##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938122_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2085861 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.473024808460394 33.0 31.0 34.0 31.0 34.0 2 32.707918216985696 34.0 31.0 34.0 31.0 34.0 3 32.66966542832912 34.0 31.0 34.0 31.0 34.0 4 36.20263143133699 37.0 35.0 37.0 35.0 37.0 5 36.16509968785073 37.0 35.0 37.0 35.0 37.0 6 36.22382747460161 37.0 37.0 37.0 35.0 37.0 7 36.18460002847745 37.0 37.0 37.0 35.0 37.0 8 36.176114324012964 37.0 37.0 37.0 35.0 37.0 9 37.878905641363446 39.0 38.0 39.0 35.0 39.0 10-11 37.844194315920376 39.0 38.0 39.0 35.0 39.0 12-13 37.78303467968384 39.0 38.0 39.0 35.0 39.0 14-15 39.04616127345015 40.0 38.0 41.0 36.0 41.0 16-17 38.97860667609203 40.0 38.0 41.0 36.0 41.0 18-19 38.9726561357636 40.0 38.0 41.0 35.5 41.0 20-21 38.973791398372185 40.0 38.5 41.0 35.0 41.0 22-23 39.00155475364849 40.0 38.5 41.0 35.0 41.0 24-25 38.94792486172377 40.0 38.0 41.0 35.0 41.0 26-27 38.77265958757559 40.0 38.0 41.0 35.0 41.0 28-29 38.69210100768939 40.0 38.0 41.0 34.5 41.0 30-31 38.473640621306984 40.0 38.0 41.0 34.0 41.0 32-33 38.264525057038796 40.0 38.0 41.0 34.0 41.0 34-35 38.05352154338185 40.0 37.5 41.0 33.5 41.0 36-37 37.974123874984954 40.0 37.5 41.0 33.0 41.0 38-39 37.927039960956165 40.0 37.0 41.0 33.0 41.0 40-41 37.80324024467593 40.0 37.0 41.0 33.0 41.0 42-43 37.60485406266285 40.0 37.0 41.0 33.0 41.0 44-45 37.348572843540396 40.0 37.0 41.0 32.5 41.0 46-47 36.982211182816116 40.0 36.0 41.0 31.0 41.0 48-49 36.59364646062225 39.5 36.0 41.0 30.5 41.0 50-51 36.27209938725544 39.0 35.0 41.0 30.0 41.0 52-53 35.78681105787969 39.0 35.0 40.0 28.5 41.0 54-55 35.452842734966524 38.0 35.0 40.0 28.0 41.0 56-57 35.15996032333889 38.0 34.5 40.0 27.0 41.0 58-59 34.756692799759904 38.0 34.0 40.0 26.5 41.0 60-61 34.31972744109028 37.0 34.0 40.0 26.0 41.0 62-63 33.86591028836533 36.5 33.0 39.5 25.0 41.0 64-65 33.40700626743585 36.0 33.0 39.0 23.5 40.5 66-67 32.87238699031239 35.5 32.0 39.0 22.5 40.0 68-69 32.33000545098643 35.0 31.5 38.0 21.0 40.0 70-71 31.730439851936445 35.0 31.0 37.5 19.0 39.5 72-73 31.251086721502535 35.0 30.5 37.0 18.0 39.0 74-75 30.69889891991844 34.0 30.0 36.0 14.5 39.0 76-77 29.521664195265167 33.0 28.5 35.0 13.0 37.0 78-79 30.218588391076874 34.0 30.0 35.0 12.0 37.0 80-81 30.040882398203905 34.0 30.0 35.0 7.5 37.0 82-83 29.59074741797272 34.0 29.5 35.0 4.5 36.5 84-85 29.085355160291122 34.0 29.0 35.0 2.0 36.0 86-87 28.549959944598417 34.0 29.0 35.0 2.0 36.0 88-89 27.914639326398067 33.0 27.0 35.0 2.0 35.0 90-91 27.38308952514094 33.0 26.0 35.0 2.0 35.0 92-93 26.73533471309929 32.5 25.0 35.0 2.0 35.0 94-95 25.981287823109977 32.0 24.0 34.0 2.0 35.0 96-97 25.14430803394857 32.0 19.5 34.0 2.0 35.0 98-99 24.115641694245205 31.5 8.5 34.0 2.0 35.0 100-101 20.6648583007209 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.11119765678920857 1101 2 0.06326408056544608 1101 3 0.1741415638195818 1101 4 0.05968566872748937 1101 5 -7.450315437935728E-4 1101 6 0.10297679340897048 1101 7 0.0993242353255468 1101 8 0.141255453664634 1101 9 0.10085879872752912 1101 10-11 0.11886530890373592 1101 12-13 0.11501606679951948 1101 14-15 0.24500477615912075 1101 16-17 0.27959668454442976 1101 18-19 0.2977014160150375 1101 20-21 0.19950641681565884 1101 22-23 0.17685606166006806 1101 24-25 0.13500249691616517 1101 26-27 0.24437734687032986 