##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938123_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2102994 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46152390353943 33.0 31.0 34.0 31.0 34.0 2 32.69155213947353 34.0 31.0 34.0 31.0 34.0 3 32.75613482492104 34.0 31.0 34.0 31.0 34.0 4 36.224303540571206 37.0 35.0 37.0 35.0 37.0 5 36.14487868248792 37.0 35.0 37.0 35.0 37.0 6 36.1995074641202 37.0 37.0 37.0 35.0 37.0 7 36.17014171224454 37.0 37.0 37.0 35.0 37.0 8 36.16331002370905 37.0 37.0 37.0 35.0 37.0 9 37.84755876621617 39.0 38.0 39.0 35.0 39.0 10-11 37.82103182415166 39.0 38.0 39.0 35.0 39.0 12-13 37.7386778088763 39.0 38.0 39.0 35.0 39.0 14-15 38.969928349771806 40.0 38.0 41.0 35.5 41.0 16-17 38.87731467612366 40.0 38.0 41.0 35.0 41.0 18-19 39.127940212858434 40.0 38.5 41.0 36.0 41.0 20-21 39.098221630684634 40.0 39.0 41.0 36.0 41.0 22-23 38.99116069755786 40.0 38.5 41.0 35.0 41.0 24-25 38.878790429264185 40.0 38.0 41.0 35.0 41.0 26-27 38.711692710487995 40.0 38.0 41.0 35.0 41.0 28-29 38.583833097003605 40.0 38.0 41.0 34.0 41.0 30-31 38.335220880325856 40.0 38.0 41.0 34.0 41.0 32-33 38.09919286502957 40.0 37.5 41.0 33.5 41.0 34-35 38.01347008122705 40.0 37.5 41.0 33.0 41.0 36-37 37.95336196869796 40.0 37.5 41.0 33.0 41.0 38-39 37.75920021645331 40.0 37.0 41.0 33.0 41.0 40-41 37.53901081030188 40.0 37.0 41.0 33.0 41.0 42-43 37.27227181817922 40.0 36.5 41.0 32.0 41.0 44-45 36.985566292628505 40.0 36.0 41.0 31.0 41.0 46-47 36.600031193622044 39.0 36.0 41.0 30.5 41.0 48-49 36.161768174326696 39.0 35.0 40.5 29.5 41.0 50-51 35.756845716155155 39.0 35.0 40.0 28.5 41.0 52-53 35.20555431922297 38.0 34.5 40.0 27.0 41.0 54-55 34.80404556551279 38.0 34.0 40.0 26.0 41.0 56-57 34.40058079100558 38.0 34.0 40.0 25.5 41.0 58-59 33.86851793205307 37.0 33.0 40.0 23.5 41.0 60-61 33.36618578084388 36.5 32.5 39.0 22.5 41.0 62-63 32.78314726527988 36.0 32.0 39.0 20.5 40.0 64-65 32.183208321088884 35.0 31.0 39.0 18.5 40.0 66-67 31.606610622759742 35.0 31.0 38.0 14.5 40.0 68-69 30.96615373129928 35.0 30.0 37.5 8.0 40.0 70-71 30.337229920770103 34.0 29.0 37.0 7.0 39.0 72-73 29.656968588593216 34.0 28.5 36.0 2.0 39.0 74-75 28.998992388946427 34.0 27.5 36.0 2.0 38.5 76-77 27.7275898076742 31.5 26.0 34.5 2.0 36.5 78-79 28.382882690107532 33.0 27.0 35.0 2.0 37.0 80-81 28.115456106864784 33.0 27.0 35.0 2.0 36.5 82-83 27.57070419601768 33.0 26.0 35.0 2.0 36.0 84-85 26.920318840662407 33.0 25.5 35.0 2.0 36.0 86-87 26.21207288275668 32.0 23.5 35.0 2.0 35.0 88-89 25.39380283538612 32.0 20.5 34.0 2.0 35.0 90-91 24.740129073121466 31.0 18.5 34.0 2.0 35.0 92-93 23.794942829128374 31.0 8.5 34.0 2.0 35.0 94-95 22.89173174055656 30.5 2.0 34.0 2.0 35.0 96-97 21.824978340404208 30.0 2.0 34.0 2.0 35.0 98-99 20.40250828105073 29.0 2.0 34.0 2.0 35.0 100-101 16.59860846012875 20.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.16393848391957988 1101 2 0.18171970919627967 1101 3 0.15962151713448947 1101 4 0.11374023712394177 1101 5 0.025348579764262524 1101 6 0.06252835290460723 1101 7 0.0893529310882144 1101 8 0.05555492443227905 1101 9 -0.027736888047101615 1101 10-11 0.0776781161503095 1101 12-13 0.209453244574064 1101 14-15 0.3616368893705939 1101 16-17 0.3584918155973398 1101 18-19 0.24964917493471006 1101 20-21 0.13228400921334327 1101 22-23 0.23394356790134196 1101 24-25 0.29226635200355844 