FastQCFastQC Report
Thu 26 May 2016
SRR938121_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938121_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2107464
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101910.4835669790800696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT89830.4262469014891832No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56210.2667186722999776No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA24900.11815148443816834No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23370.11089157394859414No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA22910.10870885576218621No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC22840.10837670299468935No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22710.10775984785505234No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA22390.10624143520363812No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48200.053.9106521
GTATCAA82750.038.405411
TATCAAC104400.030.1225532
ATCAACG104450.030.0590843
TCAACGC105800.029.6292344
GTAAGGT11100.029.0969924
CAACGCA108100.028.9548855
AACGCAG111250.028.0923486
TAAGGTG12300.027.4167145
AGGTAAG10850.025.8319132
ACGCAGA127150.024.4673587
CGCAGAG127500.024.176688
GTACATG75900.024.096461
TACATGG74000.023.816422
GTGGTAT24700.022.6944331
GAGTACT85850.022.65668712-13
ACATGGG75100.022.3895873
TGGTATC24300.022.286042
TAAGGTA6250.022.0384034
GTACTTT90000.021.55918314-15
Produced by FastQC (version 0.11.5)