##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938124_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2214234 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.918758812302585 33.0 31.0 34.0 30.0 34.0 2 32.0347257787569 33.0 31.0 34.0 30.0 34.0 3 32.04984884163101 34.0 31.0 34.0 30.0 34.0 4 35.56930884450334 37.0 35.0 37.0 33.0 37.0 5 35.57343668284382 37.0 35.0 37.0 33.0 37.0 6 35.60416965867203 37.0 35.0 37.0 33.0 37.0 7 35.53866483849494 37.0 35.0 37.0 33.0 37.0 8 35.527960911087085 37.0 35.0 37.0 33.0 37.0 9 37.145326555368584 39.0 37.0 39.0 34.0 39.0 10-11 37.05490702428018 39.0 37.0 39.0 33.0 39.0 12-13 36.90140902000421 39.0 37.0 39.0 33.0 39.0 14-15 38.06163124583942 40.0 38.0 41.0 33.0 41.0 16-17 37.89043524758449 40.0 38.0 41.0 33.0 41.0 18-19 38.068427952962516 40.0 38.0 41.0 33.0 41.0 20-21 38.06546462568997 40.0 38.0 41.0 33.0 41.0 22-23 37.94868180147175 40.0 38.0 41.0 33.0 41.0 24-25 37.82516888458943 40.0 38.0 41.0 32.5 41.0 26-27 37.63575484795193 40.0 38.0 41.0 32.0 41.0 28-29 37.4518379719578 40.0 37.0 41.0 32.0 41.0 30-31 37.21960980637096 40.0 37.0 41.0 31.0 41.0 32-33 36.90963805090158 39.5 36.0 41.0 30.5 41.0 34-35 36.65902903667815 39.0 36.0 41.0 30.0 41.0 36-37 36.38359653948047 39.0 35.0 41.0 30.0 41.0 38-39 36.076298169028206 39.0 35.0 40.5 29.5 41.0 40-41 35.95883519989306 39.0 35.0 40.5 29.0 41.0 42-43 35.733039732927956 39.0 35.0 40.5 27.5 41.0 44-45 35.37921714687788 39.0 35.0 40.0 26.5 41.0 46-47 34.964010353015986 38.0 34.5 40.0 24.5 41.0 48-49 34.52877225261648 38.0 34.0 40.0 23.5 41.0 50-51 33.78303896516809 37.5 33.5 39.5 22.5 40.5 52-53 33.85306634258168 38.0 33.0 40.0 22.0 40.5 54-55 33.93526993985279 38.0 33.5 40.0 21.0 41.0 56-57 33.60592963525987 38.0 33.0 40.0 19.5 41.0 58-59 33.1802711456874 37.0 33.0 40.0 16.5 41.0 60-61 32.663099518840376 37.0 32.0 40.0 11.0 41.0 62-63 32.12194104146174 36.0 31.0 39.0 8.0 41.0 64-65 31.56846498608548 35.5 31.0 39.0 4.5 40.0 66-67 30.984564413697917 35.0 30.0 38.5 2.0 40.0 68-69 30.30476047247039 35.0 29.0 38.0 2.0 40.0 70-71 29.70093224112718 34.5 28.5 37.0 2.0 39.5 72-73 29.007627016837425 34.0 27.5 36.5 2.0 39.0 74-75 28.332215339480832 34.0 26.0 36.0 2.0 39.0 76-77 27.59512273770523 33.5 25.5 35.5 2.0 38.0 78-79 26.835102568201915 33.0 24.5 35.0 2.0 37.0 80-81 26.11449941605088 32.0 22.0 35.0 2.0 36.5 82-83 25.379461926788224 32.0 19.0 35.0 2.0 36.0 84-85 24.65343658348666 31.0 15.5 34.5 2.0 35.5 86-87 23.925413258038674 31.0 7.5 34.0 2.0 35.0 88-89 23.051150646227995 30.0 2.0 34.0 2.0 35.0 90-91 22.15389656197132 29.5 2.0 34.0 2.0 35.0 92-93 21.202473406153096 29.0 2.0 34.0 2.0 35.0 94-95 20.139416159267718 28.0 2.0 34.0 2.0 35.0 96-97 18.89119939446328 25.5 2.0 33.0 2.0 35.0 98-99 17.354574087472237 21.0 2.0 33.0 2.0 34.5 100-101 13.567538480576127 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.03254307945136148 1101 2 0.1342697661761676 1101 3 0.04730103236328631 1101 4 -0.03046663764026647 1101 5 0.1459519687150248 1101 6 0.07786934001072154 1101 7 0.07997618123077643 1101 8 0.13205725283477676 1101 9 0.07447863196284743 1101 10-11 0.16602072241931154 1101 12-13 0.17930487676930085 1101 14-15 0.17059200216642978 1101 16-17 0.18720876806035136 1101 18-19 0.18477929557119666 1101 20-21 0.14399918986212157 1101 22-23 0.22887680039232805 1101 24-25 