##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938122_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2085861 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.787968613440686 33.0 31.0 34.0 30.0 34.0 2 31.97628509282258 33.0 31.0 34.0 30.0 34.0 3 32.04997552569419 34.0 31.0 34.0 30.0 34.0 4 35.57866272009496 37.0 35.0 37.0 33.0 37.0 5 35.579748123197085 37.0 35.0 37.0 33.0 37.0 6 35.604142845568326 37.0 35.0 37.0 33.0 37.0 7 35.543536218376964 37.0 35.0 37.0 33.0 37.0 8 35.51367996237525 37.0 35.0 37.0 33.0 37.0 9 37.13501283163164 39.0 37.0 39.0 34.0 39.0 10-11 37.04441427305079 39.0 37.0 39.0 33.0 39.0 12-13 36.93523178198356 39.0 37.0 39.0 33.0 39.0 14-15 38.0979605064767 40.0 38.0 41.0 33.0 41.0 16-17 37.939724411166424 40.0 38.0 41.0 33.0 41.0 18-19 38.08635882256775 40.0 38.0 41.0 33.0 41.0 20-21 38.06208299594268 40.0 38.0 41.0 33.0 41.0 22-23 37.97143289989122 40.0 38.0 41.0 33.0 41.0 24-25 37.8575302956429 40.0 38.0 41.0 33.0 41.0 26-27 37.666737380870536 40.0 38.0 41.0 32.0 41.0 28-29 37.45752545351776 40.0 37.0 41.0 32.0 41.0 30-31 37.23014596849934 40.0 37.0 41.0 31.0 41.0 32-33 36.96244692239799 40.0 36.0 41.0 30.5 41.0 34-35 36.71380859031355 39.0 36.0 41.0 30.0 41.0 36-37 36.43718589110205 39.0 35.5 41.0 30.0 41.0 38-39 36.12602349821009 39.0 35.0 40.5 29.5 41.0 40-41 35.903742147727 39.0 35.0 40.0 29.0 41.0 42-43 35.71810753449056 39.0 35.0 40.0 27.5 41.0 44-45 35.43113227583238 39.0 35.0 40.0 26.5 41.0 46-47 35.02738125886624 38.0 34.5 40.0 25.0 41.0 48-49 34.605197326188076 38.0 34.0 40.0 23.5 41.0 50-51 33.88664513119522 37.5 33.5 39.5 23.0 40.5 52-53 34.00398204866 38.0 33.5 39.5 23.0 40.5 54-55 34.09001630501744 38.0 34.0 40.0 22.5 41.0 56-57 33.75948756892238 38.0 33.0 40.0 20.5 41.0 58-59 33.40627060000642 37.0 33.0 40.0 19.5 41.0 60-61 32.95631923699614 37.0 32.5 40.0 17.0 41.0 62-63 32.388951133368906 36.0 31.5 39.0 12.0 41.0 64-65 31.83578723606223 35.5 31.0 39.0 7.5 40.0 66-67 31.271069356970575 35.0 30.5 38.5 4.5 40.0 68-69 30.63957090141673 35.0 29.5 38.0 2.0 40.0 70-71 30.067588396350477 34.5 29.0 37.0 2.0 39.5 72-73 29.409415344550762 34.0 28.5 36.5 2.0 39.0 74-75 28.699309302010057 34.0 27.5 36.0 2.0 39.0 76-77 28.022429346921967 33.5 26.0 35.0 2.0 37.5 78-79 27.28233568775676 33.0 25.5 35.0 2.0 37.0 80-81 26.617754011413034 32.5 24.5 35.0 2.0 36.5 82-83 25.87656128572326 32.0 21.0 35.0 2.0 36.0 84-85 25.204857610358506 31.5 19.0 34.5 2.0 35.5 86-87 24.505214633189844 31.0 15.0 34.0 2.0 35.0 88-89 23.659429367536955 31.0 6.5 34.0 2.0 35.0 90-91 22.806608637871843 30.0 2.0 34.0 2.0 35.0 92-93 21.890437090486856 29.0 2.0 34.0 2.0 35.0 94-95 20.91997836864489 29.0 2.0 34.0 2.0 35.0 96-97 19.700210848182117 27.0 2.0 33.5 2.0 35.0 98-99 18.199721601774996 24.5 2.0 33.0 2.0 35.0 100-101 14.669772818035334 2.0 2.0 29.0 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.45290762827593767 1101 2 0.3777889821318823 1101 3 0.38833687825439966 1101 4 0.31128040408822955 1101 5 0.2969780051354931 1101 6 0.4291411852398639 1101 7 0.3551847376956516 1101 8 0.3073942521677253 1101 9 0.44051972600187383 1101 10-11 0.4815390256402807 1101 12-13 0.4402856738630234 1101 14-15 0.6820676494647913 1101 16-17 0.759780768319871 1101 18-19 0.6567525852737859 1101 20-21 0.6630802717378756 1101 22-23 0.7436789242524853 1101 24-25 0.6399937734347461 1101 26-27 