##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938121_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2107464 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.827224569435113 33.0 31.0 34.0 30.0 34.0 2 32.01346120265874 34.0 31.0 34.0 30.0 34.0 3 32.07587508019117 34.0 31.0 34.0 30.0 34.0 4 35.60065367664643 37.0 35.0 37.0 33.0 37.0 5 35.615992965953396 37.0 35.0 37.0 33.0 37.0 6 35.64207644828097 37.0 35.0 37.0 33.0 37.0 7 35.58051240732938 37.0 35.0 37.0 33.0 37.0 8 35.5632888628228 37.0 35.0 37.0 33.0 37.0 9 37.209777248864036 39.0 37.0 39.0 34.0 39.0 10-11 37.1327389222307 39.0 37.0 39.0 34.0 39.0 12-13 37.028840113045824 39.0 37.0 39.0 33.0 39.0 14-15 38.262480877490674 40.0 38.0 41.0 33.0 41.0 16-17 38.18249113626615 40.0 38.0 41.0 33.0 41.0 18-19 38.119958395493356 40.0 38.0 41.0 33.0 41.0 20-21 38.00809954523541 40.0 38.0 41.0 33.0 41.0 22-23 37.85120552474443 40.0 38.0 41.0 33.0 41.0 24-25 37.79982149161267 40.0 38.0 41.0 32.5 41.0 26-27 37.79647979751967 40.0 38.0 41.0 32.5 41.0 28-29 37.70455557010701 40.0 38.0 41.0 32.0 41.0 30-31 37.51233876355658 40.0 37.0 41.0 32.0 41.0 32-33 37.28248809944084 40.0 37.0 41.0 31.5 41.0 34-35 37.073174915443396 40.0 36.0 41.0 31.0 41.0 36-37 36.84817652875684 40.0 36.0 41.0 30.5 41.0 38-39 36.59311879111577 39.0 36.0 41.0 30.0 41.0 40-41 36.35958312929663 39.0 35.0 41.0 30.0 41.0 42-43 36.054335922226905 39.0 35.0 41.0 29.0 41.0 44-45 35.823640166569874 39.0 35.0 40.5 28.0 41.0 46-47 35.66345142787729 39.0 35.0 41.0 27.0 41.0 48-49 35.3882562169508 39.0 35.0 41.0 26.0 41.0 50-51 34.72933274305041 38.5 34.0 40.0 25.0 40.5 52-53 34.79625322188184 38.0 34.5 40.0 25.5 41.0 54-55 34.914445750911995 38.5 35.0 40.0 25.5 41.0 56-57 34.67592400154878 38.0 34.0 40.0 24.5 41.0 58-59 34.38924152441038 38.0 34.0 40.0 23.5 41.0 60-61 33.98951939392559 37.5 34.0 40.0 22.5 41.0 62-63 33.54985518139337 37.0 33.0 40.0 21.5 41.0 64-65 33.09392022829334 36.0 33.0 39.0 20.0 41.0 66-67 32.641934097094904 36.0 32.5 39.0 18.5 41.0 68-69 32.14487673336294 35.0 32.0 39.0 15.0 40.0 70-71 31.655383437154796 35.0 31.0 38.0 11.5 40.0 72-73 31.164278251016388 35.0 31.0 37.0 7.5 39.5 74-75 30.637957042208075 35.0 30.5 36.5 4.5 39.0 76-77 30.09935875535715 34.5 30.0 36.0 2.0 39.0 78-79 29.533814812495017 34.0 29.0 35.5 2.0 37.5 80-81 29.018565441687258 34.0 29.0 35.0 2.0 37.0 82-83 28.48497720483007 34.0 28.0 35.0 2.0 36.5 84-85 28.0018721553488 34.0 27.0 35.0 2.0 36.0 86-87 27.50043867890507 33.0 26.0 35.0 2.0 36.0 88-89 26.92751169177742 33.0 25.0 35.0 2.0 35.0 90-91 26.30862401445529 32.5 24.5 35.0 2.0 35.0 92-93 25.689112838938172 32.0 22.0 35.0 2.0 35.0 94-95 25.067511948009553 32.0 19.0 34.0 2.0 35.0 96-97 24.210121501482348 31.5 8.5 34.0 2.0 35.0 98-99 23.115945278306057 31.0 2.0 34.0 2.0 35.0 100-101 20.26786483659982 27.5 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4029498617951184 1101 2 0.3096815261766679 1101 3 0.2783519736092188 1101 4 0.19707983340242663 1101 5 0.10851258532370878 1101 6 0.17724833372229654 1101 7 0.23222703594819194 1101 8 0.25254558163764074 1101 9 0.20956031300568156 1101 10-11 0.29036635628270346 1101 12-13 0.1786375270664493 1101 14-15 0.19858171785647727 1101 16-17 0.323488684719365 1101 18-19 0.2710935031631436 1101 20-21 0.3167780510257643 1101 22-23 0.3542990401488737 1101 24-25 0.41988421910141227 1101 26-27 0.3084626871573448 1101 28-29 0.3619186943339656 1101 30-31 0.3067495999741041 1101 32-33 