0.2764103915676088 1101 26-27 0.11217790270652728 1101 28-29 0.16182736980544377 1101 30-31 0.09819798455588113 1101 32-33 0.23460770436951606 1101 34-35 0.09175252171225878 1101 36-37 0.12375383357407088 1101 38-39 0.015534774728976686 1101 40-41 0.17797491474150462 1101 42-43 0.20248795595086477 1101 44-45 0.2530439130982387 1101 46-47 0.2991685582063326 1101 48-49 0.34670608967896754 1101 50-51 0.47370287125153965 1101 52-53 0.3200445524191764 1101 54-55 0.3296614655556098 1101 56-57 0.24531414072166768 1101 58-59 0.09026967841251832 1101 60-61 0.28270352545714417 1101 62-63 0.140599311491016 1101 64-65 0.015054063985267874 1101 66-67 0.06970508242286755 1101 68-69 0.09295528962948296 1101 70-71 0.15942653223832437 1101 72-73 0.2005980237931695 1101 74-75 -0.039790662845131664 1101 76-77 0.04064960127153938 1101 78-79 -0.033573452608308685 1101 80-81 -0.10345759475011462 1101 82-83 -0.32549097043846587 1101 84-85 -0.1063764754711265 1101 86-87 -0.06434487992099847 1101 88-89 -0.25444303548200864 1101 90-91 -0.3403310597923621 1101 92-93 -0.3039351483497903 1101 94-95 -0.6772854497696343 1101 96-97 -0.636187431407155 1101 98-99 -0.9391254520883621 1101 100-101 -0.8689098987001582 1102 1 0.11511071458274458 1102 2 0.10801705799328687 1102 3 0.1503078097645414 1102 4 0.15320510281859612 1102 5 0.09092585905445105 1102 6 0.17735686848136112 1102 7 0.14720022433949254 1102 8 0.19853272641957886 1102 9 0.1593114190302316 1102 10-11 0.16325143182434232 1102 12-13 0.1564193426323115 1102 14-15 0.2095370607742879 1102 16-17 0.15484598265953053 1102 18-19 0.10502608741609265 1102 20-21 0.12480858933992778 1102 22-23 0.09672650318661624 1102 24-25 0.09222895720284896 1102 26-27 0.05083269966225146 1102 28-29 -0.012265041727253845 1102 30-31 -0.033941896561834994 1102 32-33 0.09717020089748729 1102 34-35 0.06621366275997786 1102 36-37 0.005414574644014181 1102 38-39 -0.16084431532158305 1102 40-41 -0.11115485582679696 1102 42-43 0.11385607438872825 1102 44-45 0.1601421298639778 1102 46-47 0.16023808212167268 1102 48-49 -0.031013898099487847 1102 50-51 -0.1977966287762385 1102 52-53 -0.1506195773513923 1102 54-55 -0.24607529906857195 1102 56-57 -0.2147665492400037 1102 58-59 -0.17940631181024713 1102 60-61 -0.2558082238901065 1102 62-63 -0.610741586236891 1102 64-65 -0.5533664404311516 1102 66-67 -0.5668428871343814 1102 68-69 -0.5778248059207662 1102 70-71 -0.8872014328747255 1102 72-73 -0.7572334855674256 1102 74-75 -0.7619901239861804 1102 76-77 -1.1879238113166473 1102 78-79 -1.2226455874897049 1102 80-81 -1.0604113750956508 1102 82-83 -1.3654776378458315 1102 84-85 -1.3835881581554226 1102 86-87 -1.1574473464506347 1102 88-89 -1.0707129649303688 1102 90-91 -1.0453460589590726 1102 92-93 -1.2496492753427333 1102 94-95 -1.5236634287708029 1102 96-97 -1.574683903825349 1102 98-99 -1.3919225189179798 1102 100-101 -1.36343450844406 1103 1 0.1862752471572513 1103 2 0.15571339859987177 1103 3 0.1780296734442146 1103 4 0.11096350438074154 1103 5 0.15385261774148518 1103 6 0.1482464870561131 1103 7 0.15324181469547682 1103 8 0.11743583389934997 1103 9 0.08295076419003777 1103 10-11 0.13996990502837292 1103 12-13 0.08472427426879392 1103 14-15 0.18177257173760353 1103 16-17 0.17449366518274445 1103 18-19 0.14250029386099072 1103 20-21 0.2674390301017624 1103 22-23 0.19313267721539518 