##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938121_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2107464 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.492886236728125 34.0 31.0 34.0 31.0 34.0 2 32.71115141231357 34.0 31.0 34.0 31.0 34.0 3 32.7707192151325 34.0 31.0 34.0 31.0 34.0 4 36.23271097394783 37.0 35.0 37.0 35.0 37.0 5 36.19535090516374 37.0 35.0 37.0 35.0 37.0 6 36.233320237024216 37.0 37.0 37.0 35.0 37.0 7 36.19563276051216 37.0 37.0 37.0 35.0 37.0 8 36.186311130344336 37.0 37.0 37.0 35.0 37.0 9 37.88925220074934 39.0 38.0 39.0 35.0 39.0 10-11 37.87887669730064 39.0 38.0 39.0 35.0 39.0 12-13 37.82648932555906 39.0 38.0 39.0 35.0 39.0 14-15 39.14007593961273 40.5 38.5 41.0 36.0 41.0 16-17 39.08937353141027 40.0 38.0 41.0 36.0 41.0 18-19 39.11608620598027 40.0 38.5 41.0 36.0 41.0 20-21 39.03863268838756 40.0 38.5 41.0 35.5 41.0 22-23 38.89935771144845 40.0 38.0 41.0 35.0 41.0 24-25 38.86358817991672 40.0 38.0 41.0 35.0 41.0 26-27 38.82460459585549 40.0 38.0 41.0 35.0 41.0 28-29 38.763810437568566 40.0 38.0 41.0 35.0 41.0 30-31 38.565941577175224 40.0 38.0 41.0 34.5 41.0 32-33 38.38290476136247 40.0 38.0 41.0 34.0 41.0 34-35 38.187037121393296 40.0 38.0 41.0 34.0 41.0 36-37 38.05297836641574 40.0 38.0 41.0 33.5 41.0 38-39 38.042584120060894 40.0 38.0 41.0 33.0 41.0 40-41 37.97958660266558 40.0 38.0 41.0 33.0 41.0 42-43 37.819334517695204 40.0 37.5 41.0 33.0 41.0 44-45 37.61069441755589 40.0 37.0 41.0 33.0 41.0 46-47 37.28179200214096 40.0 37.0 41.0 32.0 41.0 48-49 36.92607679182183 40.0 36.5 41.0 31.0 41.0 50-51 36.61872682048187 40.0 36.0 41.0 31.0 41.0 52-53 36.19280685221669 39.0 35.0 41.0 30.0 41.0 54-55 35.95680044831134 39.0 35.0 41.0 29.0 41.0 56-57 35.699268884308346 39.0 35.0 40.0 29.0 41.0 58-59 35.341102623817065 38.0 35.0 40.0 28.0 41.0 60-61 34.97271934419757 38.0 34.0 40.0 27.5 41.0 62-63 34.5949620017234 37.0 34.0 40.0 26.5 41.0 64-65 34.18787509537529 36.5 34.0 39.5 26.0 41.0 66-67 33.75669169200518 36.0 33.5 39.0 26.0 41.0 68-69 33.289058792937865 35.5 33.0 39.0 25.0 40.0 70-71 32.776994292666444 35.0 32.5 38.0 23.5 40.0 72-73 32.36549283878634 35.0 32.0 37.0 23.0 39.5 74-75 31.919576087657962 35.0 32.0 37.0 21.5 39.0 76-77 30.643267453204423 33.5 30.0 35.5 20.5 37.5 78-79 31.369193969624156 34.5 31.5 36.0 21.0 38.0 80-81 31.22739819043172 35.0 31.5 35.5 20.0 37.0 82-83 30.87317719306237 34.5 31.0 35.0 19.5 37.0 84-85 30.50647104766677 34.0 31.0 35.0 18.0 36.0 86-87 30.088263192158916 34.0 30.5 35.0 13.0 36.0 88-89 29.62979984474231 34.0 30.0 35.0 7.0 35.5 90-91 29.28926472765371 34.0 30.0 35.0 3.5 35.0 92-93 28.88103070799786 34.0 29.0 35.0 2.0 35.0 94-95 28.46700821461244 34.0 29.0 35.0 2.0 35.0 96-97 27.877543578443095 34.0 28.5 35.0 2.0 35.0 98-99 27.189995890795764 33.0 27.0 35.0 2.0 35.0 100-101 24.758398719978135 31.0 21.0 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.13302329464055163 1101 2 0.15923701821631653 1101 3 0.14548530753726396 1101 4 0.055824450041299656 1101 5 0.06697702759147006 1101 6 0.07498610311249365 