FastQCFastQC Report
Thu 26 May 2016
SRR938124_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938124_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214234
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT118750.5363028478471562No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99290.44841692431784536No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58740.2652836150108796No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34960.15788755840620278No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26480.1195898897767806No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90850.032.1655161
GTATCAA133300.032.1336021
TATCAAC155900.027.2107942
ATCAACG156100.027.1454663
TCAACGC156550.027.0674344
CAACGCA159050.026.4625745
AACGCAG162450.025.933896
GAGTACT94750.024.5335512-13
ACGCAGA178900.023.4918377
GTACATG85350.023.4760861
GTACTTT98600.023.35732514-15
CGCAGAG179150.023.3242558
TACATGG84950.023.0651532
TAAGGTG11950.021.8885635
GTAAGGT11300.021.8850424
GCAGAGT187650.021.8614149
GTATTCG1553.8497325E-521.4938131
AGAGTAC137150.021.31549310-11
ACATGGG88500.021.0651863
AGTACTT97200.020.80961612-13
Produced by FastQC (version 0.11.5)