1103 24-25 0.0635865422352282 1103 26-27 0.11826796973364395 1103 28-29 0.060142795602914134 1103 30-31 0.12985209912870488 1103 32-33 0.022915004058283728 1103 34-35 -0.023361243809667087 1103 36-37 -0.038099397218040565 1103 38-39 0.00941947958658318 1103 40-41 0.17398701361447166 1103 42-43 0.08838344781944585 1103 44-45 0.17452525491530224 1103 46-47 0.23296958538896462 1103 48-49 0.2557702859377784 1103 50-51 0.23249540054271023 1103 52-53 0.20184923900631446 1103 54-55 -0.005983869633091388 1103 56-57 0.08197762953100352 1103 58-59 -0.005555599627861341 1103 60-61 -0.051869614832419586 1103 62-63 0.05445578741542079 1103 64-65 -0.3246522010852111 1103 66-67 -0.5618645843535646 1103 68-69 -0.5060946726611242 1103 70-71 -0.828099094215613 1103 72-73 -0.536687802786112 1103 74-75 -0.5857213678514519 1103 76-77 -0.4781482657577065 1103 78-79 -0.4642916041044707 1103 80-81 -0.4631990995142452 1103 82-83 -0.5655617153848773 1103 84-85 -0.7357370888064416 1103 86-87 -0.7953886068837441 1103 88-89 -0.4364314889719836 1103 90-91 -0.5767729395259522 1103 92-93 -0.7755596265261424 1103 94-95 -0.7682219174086029 1103 96-97 -1.1175746590762863 1103 98-99 -1.062520147279617 1103 100-101 -1.1582087960987408 1104 1 0.16217309217387665 1104 2 0.14655552626884827 1104 3 0.09546635265000702 1104 4 0.04029459282342884 1104 5 0.12197287294448245 1104 6 0.08428360784551359 1104 7 0.10933829709875198 1104 8 0.1365047026429096 1104 9 0.06610995831086797 1104 10-11 0.1679699564825441 1104 12-13 0.1259030263157328 1104 14-15 0.1950745681838697 1104 16-17 0.2756381322449144 1104 18-19 0.28264374907195133 1104 20-21 0.3191328797016908 1104 22-23 0.3067710431958375 1104 24-25 0.3175977228391389 1104 26-27 0.19947891947334995 1104 28-29 0.2205468176689891 1104 30-31 0.22801986934989316 1104 32-33 0.10779849554443643 1104 34-35 0.2334009148483105 1104 36-37 0.1022706262591484 1104 38-39 0.13483369176369564 1104 40-41 0.223526947757982 1104 42-43 0.260759788510164 1104 44-45 0.014867318387906892 1104 46-47 -0.04911506595196613 1104 48-49 0.1475939505381021 1104 50-51 -0.01378394077589462 1104 52-53 0.05019303347503978 1104 54-55 -0.14815442517556932 1104 56-57 0.0033785859476225255 1104 58-59 -8.272764587573533E-4 1104 60-61 0.10897965574397261 1104 62-63 0.21555237782392567 1104 64-65 0.08749003767138319 1104 66-67 0.10824085544492945 1104 68-69 0.27954092353136417 1104 70-71 0.26787459231341515 1104 72-73 0.22033162674727436 1104 74-75 0.04193559104661304 1104 76-77 -0.05616408112070914 1104 78-79 -0.1447818070596405 1104 80-81 -0.1711049121613435 1104 82-83 -0.1644684112477428 1104 84-85 -0.1482791896075959 1104 86-87 -0.27508932804313346 1104 88-89 -0.2200138936828573 1104 90-91 -0.3344675837530957 1104 92-93 -0.486371871940225 1104 94-95 -0.8237407296132417 1104 96-97 -1.032340939407856 1104 98-99 -1.0092182333542006 1104 100-101 -1.1371311515058355 1105 1 0.14981245068032578 1105 2 0.15743982016155655 1105 3 0.1479421088955135 1105 4 0.05939323851089284 1105 5 0.06834002841651454 1105 6 0.14659155341180252 1105 7 0.1560261177185538 1105 8 0.20353033952896737 1105 9 0.22585208385233813 1105 10-11 0.2916598141944675 1105 12-13 0.27405420195189123 1105 14-15 0.2610512749514413 1105 16-17 0.19911135618355758 1105 18-19 0.18227353977570715 1105 20-21 0.24334620478749258 1105 