1101 28-29 0.20265421547286167 1101 30-31 0.2740248110116781 1101 32-33 0.262059408901969 1101 34-35 0.11985684607901703 1101 36-37 0.262265568838842 1101 38-39 0.4453046676968029 1101 40-41 0.4581471061234552 1101 42-43 0.45605370928855393 1101 44-45 0.5428451921018365 1101 46-47 0.5795643647948836 1101 48-49 0.6718242147344782 1101 50-51 0.486459570635553 1101 52-53 0.503882543118884 1101 54-55 0.5546073969505727 1101 56-57 0.7151617327363624 1101 58-59 0.6613153736869819 1101 60-61 0.7048400296984809 1101 62-63 0.6960545675072183 1101 64-65 0.7189660905423452 1101 66-67 0.7990888825135087 1101 68-69 0.8052117582682925 1101 70-71 1.0472714024844691 1101 72-73 0.9701117923405036 1101 74-75 1.0788559601360568 1101 76-77 1.0774783929543332 1101 78-79 1.0899365775706684 1101 80-81 1.2160371676511268 1101 82-83 1.2686773296883764 1101 84-85 1.4585974482800381 1101 86-87 1.6250959846224582 1101 88-89 1.63759817601942 1101 90-91 1.7944095775066593 1101 92-93 1.9555051680621496 1101 94-95 2.235556976831962 1101 96-97 2.418310743713281 1101 98-99 2.7316261029425526 1101 100-101 3.190230892034581 1102 1 0.06509219458222049 1102 2 0.09982889930981287 1102 3 0.23694373539768776 1102 4 0.08193705940699658 1102 5 0.014567544367722007 1102 6 0.041799598141935235 1102 7 0.12318012160002212 1102 8 0.0346574743076431 1102 9 0.15894418361074258 1102 10-11 0.15090544488532487 1102 12-13 0.1280023963103929 1102 14-15 0.24338679958646026 1102 16-17 0.2732982162168227 1102 18-19 0.2722971616777272 1102 20-21 0.27630845410249094 1102 22-23 0.30901267847390557 1102 24-25 0.15925684978127919 1102 26-27 0.24826803480721793 1102 28-29 0.19239376557634102 1102 30-31 0.25824260450229275 1102 32-33 0.22344833329363922 1102 34-35 0.1405712756768196 1102 36-37 0.3224463383797129 1102 38-39 0.42074867795582094 1102 40-41 0.1958536859034936 1102 42-43 0.34282995902054836 1102 44-45 0.4030916554840047 1102 46-47 0.36310730572720473 1102 48-49 0.3654805332816764 1102 50-51 0.5517965548941746 1102 52-53 0.4612358590634855 1102 54-55 0.4256561418326683 1102 56-57 0.5707665917992202 1102 58-59 0.5469075010034103 1102 60-61 0.7554823593980871 1102 62-63 0.5708681346594773 1102 64-65 0.6765920074108678 1102 66-67 0.814167485687193 1102 68-69 0.8130313734537822 1102 70-71 0.9081504348198948 1102 72-73 0.8731248427046552 1102 74-75 1.0072862273160688 1102 76-77 1.0438696674433707 1102 78-79 1.1283126434428752 1102 80-81 1.2173163050344762 1102 82-83 1.2988399802767283 1102 84-85 1.4574688052726614 1102 86-87 1.3979465658729602 1102 88-89 1.6183571913584416 1102 90-91 1.6202647705477808 1102 92-93 1.6822773786858356 1102 94-95 1.8528195761666737 1102 96-97 2.046630985375362 1102 98-99 2.1060685543339055 1102 100-101 2.404997125400154 1103 1 0.249548426953055 1103 2 0.2686775252090854 1103 3 0.33752154401918943 1103 4 0.2282922385327808 1103 5 0.07855464112542165 1103 6 0.08957808311009074 1103 7 0.1677136251702649 1103 8 0.21599077952377144 1103 9 0.2864183381454737 1103 10-11 0.27709339691736545 1103 12-13 0.2462772714124739 1103 14-15 0.4505051819779311 1103 16-17 0.34660472956802124 1103 18-19 0.4217738301464493 1103 20-21 0.3784290347483861 1103 22-23 0.4056609475402482 1103 24-25 0.466643431994072 1103 26-27 0.40760793223053327 1103 28-29 0.3527904546473124 1103 30-31 0.4049203327341431 1103 32-33 0.4253056026782218 1103 34-35 0.37130731388555205 1103 