FastQCFastQC Report
Thu 26 May 2016
SRR938124_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938124_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2214234
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT114470.5169733641521177No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100690.4547396526293066No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62170.28077429937395953No Hit
GAGTAAGCAGTGGTATCAACGCAGAGTAAGCAGTGGTATCAACGCAGAGT34660.1565326880537468No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25060.11317683677515564No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA90550.033.1564641
GTATCAA132400.031.2872981
TATCAAC158450.026.0200542
ATCAACG159450.025.7937953
TCAACGC160500.025.623894
CAACGCA162800.025.2618855
AACGCAG165450.024.914116
GAGTACT98650.023.58961512-13
GTACTTT101950.022.56932614-15
ACGCAGA184100.022.3391327
CGCAGAG185100.022.141488
GTACATG89500.021.9213141
TACATGG88450.021.9100322
GTAAGGT12300.021.2353694
GCAGAGT194000.020.9053969
AGAGTAC144450.020.6802110-11
TAAGGTG12850.020.3264625
AGTACTT102250.020.25093312-13
ACATGGG91650.020.0539683
ACTTTTT117050.019.59647416-17
Produced by FastQC (version 0.11.5)