22-23 0.3636328737489549 1105 24-25 0.2602088196400132 1105 26-27 0.3139145651390436 1105 28-29 0.25960642664887956 1105 30-31 0.18558286864606544 1105 32-33 0.04035258369628991 1105 34-35 0.1610165737922884 1105 36-37 0.20667499902342001 1105 38-39 0.1400921739033123 1105 40-41 0.1509633634553822 1105 42-43 0.16332572190831485 1105 44-45 0.18769975622304003 1105 46-47 0.3583411129856273 1105 48-49 0.4773348607173986 1105 50-51 0.29336188998764356 1105 52-53 0.13591665467385639 1105 54-55 0.1413288599665421 1105 56-57 0.15864178441808008 1105 58-59 0.3402577698701279 1105 60-61 0.2557912120243131 1105 62-63 0.3081912057431566 1105 64-65 0.18169625198145667 1105 66-67 0.1370399506349962 1105 68-69 0.13117377168727629 1105 70-71 0.1504881787927843 1105 72-73 0.008150024428289271 1105 74-75 -0.05889593435989582 1105 76-77 -0.19992974931594887 1105 78-79 -0.20123481335775395 1105 80-81 -0.1867072374938452 1105 82-83 -0.3790645255353233 1105 84-85 -0.5869020391421316 1105 86-87 -0.4563542479743461 1105 88-89 -0.6227256251930235 1105 90-91 -0.5714262487412327 1105 92-93 -0.6695432779147303 1105 94-95 -0.7889905545648084 1105 96-97 -0.7213057229282676 1105 98-99 -0.5879871939111609 1105 100-101 -1.0708003798052719 1106 1 0.3348264214337142 1106 2 0.3680917692898191 1106 3 0.4053575969714842 1106 4 0.35984213246870667 1106 5 0.300038631922078 1106 6 0.39604526113163274 1106 7 0.4479574993418325 1106 8 0.3739747411585981 1106 9 0.5392035930422452 1106 10-11 0.485610758745203 1106 12-13 0.5232916437646296 1106 14-15 0.5239132268680393 1106 16-17 0.07546123303180252 1106 18-19 0.48080290740306 1106 20-21 0.6620324021091406 1106 22-23 0.5482008792714197 1106 24-25 0.42812289805436876 1106 26-27 0.3915110630594043 1106 28-29 0.42827132413859914 1106 30-31 0.3525570919767418 1106 32-33 0.41027584137003714 1106 34-35 0.3738101242031817 1106 36-37 0.4708347884261741 1106 38-39 0.3762509138052508 1106 40-41 0.2905981633144137 1106 42-43 0.11263974629856222 1106 44-45 0.19244453582786747 1106 46-47 0.4133291705571338 1106 48-49 0.548438056466729 1106 50-51 0.2957903128706292 1106 52-53 0.3342246250709664 1106 54-55 0.1757110244554596 1106 56-57 0.11174512152083338 1106 58-59 0.20684336808558612 1106 60-61 0.44131795202694235 1106 62-63 0.2141787095190324 1106 64-65 0.2567445154642485 1106 66-67 0.08620741765898643 1106 68-69 1.2316715931959266E-4 1106 70-71 -0.19633371685352685 1106 72-73 -0.19108594091882125 1106 74-75 -0.09842454971591508 1106 76-77 -0.5605699109235829 1106 78-79 -0.36487210336077425 1106 80-81 -0.36888312666500767 1106 82-83 -0.6282932537804484 1106 84-85 -0.28785675569945823 1106 86-87 -0.45021021463817235 1106 88-89 -0.9358260142054071 1106 90-91 -0.6592853929527109 1106 92-93 -0.6936082152932244 1106 94-95 -0.7078770429196091 1106 96-97 -0.8662990037994724 1106 98-99 -1.0691202626747653 1106 100-101 -1.3668146729606665 1107 1 -0.19153193865923512 1107 2 -0.1433462087104722 1107 3 -0.0818921294336441 1107 4 -0.05893045669530039 1107 5 -0.007379262894893657 1107 6 -0.08187617258620605 1107 7 -0.04757696554106161 1107 8 -0.06293505575187908 1107 9 -0.14991410412329742 1107 10-11 -0.20278120580012882 1107 12-13 -0.21226508826160284 1107 14-15 -0.18956155779528672 1107 16-17 -0.06086536017161137 1107 18-19 -0.12887553436807764 1107 20-21 -0.016047048052968194 