0.7952163113275006 1101 28-29 0.6739211110044678 1101 30-31 0.7024193784714328 1101 32-33 0.6609471249284482 1101 34-35 0.7181691385326658 1101 36-37 0.864800518708499 1101 38-39 0.9873165286323413 1101 40-41 1.048663415050342 1101 42-43 1.1215255027665307 1101 44-45 1.1121081987700876 1101 46-47 1.0324485060462507 1101 48-49 1.0927123941894479 1101 50-51 0.8788315415274184 1101 52-53 0.9483776607969432 1101 54-55 1.0518128651933267 1101 56-57 1.0894898681467637 1101 58-59 1.1196145924706116 1101 60-61 1.1143130162842212 1101 62-63 1.1375144218504687 1101 64-65 1.129110295148319 1101 66-67 1.2084550308307236 1101 68-69 1.4623769912009053 1101 70-71 1.6999043038206985 1101 72-73 1.7273513075502294 1101 74-75 1.909767633195134 1101 76-77 2.1815757466182113 1101 78-79 2.2008954355975554 1101 80-81 2.3978510808413454 1101 82-83 2.5103200507244594 1101 84-85 2.461667162309187 1101 86-87 2.7489571216815527 1101 88-89 3.256907591868231 1101 90-91 3.399667240244437 1101 92-93 3.834573746769813 1101 94-95 3.916128983824798 1101 96-97 3.9618970387077006 1101 98-99 4.077234412258569 1101 100-101 3.835904353578096 1102 1 0.43383178559168556 1102 2 0.2718596074185129 1102 3 0.2510243059723791 1102 4 0.17737022317610496 1102 5 0.2787737595089723 1102 6 0.27575850979984295 1102 7 0.2294523252513585 1102 8 0.31351643078704683 1102 9 0.5540267064261997 1102 10-11 0.46942247844012996 1102 12-13 0.44962073241583056 1102 14-15 0.657233225684557 1102 16-17 0.5886140908079298 1102 18-19 0.5963829524204414 1102 20-21 0.6541597532491039 1102 22-23 0.7395359864830127 1102 24-25 0.6851938065149881 1102 26-27 0.7551452717600498 1102 28-29 0.7682563264032751 1102 30-31 0.5998226569933607 1102 32-33 0.7325478380721719 1102 34-35 0.768550144664701 1102 36-37 0.8573905178451966 1102 38-39 0.8862662308453295 1102 40-41 1.0042407418087578 1102 42-43 1.105563895553992 1102 44-45 0.9693178126774811 1102 46-47 0.9718337527235121 1102 48-49 1.2002611713481102 1102 50-51 1.2854555495413393 1102 52-53 1.220672929466339 1102 54-55 1.0850131430051633 1102 56-57 1.164903105481713 1102 58-59 1.2187933676045333 1102 60-61 1.2190025299242464 1102 62-63 1.402659716976288 1102 64-65 1.5070285055381625 1102 66-67 1.4189406649046283 1102 68-69 1.5164165272789774 1102 70-71 1.6040011470164437 1102 72-73 1.889870585996455 1102 74-75 1.7759379492748018 1102 76-77 1.915171665200365 1102 78-79 2.3832859344118766 1102 80-81 2.3595711965599797 1102 82-83 2.695269472429402 1102 84-85 2.6316545135226157 1102 86-87 2.851935977027292 1102 88-89 3.3029991842231645 1102 90-91 3.3794525141947567 1102 92-93 3.626768882884914 1102 94-95 3.8799771496470434 1102 96-97 3.8338803782932693 1102 98-99 4.049513633379529 1102 100-101 3.677512328302301 1103 1 0.49078702427483023 1103 2 0.42385164769257955 1103 3 0.3907227361375405 1103 4 0.3908744068888268 1103 5 0.368286168783051 1103 6 0.4400380894220248 1103 7 0.3892043953807871 1103 8 0.4593444122654802 1103 9 0.4955371628323846 1103 10-11 0.4914425656290362 1103 12-13 0.49203426241625436 1103 14-15 0.8125696161252236 1103 16-17 0.755388114086422 1103 18-19 0.6820471383825364 1103 20-21 0.5880015607254663 1103 22-23 0.8126413659439038 1103 24-25 0.9316526358933785 1103 26-27 0.9569306505563517 1103 28-29 0.946351519766587 1103 30-31 0.9972924597511437 1103 32-33 1.135073945538693 1103 34-35 1.00323010054624 1103 36-37 