0.4808643685466194 1101 34-35 0.48459195967480184 1101 36-37 0.4733373018716165 1101 38-39 0.46469232517387127 1101 40-41 0.5380784518557888 1101 42-43 0.5918527135622327 1101 44-45 0.7029880776036563 1101 46-47 0.8194843627065609 1101 48-49 0.8835354287046826 1101 50-51 0.9029967413482751 1101 52-53 0.8607301248579446 1101 54-55 0.8116023079399142 1101 56-57 0.7932812385640418 1101 58-59 0.7997864626980586 1101 60-61 0.666311536686031 1101 62-63 0.5062583435996046 1101 64-65 0.6497530903016582 1101 66-67 0.6340882119810018 1101 68-69 0.7378786509482396 1101 70-71 0.8003185266957438 1101 72-73 0.804207661718177 1101 74-75 0.7430212179434719 1101 76-77 0.7776241025760804 1101 78-79 0.7258760706613181 1101 80-81 0.746638918342672 1101 82-83 0.6670812696425656 1101 84-85 0.6988054983449423 1101 86-87 0.885022005294509 1101 88-89 0.9940075334007865 1101 90-91 0.9811283367574823 1101 92-93 1.1274684435072473 1101 94-95 1.2922862396171304 1101 96-97 1.2310973837053112 1101 98-99 1.3849726231941162 1101 100-101 1.317121454590076 1102 1 0.2737530402217452 1102 2 0.3087207542937307 1102 3 0.29007473395670047 1102 4 0.2674453297306201 1102 5 0.14253098853582458 1102 6 0.21255327739611118 1102 7 0.1363963054050501 1102 8 0.18598571332978509 1102 9 0.2183658816074754 1102 10-11 0.37140871756496097 1102 12-13 0.3698828000636283 1102 14-15 0.37327143684813535 1102 16-17 0.27522765857676035 1102 18-19 0.25354798468991646 1102 20-21 0.4424270693540606 1102 22-23 0.3967122490442847 1102 24-25 0.2386172557554076 1102 26-27 0.3474187706595018 1102 28-29 0.39511867180659976 1102 30-31 0.2597581929276842 1102 32-33 0.27205885161311727 1102 34-35 0.22010158728592444 1102 36-37 0.3100304950392072 1102 38-39 0.2613712682266254 1102 40-41 0.33640548424096295 1102 42-43 0.5740954842640349 1102 44-45 0.40212322790413424 1102 46-47 0.4709228337798095 1102 48-49 0.34966324675789195 1102 50-51 0.34271198705941686 1102 52-53 0.4712487298347412 1102 54-55 0.4869146298457778 1102 56-57 0.3575527182354037 1102 58-59 0.25362898187862015 1102 60-61 0.3075322155763871 1102 62-63 0.34054805701109103 1102 64-65 0.37614520200024515 1102 66-67 0.5259573279313585 1102 68-69 0.6201667864100955 1102 70-71 0.5113964150953372 1102 72-73 0.6105613632087596 1102 74-75 0.48396919159057816 1102 76-77 0.485961763128838 1102 78-79 0.3923989703065125 1102 80-81 0.507519743877971 1102 82-83 0.5861204150903561 1102 84-85 0.5425895186857126 1102 86-87 0.2685990062911898 1102 88-89 0.6390441739698005 1102 90-91 0.7825523665432179 1102 92-93 0.7112868129282965 1102 94-95 0.8765130468835558 1102 96-97 0.6331215148642393 1102 98-99 1.1068295505944796 1102 100-101 1.2945960169984012 1103 1 0.3680982883132309 1103 2 0.4008618913871942 1103 3 0.3822849929056673 1103 4 0.22631831247985446 1103 5 0.17929018600187874 1103 6 0.2662901655399068 1103 7 0.3740275091524339 1103 8 0.36494974221882615 1103 9 0.5009640163882807 1103 10-11 0.4105921694033299 1103 12-13 0.33628471055276776 1103 14-15 0.4590937654466245 1103 16-17 0.39767897269518926 1103 18-19 0.41883829891690283 1103 20-21 0.3422344530285457 1103 22-23 0.45457113628950196 1103 24-25 0.5563924520103711 1103 26-27 0.509085055208871 1103 28-29 0.5461474922129241 1103 30-31 0.5137553345929291 1103 32-33 0.6573900742959822 1103 34-35 0.6504453760300706 1103 36-37 0.6530137505131037 1103 38-39 0.6507980825414705 1103 40-41 0.8169114172813536 1103 42-43 0.7093838708674411 1103 44-45 