FastQCFastQC Report
Thu 26 May 2016
SRR938121_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938121_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107464
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106580.5057263137116458No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86940.41253373723109865No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54680.2594587618104034No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25900.12289652397383774No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA22860.10847160378540273No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22600.1072378935061287No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21740.103157159505453No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC21600.10249285397045929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA51350.048.078631
GTATCAA88300.039.3165741
TATCAAC109750.031.781422
ATCAACG110650.031.394083
TCAACGC111950.031.0719684
CAACGCA113550.030.5504445
AACGCAG117450.029.6169176
ACGCAGA132600.025.8857777
CGCAGAG132300.025.8726068
GTACATG79100.024.1482411
TACATGG77200.024.1296222
TGGTATC26900.023.3186512
GCAGAGT143800.023.241499
GTGGTAT29250.023.1827241
GTGTAGC11600.023.0532421
GAGTACT84750.022.68846112-13
ACATGGG80950.022.4247843
GTACTTT87550.021.81310814-15
AGAGTAC122900.021.08126410-11
AGTACTT87050.020.72379312-13
Produced by FastQC (version 0.11.5)