1.0638914399618145 1103 38-39 1.3823432413215428 1103 40-41 1.46503017715132 1103 42-43 1.4958922470293174 1103 44-45 1.4981845997369234 1103 46-47 1.6366034664008708 1103 48-49 1.8463223978462722 1103 50-51 1.800727027573494 1103 52-53 1.9099742679732472 1103 54-55 1.8750408640160074 1103 56-57 1.8457521876465819 1103 58-59 2.0630809095087628 1103 60-61 2.0981649949854173 1103 62-63 2.0493692368238428 1103 64-65 2.3017516405045875 1103 66-67 2.4250719735433854 1103 68-69 2.646319944669983 1103 70-71 2.579967005057771 1103 72-73 2.572726533872263 1103 74-75 2.801711683712888 1103 76-77 3.004442522097836 1103 78-79 3.143172168354713 1103 80-81 3.159306543341007 1103 82-83 3.1757792949203605 1103 84-85 3.073620756258471 1103 86-87 3.2417839396160666 1103 88-89 3.5917616308803524 1103 90-91 4.004007271818033 1103 92-93 4.260588739768323 1103 94-95 4.2684075895918845 1103 96-97 4.835216904207751 1103 98-99 4.836557096192607 1103 100-101 4.62656762206961 1104 1 0.393008330966218 1104 2 0.36134476641285573 1104 3 0.2517043114740858 1104 4 0.350933979533238 1104 5 0.23862332485224869 1104 6 0.2773158018893085 1104 7 0.20451469522108567 1104 8 0.24592055976696514 1104 9 0.336278008806552 1104 10-11 0.3977004458042046 1104 12-13 0.3643993982127611 1104 14-15 0.5526428151833258 1104 16-17 0.4760993158035163 1104 18-19 0.4246416975248408 1104 20-21 0.5758475929055891 1104 22-23 0.5417930121827865 1104 24-25 0.5070548873889109 1104 26-27 0.6570409286133341 1104 28-29 0.6300930831696618 1104 30-31 0.5984062799653529 1104 32-33 0.5370808226748522 1104 34-35 0.573894775933347 1104 36-37 0.5590732122953384 1104 38-39 0.6011525461139868 1104 40-41 0.6378564030845979 1104 42-43 0.6725494959617606 1104 44-45 0.7848992607399197 1104 46-47 0.6598166126344296 1104 48-49 0.8864871697192669 1104 50-51 0.908240141960782 1104 52-53 0.9116390937126013 1104 54-55 1.0857554161024083 1104 56-57 1.2278299907987744 1104 58-59 1.1379237795558694 1104 60-61 1.092865965645423 1104 62-63 1.2590316190429718 1104 64-65 1.4241314267676053 1104 66-67 1.42925889928949 1104 68-69 1.4527934974702674 1104 70-71 1.6026687168242013 1104 72-73 1.8537057592147903 1104 74-75 2.0018792317752983 1104 76-77 1.8938856623054683 1104 78-79 2.2050549989550667 1104 80-81 2.162221098268713 1104 82-83 2.4162504243263 1104 84-85 2.51663650891361 1104 86-87 2.6896569878483625 1104 88-89 2.9835772587549485 1104 90-91 3.1684565385843833 1104 92-93 3.552397767533769 1104 94-95 3.6179918419090242 1104 96-97 3.577870953123142 1104 98-99 3.80326709272385 1104 100-101 3.7568628038123446 1105 1 0.24265060605887356 1105 2 0.14005936956962017 1105 3 0.08767008844112922 1105 4 0.05171461029122071 1105 5 0.17913161036126724 1105 6 0.14775392241286056 1105 7 0.17449761234603045 1105 8 0.11300025605501673 1105 9 0.1540927157016938 1105 10-11 0.2135643894812702 1105 12-13 0.15562249344397827 1105 14-15 0.33090121683116536 1105 16-17 0.28252992359411166 1105 18-19 0.23069367433657106 1105 20-21 0.26150001747829066 1105 22-23 0.2564720036313659 1105 24-25 0.18693714942801876 1105 26-27 0.23356073057956195 1105 28-29 0.41751435450617436 1105 30-31 0.23444784822232378 1105 32-33 0.3601593680983086 1105 34-35 0.4221653002819181 1105 36-37 0.5210179781722886 1105 38-39 0.6460612179350989 1105 40-41 0.6487783337163648 1105 42-43 0.7074349703972231 1105 44-45 0.6786760212166669 