36-37 0.5136101223935512 1103 38-39 0.5919479663348568 1103 40-41 0.5593799480146586 1103 42-43 0.5747579431828953 1103 44-45 0.6027313398555663 1103 46-47 0.5793324683277419 1103 48-49 0.6641983886033174 1103 50-51 0.6406138620580535 1103 52-53 0.5659873157265665 1103 54-55 0.6550631626994701 1103 56-57 0.6796102205038181 1103 58-59 0.8004889303544545 1103 60-61 0.9818726914239662 1103 62-63 0.9172313974825173 1103 64-65 0.9219256692043061 1103 66-67 0.6660115272035227 1103 68-69 0.6561424658200892 1103 70-71 0.6112525296796889 1103 72-73 0.7297700572197847 1103 74-75 0.7456062433173152 1103 76-77 0.5660028624911639 1103 78-79 1.0345144368095163 1103 80-81 1.1156416057927423 1103 82-83 1.16239739497615 1103 84-85 1.2291046772064504 1103 86-87 1.385461087771791 1103 88-89 1.3619702717391462 1103 90-91 1.5017774188181257 1103 92-93 1.6893515723290484 1103 94-95 1.640493918831087 1103 96-97 1.80197341462544 1103 98-99 2.2846222498935553 1103 100-101 2.478356678029815 1104 1 0.12363047903649971 1104 2 0.10549826150897701 1104 3 0.15483289040594173 1104 4 0.105092174129922 1104 5 0.050885089352746604 1104 6 0.03977981559435051 1104 7 0.08064930636664513 1104 8 0.07180148010611731 1104 9 0.01647712943167079 1104 10-11 0.08401993030326338 1104 12-13 0.062037888117210116 1104 14-15 0.2274428023633277 1104 16-17 0.22351846525348407 1104 18-19 0.19729686659917434 1104 20-21 0.24545829306186562 1104 22-23 0.131755381783627 1104 24-25 0.13473341367950553 1104 26-27 0.1755341838539195 1104 28-29 0.22840695811665768 1104 30-31 0.17162645207121585 1104 32-33 0.232493032755535 1104 34-35 0.34587247620088135 1104 36-37 0.3001199728344872 1104 38-39 0.31651742020098084 1104 40-41 0.2635013462196625 1104 42-43 0.23418047210795123 1104 44-45 0.336381863187988 1104 46-47 0.37375771424839854 1104 48-49 0.36950913831652343 1104 50-51 0.5345721576553402 1104 52-53 0.4617255690121098 1104 54-55 0.4848555226735698 1104 56-57 0.5897350839141211 1104 58-59 0.4819795013734094 1104 60-61 0.5911224040380461 1104 62-63 0.5188060414613815 1104 64-65 0.6660088045343642 1104 66-67 0.6369592081678661 1104 68-69 0.7729223702339283 1104 70-71 0.6395743971731704 1104 72-73 0.821923415343484 1104 74-75 0.8652074724174632 1104 76-77 0.8857768191115767 1104 78-79 0.8856915943940749 1104 80-81 1.016363237458112 1104 82-83 1.03286063384801 1104 84-85 1.1295146534934126 1104 86-87 1.3608070163627524 1104 88-89 1.5345227263286212 1104 90-91 1.4632566068685513 1104 92-93 1.7301827184513208 1104 94-95 1.776747844860143 1104 96-97 2.0392838908632704 1104 98-99 2.2625466605896385 1104 100-101 2.3435541840764635 1105 1 0.09073297997547769 1105 2 0.09375267288535127 1105 3 0.1909412669599817 1105 4 0.0793346355220379 1105 5 0.06678889234833463 1105 6 0.017950058063554764 1105 7 -0.012457461710923212 1105 8 -0.009248766286091836 1105 9 0.04633863728560783 1105 10-11 0.049010126039206625 1105 12-13 0.06732808977671567 1105 14-15 0.10607497462676463 1105 16-17 0.13159272205728456 1105 18-19 0.10207486408177147 1105 20-21 0.0815423244033937 1105 22-23 0.09345257290591746 1105 24-25 0.20243588740913765 1105 26-27 0.1586918316897865 1105 28-29 0.08034184921088894 1105 30-31 0.11794384811422987 1105 32-33 0.0834058371873283 1105 34-35 0.14983623264546964 1105 36-37 0.15201884516510944 1105 38-39 0.21673340259431484 1105 40-41 0.24478417401373775 1105 42-43 0.16855352538598822 