1107 22-23 0.05111493459854444 1107 24-25 0.04543680173458142 1107 26-27 -0.009764760490064361 1107 28-29 -0.07868301360257846 1107 30-31 -0.1010020190215215 1107 32-33 -0.1066787515881984 1107 34-35 -0.2129439357658498 1107 36-37 -0.09255218629839135 1107 38-39 -0.02101374825136304 1107 40-41 -0.04787114072610876 1107 42-43 -0.05167401537625693 1107 44-45 0.0157707427155529 1107 46-47 0.01955672863373792 1107 48-49 -0.11577579972566099 1107 50-51 -0.15485474280049516 1107 52-53 -0.1798063146535327 1107 54-55 -0.23196005877519354 1107 56-57 -0.22137902107525775 1107 58-59 -0.22429023412001214 1107 60-61 -0.14938979106239714 1107 62-63 -0.12726343702659904 1107 64-65 -0.1642362466226217 1107 66-67 -0.06360052604842181 1107 68-69 -0.07160842389220434 1107 70-71 -0.21716608221616696 1107 72-73 -0.4574446456638732 1107 74-75 -0.5777894867743711 1107 76-77 -0.5972453662188251 1107 78-79 -0.7557375793198737 1107 80-81 -0.8888137250748542 1107 82-83 -0.9067695337540016 1107 84-85 -0.8183602117093081 1107 86-87 -0.7868251594949278 1107 88-89 -0.8196901444943769 1107 90-91 -0.9391511880422065 1107 92-93 -0.7473415501098692 1107 94-95 -0.7843664689713385 1107 96-97 -0.7604491451613669 1107 98-99 -0.7504577658622509 1107 100-101 -0.7767138883972411 1108 1 -0.13823903493836198 1108 2 -0.08501926557285344 1108 3 -0.11481824389174733 1108 4 0.04605580903375284 1108 5 -0.06956636251127435 1108 6 -0.13023759886726083 1108 7 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2316 98-99 -0.14402038870878897 2316 100-101 0.00585849224273538 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7650.0 3 2418.0 4 2282.0 5 2538.0 6 2833.0 7 3739.0 8 4651.0 9 5865.0 10 7444.0 11 9258.0 12 11362.0 13 14055.0 14 16458.0 15 20038.0 16 23351.0 17 29469.0 18 29089.0 19 24616.0 20 24784.0 21 26610.0 22 28839.0 23 31895.0 24 35632.0 25 39861.0 26 44799.0 27 50930.0 28 57833.0 29 67077.0 30 77987.0 31 92922.0 32 112009.0 33 139252.0 34 177860.0 35 234146.0 36 291367.0 37 304957.0 38 149493.0 39 8865.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.127591905629 18.263171082279747 14.885993691593647 22.723243320497616 2 15.773090037567993 19.699022256122085 39.93621484157187 24.59167286473805 3 19.63183302358 23.664038927151353 29.463761121607092 27.24036692766155 4 13.004353099401348 16.55855520151513 36.34182276653802 34.0952689325455 5 13.94265664396294 37.00638395305523 33.70014968894745 15.35080971403438 6 32.45227825754829 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22.45956034840315 28-29 22.094932530207657 26.410896461905796 29.383501555603793 22.110669452282757 30-31 22.294556506073253 25.76865913711981 29.299860643481267 22.63692371332567 32-33 22.08515702969661 25.811376247684155 29.346584986775326 22.756881735843905 34-35 22.820149199078877 25.97666085915596 29.03975357107661 22.163436370688554 36-37 22.807872832994423 25.564783756558114 29.202204733165317 22.42513867728215 38-39 22.32400197464368 26.009581766402363 29.060903297813095 22.605512961140864 40-41 22.676218483692352 25.85832475650662 29.030285707184312 22.435171052616713 42-43 22.36494933926727 25.882605747402 28.772779027195526 22.979665886135198 44-45 22.529112813975505 26.01695771009242 28.902203670985273 22.551725804946802 46-47 22.97083608875582 26.453637394910807 28.18074788285273 22.394778633480648 