1101 26-27 0.3721297233850791 1101 28-29 0.31925098548642694 1101 30-31 0.3553527803173182 1101 32-33 0.3879780386417906 1101 34-35 0.36302914310601153 1101 36-37 0.4279725074427745 1101 38-39 0.4509949658353136 1101 40-41 0.5320643757878045 1101 42-43 0.7070808615669648 1101 44-45 0.6829901289116833 1101 46-47 0.7532758526888017 1101 48-49 0.8501258511289933 1101 50-51 0.7533331027705898 1101 52-53 0.9880954421872872 1101 54-55 1.223946817899801 1101 56-57 1.1859621994226544 1101 58-59 1.2822839100243897 1101 60-61 1.2379594014431845 1101 62-63 1.2578166974226974 1101 64-65 1.236829533842787 1101 66-67 1.4965111708081729 1101 68-69 1.877026225521444 1101 70-71 1.8894304197459881 1101 72-73 1.9899407130721478 1101 74-75 1.9593478141017506 1101 76-77 2.042838047506578 1101 78-79 2.2501739221858905 1101 80-81 2.33572776465979 1101 82-83 2.3957871803022996 1101 84-85 2.5209608544626256 1101 86-87 2.758991842567138 1101 88-89 3.1827835964797124 1101 90-91 3.3692514358674117 1101 92-93 3.6152119395680913 1101 94-95 3.8481190977617494 1101 96-97 4.037887707804856 1101 98-99 4.419769171399391 1101 100-101 4.5576154977872285 1102 1 0.15005043398501527 1102 2 0.09040568299852936 1102 3 0.12956905452388412 1102 4 0.085366801773759 1102 5 -0.0377379891362537 1102 6 0.02206950081784953 1102 7 0.02140993523824619 1102 8 0.0637671686910295 1102 9 0.06281145250123643 1102 10-11 0.12957359362717824 1102 12-13 0.23777074963575018 1102 14-15 0.32269039228455654 1102 16-17 0.24485013858357263 1102 18-19 0.1395635456630302 1102 20-21 0.17933480510134814 1102 22-23 0.17977955792337497 1102 24-25 0.3050131444015207 1102 26-27 0.244115919789877 1102 28-29 0.22763074095920643 1102 30-31 0.25655080709674394 1102 32-33 0.2645569506858223 1102 34-35 0.3301038212272047 1102 36-37 0.31417366631834653 1102 38-39 0.3458286850876391 1102 40-41 0.4066899225063665 1102 42-43 0.4168966271966781 1102 44-45 0.29403020855642126 1102 46-47 0.1799174066559388 1102 48-49 0.2681555389261234 1102 50-51 0.46072876481741076 1102 52-53 0.5301343249238357 1102 54-55 0.6815768278553946 1102 56-57 0.7418332018052851 1102 58-59 0.8145788975519324 1102 60-61 0.7174643065294788 1102 62-63 0.7968280736433755 1102 64-65 0.7950003896880631 1102 66-67 0.96833405077059 1102 68-69 1.330890169501668 1102 70-71 1.4898482190009936 1102 72-73 1.803963834653409 1102 74-75 1.9458842750568195 1102 76-77 1.843532701689604 1102 78-79 1.8630996915070064 1102 80-81 2.088645647810228 1102 82-83 2.0168194856369297 1102 84-85 2.1787589349351215 1102 86-87 2.271935229929131 1102 88-89 2.5795824264180744 1102 90-91 2.888649458172413 1102 92-93 2.9006549633832073 1102 94-95 3.080602290710061 1102 96-97 3.1819825879462478 1102 98-99 3.6688127634662386 1102 100-101 3.503709885742083 1103 1 0.18854371142185755 1103 2 0.184320869221132 1103 3 0.19843472247640648 1103 4 0.04975958364509836 1103 5 0.028072092575726515 1103 6 0.04039426848392225 1103 7 0.07497880528108425 1103 8 0.08145128861109896 1103 9 0.16617174973116988 1103 10-11 0.14441594998591256 1103 12-13 0.14564024452205615 1103 14-15 0.2784679248255273 1103 16-17 0.32928015622530893 1103 18-19 0.3048415042087811 1103 20-21 0.25472698651923054 1103 22-23 0.2436261349558677 1103 24-25 0.33922135519240015 1103 26-27 0.26357022900044313 1103 28-29 0.3561478779446219 1103 30-31 0.32699444267289834 1103 32-33 