1105 44-45 0.24632887871879916 1105 46-47 0.38550384532327797 1105 48-49 0.3839962756037707 1105 50-51 0.46767045047443645 1105 52-53 0.47910310358849983 1105 54-55 0.41453821689444226 1105 56-57 0.3309451494660962 1105 58-59 0.3052677584536454 1105 60-61 0.42402409082278325 1105 62-63 0.4584205842758706 1105 64-65 0.4122803742950154 1105 66-67 0.4932000773570451 1105 68-69 0.7226672988028682 1105 70-71 0.682350693094655 1105 72-73 0.7554582729359254 1105 74-75 0.6427919326209626 1105 76-77 0.5576046563649157 1105 78-79 0.7707168802170656 1105 80-81 1.049618428879402 1105 82-83 1.015248977308186 1105 84-85 1.1643688827363512 1105 86-87 1.2065844347243804 1105 88-89 1.3583629818101457 1105 90-91 1.2296088543200625 1105 92-93 1.5423169842511335 1105 94-95 1.4738121420418473 1105 96-97 1.6285726699986007 1105 98-99 1.9866294785761944 1105 100-101 2.290607361007357 1106 1 0.0691975149024131 1106 2 0.094350016590532 1106 3 0.14203979374642728 1106 4 0.07648016314264083 1106 5 0.08940234452857965 1106 6 0.08010638578611662 1106 7 -0.011668243967115188 1106 8 -0.05072869649590217 1106 9 -0.06882390515401227 1106 10-11 0.10976051324783498 1106 12-13 0.060497714732186125 1106 14-15 0.07979398116644632 1106 16-17 0.05604130780080396 1106 18-19 -0.003305296738915331 1106 20-21 0.04530528308829673 1106 22-23 0.08844361700947445 1106 24-25 0.1596563885858302 1106 26-27 0.1898547523406151 1106 28-29 0.08960685238714206 1106 30-31 0.07276475254583659 1106 32-33 0.10768610190961425 1106 34-35 0.05564812070092273 1106 36-37 0.1171757123071373 1106 38-39 0.03812774122409479 1106 40-41 0.043675265585356726 1106 42-43 0.09915656142530338 1106 44-45 0.20169005444454058 1106 46-47 -0.08928622722895341 1106 48-49 0.0015717245858226647 1106 50-51 0.18512229935436153 1106 52-53 0.055476424451079254 1106 54-55 -0.0024202537693867043 1106 56-57 0.1986407592986552 1106 58-59 0.24995926822973757 1106 60-61 0.3226869140458888 1106 62-63 0.17698587756310502 1106 64-65 0.33827992410441965 1106 66-67 0.3420392181276384 1106 68-69 0.06649205327969554 1106 70-71 0.27753576210010067 1106 72-73 0.3252630490874928 1106 74-75 0.3988348468149354 1106 76-77 0.3851800648851871 1106 78-79 0.5275398550142754 1106 80-81 0.6433595445896678 1106 82-83 0.6247040656863057 1106 84-85 0.7543416221418049 1106 86-87 0.8121305953376456 1106 88-89 0.7743620822808026 1106 90-91 0.7736719500000397 1106 92-93 0.657865084900461 1106 94-95 1.2485478680295365 1106 96-97 1.2409163038373414 1106 98-99 1.4848703492797135 1106 100-101 1.8581530918738842 1107 1 0.08010376796301699 1107 2 0.030557381168769382 1107 3 0.18008350945085994 1107 4 0.028368579452077824 1107 5 0.02399774867097193 1107 6 -0.02971439775710394 1107 7 -0.0577800442422145 1107 8 -0.061922348539802385 1107 9 0.08892790594617139 1107 10-11 0.02894777045729313 1107 12-13 0.011264074060008511 1107 14-15 0.08295443821366177 1107 16-17 0.16805526859983644 1107 18-19 0.09191207773057641 1107 20-21 0.06338967775833027 1107 22-23 0.09242237433188194 1107 24-25 0.07894489803076254 1107 26-27 0.0925787894763701 1107 28-29 0.08637030086848796 1107 30-31 0.07424088176190935 1107 32-33 -0.006078238792362356 1107 34-35 0.052497084873991184 1107 36-37 0.186715395625761 1107 38-39 0.19682830026481213 1107 40-41 0.11403604448629778 1107 42-43 -0.005637328157582999 1107 44-45 0.0023076309629672664 1107 46-47 0.15865202671455592 1107 