48-49 22.822331452107353 26.35499552020921 28.119441207659424 22.70323182002401 50-51 22.75166464491002 26.70343258806616 28.120484796834354 22.424417970189463 52-53 22.984571092080813 26.61947967669925 27.74745895212989 22.648490279090055 54-55 22.57651061268588 26.635326938866065 27.942476906399694 22.84568554204836 56-57 22.823329312751568 26.751324759103905 27.77966760179074 22.645678326353785 58-59 22.993947902755245 26.342246359015185 27.71563208797694 22.948173650252627 60-61 22.58712273696395 26.637511673518294 28.158220393314547 22.617145196203207 62-63 22.42861703476047 26.638223208695887 28.30660282402689 22.62655693251675 64-65 22.288612824465556 26.680430229174835 28.11923380676735 22.91172313959225 66-67 22.39217555527443 26.660604883161948 27.876786908354866 23.070432653208755 68-69 22.56944256142996 26.39097526893966 28.131332577717284 22.9082495919131 70-71 22.63528550545525 26.452199610936262 27.751473674935212 23.161041208673275 72-73 22.624510654718964 26.544876071690737 27.77849951209505 23.052113761495253 74-75 22.437670564421392 27.163253872151234 27.437580199165023 22.961495364262348 76-77 22.717854225993435 26.584815297995057 27.54584122349268 23.15148925251883 78-79 22.791710380545073 26.691458882227188 27.554368018719515 22.96246271850822 80-81 22.93801468743076 26.74846628538284 27.256199739662097 23.05731928752431 82-83 22.82730145063773 26.79034968032757 27.422286296490956 22.960062572543745 84-85 22.80525071201263 26.904839879031258 27.27294080978591 23.016968599170205 86-87 22.94136792882197 26.799615388177184 27.11203908639107 23.146977596609776 88-89 22.85134276178087 26.899817717283508 26.893038888109324 23.3558006328263 90-91 22.920807433758274 26.712401231966755 26.966137191036232 23.400654143238743 92-93 23.075862626945643 26.420287166029315 27.051294886783143 23.4525553202419 94-95 22.884025857656336 26.402312318996973 27.138245353949554 23.57541646939714 96-97 22.99731570649525 26.186651385092286 27.167630841513247 23.64840206689922 98-99 22.85806018821555 26.363710925028972 26.701993703855255 24.076235182900227 100-101 23.742372016754203 25.19565714843895 26.728331191272332 24.333639643534518 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1173.0 1 811.0 2 635.0 3 824.5 4 665.0 5 601.0 6 1149.0 7 2530.5 8 4268.5 9 4972.0 10 3995.5 11 2867.0 12 2617.5 13 2725.0 14 2963.0 15 3124.5 16 3187.0 17 3348.5 18 3652.0 19 3954.5 20 4358.0 21 4990.5 22 5855.5 23 6993.0 24 8437.5 25 10414.0 26 13079.5 27 16251.0 28 18746.0 29 21081.0 30 23924.0 31 27575.5 32 32700.0 33 35492.0 34 36723.0 35 39463.0 36 43488.5 37 47553.5 38 50082.0 39 52359.5 40 55531.5 41 59618.5 42 62403.5 43 64602.5 44 67969.0 45 72536.0 46 75524.0 47 79388.5 48 84855.0 49 84902.5 50 81669.0 51 79317.5 52 79141.0 53 80921.5 54 82836.5 55 79412.0 56 72493.0 57 66862.5 58 62742.5 59 56535.5 60 46894.0 61 39196.0 62 32355.5 63 24897.5 64 18583.5 65 14001.5 66 10391.5 67 7849.5 68 5996.0 69 4414.5 70 3301.5 71 2408.0 72 1694.5 73 1245.0 74 894.0 75 620.5 76 429.0 77 264.0 78 171.0 79 101.5 80 55.5 81 35.0 82 29.5 83 18.0 84 11.5 85 10.0 86 9.0 87 10.0 88 11.0 89 8.0 90 4.5 91 4.0 92 4.0 93 3.0 94 2.0 95 1.5 96 0.5 97 0.5 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14524210178328034 2 0.05329156719660163 3 0.1506164208480224 4 0.13259664516035793 