0.8349524828046029 1103 46-47 0.8711274247366276 1103 48-49 0.8279494920441053 1103 50-51 0.7884014361923022 1103 52-53 0.9029435587897936 1103 54-55 0.9632696007537476 1103 56-57 0.7694498551789408 1103 58-59 0.9873108694699368 1103 60-61 0.9335059453771066 1103 62-63 0.7693946517618215 1103 64-65 0.8886971468312623 1103 66-67 0.8401139707120464 1103 68-69 0.887734933501406 1103 70-71 0.9028147420251607 1103 72-73 0.9741540057226921 1103 74-75 0.8173856415212875 1103 76-77 0.7991409644598697 1103 78-79 0.7469068348646708 1103 80-81 0.7857608895873263 1103 82-83 0.9178717341251996 1103 84-85 0.973063884456181 1103 86-87 0.9104965618600502 1103 88-89 1.2053207159104602 1103 90-91 1.199347663141694 1103 92-93 1.3450668097448002 1103 94-95 1.2439139628941724 1103 96-97 1.136447273999881 1103 98-99 1.3286649500384904 1103 100-101 1.3563957018611497 1104 1 0.4806167235917478 1104 2 0.4428452578245512 1104 3 0.3288092086029195 1104 4 0.3200828689204869 1104 5 0.32202658259775774 1104 6 0.38220815602198144 1104 7 0.3910708982304527 1104 8 0.46299985300327506 1104 9 0.37622404538713283 1104 10-11 0.4228427316188572 1104 12-13 0.35611153296729725 1104 14-15 0.43834949852452354 1104 16-17 0.5979984023384262 1104 18-19 0.6260352796087503 1104 20-21 0.45816108234779307 1104 22-23 0.5442239517902081 1104 24-25 0.577753531906481 1104 26-27 0.6047895489246287 1104 28-29 0.631887605582115 1104 30-31 0.580935079076859 1104 32-33 0.6150044835999395 1104 34-35 0.6320163174698123 1104 36-37 0.6089456598896277 1104 38-39 0.5699271633553735 1104 40-41 0.5848964396110219 1104 42-43 0.7991614311768842 1104 44-45 0.802133540391786 1104 46-47 0.6840036460954266 1104 48-49 0.8343557081366626 1104 50-51 0.9377133700893054 1104 52-53 0.9158506726303131 1104 54-55 0.9266046480323737 1104 56-57 0.893747863270427 1104 58-59 0.8830743146105533 1104 60-61 0.8633986717526483 1104 62-63 0.8415393087447001 1104 64-65 0.8852122399164628 1104 66-67 1.058906689761855 1104 68-69 1.0913188069348365 1104 70-71 1.1003341517645175 1104 72-73 0.9916186245011751 1104 74-75 0.9337435215921097 1104 76-77 0.9386664780560174 1104 78-79 0.9180149004977203 1104 80-81 0.9947250308108906 1104 82-83 1.051719112825559 1104 84-85 1.1951673514634251 1104 86-87 1.2570075774141038 1104 88-89 1.3183205071961552 1104 90-91 1.25688439202181 1104 92-93 1.272440105647778 1104 94-95 1.3924359615202526 1104 96-97 1.284591237111357 1104 98-99 1.402981065132682 1104 100-101 1.0111404276782814 1105 1 0.35236723775405565 1105 2 0.21086062451993115 1105 3 0.2994394704994292 1105 4 0.24192289974589443 1105 5 0.23854471840385116 1105 6 0.1709191912787631 1105 7 0.16968338331653854 1105 8 0.20348292889048736 1105 9 0.28870364589551656 1105 10-11 0.34434964632210097 1105 12-13 0.16875771365602787 1105 14-15 0.2973409573917607 1105 16-17 0.2710843007322623 1105 18-19 0.2842270607094335 1105 20-21 0.3425880363525522 1105 22-23 0.36137405710002213 1105 24-25 0.4814412777897559 1105 26-27 0.2374281604010804 1105 28-29 0.34519296246980957 1105 30-31 0.2685579792397945 1105 32-33 0.278302165102879 1105 34-35 0.35153541811821043 1105 36-37 0.3639258122736635 1105 38-39 0.4753076965446681 1105 40-41 0.44395027480391747 1105 42-43 0.4238129974641254 1105 44-45 0.38185103757046335 1105 46-47 0.32250485800992124 1105 48-49 0.04410240716823921 1105 50-51 0.03900666136064501 1105 52-53 0.11238498204254199 1105 54-55 0.07888151663465237 1105 56-57 0.20307394236979803 