48-49 0.14802407609135315 1107 50-51 0.3137466997350984 1107 52-53 0.14824759460598358 1107 54-55 0.19608185503101083 1107 56-57 0.1891987295164128 1107 58-59 0.2493192464304883 1107 60-61 0.28611042398672737 1107 62-63 0.2824355254889994 1107 64-65 0.3076215133255502 1107 66-67 0.34041638705301125 1107 68-69 0.4604953533985636 1107 70-71 0.6270697636981879 1107 72-73 0.5715754695894759 1107 74-75 0.5766766336746301 1107 76-77 0.5136571422280234 1107 78-79 0.628756993849727 1107 80-81 0.8621379829571829 1107 82-83 0.8328044308776477 1107 84-85 1.01911621789381 1107 86-87 1.1407043488946762 1107 88-89 1.2297514654559087 1107 90-91 1.3082883085928039 1107 92-93 1.4430380719635707 1107 94-95 1.7072755726082285 1107 96-97 1.732142041865714 1107 98-99 1.8196409881192004 1107 100-101 2.074882466596815 1108 1 0.03768567441571946 1108 2 0.060812260907482596 1108 3 0.1061016164028672 1108 4 0.05485073657693107 1108 5 0.0038824406593604976 1108 6 0.053552026305069944 1108 7 0.002513162407389302 1108 8 -0.02899064067877788 1108 9 0.006482814826426875 1108 10-11 0.03804181337316237 1108 12-13 -0.0013691867689615833 1108 14-15 0.1493436559601662 1108 16-17 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-3.327929610730518 2316 100-101 -3.846366731975113 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 8.0 4 8.0 5 40.0 6 82.0 7 196.0 8 473.0 9 870.0 10 1495.0 11 2487.0 12 3924.0 13 5635.0 14 7917.0 15 10890.0 16 14407.0 17 19996.0 18 20112.0 19 15298.0 20 13944.0 21 14032.0 22 15016.0 23 16020.0 24 18446.0 25 21256.0 26 24873.0 27 30212.0 28 36524.0 29 44265.0 30 53399.0 31 65749.0 32 82080.0 33 107770.0 34 148985.0 35 221015.0 36 335212.0 37 447523.0 38 268037.0 39 17660.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.4931186689813 18.48459700814196 14.91676578640667 23.105518536470072 2 15.972324444582961 19.58846276482251 39.570722024170564 24.86849076642397 3 20.000191778917266 23.216132442520262 29.38844100520919 27.395234773353277 4 13.404824194900812 16.380717602946696 35.654724835451646 34.559733366700854 5 14.099391329027508 36.89147285335133 33.76153483270178 15.247600984919382 6 33.33163937962941 35.39890212623151 16.41613730613419 14.85332118800489 7 29.429525745004103 32.470955638942385 19.517216151987117 18.582302464066398 8 27.149176573465965 34.59647521378503 18.701425840793494 19.552922371955507 9 26.335551601952385 16.646315358501838 19.6463714504466 37.37176158909918 10-11 25.704085746845067 26.183096572590408 26.1084511384028 22.004366542161726 12-13 26.535756697114525 23.39194701852137 26.888584618054605 23.1837116663095 14-15 23.117240314671015 24.71113846991722 25.439686537118245 26.731934678293516 16-17 22.60875068107383 27.985021050065427 26.345505847586644 23.060722421274097 18-19 22.64700284438896 26.242951951256575 28.811915079672133 22.298130124682324 20-21 23.011528572613418 25.32970797191184 29.382255097535264 22.276508357939477 22-23 22.979862991829272 25.26865404741735 29.21242115366268 22.539061807090693 24-25 22.83103468527479 25.413846542918815 29.20605850675571 22.54906026505068 26-27 22.238938912418796 25.83281684754658 29.118265854515247 22.80997838551938 28-29 22.495765529468223 26.141868785827597 29.00007047462985 22.362295210074322 30-31 22.448443914846614 25.713932314233052 28.870089284537144 22.96753448638319 32-33 22.21284925682692 25.70309226353998 29.01011896714236 