5 0.19501100606349644 6 0.20309506583315046 7 0.1660167805209386 8 0.0729823496522951 9 0.12013183791776298 10-11 0.10357983844525918 12-13 0.15185838533777368 14-15 0.13754192194682224 16-17 0.13578059048862948 18-19 0.13499024944969681 20-21 0.13754192194682224 22-23 0.19833043842701356 24-25 0.2170050681183651 26-27 0.15115836898900478 28-29 0.12979657976528225 30-31 0.10303789030427679 32-33 0.12812557299725325 34-35 0.09737001599650262 36-37 0.09003113492069943 38-39 0.05313349898881509 40-41 0.06284340318141624 42-43 0.06437892291419967 44-45 0.0408493411265476 46-47 0.05299801195356949 48-49 0.04340101362367302 50-51 0.055301291552744644 52-53 0.028429696229034507 54-55 0.05306575547119229 56-57 0.028181303331084248 58-59 0.05417223292569801 60-61 0.04023964946794241 62-63 0.05367544712979748 64-65 0.040736435263842935 66-67 0.04127838340482533 68-69 0.0663886472703427 70-71 0.05550452210561304 72-73 0.054217395270779865 74-75 0.04489137101137459 76-77 0.022852146611424085 78-79 0.02264891605855569 80-81 0.02585544255936816 82-83 0.021835993847082104 84-85 0.019826269490939078 86-87 0.02590060490445003 88-89 0.06604992968222871 90-91 0.11227358987351835 92-93 0.08221804922153667 94-95 0.07842441223465993 96-97 0.0698887290141873 98-99 0.058372331018311525 100-101 0.13404184020297763 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2214234.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 53.595919096190904 #Duplication Level Percentage of deduplicated Percentage of total 1 77.49409995457854 41.533675115977225 2 12.543235209722651 13.445324390095765 3 4.064184447787535 6.534711025668538 4 1.8245925780213608 3.9116286478057294 5 0.9647619611095339 2.5853652007354526 6 0.6257242088771712 2.0121758445305353 7 0.4159466082386314 1.5605128534444885 8 0.3197722884063929 1.3710791758906284 9 0.23857090621401872 1.1507784289336345 >10 1.3617028828380848 13.65265739894842 >50 0.0948759276869266 3.4659799548640473 >100 0.04637151062100752 4.393931895443966 >500 0.004135812041205347 1.6120302950431178 >1k 0.0017724908748022743 1.5180724748437364 >5k 1.6880865474307375E-4 0.7148846570104168 >10k+ 8.440432737153688E-5 0.5371926407643295 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 11875 0.5363028478471562 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9929 0.44841692431784536 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5874 0.2652836150108796 No Hit GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT 3496 0.15788755840620278 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2648 0.1195898897767806 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.5162345081865785E-5 2 0.0 0.0 0.0 0.0 4.5162345081865785E-5 3 0.0 0.0 0.0 0.0 4.5162345081865785E-5 4 0.0 0.0 0.0 0.0 4.5162345081865785E-5 5 0.0 0.0 0.0 0.0 4.5162345081865785E-5 6 0.0 0.0 0.0 0.0 4.5162345081865785E-5 7 0.0 0.0 0.0 4.5162345081865785E-5 4.5162345081865785E-5 8 0.0 0.0 0.0 4.5162345081865785E-5 4.5162345081865785E-5 9 0.0 0.0 0.0 4.5162345081865785E-5 4.5162345081865785E-5 10-11 0.0 0.0 0.0 4.5162345081865785E-5 4.5162345081865785E-5 12-13 0.0 0.0 0.0 4.5162345081865785E-5 4.5162345081865785E-5 14-15 0.0 0.0 0.0 9.032469016373157E-5 4.5162345081865785E-5 16-17 0.0 0.0 0.0 1.3548703524559735E-4 4.5162345081865785E-5 18-19 0.0 0.0 0.0 1.8064938032746314E-4 