0.2776453868709581 1103 34-35 0.40592296442714115 1103 36-37 0.36389632572561936 1103 38-39 0.41415897605053686 1103 40-41 0.3608372621913176 1103 42-43 0.5037108223976432 1103 44-45 0.6026168023666045 1103 46-47 0.6402003908549361 1103 48-49 0.6789471859513583 1103 50-51 0.7945595594393637 1103 52-53 0.8352540929672472 1103 54-55 0.8974975395622664 1103 56-57 0.9193924295936995 1103 58-59 1.179565597890658 1103 60-61 1.1627139431402966 1103 62-63 1.273287345071303 1103 64-65 1.2737017915117264 1103 66-67 1.3313849996292397 1103 68-69 1.5826793622144173 1103 70-71 1.5668982097590423 1103 72-73 1.7465793781950332 1103 74-75 1.8354886101339112 1103 76-77 1.9188893371377915 1103 78-79 2.031650613569461 1103 80-81 2.1807581065085486 1103 82-83 2.2436154867700253 1103 84-85 2.459222787965114 1103 86-87 2.722913783131606 1103 88-89 2.8703070337444814 1103 90-91 3.0537174220664554 1103 92-93 2.898063115331837 1103 94-95 3.0688647380883545 1103 96-97 3.2586541508423785 1103 98-99 3.628302662395374 1103 100-101 3.3809354735903945 1104 1 0.210133276035819 1104 2 0.19996009027653372 1104 3 0.1947451604152306 1104 4 0.05746536060737384 1104 5 0.0347796768086539 1104 6 0.07244219423839127 1104 7 0.15213686305644103 1104 8 0.1954356770018535 1104 9 0.14005671479181814 1104 10-11 0.16425398852678086 1104 12-13 0.2495753692883298 1104 14-15 0.35057692997225587 1104 16-17 0.3083999023634334 1104 18-19 0.26045657275116696 1104 20-21 0.26318284071641074 1104 22-23 0.2971566947096349 1104 24-25 0.30113478020733453 1104 26-27 0.3523907569693776 1104 28-29 0.2567715660583687 1104 30-31 0.21345952754793274 1104 32-33 0.33127636502168656 1104 34-35 0.2826434272218066 1104 36-37 0.2621842115940538 1104 38-39 0.4015080814541676 1104 40-41 0.5330682281511727 1104 42-43 0.6472261644010828 1104 44-45 0.7508228947807041 1104 46-47 0.8322684836451799 1104 48-49 1.0808832161763888 1104 50-51 1.058894253044734 1104 52-53 1.115891602611839 1104 54-55 1.2187237484773377 1104 56-57 1.2614813093828516 1104 58-59 1.2409453336404965 1104 60-61 1.4002038426906367 1104 62-63 1.3347840402510727 1104 64-65 1.6682718522941613 1104 66-67 1.7561117079011659 1104 68-69 1.839567986099464 1104 70-71 1.9727853327081526 1104 72-73 2.229529741029758 1104 74-75 2.025961730564614 1104 76-77 2.200383724387599 1104 78-79 2.410277983951584 1104 80-81 2.520012999398194 1104 82-83 2.746074109441196 1104 84-85 2.922567337942219 1104 86-87 3.0822378982905363 1104 88-89 3.068575409008684 1104 90-91 3.4438490157520008 1104 92-93 3.777242982263786 1104 94-95 4.002295155563186 1104 96-97 4.278381481682313 1104 98-99 4.600858223334367 1104 100-101 3.777408013652252 1105 1 0.09035937485659673 1105 2 0.12563039826825673 1105 3 0.15765562162020785 1105 4 0.09993862654813768 1105 5 0.0013683783647095993 1105 6 -0.017997334072688886 1105 7 0.08067475144229519 1105 8 0.06822851129940943 1105 9 0.1124204456586213 1105 10-11 0.08062385619281542 1105 12-13 0.1405305304900537 1105 14-15 0.26214116406841725 1105 16-17 0.38059146027763546 1105 18-19 0.25209451931553417 1105 20-21 0.24742000822030974 1105 22-23 0.20080185895317726 1105 24-25 0.20190498099702836 1105 26-27 0.25347621440899104 1105 28-29 0.2217684799394064 1105 30-31 0.2276401703466462 1105 32-33 0.3835838665056812 1105 34-35 0.27896887840058326 1105 36-37 0.32290027978989855 1105 38-39 0.3099788135288364 