014221260508 72-73 22.53322406004485 26.577458829073358 27.885531660479185 23.003785450402606 74-75 22.400230494249843 27.211797959707653 27.52487049220513 22.86310105383737 76-77 22.642329603735128 26.635158032227462 27.649465628707848 23.073046735329562 78-79 22.67303363216719 26.746017549035777 27.72099495645265 22.85995386234438 80-81 22.875521205526194 26.810733279373594 27.420498443004487 22.893247072095726 82-83 22.785253906749148 26.863834499089034 27.515009695140908 22.835901899020914 84-85 22.73607877001537 26.98484496834503 27.47330102669863 22.805775234940974 86-87 22.820835239150316 26.939397691369383 27.264151228290817 22.97561584118948 88-89 22.760366851728435 26.98478980013143 27.074996955915143 23.179846392224988 90-91 22.804375527111393 26.832070125191354 27.149737167648208 23.213817180049052 92-93 22.897135241189773 26.610596159440565 27.309986551257182 23.182282048112484 94-95 22.804519443661384 26.507470858735665 27.347365823061043 23.34064387454191 96-97 22.894788760246996 26.324804517800505 27.402713199280647 23.377693522671844 98-99 22.757231842786187 26.494094444279213 26.99498390238825 23.75368981054635 100-101 23.16711796625281 25.82475212647494 27.20766466307954 23.800465244192708 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 1160.0 1 790.0 2 582.0 3 784.0 4 661.5 5 592.0 6 1174.5 7 2628.5 8 4398.5 9 5158.5 10 4177.5 11 2942.5 12 2653.0 13 2774.0 14 2926.0 15 2967.0 16 3078.0 17 3217.5 18 3405.5 19 3756.5 20 4176.0 21 4837.5 22 5708.5 23 6852.5 24 8153.5 25 9873.0 26 12565.0 27 15732.5 28 18247.0 29 20364.5 30 23043.5 31 26833.0 32 31875.0 33 34523.5 34 35352.0 35 37963.0 36 42249.5 37 46491.5 38 49284.5 39 51533.0 40 54461.0 41 58424.0 42 61046.0 43 63054.5 44 66599.5 45 71103.5 46 73888.0 47 78345.5 48 84103.0 49 83312.5 50 79704.5 51 77488.0 52 77332.0 53 79074.5 54 81118.0 55 77732.0 56 70291.5 57 64685.5 58 60833.0 59 54541.0 60 44559.5 61 36691.0 62 30330.0 63 23166.5 64 16997.0 65 12817.0 66 9434.5 67 7193.0 68 5498.0 69 4000.0 70 2986.5 71 2065.0 72 1462.5 73 1061.0 74 753.5 75 558.0 76 382.0 77 219.0 78 132.0 79 88.0 80 43.5 81 28.0 82 23.5 83 15.5 84 12.0 85 9.0 86 10.0 87 7.5 88 3.0 89 3.0 90 2.0 91 0.5 92 2.0 93 2.5 94 1.5 95 1.0 96 2.0 97 2.0 98 1.5 99 1.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.49367999483417563 2 0.05128660151472004 3 0.057558060939074396 4 0.06643101464316092 5 0.08125024098663529 6 0.10912339398376573 7 0.07186627947760137 8 0.05495656665934222 9 0.052169251359629173 10-11 0.07711572329206094 12-13 0.11051705163362226 14-15 0.05614117566172026 16-17 0.06092606692622766 18-19 0.059927278943830486 20-21 0.07446777375733354 22-23 0.06382952036342875 24-25 0.08838112262840117 26-27 0.06615228311318963 28-29 0.06670974617313223 30-31 0.07070489810272093 32-33 0.07054230471023767 34-35 0.07247019779253919 36-37 0.06761562364553897 38-39 0.0407644862583033 40-41 0.04489900395287765 42-43 0.04348211867552351 44-45 0.022670164437666108 46-47 0.030312053884379374 48-49 0.029731363196939153 50-51 0.027640876722154373 52-53 0.026897592642230895 54-55 0.026154308562307417 56-57 0.025620073129862413 58-59 0.022832757830149368 60-61 0.02942940403947024 62-63 0.020904864747847845 64-65 0.023599269537570456 66-67 0.017281354858220882 68-69 0.019627345235479366 70-71 0.04185618475069091 72-73 0.03797717095859025 74-75 0.033052913929097205 76-77 0.023250855125106325 78-79 0.01644516026830697 80-81 0.017978183683149147 82-83 0.023227627497608715 84-85 0.019487979470493712 86-87 0.009639465411507618 88-89 0.0415774532207196 90-91 0.05307512883203591 92-93 0.03414461242148481 94-95 0.05340031561700244 96-97 0.04378407783299243 98-99 0.03423752293147525 100-101 0.16863257563263928 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2152609.