1105 46-47 0.9292301295216987 1105 48-49 0.9213250169341833 1105 50-51 0.9740824418326213 1105 52-53 0.9279432257374296 1105 54-55 1.151858309559124 1105 56-57 1.0219714738041006 1105 58-59 1.0517986846540168 1105 60-61 1.1169015469763295 1105 62-63 1.370749455860178 1105 64-65 1.3760481131975801 1105 66-67 1.6128273346148667 1105 68-69 1.6987097616561755 1105 70-71 1.7407004302606524 1105 72-73 1.6974888012771103 1105 74-75 1.886754475580016 1105 76-77 2.0201812257702016 1105 78-79 2.177986898105136 1105 80-81 2.325200579073428 1105 82-83 2.3603413834213534 1105 84-85 2.5732510619261753 1105 86-87 2.8640449780642747 1105 88-89 2.847860076350937 1105 90-91 2.8020272413560257 1105 92-93 3.095339402599034 1105 94-95 3.4307275776649604 1105 96-97 3.693602820767641 1105 98-99 3.7647014119659623 1105 100-101 3.663987336951669 1106 1 0.11010653306070495 1106 2 0.0681170399632407 1106 3 0.021878162952141622 1106 4 0.06692824105699913 1106 5 0.1125180977013116 1106 6 -0.04969894440036171 1106 7 0.08479681872007916 1106 8 0.19125590169837636 1106 9 0.1514171269987088 1106 10-11 0.2040320190958056 1106 12-13 0.26155367987505684 1106 14-15 0.34132108450409504 1106 16-17 0.22616868163882486 1106 18-19 0.3361005612303387 1106 20-21 0.1722516504839362 1106 22-23 0.15370961375396774 1106 24-25 0.19268421344644793 1106 26-27 0.20528188329745234 1106 28-29 0.22404026527431142 1106 30-31 0.16815206704476537 1106 32-33 0.16846453257652882 1106 34-35 0.17617724587827865 1106 36-37 0.11962142905137085 1106 38-39 -0.13929987899663843 1106 40-41 0.29858180657257805 1106 42-43 0.29345682955656827 1106 44-45 0.429931561020517 1106 46-47 0.470978172289378 1106 48-49 0.38203406923615546 1106 50-51 0.38539628067844234 1106 52-53 0.5181123112734269 1106 54-55 -0.0318752440566854 1106 56-57 0.36902795355648976 1106 58-59 0.7406144512908881 1106 60-61 0.7521382452180987 1106 62-63 0.4424529544628655 1106 64-65 0.6705609203105034 1106 66-67 0.7428513838215061 1106 68-69 0.3822818940496937 1106 70-71 0.5901497986202635 1106 72-73 0.9005039921215037 1106 74-75 1.1065379879993351 1106 76-77 1.4260894767584809 1106 78-79 1.5790536836868547 1106 80-81 1.7141624766373944 1106 82-83 1.7003752946554407 1106 84-85 1.9497998165657044 1106 86-87 1.9480846357235109 1106 88-89 2.1239091066629925 1106 90-91 2.275405517083488 1106 92-93 2.653381831677514 1106 94-95 2.7178452484120896 1106 96-97 2.8877761535132116 1106 98-99 3.442825897542292 1106 100-101 3.4218481565072327 1107 1 0.18752887891142223 1107 2 0.17188463771564955 1107 3 -0.005482717201033438 1107 4 0.11838321911591265 1107 5 0.16820020129622293 1107 6 0.10095092309173026 1107 7 0.24461922768262667 1107 8 0.09036871263229784 1107 9 0.11018510470582754 1107 10-11 0.09482392546005514 1107 12-13 0.12963765007253159 1107 14-15 0.25192643828334127 1107 16-17 0.2958040265786792 1107 18-19 0.2569222448624515 1107 20-21 0.29960946615228323 1107 22-23 0.23860298728174456 1107 24-25 0.2830385817764025 1107 26-27 0.3137939454617751 1107 28-29 0.4745278393059422 1107 30-31 0.5761182367886519 1107 32-33 0.592966807438863 1107 34-35 0.5230801387726771 1107 36-37 0.5026920759868929 1107 38-39 0.4321148342162431 1107 40-41 0.5368797709885271 1107 42-43 0.68262373071844 1107 44-45 0.4255851690476433 1107 46-47 0.4564044762949635 1107 48-49 0.6212476520268311 1107 50-51 0.41873083566451186 