1105 40-41 0.4676653936468824 1105 42-43 0.34284402313657125 1105 44-45 0.37060048748978147 1105 46-47 0.3417675282772521 1105 48-49 0.4005409079956621 1105 50-51 0.4071074049319918 1105 52-53 0.557709400510916 1105 54-55 0.7455530491834494 1105 56-57 0.8185228287574802 1105 58-59 0.9635962655302919 1105 60-61 0.838602545634707 1105 62-63 0.8517281623019031 1105 64-65 1.0025295447040818 1105 66-67 1.2085020473151822 1105 68-69 1.252600710773347 1105 70-71 1.417415338053484 1105 72-73 1.397211578407532 1105 74-75 1.5561732109271524 1105 76-77 1.544946691550706 1105 78-79 1.6055665582236855 1105 80-81 1.791304267010485 1105 82-83 1.8747527189452597 1105 84-85 2.118081833841668 1105 86-87 2.2221557396020053 1105 88-89 2.18961845815328 1105 90-91 2.4971510153798953 1105 92-93 2.850079819597262 1105 94-95 3.001071044262087 1105 96-97 3.1927977917472 1105 98-99 3.2843132294918362 1105 100-101 2.9033268354340507 1106 1 0.14847559162327428 1106 2 0.12817982365056224 1106 3 0.1066310327209834 1106 4 0.08961464063311553 1106 5 0.0028123406180213806 1106 6 0.06771605639245593 1106 7 0.11202485021472341 1106 8 0.14051089268110672 1106 9 0.13827836053249598 1106 10-11 0.08046469513892163 1106 12-13 0.12153814221019843 1106 14-15 0.0352491889663753 1106 16-17 0.10030848830279382 1106 18-19 0.04319949344859708 1106 20-21 0.01934031897485511 1106 22-23 -0.016866015011210322 1106 24-25 -0.06585376075472738 1106 26-27 -0.1004680599946397 1106 28-29 -0.010979696176995901 1106 30-31 0.07457732297557129 1106 32-33 0.0574423339924337 1106 34-35 0.047753324721888646 1106 36-37 0.12723140694451018 1106 38-39 0.07527667139434158 1106 40-41 0.21777814756276825 1106 42-43 0.26831724538712365 1106 44-45 0.191329950728921 1106 46-47 0.33263078175232863 1106 48-49 0.2899404732165607 1106 50-51 0.46626368755644165 1106 52-53 0.40282314428749544 1106 54-55 0.4486385185507089 1106 56-57 0.5999110264846479 1106 58-59 0.6676063170077953 1106 60-61 0.6712363299362281 1106 62-63 0.6618500931996536 1106 64-65 0.8038559580328766 1106 66-67 0.9762154501239202 1106 68-69 0.8929534619926365 1106 70-71 0.8189812881749496 1106 72-73 1.3449103778038314 1106 74-75 1.491538274874049 1106 76-77 1.5052018857081997 1106 78-79 1.619719548433796 1106 80-81 1.3681433308538864 1106 82-83 2.0181372449301698 1106 84-85 2.0814563947541806 1106 86-87 2.1444027755982766 1106 88-89 2.0671042072451975 1106 90-91 2.3589257525208467 1106 92-93 2.5168233963033906 1106 94-95 2.813766494273647 1106 96-97 3.179837619278807 1106 98-99 3.2044196027229646 1106 100-101 3.132964783797032 1107 1 0.21951913515240307 1107 2 0.19291970083104815 1107 3 0.18947805351276514 1107 4 0.13067197688177146 1107 5 0.05839906390170313 1107 6 0.10614202586610588 1107 7 0.14185988468212685 1107 8 0.10348819084380523 1107 9 0.15767311258029082 1107 10-11 0.18438676579373237 1107 12-13 0.24904821079945805 1107 14-15 0.37422890685390797 1107 16-17 0.3127044910056682 1107 18-19 0.2684758997396983 1107 20-21 0.2522677493057799 1107 22-23 0.23243835009177616 1107 24-25 0.26871615019248907 1107 26-27 0.3871782416944711 1107 28-29 0.41675657973114966 1107 30-31 0.4477276156269312 1107 32-33 0.48711302201590456 1107 34-35 0.47901456670012266 1107 36-37 0.5630533887193039 1107 38-39 0.590916698691828 1107 40-41 0.5538197138474814 1107 42-43 0.524810957557925 1107 44-45 