1105 58-59 0.1819041391649705 1105 60-61 0.03373536677179345 1105 62-63 0.010304208834192252 1105 64-65 0.04609858980872161 1105 66-67 0.1868588926857555 1105 68-69 0.10686632729894541 1105 70-71 0.37861009224246445 1105 72-73 0.5253152225140703 1105 74-75 0.42608218257147357 1105 76-77 0.4083695003880692 1105 78-79 0.38081638054194045 1105 80-81 0.4581201919121334 1105 82-83 0.34919512780118467 1105 84-85 0.4551384946710648 1105 86-87 0.7349432793667496 1105 88-89 0.6366150632470315 1105 90-91 0.5114508035648804 1105 92-93 0.4842646883789179 1105 94-95 0.6764540630414793 1105 96-97 0.6827949537671181 1105 98-99 0.5767106341211168 1105 100-101 0.4306485350522671 1106 1 0.06326266726921403 1106 2 0.05722821213977625 1106 3 0.09904740720787686 1106 4 0.14951156589281567 1106 5 -0.050989854237379006 1106 6 0.14400428874805016 1106 7 0.10196336022997343 1106 8 0.07550284594388046 1106 9 0.1722094266811851 1106 10-11 -0.4722070103962466 1106 12-13 -0.21831270419462356 1106 14-15 -0.02492481796677737 1106 16-17 0.08263968360643759 1106 18-19 0.08773287552099163 1106 20-21 0.01590378680838711 1106 22-23 0.038668922465966205 1106 24-25 0.10145696372401147 1106 26-27 0.05730638174883751 1106 28-29 0.03338302342293531 1106 30-31 0.1078195791965797 1106 32-33 0.16621599964604883 1106 34-35 0.006298340618720033 1106 36-37 -0.16305278246567667 1106 38-39 0.13095486680298052 1106 40-41 0.28858413711220976 1106 42-43 0.28788297850745437 1106 44-45 0.27934443548493704 1106 46-47 0.3636958533989869 1106 48-49 0.4202695482833789 1106 50-51 0.44418469969166097 1106 52-53 0.41999474423986527 1106 54-55 0.10726314758902333 1106 56-57 0.2590734294295416 1106 58-59 0.39554928573360826 1106 60-61 0.04667254094732698 1106 62-63 0.2771332002515905 1106 64-65 0.49705941708013057 1106 66-67 0.1460183639404775 1106 68-69 -0.2010974104618377 1106 70-71 0.23547232695218767 1106 72-73 0.39832965246774066 1106 74-75 0.35148435793153965 1106 76-77 0.4762907015359339 1106 78-79 0.6332711356810172 1106 80-81 0.6066307512084101 1106 82-83 0.6434833296223346 1106 84-85 0.4682077318234228 1106 86-87 0.5323837670257738 1106 88-89 0.5393922562048665 1106 90-91 0.4874144911374785 1106 92-93 0.6038355921617864 1106 94-95 0.7039806872353331 1106 96-97 0.9362945163217979 1106 98-99 0.8539586713590488 1106 100-101 0.41180308446232416 1107 1 0.2711259636300589 1107 2 0.2566086333010915 1107 3 0.21252277614468085 1107 4 0.1287089627937732 1107 5 0.15409651384092626 1107 6 0.21324654309142232 1107 7 0.09341068313273126 1107 8 0.15882440916249863 1107 9 0.24100318847944635 1107 10-11 0.26328637438989233 1107 12-13 0.10484408560586189 1107 14-15 0.2330668852281903 1107 16-17 0.11995166370000021 1107 18-19 0.24423118656070386 1107 20-21 0.2594278696912866 1107 22-23 0.31958205410661833 1107 24-25 0.41287966559375633 1107 26-27 0.3486363719601968 1107 28-29 0.4459684283988068 1107 30-31 0.48870693940343557 1107 32-33 0.47394268079712276 1107 34-35 0.5522695429349156 1107 36-37 0.4083614162638085 1107 38-39 0.4210795972673509 1107 40-41 0.5070226256739119 1107 42-43 0.5000344858804553 1107 44-45 0.4231658212306897 1107 46-47 0.44989474909612426 1107 48-49 0.3381289881405465 1107 50-51 0.39571014749846967 1107 52-53 0.39727883223201843 1107 54-55 0.476867675828494 1107 56-57 0.49941620382775653 1107 58-59 0.5200979785958424 1107 60-61 0.368270455815221 1107 62-63 0.4715379333524723 1107 64-65 0.5940650180171545 1107 66-67 