1107 52-53 0.41479734621373154 1107 54-55 0.5631451130262022 1107 56-57 0.6133236117405332 1107 58-59 0.6217609951628233 1107 60-61 0.7073940748310861 1107 62-63 0.8005798336598389 1107 64-65 0.9848829558407282 1107 66-67 1.0340949496974794 1107 68-69 1.1664668953427793 1107 70-71 1.2259558606268008 1107 72-73 1.5130083740242277 1107 74-75 1.6618655017103166 1107 76-77 1.7594234312711734 1107 78-79 1.829923800673562 1107 80-81 2.0353517535080066 1107 82-83 2.0352164373521404 1107 84-85 2.2828006118823474 1107 86-87 2.4110758048306877 1107 88-89 2.6270217328995784 1107 90-91 2.936244071946984 1107 92-93 3.033431269272075 1107 94-95 3.3772797308651192 1107 96-97 3.842875342397914 1107 98-99 4.027959563951999 1107 100-101 3.697154848286109 1108 1 0.2169277380601713 1108 2 0.14910543595876646 1108 3 0.13210064338921512 1108 4 0.09938438301387009 1108 5 0.09045028904421315 1108 6 0.1631202023392504 1108 7 0.11963649801560905 1108 8 0.1550068900923378 1108 9 0.18578791036818387 1108 10-11 0.1728723082611765 1108 12-13 0.19065224220556587 1108 14-15 0.3283668428471387 1108 16-17 0.43319386145176964 1108 18-19 0.35816206369493386 1108 20-21 0.29017246626927573 1108 22-23 0.18530404501982645 1108 24-25 0.26169725160114155 1108 26-27 0.27267311113957504 1108 28-29 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-1.019370375312839 2316 50-51 -1.392140447668524 2316 52-53 -1.5203655880092057 2316 54-55 -1.3868119710314417 2316 56-57 -1.2044757871529939 2316 58-59 -1.1676899923759763 2316 60-61 -1.1824355550153705 2316 62-63 -1.396880902265952 2316 64-65 -1.2770101423881535 2316 66-67 -1.327443962173291 2316 68-69 -1.4712149072589895 2316 70-71 -1.6959868860123954 2316 72-73 -1.723016656026374 2316 74-75 -1.8241912077652778 2316 76-77 -1.9517910851761613 2316 78-79 -2.7670236435852864 2316 80-81 -2.8312111091411474 2316 82-83 -2.431819114422236 2316 84-85 -2.4606293775326655 2316 86-87 -2.3997906628520482 2316 88-89 -2.5644083924867367 2316 90-91 -2.7642405146672075 2316 92-93 -2.9629509498362374 2316 94-95 -3.1410442906372325 2316 96-97 -3.4815006295844455 2316 98-99 -3.51188059422795 2316 100-101 -3.2545472952448193 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6189.0 3 2028.0 4 2162.0 5 2452.0 6 2808.0 7 3433.0 8 4358.0 9 5482.0 10 6811.0 11 8353.0 12 10378.0 13 12247.0 14 14451.0 15 17312.0 16 20771.0 17 25363.0 18 25216.0 19 21760.0 20 22041.0 21 23614.0 22 25769.0 23 28442.0 24 31900.0 25 35228.0 26 40130.0 27 46039.0 28 53226.0 29 61436.0 30 71469.0 31 85718.0 32 103803.0 33 129733.0 34 168565.0 35 225968.0 36 288297.0 37 300847.0 38 144137.0 39 7925.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.3280246080583 17.97877376777903 14.49696661470155 23.19623500946112 2 15.751939471696302 19.219846760752528 40.02167891701339 25.006534850537776 3 19.65109993030249 23.268223164420405 29.194957795352188 27.885719109924917 4 13.123835244511708 16.061189687551717 35.92059155787621 34.894383510060365 5 14.137078269982064 36.81283089918209 33.665180169416544 15.384910661419301 6 33.270316009487146 35.20723502854733 16.566374755707905 14.956074206257618 7 29.37274084249787 32.47320667981551 19.587231299356116 18.566821178330507 8 27.188683923887382 34.278066325397624 18.895395266007117 19.63785448470788 9 26.534123476212972 16.633530869053242 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34-35 