0.6224094253961994 1107 46-47 0.6800495419624966 1107 48-49 0.7854595190713525 1107 50-51 0.837228213296136 1107 52-53 0.8677974833023683 1107 54-55 0.9176279354939609 1107 56-57 0.8862743269086906 1107 58-59 1.141642506339501 1107 60-61 1.22805811110711 1107 62-63 1.1408394717498211 1107 64-65 1.1575164757253802 1107 66-67 1.499058382051043 1107 68-69 1.6680879818005927 1107 70-71 1.8258740485809177 1107 72-73 1.8322465352692738 1107 74-75 1.849569126605676 1107 76-77 1.8315026437798707 1107 78-79 1.6364991307937053 1107 80-81 1.915709256597804 1107 82-83 2.1013401217248067 1107 84-85 2.2846050604981336 1107 86-87 2.3769540735728505 1107 88-89 2.5586763229159146 1107 90-91 2.6254129474637082 1107 92-93 2.7349745240045884 1107 94-95 2.956567452949237 1107 96-97 3.295073175316407 1107 98-99 3.50438473699322 1107 100-101 3.318588321260055 1108 1 0.1801012774408619 1108 2 0.17575444474044843 1108 3 0.11015506955483545 1108 4 0.13198084970417767 1108 5 0.013735888906289517 1108 6 0.11787804221656017 1108 7 0.11053637868675992 1108 8 0.1568214200383906 1108 9 0.209014688327116 1108 10-11 0.22969833043467247 1108 12-13 0.21160701559576012 1108 14-15 0.3823505949899513 1108 16-17 0.32631084756732065 1108 18-19 0.31657351167037007 1108 20-21 0.27402993303473977 1108 22-23 0.29565703338838745 1108 24-25 0.27717255200409596 1108 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60-61 0.22995457024373422 2316 62-63 0.4285924740736249 2316 64-65 0.7370833984785321 2316 66-67 0.8903232203117817 2316 68-69 1.1030842646761805 2316 70-71 1.3099031131072962 2316 72-73 1.309077702833374 2316 74-75 1.6433825132527318 2316 76-77 1.7163211739272768 2316 78-79 1.7519623078788022 2316 80-81 1.6981464081677835 2316 82-83 2.0190310133992533 2316 84-85 2.1619800691660487 2316 86-87 2.300151913286573 2316 88-89 2.587116457054922 2316 90-91 2.836059606163957 2316 92-93 2.881154573931777 2316 94-95 3.338521136450204 2316 96-97 3.5718605598315634 2316 98-99 3.865884459619945 2316 100-101 4.131143957178605 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 4.0 4 22.0 5 31.0 6 93.0 7 243.0 8 537.0 9 1013.0 10 1833.0 11 3021.0 12 4554.0 13 6744.0 14 9042.0 15 12700.0 16 16951.0 17 23557.0 18 24325.0 19 20367.0 20 19568.0 21 21147.0 22 22693.0 23 25066.0 24 27915.0 25 31424.0 26 35921.0 27 41862.0 28 49899.0 29 59220.0 30 71194.0 31 84240.0 32 102311.0 33 127828.0 34 167128.0 35 228284.0 36 310117.0 37 365541.0 38 178942.0 39 7656.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.52332912029231 18.464912405836632 14.875506064211311 23.136252409659754 2 16.01725960699344 19.53566654127311 39.558085197551925 24.888988654181528 3 19.768180586134584 23.29017133237911 29.517482750257972 27.424165331228334 4 13.440493858392324 16.343462115855292 35.64652718964921 34.56951683610317 5 14.061715223713758 36.92639771408876 33.79993230244525 15.211954759752233 6 33.31610391290124 35.389578277045274 16.40629160738605 14.888026202667437 7 29.42970831110312 32.54464824911531 19.489499256773914 18.536144183007654 8 27.15848317136576 34.55727146975763 18.75945378800595 19.524791570870658 9 26.46640931928479 16.626295652769336 19.61902887026782 37.28826615767805 10-11 25.688137959499645 26.16676509776062 26.1469600008369 21.99813694190283 12-13 26.492063220342043 23.372510810777396 26.89456080236082 23.240865166519733 14-15 