23.073939512490735 34-35 22.969256241462 25.871397616623888 28.716622976712387 22.44272316520173 36-37 23.05938421079759 25.44386230505622 28.67786435952481 22.818889124621382 38-39 22.550743290271736 25.781022415830645 28.65463537741854 23.013598916479076 40-41 23.016534265785907 25.636275883267285 28.65730809464771 22.6898817562991 42-43 22.685366109784745 25.661178404406463 28.548210221047327 23.10524526476146 44-45 22.662630228123664 25.93203996611156 28.619285820794975 22.786043984969794 46-47 23.089605748514018 26.2253179316012 27.960913326423203 22.724162993461583 48-49 22.87008307380759 26.155952280502586 27.921822024147403 23.052142621542426 50-51 22.99577968042933 26.531441932132584 27.805136583885503 22.667641803552584 52-53 23.42881428820041 26.19170213163773 27.64033653249186 22.739147047670002 54-55 22.915477109932063 26.445865759990717 27.793366863851425 22.845290266225792 56-57 22.893519750357285 26.56118983959142 27.68628877954955 22.859001630501744 58-59 22.95330321627376 26.176792221533457 27.583669285729012 23.28623527646377 60-61 22.608577465133102 26.528996898642816 27.821388865317488 23.041036770906594 62-63 22.67898004708847 26.56730242331584 27.949417530698355 22.80429999889734 64-65 22.44941057913255 26.592615711209906 27.777210466085705 23.18076324357184 66-67 22.388160236641852 26.549983759637847 27.73594968963529 23.325906314085007 68-69 22.775200499171444 26.238693793173212 27.940368860465714 23.045736847189634 70-71 22.703694062068376 26.391715459467335 27.41196561036426 23.49262486810003 72-73 22.731500325285335 26.549420119557343 27.35740780425925 23.36167175089807 74-75 22.567299546805852 26.9492789788006 27.29345339886023 23.18996807553332 76-77 22.754416521522767 26.473096721210087 27.488576659710308 23.283910097556838 78-79 22.821583029741674 26.661675921837553 27.496654858593168 23.02008618982761 80-81 22.959385959234943 26.67534416342459 27.25791102449595 23.107358852844516 82-83 22.825503569676105 26.782650676576054 27.34794028382419 23.043905469923654 84-85 22.775648008223317 26.80762459631331 27.279319543421725 23.13740785204164 86-87 22.989168397782954 26.880955930965015 26.99364429024072 23.136231381011303 88-89 22.863519124006405 27.06164363859511 26.76150364837525 23.313333589023234 90-91 22.770353313105932 26.953383726842343 26.95352755761681 23.322735402434912 92-93 22.8921782486756 26.610110985928998 27.10475345782295 23.392957307572452 94-95 22.64870252495495 26.442319447287666 27.273487805263922 23.635490222493466 96-97 22.87624361413252 26.24903157264931 27.235580979703293 23.639143833514872 98-99 22.650218123616728 26.5005032699965 26.898516845701664 23.950761760685108 100-101 22.918470018081656 26.07107387973673 27.062652473337167 23.94780362884445 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1050.0 1 709.5 2 530.0 3 687.5 4 546.0 5 497.5 6 1043.5 7 2469.5 8 4028.5 9 4517.5 10 3557.5 11 2476.5 12 2267.5 13 2333.5 14 2501.5 15 2641.5 16 2766.0 17 2955.0 18 3094.0 19 3352.0 20 3762.0 21 4298.5 22 5119.0 23 6148.5 24 7496.5 25 9238.5 26 11428.0 27 14193.0 28 16598.5 29 18813.5 30 21175.0 31 24804.5 32 30741.5 33 33335.5 34 33325.0 35 35076.0 36 38338.0 37 42002.0 38 44926.0 39 47713.5 40 51120.5 41 55249.5 42 57989.0 43 60871.0 44 64204.0 45 67772.5 46 70652.0 47 74590.0 48 77387.0 49 76731.5 50 76979.5 51 76978.5 52 76848.5 53 78711.5 54 81304.5 55 78033.0 56 72295.5 57 67742.5 58 63601.5 59 57407.5 60 46960.5 61 38494.0 62 31947.0 63 24181.5 64 17461.5 65 13067.0 66 9593.5 67 7091.5 68 5364.0 69 4075.0 70 2985.0 71 1965.0 72 1315.5 73 990.0 74 692.0 75 454.5 76 315.5 77 181.5 78 96.5 79 54.0 80 32.5 81 16.0 82 10.5 83 7.5 84 4.5 85 1.5 86 0.5 87 0.0 88 0.