0.545744867413994 1107 68-69 0.4893056883330189 1107 70-71 0.6634766845557003 1107 72-73 0.42588860846060683 1107 74-75 0.558709509377092 1107 76-77 0.6709600063458758 1107 78-79 0.5127614344454194 1107 80-81 0.6963303860687411 1107 82-83 0.7046897684277695 1107 84-85 0.7232367436209621 1107 86-87 0.9006243948416461 1107 88-89 0.692458811188498 1107 90-91 0.8753433480607811 1107 92-93 0.7223325014320103 1107 94-95 0.6107772427470834 1107 96-97 0.766816634516676 1107 98-99 0.6848853744537102 1107 100-101 0.48209953168901976 1108 1 0.3215331298037718 1108 2 0.2597650220293488 1108 3 0.10932904059817616 1108 4 0.13378849802519 1108 5 0.1655376819725376 1108 6 0.17867385549214276 1108 7 0.11705487813504334 1108 8 0.09753699258725845 1108 9 0.1281076612464176 1108 10-11 0.19421019403522166 1108 12-13 0.07009784875263136 1108 14-15 0.1501079910537797 1108 16-17 0.3227382432935926 1108 18-19 0.4182381281220344 1108 20-21 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-1.2774787229935463 2316 74-75 -1.3669752548121004 2316 76-77 -1.4119197390340084 2316 78-79 -1.507903180406526 2316 80-81 -1.4950486601416344 2316 82-83 -1.5938488206224513 2316 84-85 -1.6980139712124007 2316 86-87 -1.6464627067213762 2316 88-89 -1.7583598382572205 2316 90-91 -1.7989703304183031 2316 92-93 -1.9330702806867812 2316 94-95 -1.9756525851532736 2316 96-97 -1.7293115853119225 2316 98-99 -1.9528351735966716 2316 100-101 -1.9905484718660453 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 6117.0 3 1815.0 4 1848.0 5 2126.0 6 2476.0 7 2982.0 8 3584.0 9 4450.0 10 5667.0 11 6708.0 12 8329.0 13 9972.0 14 11662.0 15 14313.0 16 17262.0 17 22586.0 18 20645.0 19 17067.0 20 16688.0 21 16901.0 22 18581.0 23 20412.0 24 22355.0 25 25169.0 26 28160.0 27 32870.0 28 37862.0 29 44750.0 30 52976.0 31 64973.0 32 80879.0 33 104485.0 34 143384.0 35 210320.0 36 313931.0 37 406282.0 38 276236.0 39 30638.0 40 3.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.24449059293928 18.07772180087216 14.501393594472352 23.17639401171621 2 15.820957027160853 19.168363498082254 39.997208476925074 25.01347099783182 3 19.68832212126694 23.236728668942792 29.221213618945473 27.853735590844803 4 13.167763679581695 16.040953038477284 35.91724113043453 34.87404215150649 5 14.131031508881641 36.78814375511708 33.70521424785771 15.375610488143565 6 33.33173403512439 35.11184794949385 16.58798436171375 14.968433653668015 7 29.32158062331846 32.557833646888774 19.571984953137626 18.548600776655142 8 27.1442461347976 34.217402739705214 18.932156831839603 19.706194293657582 9 26.61219684147809 16.64018344399 19.64896872099565 37.09865099353626 10-11 25.67126097220979 26.06469226051562 26.166813441626264 22.097233325648325 12-13 26.58938710363149 23.401527935956373 26.849513865549714 23.159571094862425 14-15 23.272866684154238 24.658499732345167 25.3851677679486 26.683465815551994 16-17 22.65763305127619 27.89920486996669 26.250306545145406 23.192855533611713 18-19 22.71146090984864 26.180066267409913 28.74622506752206 22.362247755219382 20-21 23.19854269784202 25.329695143291726 29.1841757728265 22.28758638603976 22-23 23.02043940458331 25.22486953685197 29.122513211315283 22.632177847249444 24-25 22.86797838525866 25.374428730365484 29.079164999412257 22.6784278849636 26-27 22.24777623140014 25.796926586315177 29.063313005669052 22.891984176615637 28-29 22.484033077959133 26.16478817964624 28.909287274966765 22.441891467427862 30-31 22.513681208433738 