23.087536150840183 24.7386107616094 25.423420133390774 26.750432954159642 16-17 22.617373135634246 28.02321357074723 26.278914728239833 23.080498565378694 18-19 22.627953289453036 26.256232780502465 28.814775505778904 22.301038424265595 20-21 23.057032021964872 25.347789865306318 29.312851106565212 22.282327006163595 22-23 22.999196618713434 25.27719507471874 29.183853534009845 22.53975477255798 24-25 22.816328044496796 25.423315357280586 29.149807677950797 22.61054892027182 26-27 22.2195301265322 25.85095850230923 29.102523986334827 22.826987384823745 28-29 22.474957132545313 26.1486480703226 28.983748883734332 22.392645913397754 30-31 22.455199586874713 25.750430101084454 28.851152214414306 22.943218097626527 32-33 22.250776749719687 25.67216073845194 28.93296414540412 23.144098366424252 34-35 23.006791789550824 25.868697775470757 28.679860916096207 22.444649518882212 36-37 23.07409924121852 25.450587429154005 28.625110973634044 22.85020235599343 38-39 22.53800159260847 25.83758769986963 28.62441474967538 22.999995957846522 40-41 23.0684726949777 25.594946671596663 28.6290361891265 22.70754444429913 42-43 22.66500163705192 25.650855939193445 28.50034869086967 23.18379373288496 44-45 22.67080346604759 25.958788614275242 28.608659222393307 22.761748697283863 46-47 23.113190051897437 26.225490895361563 27.913703034816077 22.74761601792492 48-49 22.853417556112856 26.224159460274254 27.862228803315652 23.060194180297234 50-51 23.037559783812984 26.499124581430095 27.748842840255367 22.714472794501553 52-53 23.41944389760503 26.198339129831087 27.613559525134168 22.768657447429714 54-55 22.93116385496107 26.440993174493126 27.733269804859166 22.894573165686634 56-57 22.898829002840714 26.57190177432746 27.637834439850995 22.891434782980834 58-59 22.96523432782024 26.209727654952893 27.517505993835456 23.30753202339141 60-61 22.661738454793497 26.481126432124867 27.773379286864348 23.08375582621729 62-63 22.753750129577167 26.519714274030264 27.888500870663446 22.838034725729127 64-65 22.50660249149546 26.641207725747197 27.627634696057147 23.2245550867002 66-67 22.420497833326124 26.589056644506186 27.627742935901356 23.362702586266334 68-69 22.83342130415555 26.285158609014868 27.79671131780292 23.084708769026662 70-71 22.79711687242094 26.413698755203296 27.295251436761113 23.49393293561465 72-73 22.834254401106232 26.47030852204048 27.25499930099658 23.440437775856708 74-75 22.665780311308545 26.901360631556727 27.175755137675146 23.257103919459592 76-77 22.779285152501625 26.485144513013353 27.464391244102455 23.271179090382567 78-79 22.846926810062225 26.665839274862414 27.455689364781833 23.031544550293535 80-81 22.97165727964188 26.707525922002063 27.168464542058302 23.152352256297753 82-83 22.88692619580261 26.743295547515526 27.292333933839114 23.077444322842748 84-85 22.856456049181702 26.821868508951464 27.159236595628123 23.16243884623871 86-87 23.07092958921235 26.791868558223825 26.976159617789236 23.16104223477459 88-89 22.861929230421012 27.056116184829822 26.646961427374492 23.434993157374677 90-91 22.86213926947425 26.915279222516126 26.90263024935358 23.319951258656047 92-93 22.961406451944228 26.600075891799978 26.962250011174543 23.47626764508125 94-95 22.75639463460063 26.406762550621295 27.102651529831167 23.73419128494691 96-97 22.914798792672823 26.17586605753468 