FastQCFastQC Report
Thu 26 May 2016
SRR938123_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938123_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102994
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102310.4864968706520323No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.4046611640356558No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52330.24883570756740153No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25380.12068508041392415No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23390.11122238104340765No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21850.10389948806320892No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21260.10109396412923671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47600.051.4909631
GTATCAA85350.040.5609361
TATCAAC105750.032.4491272
ATCAACG106350.032.0872963
TCAACGC107450.031.758814
CAACGCA109600.031.1358015
AACGCAG113250.030.039086
ACGCAGA127850.026.34427
CGCAGAG129200.025.9953948
GTGTAGC10950.025.2574061
TAAGGTG13350.024.5648445
AGGTAAG10750.024.3164632
GTGGTAT23550.023.892741
GCAGAGT139950.023.419889
TGGTATC22900.023.0374092
TACATGG75750.023.0265882
GTACATG78850.022.9198821
GAGTACT82300.022.62639612-13
GTAAGGT12350.021.935834
GTACTTT85450.021.51459714-15
Produced by FastQC (version 0.11.5)