FastQCFastQC Report
Thu 26 May 2016
SRR938125_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938125_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2152609
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114950.5340031561700244No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102360.4755159901310456No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59870.2781276116563668No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT35190.16347604232817015No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26210.12175922334246489No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA91750.030.6539121
GTATCAA132650.030.4426161
ATCAACG156350.025.5807863
TATCAAC157200.025.5674922
TCAACGC157750.025.3514044
CAACGCA158950.025.1600135
AACGCAG162400.024.6547666
GAGTACT95650.023.28880312-13
TACATGG85250.023.0157932
GTACATG86650.022.9728971
GTACTTT98350.022.33555214-15
ACGCAGA179150.022.2965937
CGCAGAG179450.022.2593178
ACATGGG86400.021.936073
AGAGTAC138000.021.30447810-11
GTGTAAG9200.021.1722161
TAAGGTG12050.020.8904675
AGTACTT98200.020.87029812-13
GCAGAGT192350.020.6183369
ACTTTTT114400.019.3057316-17
Produced by FastQC (version 0.11.5)