FastQCFastQC Report
Thu 26 May 2016
SRR938123_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938123_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2102994
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT99240.47189863594475306No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86460.4111281344597274No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54210.2577753431536181No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24620.11707118517694295No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23730.11283912364942553No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC23650.11245871362448015No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23210.11036645848728052No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22210.10561133317546319No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21650.10294846300084547No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA47400.052.9724771
GTATCAA81550.038.8952521
ATCAACG102100.031.1065883
TATCAAC102300.031.0524252
TCAACGC103350.030.7296264
CAACGCA104500.030.390015
AACGCAG108300.029.3828166
TGGTATC22350.026.5966662
GTGGTAT23900.025.6676731
ACGCAGA124650.025.3763457
CGCAGAG125900.025.011228
GTAAGGT10700.023.5495954
GAGTACT84200.022.7039212-13
GCAGAGT139650.022.4125659
TACATGG75250.022.3081172
GTACATG76900.022.1386621
TAAGGTG12000.021.3935665
AGGTAAG10950.021.2802472
GTACTTT89750.021.1411914-15
CGCGCGT1353.8001384E-421.108857
Produced by FastQC (version 0.11.5)