27.163292308460445 23.746042841332056 98-99 22.739437202388594 26.440755418227536 26.739377287809667 24.080430091574204 100-101 23.167493582958393 25.875418569905573 26.860823188273482 24.096264658862555 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1037.0 1 706.5 2 519.5 3 685.5 4 559.0 5 491.5 6 1012.5 7 2379.5 8 3808.5 9 4348.0 10 3530.0 11 2402.5 12 2185.0 13 2335.0 14 2447.0 15 2587.0 16 2766.5 17 2905.0 18 3113.0 19 3413.5 20 3805.0 21 4324.0 22 5200.0 23 6286.5 24 7639.0 25 9516.0 26 11869.0 27 14707.0 28 16939.0 29 18996.5 30 21467.5 31 24935.0 32 30591.0 33 33221.0 34 33545.0 35 35594.0 36 38799.5 37 42225.5 38 45143.5 39 47828.5 40 51224.0 41 55402.0 42 58324.0 43 61318.0 44 64345.5 45 68098.0 46 71020.0 47 74550.5 48 77296.0 49 76900.0 50 77183.5 51 77274.5 52 77459.5 53 79226.0 54 81508.0 55 78605.5 56 73027.5 57 68315.0 58 64204.5 59 57865.5 60 47544.5 61 39308.0 62 32833.5 63 25156.5 64 18322.5 65 13689.0 66 10057.0 67 7525.5 68 5704.0 69 4284.0 70 3232.0 71 2180.0 72 1478.5 73 1091.0 74 765.0 75 527.5 76 339.5 77 186.5 78 109.0 79 67.5 80 38.0 81 24.0 82 14.5 83 10.5 84 5.5 85 2.5 86 2.5 87 1.0 88 0.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.013361902126206733 3 0.003043280199563099 4 0.0020447038840814575 5 0.11768935146747922 6 9.510250623634684E-5 7 0.0 8 4.755125311817342E-5 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 3.5663439838630066E-4 24-25 5.230637842999076E-4 26-27 0.0061816629053625454 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011150768856211668 36-37 8.083713030089482E-4 38-39 0.007346668606757794 40-41 0.010532602565675413 42-43 0.006110336025685285 44-45 0.0038041002494538737 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.755125311817342E-5 68-69 0.001141230074836162 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.1887813279543355E-4 82-83 0.0021160307637587174 84-85 0.004660022805580996 86-87 0.003352363344831226 88-89 0.0 90-91 0.0026866458011767986 92-93 0.0 94-95 2.377562655908671E-5 96-97 7.132687967726013E-5 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2102994.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.97707657979236 #Duplication Level Percentage of deduplicated Percentage of total 1 75.1174679788947 39.79503853598004 2 13.963984852193246 14.79542189747404 3 4.614638201835631 7.334101242200446 4 2.0455460878709824 4.334682069785428 5 1.154410042621176 3.05786346162117 6 0.6737430534199984 2.1415762401680634 7 0.46590995712572314 1.7277783234556046 8 0.3164293641118805 1.3410802123720076 9 0.22961523982747367 1.0947909729804715 >10 1.2812197827250476 12.27559935744982 >50 0.08523737615435333 3.0728637793638636 >100 0.04533722384486159 4.512486735069271 >500 0.0037688229687698185 1.405329779828062 >1k 0.002422814765637729 1.9708939632599767 >5k 2.6920164062641433E-4 1.1404934289917772 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9924 0.47189863594475306 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8646 0.4111281344597274 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5421 0.2577753431536181 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2462 0.11707118517694295 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2373 0.11283912364942553 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2365 0.11245871362448015 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2321 