4.5162345081865785E-5 20-21 0.0 0.0 0.0 2.0323055286839602E-4 4.5162345081865785E-5 22-23 0.0 0.0 0.0 3.838799331958592E-4 1.1290586270466446E-4 24-25 0.0 0.0 0.0 7.451786938507854E-4 1.8064938032746314E-4 26-27 0.0 0.0 0.0 0.0011968021446694432 1.8064938032746314E-4 28-29 4.5162345081865785E-5 0.0 0.0 0.0032516888458943363 1.8064938032746314E-4 30-31 4.5162345081865785E-5 0.0 0.0 0.01038733936882913 2.0323055286839602E-4 32-33 4.5162345081865785E-5 0.0 0.0 0.02468122158723965 2.258117254093289E-4 34-35 4.5162345081865785E-5 0.0 0.0 0.04739788116341814 3.1613641557306046E-4 36-37 4.5162345081865785E-5 0.0 0.0 0.08506327696169419 3.1613641557306046E-4 38-39 4.5162345081865785E-5 0.0 0.0 0.13801612657018184 3.1613641557306046E-4 40-41 4.5162345081865785E-5 0.0 0.0 0.19444647674997312 3.1613641557306046E-4 42-43 4.5162345081865785E-5 0.0 0.0 0.2502219729260774 3.1613641557306046E-4 44-45 4.5162345081865785E-5 0.0 0.0 0.3217817087082937 3.1613641557306046E-4 46-47 4.5162345081865785E-5 0.0 0.0 0.40460944958843553 3.1613641557306046E-4 48-49 4.5162345081865785E-5 0.0 0.0 0.4898985382755391 3.1613641557306046E-4 50-51 4.5162345081865785E-5 0.0 0.0 0.5809232447880396 3.1613641557306046E-4 52-53 4.5162345081865785E-5 0.0 0.0 0.666912349823912 3.1613641557306046E-4 54-55 4.5162345081865785E-5 0.0 0.0 0.7615952062880437 3.1613641557306046E-4 56-57 4.5162345081865785E-5 0.0 0.0 0.8723106952562376 3.1613641557306046E-4 58-59 4.5162345081865785E-5 0.0 0.0 0.9882650162539279 3.1613641557306046E-4 60-61 4.5162345081865785E-5 0.0 0.0 1.1122582346761904 3.1613641557306046E-4 62-63 4.5162345081865785E-5 0.0 0.0 1.2360482225455847 3.1613641557306046E-4 64-65 4.5162345081865785E-5 0.0 0.0 1.3657544776207033 3.1613641557306046E-4 66-67 4.5162345081865785E-5 0.0 0.0 1.5123514497564394 3.1613641557306046E-4 68-69 4.5162345081865785E-5 0.0 0.0 1.6724293818991127 3.1613641557306046E-4 70-71 4.5162345081865785E-5 0.0 0.0 1.8432107898261882 3.612987606549263E-4 72-73 4.5162345081865785E-5 0.0 0.0 2.0180116464655495 4.0646110573679204E-4 74-75 4.5162345081865785E-5 0.0 0.0 2.193580262971303 4.0646110573679204E-4 76-77 4.5162345081865785E-5 0.0 0.0 2.3819298231352244 4.0646110573679204E-4 78-79 4.5162345081865785E-5 0.0 0.0 2.595344484819581 4.0646110573679204E-4 80-81 4.5162345081865785E-5 0.0 0.0 2.8202303821547314 4.290422782777249E-4 82-83 4.5162345081865785E-5 0.0 0.0 3.043467853894394 4.516234508186578E-4 84-85 4.5162345081865785E-5 0.0 0.0 3.2671117867397936 4.516234508186578E-4 86-87 4.5162345081865785E-5 0.0 0.0 3.5013688706794315 4.516234508186578E-4 88-89 4.5162345081865785E-5 0.0 0.0 3.753781217341979 4.516234508186578E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 9085 0.0 32.165516 1 GTATCAA 13330 0.0 32.133602 1 TATCAAC 15590 0.0 27.210794 2 ATCAACG 15610 0.0 27.145466 3 TCAACGC 15655 0.0 27.067434 4 CAACGCA 15905 0.0 26.462574 5 AACGCAG 16245 0.0 25.93389 6 GAGTACT 9475 0.0 24.53355 12-13 ACGCAGA 17890 0.0 23.491837 7 GTACATG 8535 0.0 23.476086 1 GTACTTT 9860 0.0 23.357325 14-15 CGCAGAG 17915 0.0 23.324255 8 TACATGG 8495 0.0 23.065153 2 TAAGGTG 1195 0.0 21.888563 5 GTAAGGT 1130 0.0 21.885042 4 GCAGAGT 18765 0.0 21.861414 9 GTATTCG 155 3.8497325E-5 21.493813 1 AGAGTAC 13715 0.0 21.315493 10-11 ACATGGG 8850 0.0 21.065186 3 AGTACTT 9720 0.0 20.809616 12-13 >>END_MODULE