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01275252761329734 3 0.0060406709747197924 4 0.0 5 2.397091656634838E-4 6 5.273601644596644E-4 7 0.0 8 1.9176733253078705E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.3970916566348382E-5 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0016060514099453416 26-27 0.02174162132567798 28-29 1.9176733253078705E-4 30-31 7.191274969904514E-5 32-33 0.003595637484952257 34-35 0.015916688600055327 36-37 0.00783848971719592 38-39 0.02097455199555483 40-41 0.022268981490137646 42-43 0.01821789659042477 44-45 0.009300715627743173 46-47 0.0016779641596443868 48-49 2.397091656634838E-4 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 4.0750558162792243E-4 68-69 2.1573824909713543E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 2.8765099879618055E-4 82-83 0.001318400411149161 84-85 0.004075055816279225 86-87 9.588366626539352E-4 88-89 4.7941833132696764E-5 90-91 0.003667550234651302 92-93 5.273601644596644E-4 94-95 9.588366626539353E-5 96-97 2.397091656634838E-4 98-99 1.198545828317419E-4 100-101 0.0021813534075377024 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2085861.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.88413945463667 #Duplication Level Percentage of deduplicated Percentage of total 1 74.78280502521476 39.548242897623616 2 14.202137729195542 15.021356644494679 3 4.66099483646217 7.394781027864207 4 2.095076310613419 4.43185231114343 5 1.1337302796324116 2.9978175106012346 6 0.6953814028251133 2.20647882486985 7 0.46423427836714165 1.7185441221753375 8 0.3318615750463026 1.404017105150723 9 0.2321127060319269 1.104757264348696 >10 1.2660213709868569 12.178982307285825 >50 0.0855435772113388 3.11548544600537 >100 0.04346571704164133 4.326894890709386 >500 0.004090186461799034 1.5267838221768277 >1k 0.002272325812110568 1.8580684647623067 >5k 1.8178606496884543E-4 0.6830462523418026 >10k+ 9.089303248442272E-5 0.48289110844656924 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10046 0.4816236556510717 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8659 0.41512833309602126 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5551 0.26612511571959974 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2504 0.12004635016427269 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2298 0.11017033253893715 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2276 0.10911561221001784 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2216 0.10623910222205603 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2208 0.10585556755699445 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 4.7941833132696764E-5 0.0 0.0 0.0 0.0 8 4.7941833132696764E-5 0.0 0.0 0.0 0.0 9 4.7941833132696764E-5 0.0 0.0 0.0 0.0 10-11 4.7941833132696764E-5 0.0 0.0 0.0 0.0 12-13 4.7941833132696764E-5 0.0 0.0 4.7941833132696764E-5 1.198545828317419E-4 14-15 4.7941833132696764E-5 0.0 0.0 4.7941833132696764E-5 1.6779641596443868E-4 16-17 4.7941833132696764E-5 0.0 0.0 4.7941833132696764E-5 1.9176733253078705E-4 18-19 4.7941833132696764E-5 0.0 0.0 9.588366626539353E-5 