0.11036645848728052 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2221 0.10561133317546319 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2165 0.10294846300084547 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.755125311817342E-5 0.0 9 0.0 0.0 0.0 4.755125311817342E-5 0.0 10-11 0.0 0.0 0.0 9.510250623634684E-5 4.755125311817342E-5 12-13 0.0 0.0 0.0 9.510250623634684E-5 1.6642938591360698E-4 14-15 0.0 0.0 0.0 9.510250623634684E-5 2.615318921499538E-4 16-17 0.0 0.0 0.0 9.510250623634684E-5 3.3285877182721396E-4 18-19 0.0 0.0 0.0 9.510250623634684E-5 3.3285877182721396E-4 20-21 0.0 0.0 0.0 1.1887813279543355E-4 3.8041002494538736E-4 22-23 0.0 0.0 0.0 3.5663439838630066E-4 3.8041002494538736E-4 24-25 0.0 0.0 0.0 6.181662905362545E-4 3.8041002494538736E-4 26-27 0.0 0.0 0.0 9.03473809245295E-4 3.8041002494538736E-4 28-29 0.0 0.0 0.0 0.0030432801995630993 3.8041002494538736E-4 30-31 0.0 0.0 0.0 0.011126993229652582 3.8041002494538736E-4 32-33 0.0 0.0 0.0 0.027888809953808713 3.8041002494538736E-4 34-35 0.0 0.0 0.0 0.052995871600204275 3.8041002494538736E-4 36-37 0.0 0.0 0.0 0.09217810416957918 3.8041002494538736E-4 38-39 0.0 0.0 0.0 0.15321013754675478 4.041856515044741E-4 40-41 0.0 0.0 0.0 0.21602534291586187 4.279612780635608E-4 42-43 0.0 0.0 0.0 0.2772713569320692 4.279612780635608E-4 44-45 0.0 0.0 0.0 0.35318693253523314 4.279612780635608E-4 46-47 0.0 0.0 0.0 0.44286859591610817 4.755125311817342E-4 48-49 0.0 0.0 0.0 0.539041005347614 5.468394108589943E-4 50-51 0.0 0.0 0.0 0.6402538476096461 6.181662905362545E-4 52-53 0.0 0.0 0.0 0.7344766556633067 6.181662905362545E-4 54-55 0.0 0.0 0.0 0.8427746346399467 6.894931702135146E-4 56-57 0.0 0.0 0.0 0.9616765430619394 7.132687967726013E-4 58-59 0.0 0.0 0.0 1.0833601997913451 7.132687967726013E-4 60-61 0.0 0.0 0.0 1.2151722734349217 7.608200498907747E-4 62-63 0.0 0.0 0.0 1.347103225211294 7.608200498907747E-4 64-65 0.0 0.0 0.0 1.484740327361847 7.608200498907747E-4 66-67 0.0 0.0 0.0 1.6412077257471966 7.845956764498615E-4 68-69 0.0 0.0 0.0 1.815150209653475 8.083713030089482E-4 70-71 0.0 0.0 0.0 1.9932534282075935 8.083713030089482E-4 72-73 0.0 0.0 0.0 2.178988622887179 8.321469295680349E-4 74-75 0.0 0.0 0.0 2.366554540811814 8.796981826862083E-4 76-77 0.0 0.0 0.0 2.55851894964988 9.03473809245295E-4 78-79 0.0 0.0 0.0 2.7793707447572364 9.03473809245295E-4 80-81 0.0 0.0 0.0 3.014250159534454 9.272494358043817E-4 82-83 0.0 0.0 0.0 3.2515071369675805 9.510250623634684E-4 84-85 0.0 0.0 0.0 3.494066079123383 9.748006889225551E-4 86-87 0.0 2.377562655908671E-5 0.0 3.744352099910889 9.985763154816418E-4 88-89 0.0 4.755125311817342E-5 0.0 4.012564943123946 9.985763154816418E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4740 0.0 52.972477 1 GTATCAA 8155 0.0 38.895252 1 ATCAACG 10210 0.0 31.106588 3 TATCAAC 10230 0.0 31.052425 2 TCAACGC 10335 0.0 30.729626 4 CAACGCA 10450 0.0 30.39001 5 AACGCAG 10830 0.0 29.382816 6 TGGTATC 2235 0.0 26.596666 2 GTGGTAT 2390 0.0 25.667673 1 ACGCAGA 12465 0.0 25.376345 7 CGCAGAG 12590 0.0 25.01122 8 GTAAGGT 1070 0.0 23.549595 4 GAGTACT 8420 0.0 22.70392 12-13 GCAGAGT 13965 0.0 22.412565 9 TACATGG 7525 0.0 22.308117 2 GTACATG 7690 0.0 22.138662 1 TAAGGTG 1200 0.0 21.393566 5 AGGTAAG 1095 0.0 21.280247 2 GTACTTT 8975 0.0 21.14119 14-15 CGCGCGT 135 3.8001384E-4 21.10885 7 >>END_MODULE