25.72774721680688 28.75835242849908 23.000219146260303 32-33 22.2181358437257 25.709021623642958 28.90564066645694 23.167201866174402 34-35 23.015813657968835 25.846425906258673 28.569291686878035 22.56846874889446 36-37 23.035025552449294 25.41859126171525 28.60255109005977 22.943832095775683 38-39 22.5444007901913 25.812306543525438 28.584242172793466 23.0590504934898 40-41 23.022596724424403 25.588772845953002 28.640636126275815 22.747994303346783 42-43 22.681302092419653 25.609289199193462 28.520518909629928 23.18888979875696 44-45 22.646538077011886 25.922496418463055 28.585399099614545 22.84556640491052 46-47 23.099893870297734 26.2022890980684 27.93475206658642 22.763064965047445 48-49 22.884834314542857 26.256260268644226 27.82980280643248 23.029102610380434 50-51 22.96855855173836 26.53624344381479 27.760491854204634 22.734706150242218 52-53 23.34497915665001 26.211622060204842 27.599604538786004 22.84379424435915 54-55 22.86832375374363 26.464989012345093 27.787447019568944 22.87924021434233 56-57 22.850330601537696 26.600549523308224 27.677027619028184 22.872092256125896 58-59 22.98368734259191 26.179891774188075 27.57074272294966 23.265678160270358 60-61 22.61406470301634 26.451633879435164 27.876800414269415 23.05750100327908 62-63 22.722967862974542 26.47157164048164 27.979480067744824 22.825980428799 64-65 22.47006551805395 26.531240381655895 27.845517024038948 23.153177076251204 66-67 22.403547804429394 26.524721584960602 27.752353227602217 23.319377383007783 68-69 22.799863221513046 26.14680716914805 27.98427569455304 23.069053914785865 70-71 22.69895533451583 26.329306150621427 27.51474620109235 23.456992313770392 72-73 22.73882704768234 26.443476609783307 27.416894120997792 23.400802221536562 74-75 22.527224635729212 26.871964410100258 27.441388566186987 23.159422387983543 76-77 22.803848854596268 26.45381542973315 27.459598868419473 23.282736847251105 78-79 22.839420631003453 26.62608204563575 27.4256330916132 23.108864231747596 80-81 23.0232579661613 26.613194677289876 27.149528175068443 23.21401918148038 82-83 22.821970730688637 26.721879842523794 27.291698801842447 23.164450624945125 84-85 22.88365993901905 26.76128229423913 27.196440143964207 23.158617622777612 86-87 23.02359649630658 26.83275738849749 26.88467309712793 23.258973018067998 88-89 22.9578951765729 26.90759591977791 26.729603636653643 23.404905266995545 90-91 22.888162553354523 26.79855282563856 26.876912555431616 23.436372065575295 92-93 23.03932213735965 26.460675370637897 27.008654843183034 23.49134764881942 94-95 22.876897943448512 26.318323234231894 27.166567044565976 23.638211777753618 96-97 23.110428994978204 26.036895509719482 27.137551497935096 23.71512399736722 98-99 22.89936942575237 26.261095094982483 26.79647444226967 24.04306103699548 100-101 23.275440423570952 25.662946620610917 26.996421283689198 24.065191672128936 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1131.0 1 764.5 2 574.0 3 746.0 4 585.0 5 535.5 6 1069.5 7 2432.5 8 4086.0 9 4687.0 10 3674.5 11 2566.5 12 2372.5 13 2455.0 14 2506.5 15 2627.0 16 2820.0 17 2950.0 18 3125.0 19 3380.0 20 3791.0 21 4431.5 22 5239.0 23 6288.5 24 7751.5 25 9526.5 26 11742.5 27 14600.0 28 16825.0 29 18852.0 30 21435.5 31 24696.5 32 30253.0 33 33093.0 34 33334.0 35 35437.0 36 38805.0 37 42390.0 38 45291.0 39 47936.5 40 51314.0 41 55399.5 42 58044.5 43 60892.5 44 64002.0 45 67630.0 46 70758.0 47 74534.5 48 77231.0 49 76717.0 