23.00959324635457 25.86008250191865 28.616509976976207 22.513814274750576 36-37 23.016870548809045 25.444822319284977 28.626765097986457 22.911542033919524 38-39 22.56458863439529 25.8180699514788 28.5660541492389 23.051287264887012 40-41 23.047333560955398 25.631147713932773 28.606938572732922 22.714580152378907 42-43 22.661430723537933 25.67217794597928 28.46600501263709 23.20038631784569 44-45 22.623212655005524 25.93623061374552 28.585101009071796 22.85545572217716 46-47 23.121267532078903 26.221856478530864 27.884223898440908 22.77265209094933 48-49 22.862500863200054 26.231522723261897 27.836811070444796 23.06916534309325 50-51 23.008220055631504 26.494371398061578 27.732941934079403 22.76446661222752 52-53 23.327088705679046 26.208256334373196 27.630511439320344 22.83414352062741 54-55 22.840963170834915 26.457492960346585 27.798653325389623 22.902890543428878 56-57 22.87687062761025 26.580941055750817 27.662153025849545 22.88003529078939 58-59 22.937194745962472 26.195675210183555 27.552218638848693 23.31491140500528 60-61 22.588167148485805 26.48375760429518 27.828855348288347 23.099219898930663 62-63 22.7149179369259 26.49036837457885 27.961574673117877 22.83313901537737 64-65 22.461252455661434 26.556315143657095 27.772961423398947 23.209470977282525 66-67 22.36964620481668 26.494987222331435 27.783470059980726 23.351896512871164 68-69 22.778840893387912 26.151125653232732 27.953110608082415 23.11692284529694 70-71 22.70938247143771 26.34180188180731 27.464010027610453 23.484805619144527 72-73 22.788750230460266 26.404357131537488 27.389636204834282 23.41725643316796 74-75 22.56869666159769 26.793837732584024 27.419711746969107 23.21775385884918 76-77 22.837903958731438 26.4067819445733 27.388391802960747 23.36692229373451 78-79 22.919728398696368 26.542213902568957 27.34830551262804 23.189752186106638 80-81 23.033166265279775 26.5244530329385 27.119256860838824 23.323123840942902 82-83 22.899534243429073 26.656897774020678 27.17288134943059 23.270686633119663 84-85 22.9237854880212 26.655959815504605 27.108016209395895 23.3122384870783 86-87 23.14339308294829 26.641088797396666 26.84218790265068 23.37333021700437 88-89 23.019371737917453 26.79655970556704 26.609529842892517 23.574538713622992 90-91 22.97164246388766 26.68939074219724 26.691045291488425 23.647921502426673 92-93 23.13104429439707 26.264409386860976 26.898624999070964 23.705921319670995 94-95 22.987226308002562 26.098472768080878 27.021765872397363 23.892535051519197 96-97 23.16640634778443 25.87110115126965 26.953626473015635 24.00886602793029 98-99 23.006854795299635 26.06639673317657 26.595258253076192 24.331490218447595 100-101 23.837418392055202 25.014584636747717 26.62026311404922 24.527733857147865 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1070.0 1 709.0 2 499.0 3 669.5 4 564.0 5 512.5 6 978.5 7 2231.5 8 3776.0 9 4415.5 10 3517.5 11 2419.5 12 2190.5 13 2299.0 14 2468.5 15 2587.0 16 2773.0 17 2977.5 18 3136.5 19 3449.5 20 3842.0 21 4349.0 22 5142.0 23 6283.5 24 7704.0 25 9452.0 26 11690.0 27 14534.5 28 16836.0 29 18791.5 30 21095.0 31 24776.5 32 30208.5 33 32809.5 34 33327.0 35 34981.5 36 38224.5 37 42000.5 38 45004.0 39 47577.0 40 50438.0 41 54419.5 42 57280.5 43 59769.0 44 63080.5 45 66959.0 46 69666.0 47 72937.5 48 75707.0 49 75791.5 50 75717.0 51 75627.0 52 76278.5 53 77803.5 54 