FastQCFastQC Report
Thu 26 May 2016
SRR938122_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938122_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085861
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102720.49245850993906115No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85810.4113888701116709No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52280.2506399036177387No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24920.11947104816668033No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22590.108300601046762No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22070.10580762572386176No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA21800.10451319622927895No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA20860.10000666391480545No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA44850.053.6407361
GTATCAA78200.039.2492031
TATCAAC99800.030.4823172
ATCAACG100050.030.3118613
TCAACGC101400.029.9082974
CAACGCA103250.029.418455
AACGCAG107350.028.4249866
ACGCAGA119750.025.2704127
CGCAGAG121100.024.8317898
GTACATG78150.023.5157261
TACATGG78150.023.1128352
TGGTATC21400.023.1003272
AGGTAAG10150.022.947142
GTGGTAT22700.022.9206681
TAAGGTA5650.022.7156034
GCAGAGT130750.022.598879
GAGTACT80800.021.89860512-13
ACATGGG78950.021.373973
GTACTTT83950.021.08020414-15
AGAGTAC117300.021.077510-11
Produced by FastQC (version 0.11.5)