1.9176733253078705E-4 20-21 4.7941833132696764E-5 0.0 0.0 9.588366626539353E-5 1.9176733253078705E-4 22-23 4.7941833132696764E-5 0.0 0.0 1.9176733253078705E-4 1.9176733253078705E-4 24-25 4.7941833132696764E-5 0.0 0.0 4.5544741476061923E-4 1.9176733253078705E-4 26-27 4.7941833132696764E-5 0.0 0.0 0.0011745749117510708 1.9176733253078705E-4 28-29 4.7941833132696764E-5 0.0 0.0 0.0031401900701916374 1.9176733253078705E-4 30-31 4.7941833132696764E-5 0.0 0.0 0.010691028788591378 1.9176733253078705E-4 32-33 4.7941833132696764E-5 0.0 0.0 0.027039193886840972 2.397091656634838E-4 34-35 4.7941833132696764E-5 0.0 0.0 0.05230453994777216 2.8765099879618055E-4 36-37 4.7941833132696764E-5 0.0 0.0 0.09125727936808828 2.8765099879618055E-4 38-39 4.7941833132696764E-5 0.0 0.0 0.1504175014538361 2.8765099879618055E-4 40-41 4.7941833132696764E-5 0.0 0.0 0.2117590769471216 2.8765099879618055E-4 42-43 4.7941833132696764E-5 0.0 0.0 0.2750422966822813 2.8765099879618055E-4 44-45 4.7941833132696764E-5 0.0 0.0 0.35313954285544435 2.8765099879618055E-4 46-47 4.7941833132696764E-5 0.0 0.0 0.4427188580638882 2.8765099879618055E-4 48-49 4.7941833132696764E-5 0.0 0.0 0.5362054326726469 2.8765099879618055E-4 50-51 4.7941833132696764E-5 0.0 0.0 0.6366196021690802 2.8765099879618055E-4 52-53 4.7941833132696764E-5 0.0 0.0 0.7307533915251304 2.8765099879618055E-4 54-55 4.7941833132696764E-5 0.0 0.0 0.8348351112562151 3.1162191536252893E-4 56-57 4.7941833132696764E-5 0.0 0.0 0.9533952645933741 3.3559283192887736E-4 58-59 4.7941833132696764E-5 0.0 0.0 1.0821670283877975 3.3559283192887736E-4 60-61 4.7941833132696764E-5 0.0 0.0 1.2182738926515237 3.3559283192887736E-4 62-63 4.7941833132696764E-5 0.0 0.0 1.3514083632610228 3.3559283192887736E-4 64-65 4.7941833132696764E-5 0.0 0.0 1.4880186167726421 3.3559283192887736E-4 66-67 4.7941833132696764E-5 0.0 0.0 1.649366856180733 3.3559283192887736E-4 68-69 4.7941833132696764E-5 0.0 0.0 1.8283576901816563 3.3559283192887736E-4 70-71 4.7941833132696764E-5 0.0 0.0 2.0051192289419095 3.5956374849522573E-4 72-73 4.7941833132696764E-5 0.0 0.0 2.1837025573612046 4.3147649819427086E-4 74-75 4.7941833132696764E-5 0.0 0.0 2.364419297354905 4.794183313269676E-4 76-77 4.7941833132696764E-5 0.0 0.0 2.5593028490393177 4.794183313269676E-4 78-79 4.7941833132696764E-5 0.0 0.0 2.7829994424364806 4.794183313269676E-4 80-81 4.7941833132696764E-5 0.0 0.0 3.02405097942768 4.794183313269676E-4 82-83 4.7941833132696764E-5 0.0 0.0 3.266684596912258 5.273601644596644E-4 84-85 4.7941833132696764E-5 0.0 0.0 3.511475596887808 5.273601644596644E-4 86-87 4.7941833132696764E-5 0.0 0.0 3.764153028413686 5.992729141587095E-4 88-89 4.7941833132696764E-5 2.3970916566348382E-5 0.0 4.03576748402698 6.711856638577547E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4915 0.0 47.649208 1 GTATCAA 8405 0.0 39.280716 1 TATCAAC 10760 0.0 30.506899 2 ATCAACG 10840 0.0 30.235035 3 TCAACGC 11025 0.0 29.810997 4 CAACGCA 11220 0.0 29.292892 5 AACGCAG 11490 0.0 28.604548 6 TAAGGTG 1370 0.0 25.307621 5 ACGCAGA 13010 0.0 25.226078 7 AGGTAAG 1245 0.0 25.182966 2 CGCAGAG 13080 0.0 24.982143 8 TAAGGTA 655 0.0 23.203724 4 TGGTATC 2570 0.0 22.920328 2 GTAAGGT 1380 0.0 22.37089 4 GTGGTAT 2825 0.0 22.196667 1 GCAGAGT 14510 0.0 22.160027 9 GTACATG 7820 0.0 22.051197 1 TGTAAGG 980 0.0 21.813166 2 GAGTACT 8790 0.0 21.55922 12-13 GTGTAAG 860 0.0 21.542616 1 >>END_MODULE