79905.0 55 77487.0 56 72317.5 57 68020.0 58 64199.5 59 58133.0 60 48473.5 61 40359.0 62 33611.0 63 25875.5 64 19071.5 65 14630.0 66 10902.5 67 8011.0 68 6094.5 69 4587.0 70 3431.0 71 2460.0 72 1735.5 73 1236.0 74 837.5 75 569.0 76 398.0 77 248.5 78 153.5 79 101.5 80 64.0 81 38.0 82 25.0 83 16.5 84 12.0 85 9.0 86 6.0 87 5.5 88 6.5 89 5.5 90 3.0 91 3.0 92 3.0 93 2.0 94 1.5 95 0.5 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4539132761003729 2 0.04247646415556933 3 0.05455780610500892 4 0.057338432426705326 5 0.08471321914547518 6 0.08524057930993484 7 0.04856507696342182 8 0.04305176615316169 9 0.048229484131492945 10-11 0.05127379053541919 12-13 0.047845949466431364 14-15 0.04029511074803163 16-17 0.040654674496526856 18-19 0.048469193297156425 20-21 0.05633165393091869 22-23 0.048684931546253564 24-25 0.06740621738457164 26-27 0.05791373442429769 28-29 0.04564062514232732 30-31 0.04794183313269676 32-33 0.046455636305583164 34-35 0.050866284953791266 36-37 0.045808421558291755 38-39 0.028141856048892997 40-41 0.025552997059727373 42-43 0.025481084310028327 44-45 0.015844775850356277 46-47 0.029340401877210417 48-49 0.02862127438021997 50-51 0.01687552526270926 52-53 0.016060514099453416 54-55 0.016947438012408306 56-57 0.01586874676692263 58-59 0.014526375439207118 60-61 0.017354943594036228 62-63 0.01733097267746988 64-65 0.01793024559162859 66-67 0.009156890128345082 68-69 0.013040178612093518 70-71 0.020087628082599944 72-73 0.01728303084433718 74-75 0.014262695356977287 76-77 0.009852046708769185 78-79 0.014574317272339815 80-81 0.018385693006389207 82-83 0.015796834017223583 84-85 0.013447684193721441 86-87 0.0035237247352532123 88-89 0.030323209456430703 90-91 0.03360722502602043 92-93 0.017810391008796847 94-95 0.03432635252301088 96-97 0.028669216213352663 98-99 0.025529026143161028 100-101 0.15293444769330267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2085861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.99588189642133 #Duplication Level Percentage of deduplicated Percentage of total 1 76.72707445981817 42.196731252500854 2 13.084395332259879 14.391757207581016 3 4.314904267218955 7.11905896423014 4 1.929630552151013 4.244869357992935 5 1.0562687248970235 2.9045215022660127 6 0.6439650327205888 2.1249254930955956 7 0.43237548700455974 1.6645209852747294 8 0.2883288374064906 1.268551895147186 9 0.22447564847505194 1.111071262694089 >10 1.1764431044034123 11.621507514462168 >50 0.07498222134199008 2.82441462830434 >100 0.04127517476772026 4.2069189482452956 >500 0.0035989172977139118 1.4442295347456275 >1k 0.0020189048255468225 1.7144221578686396 >5k 1.7555694135189758E-4 0.6666231789718997 >10k+ 8.777847067594879E-5 0.4958761166195491 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10272 0.49245850993906115 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8581 0.4113888701116709 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5228 0.2506399036177387 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2492 0.11947104816668033 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2259 0.108300601046762 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2207 0.10580762572386176 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2180 0.10451319622927895 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2086 0.10000666391480545 