FastQCFastQC Report
Thu 26 May 2016
SRR938122_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938122_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2085861
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT100460.4816236556510717No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86590.41512833309602126No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT55510.26612511571959974No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25040.12004635016427269No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22980.11017033253893715No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22760.10911561221001784No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22160.10623910222205603No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22080.10585556755699445No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA49150.047.6492081
GTATCAA84050.039.2807161
TATCAAC107600.030.5068992
ATCAACG108400.030.2350353
TCAACGC110250.029.8109974
CAACGCA112200.029.2928925
AACGCAG114900.028.6045486
TAAGGTG13700.025.3076215
ACGCAGA130100.025.2260787
AGGTAAG12450.025.1829662
CGCAGAG130800.024.9821438
TAAGGTA6550.023.2037244
TGGTATC25700.022.9203282
GTAAGGT13800.022.370894
GTGGTAT28250.022.1966671
GCAGAGT145100.022.1600279
GTACATG78200.022.0511971
TGTAAGG9800.021.8131662
GAGTACT87900.021.5592212-13
GTGTAAG8600.021.5426161
Produced by FastQC (version 0.11.5)