50 76881.5 51 77076.5 52 77140.5 53 79134.5 54 81744.0 55 78896.5 56 73477.5 57 68645.0 58 65193.0 59 58870.0 60 47958.0 61 39788.0 62 33080.5 63 25145.5 64 18548.5 65 14175.0 66 10291.5 67 7636.5 68 5838.5 69 4367.5 70 3339.5 71 2293.0 72 1577.5 73 1184.5 74 842.0 75 552.0 76 383.0 77 248.0 78 140.5 79 76.5 80 42.0 81 27.0 82 17.5 83 13.5 84 12.5 85 10.5 86 12.0 87 13.0 88 9.0 89 5.0 90 3.0 91 1.5 92 1.5 93 0.5 94 0.5 95 1.5 96 1.5 97 0.5 98 0.0 99 0.5 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4920131494535612 2 0.0515785797527265 3 0.05936044459122433 4 0.06842347010435292 5 0.08408210057206197 6 0.11264723857679182 7 0.07165009698860811 8 0.052480137264503686 9 0.052147984497006826 10-11 0.07746277041980314 12-13 0.11086784875091579 14-15 0.057248901997851445 16-17 0.06313275102208152 18-19 0.061709239161380686 20-21 0.07853040431532876 22-23 0.06688133225526036 24-25 0.09283669851537203 26-27 0.06960972998827027 28-29 0.069728355976662 30-31 0.07423614353554794 32-33 0.07361928839591092 34-35 0.07565965539624876 36-37 0.0712467686280762 38-39 0.04201732508835264 40-41 0.04574218112385312 42-43 0.04420004327476057 44-45 0.022657563782821438 46-47 0.028849840376870023 48-49 0.029680222295612166 50-51 0.02749750410920424 52-53 0.02642987021367862 54-55 0.02628751902760854 56-57 0.025955366260111683 58-59 0.02279991496889152 60-61 0.029537871109542084 62-63 0.020854448759267064 64-65 0.02313206773638838 66-67 0.01698724153769649 68-69 0.019336036107852853 70-71 0.042918882600129826 72-73 0.03862462182034901 74-75 0.03257469641237051 76-77 0.02370147248066871 78-79 0.016322936002702775 80-81 0.018007425037865416 82-83 0.02272873937585648 84-85 0.019573288084636323 86-87 0.00958497986205221 88-89 0.043274760565305034 90-91 0.054686580648589965 92-93 0.03437781143592488 94-95 0.05556441296268881 96-97 0.04495924960046767 98-99 0.03480486499413513 100-101 0.17257708791229648 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2107464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 54.95919709437902 #Duplication Level Percentage of deduplicated Percentage of total 1 76.91183004960872 42.270124265858286 2 12.868140088807733 14.144452947577282 3 4.38745835022661 7.233935646404498 4 1.9017679110296595 4.1807854980017805 5 1.0755407675597306 2.9555428513677464 6 0.6167355713400909 2.0337175092236706 7 0.4102411581543249 1.5782567267060894 8 0.3074277503165252 1.3516785857541957 9 0.21227062799224933 1.0499600953056276 >10 1.1863571289252035 11.772823308847602 >50 0.07553438941878456 2.847550671845136 >100 0.04073193566336196 4.19408589045709 >500 0.003457548380950295 1.3504061453562437 >1k 0.002247406447617678 1.8575804227017383 >5k 1.728774190475137E-4 0.6727802656207359 >10k+ 8.643870952375685E-5 0.5063191689723064 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10658 0.5057263137116458 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8694 0.41253373723109865 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5468 0.2594587618104034 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2590 0.12289652397383774 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2286 0.10847160378540273 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2260 0.1072378935061287 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2174 0.103157159505453 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2160 0.10249285397045929 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 