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 4.7941833132696764E-5 2 0.0 0.0 0.0 0.0 4.7941833132696764E-5 3 0.0 0.0 0.0 0.0 4.7941833132696764E-5 4 0.0 0.0 0.0 0.0 4.7941833132696764E-5 5 0.0 0.0 0.0 0.0 9.588366626539353E-5 6 0.0 0.0 0.0 0.0 1.4382549939809028E-4 7 0.0 0.0 0.0 0.0 1.4382549939809028E-4 8 0.0 0.0 0.0 0.0 1.4382549939809028E-4 9 0.0 0.0 0.0 0.0 1.4382549939809028E-4 10-11 0.0 0.0 0.0 0.0 1.4382549939809028E-4 12-13 0.0 0.0 0.0 4.7941833132696764E-5 3.3559283192887736E-4 14-15 0.0 0.0 0.0 4.7941833132696764E-5 4.075055816279225E-4 16-17 0.0 0.0 0.0 4.7941833132696764E-5 4.794183313269676E-4 18-19 0.0 0.0 0.0 1.4382549939809028E-4 4.794183313269676E-4 20-21 0.0 0.0 0.0 1.4382549939809028E-4 4.794183313269676E-4 22-23 0.0 0.0 0.0 2.397091656634838E-4 5.273601644596644E-4 24-25 0.0 0.0 0.0 5.273601644596644E-4 5.273601644596644E-4 26-27 0.0 0.0 0.0 0.0012704585780164643 5.753019975923611E-4 28-29 0.0 0.0 0.0 0.00326004465302338 5.753019975923611E-4 30-31 0.0 0.0 0.0 0.010762941538290422 5.753019975923611E-4 32-33 0.0 0.0 0.0 0.02706316480340732 5.753019975923611E-4 34-35 0.0 0.0 0.0 0.05223262719807312 5.753019975923611E-4 36-37 0.0 0.0 0.0 0.09068197737049594 5.753019975923611E-4 38-39 0.0 0.0 0.0 0.14919498470895232 5.753019975923611E-4 40-41 0.0 0.0 0.0 0.21020096737030897 5.753019975923611E-4 42-43 0.0 0.0 0.0 0.27334036160607056 5.753019975923611E-4 44-45 0.0 0.0 0.0 0.3511978986135701 5.753019975923611E-4 46-47 0.0 0.0 0.0 0.4405135337397842 5.992729141587095E-4 48-49 0.0 0.0 0.0 0.5335686318503485 6.232438307250579E-4 50-51 0.0 0.0 0.0 0.6336472085148531 6.232438307250579E-4 52-53 0.0 0.0 0.0 0.7273255504561427 6.232438307250579E-4 54-55 0.0 0.0 0.0 0.8310716773552984 6.232438307250579E-4 56-57 0.0 0.0 0.0 0.9495838888593248 6.472147472914063E-4 58-59 0.0 0.0 0.0 1.0779721179886868 6.711856638577547E-4 60-61 0.0 0.0 0.0 1.2135276511713866 6.711856638577547E-4 62-63 0.0 0.0 0.0 1.3456793142016652 7.191274969904515E-4 64-65 0.0 0.0 0.0 1.4818820621316569 7.191274969904515E-4 66-67 0.0 0.0 0.0 1.6422954357936603 7.191274969904515E-4 68-69 0.0 0.0 0.0 1.8210945024620528 7.191274969904515E-4 70-71 0.0 0.0 0.0 1.9976403029732086 7.191274969904515E-4 72-73 0.0 0.0 0.0 2.1749531728144875 7.670693301231482E-4 74-75 0.0 0.0 0.0 2.35329679206812 8.15011163255845E-4 76-77 0.0 0.0 0.0 2.5451360373486054 8.15011163255845E-4 78-79 0.0 0.0 0.0 2.764589778513525 8.15011163255845E-4 80-81 0.0 0.0 0.0 3.0025730381842317 8.15011163255845E-4 82-83 0.0 0.0 0.0 3.241898669182654 8.15011163255845E-4 84-85 0.0 0.0 0.0 3.481440038430174 8.389820798221934E-4 86-87 0.0 0.0 0.0 3.728148711731031 8.629529963885417E-4 88-89 0.0 0.0 0.0 3.991684968461465 8.629529963885417E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4485 0.0 53.640736 1 GTATCAA 7820 0.0 39.249203 1 TATCAAC 9980 0.0 30.482317 2 ATCAACG 10005 0.0 30.311861 3 TCAACGC 10140 0.0 29.908297 4 CAACGCA 10325 0.0 29.41845 5 AACGCAG 10735 0.0 28.424986 6 ACGCAGA 11975 0.0 25.270412 7 CGCAGAG 12110 0.0 24.831789 8 GTACATG 7815 0.0 23.515726 1 TACATGG 7815 0.0 23.112835 2 TGGTATC 2140 0.0 23.100327 2 AGGTAAG 1015 0.0 22.94714 2 GTGGTAT 2270 0.0 22.920668 1 TAAGGTA 565 0.0 22.715603 4 GCAGAGT 13075 0.0 22.59887 9 GAGTACT 8080 0.0 21.898605 12-13 ACATGGG 7895 0.0 21.37397 3 GTACTTT 8395 0.0 21.080204 14-15 AGAGTAC 11730 0.0 21.0775 10-11 >>END_MODULE