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.684253722449725 #Duplication Level Percentage of deduplicated Percentage of total 1 77.50432344347004 41.60761764326054 2 12.540872007188783 13.464947094693802 3 4.089509806102453 6.58626846093751 4 1.7916108991127166 3.8472517631949343 5 0.978945565904149 2.627698107023274 6 0.6128423977694686 1.973999206423837 7 0.4294123632719591 1.6136877583014024 8 0.3152416937121204 1.3538812055309004 9 0.2283931762183384 1.1035005498523305 >10 1.3605614233773773 13.55615666137521 >50 0.09793800272464365 3.5967745496449064 >100 0.04401515317336889 4.22427060695134 >500 0.004338402722372897 1.6561797217735084 >1k 0.0017353610889491362 1.4877901930374169 >5k 1.7353610889491363E-4 0.7409605072212333 >10k+ 8.676805444745682E-5 0.5590159707777721 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 12001 0.5575095151976044 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9859 0.4580023589978487 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6048 0.28096138221107503 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3454 0.160456450753481 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2546 0.11827507921782357 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 4.6455254995217434E-5 14-15 0.0 0.0 0.0 0.0 4.6455254995217434E-5 16-17 0.0 0.0 0.0 0.0 4.6455254995217434E-5 18-19 0.0 0.0 0.0 0.0 4.6455254995217434E-5 20-21 0.0 0.0 0.0 2.3227627497608717E-5 4.6455254995217434E-5 22-23 0.0 0.0 0.0 6.968288249282616E-5 6.968288249282616E-5 24-25 0.0 0.0 0.0 4.180972949569569E-4 9.291050999043487E-5 26-27 0.0 0.0 0.0 0.0010220156098947836 1.393657649856523E-4 28-29 0.0 0.0 0.0 0.00325186784966522 1.62593392483261E-4 30-31 0.0 0.0 0.0 0.009616237784010008 1.8582101998086974E-4 32-33 0.0 0.0 0.0 0.024365781244991544 1.8582101998086974E-4 34-35 0.0 0.0 0.0 0.04613006821025091 2.3227627497608716E-4 36-37 0.0 0.0 0.0 0.08071600555419028 2.3227627497608716E-4 38-39 0.0 0.0 0.0 0.1324439319913649 2.3227627497608716E-4 40-41 0.0 0.0 0.0 0.1892122535955206 2.3227627497608716E-4 42-43 0.0 0.0 0.0 0.24739746047703043 2.3227627497608716E-4 44-45 9.291050999043487E-5 0.0 0.0 0.31624414837994264 2.3227627497608716E-4 46-47 9.291050999043487E-5 0.0 0.0 0.39837703921148704 2.3227627497608716E-4 48-49 9.291050999043487E-5 0.0 0.0 0.4859684225049696 2.787315299713046E-4 50-51 9.291050999043487E-5 0.0 0.0 0.5820146622075816 2.787315299713046E-4 52-53 9.291050999043487E-5 0.0 0.0 0.6708138821309397 2.787315299713046E-4 54-55 9.291050999043487E-5 0.0 0.0 0.7671156257360254 2.787315299713046E-4 56-57 9.291050999043487E-5 0.0 0.0 0.8778184983896286 2.787315299713046E-4 58-59 9.291050999043487E-5 0.0 0.0 0.9960238947249593 2.787315299713046E-4 60-61 9.291050999043487E-5 0.0 0.0 1.1224054159394483 2.787315299713046E-4 62-63 9.291050999043487E-5 0.0 0.0 1.2484385227414732 3.25186784966522E-4 64-65 9.291050999043487E-5 0.0 0.0 1.3758188319383595 3.25186784966522E-4 66-67 9.291050999043487E-5 0.0 0.0 1.5229890797632084 3.25186784966522E-4 68-69 9.291050999043487E-5 0.0 0.0 1.6858379761489428 3.25186784966522E-4 70-71 1.1613813748804358E-4 0.0 0.0 1.8589767115161182 3.25186784966522E-4 72-73 1.393657649856523E-4 0.0 0.0 2.0333697387681644 3.4841441246413074E-4 74-75 1.393657649856523E-4 0.0 0.0 2.20864541586512 3.716420399617395E-4 76-77 1.393657649856523E-4 0.0 0.0 2.3953490856908988 3.716420399617395E-4 78-79 1.393657649856523E-4 0.0 0.0 2.6045370989343626 3.716420399617395E-4 80-81 1.393657649856523E-4 0.0 0.0 2.8287766147962774 3.9486966745934816E-4 82-83 1.393657649856523E-4 0.0 0.0 3.0539452357580963 4.180972949569569E-4 84-85 1.393657649856523E-4 0.0 0.0 3.278951263327432 4.180972949569569E-4 86-87 1.393657649856523E-4 0.0 0.0 3.5135735286807774 4.180972949569569E-4 88-89 1.393657649856523E-4 0.0 0.0 3.763107930887588 4.180972949569569E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 8200 0.0 36.69566 1 GTATCAA 12270 0.0 32.9706 1 TATCAAC 14850 0.0 27.10488 2 ATCAACG 14920 0.0 26.94586 3 TCAACGC 14980 0.0 26.9331 4 CAACGCA 15180 0.0 26.546946 5 AACGCAG 15485 0.0 26.17751 6 TAACACG 95 0.0012236643 25.011337 4 GAGTACT 9430 0.0 23.418089 12-13 ACGCAGA 17220 0.0 23.415628 7 CGCAGAG 17190 0.0 23.40117 8 TAAGGTG 1140 0.0 23.343914 5 GTAAGGT 1045 0.0 22.737577 4 GTACATG 8450 0.0 22.326897 1 GTACTTT 9895 0.0 22.03744 14-15 TACATGG 8325 0.0 21.919844 2 GCAGAGT 18015 0.0 21.905672 9 TATTCCG 175 4.0360646E-6 21.72413 5 AGAGTAC 13940 0.0 21.62416 10-11 AGTACTT 9815 0.0 20.901041 12-13 >>END_MODULE