9.490079071338823E-5 4 0.0 0.0 0.0 0.0 9.490079071338823E-5 5 0.0 0.0 0.0 0.0 9.490079071338823E-5 6 0.0 0.0 0.0 0.0 9.490079071338823E-5 7 0.0 0.0 0.0 0.0 9.490079071338823E-5 8 0.0 0.0 0.0 0.0 9.490079071338823E-5 9 0.0 0.0 0.0 0.0 9.490079071338823E-5 10-11 0.0 0.0 0.0 0.0 9.490079071338823E-5 12-13 0.0 0.0 0.0 0.0 1.6607638374842938E-4 14-15 0.0 0.0 0.0 0.0 1.8980158142677646E-4 16-17 0.0 0.0 0.0 0.0 1.8980158142677646E-4 18-19 0.0 0.0 0.0 4.7450395356694114E-5 1.8980158142677646E-4 20-21 0.0 0.0 0.0 9.490079071338823E-5 2.135267791051235E-4 22-23 0.0 0.0 0.0 2.3725197678347058E-4 2.3725197678347055E-4 24-25 0.0 0.0 0.0 5.931299419586764E-4 2.3725197678347055E-4 26-27 0.0 0.0 0.0 0.0012574354769523941 2.8470237214016467E-4 28-29 0.0 0.0 0.0 0.0035587796517520583 2.8470237214016467E-4 30-31 0.0 0.0 0.0 0.011838873641495181 2.8470237214016467E-4 32-33 0.0 0.0 0.0 0.028327886027946385 2.8470237214016467E-4 34-35 0.0 0.0 0.0 0.05226661048539856 2.8470237214016467E-4 36-37 0.0 0.0 0.0 0.09257572134091022 2.8470237214016467E-4 38-39 0.0 0.0 0.0 0.15105833361803572 2.8470237214016467E-4 40-41 0.0 0.0 0.0 0.21352677910512352 2.8470237214016467E-4 42-43 0.0 0.0 0.0 0.2754020946502526 2.8470237214016467E-4 44-45 0.0 0.0 0.0 0.3516311547907817 3.084275698185117E-4 46-47 0.0 0.0 0.0 0.43822812631674846 3.3215276749685876E-4 48-49 0.0 0.0 0.0 0.5314681531926524 3.3215276749685876E-4 50-51 0.0 0.0 0.0 0.6342457095352518 3.3215276749685876E-4 52-53 0.0 0.0 0.0 0.7295972790045286 3.796031628535529E-4 54-55 0.0 0.0 0.0 0.8328730644983734 3.796031628535529E-4 56-57 0.0 0.0 0.0 0.9521159080297457 3.796031628535529E-4 58-59 0.0 0.0 0.0 1.0784763108646223 4.0332836053189996E-4 60-61 0.0 0.0 0.0 1.2148724723174393 4.745039535669411E-4 62-63 0.0 0.0 0.0 1.3464524186415523 4.745039535669411E-4 64-65 0.0 0.0 0.0 1.4840585651759652 4.745039535669411E-4 66-67 2.3725197678347057E-5 0.0 0.0 1.6446544282606963 4.745039535669411E-4 68-69 4.7450395356694114E-5 0.0 0.0 1.8186075776383368 4.745039535669411E-4 70-71 4.7450395356694114E-5 0.0 0.0 1.9984208508425292 4.745039535669411E-4 72-73 4.7450395356694114E-5 0.0 0.0 2.1821250564659707 5.456795466019823E-4 74-75 4.7450395356694114E-5 0.0 0.0 2.3696490189156254 6.405803373153705E-4 76-77 4.7450395356694114E-5 0.0 0.0 2.565334449366632 6.643055349937175E-4 78-79 4.7450395356694114E-5 0.0 0.0 2.782918237274753 7.117559303504117E-4 80-81 4.7450395356694114E-5 0.0 0.0 3.016872411580933 7.117559303504117E-4 82-83 4.7450395356694114E-5 0.0 0.0 3.253863411189942 7.117559303504117E-4 84-85 4.7450395356694114E-5 0.0 0.0 3.4910204871826993 7.117559303504117E-4 86-87 4.7450395356694114E-5 0.0 0.0 3.742721109352283 7.354811280287588E-4 88-89 4.7450395356694114E-5 0.0 0.0 4.011646225036348 7.592063257071058E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5135 0.0 48.07863 1 GTATCAA 8830 0.0 39.316574 1 TATCAAC 10975 0.0 31.78142 2 ATCAACG 11065 0.0 31.39408 3 TCAACGC 11195 0.0 31.071968 4 CAACGCA 11355 0.0 30.550444 5 AACGCAG 11745 0.0 29.616917 6 ACGCAGA 13260 0.0 25.885777 7 CGCAGAG 13230 0.0 25.872606 8 GTACATG 7910 0.0 24.148241 1 TACATGG 7720 0.0 24.129622 2 TGGTATC 2690 0.0 23.318651 2 GCAGAGT 14380 0.0 23.24149 9 GTGGTAT 2925 0.0 23.182724 1 GTGTAGC 1160 0.0 23.053242 1 GAGTACT 8475 0.0 22.688461 12-13 ACATGGG 8095 0.0 22.424784 3 GTACTTT 8755 0.0 21.813108 14-15 AGAGTAC 12290 0.0 21.081264 10-11 AGTACTT 8705 0.0 20.723793 12-13 >>END_MODULE