1101 7 0.13638727179802856 1101 8 0.09881579393763928 1101 9 0.012738467127817898 1101 10-11 0.12305918954891126 1101 12-13 0.174658120949843 1101 14-15 0.10897265825394697 1101 16-17 0.11565971268012731 1101 18-19 0.1535158733257802 1101 20-21 0.19699341805019088 1101 22-23 0.13708373959071452 1101 24-25 0.24371252829777035 1101 26-27 0.11525141100052139 1101 28-29 0.1714906595012522 1101 30-31 0.09702840418146508 1101 32-33 0.12019305223995502 1101 34-35 0.03470004000669746 1101 36-37 0.18431268343994844 1101 38-39 0.15982885238715028 1101 40-41 0.2133528105121485 1101 42-43 0.23878126856654092 1101 44-45 0.1989956755482396 1101 46-47 0.22501140250471252 1101 48-49 0.15367479310861398 1101 50-51 0.16631524063596714 1101 52-53 0.088888621213421 1101 54-55 0.14587415584448138 1101 56-57 0.18938134125797745 1101 58-59 0.25343113877632817 1101 60-61 0.22031639480596255 1101 62-63 0.17350129522144186 1101 64-65 0.18333502332107088 1101 66-67 -0.016599886186348556 1101 68-69 -0.03863585093476729 1101 70-71 -0.019982516035504716 1101 72-73 -0.10143800084630783 1101 74-75 -0.055350269348029 1101 76-77 0.06662108661065602 1101 78-79 0.15984729290051192 1101 80-81 0.3031477008270187 1101 82-83 0.2707032456534968 1101 84-85 0.19064513556282492 1101 86-87 0.1530738015311215 1101 88-89 -0.016520075061876582 1101 90-91 0.04177127040803086 1101 92-93 0.3548466771646126 1101 94-95 0.2370035380338784 1101 96-97 0.15273535866951704 1101 98-99 0.21425683756221048 1101 100-101 0.43540064580121296 1102 1 0.17124258830400407 1102 2 0.16406981187434155 1102 3 0.11933391840940999 1102 4 0.07725568238689107 1102 5 0.06055104216089546 1102 6 0.04920285056253704 1102 7 0.1253881258748848 1102 8 0.10161647368568794 1102 9 0.12154672248077958 1102 10-11 0.06384435640907782 1102 12-13 0.0940752199509447 1102 14-15 0.17576877982530448 1102 16-17 0.1883940710090357 1102 18-19 0.20211610180296447 1102 20-21 0.1368587601485345 1102 22-23 0.18473675911150877 1102 24-25 0.18081258342783713 1102 26-27 0.08816095067221141 1102 28-29 0.05582201755579774 1102 30-31 0.11644741490935928 1102 32-33 0.12479514697583483 1102 34-35 0.2266684107452832 1102 36-37 0.22779245504543866 1102 38-39 0.12956815485487994 1102 40-41 0.20422534845346263 1102 42-43 0.25924152454159355 1102 44-45 0.13535558240064205 1102 46-47 0.19017935287008214 1102 48-49 0.22068650062681172 1102 50-51 0.12149170593579584 1102 52-53 0.12467175492630389 1102 54-55 0.00900769306089444 1102 56-57 0.19735879631560493 1102 58-59 0.10232386145245442 1102 60-61 0.07097526886087735 1102 62-63 0.009074061484945162 1102 64-65 0.10237210203719371 1102 66-67 0.0589856576456711 1102 68-69 0.27442361978558694 1102 70-71 0.17988374059159895 1102 72-73 0.16483660682343526 1102 74-75 0.060635099225144984 1102 76-77 0.01211620576548711 1102 78-79 0.06202139127590556 1102 80-81 0.1406281895883872 1102 82-83 0.25419987662253973 1102 84-85 0.3215390021749265 1102 86-87 0.22352403385759345 1102 88-89 -0.0022490345709869075 1102 90-91 0.0897496709953991 1102 92-93 0.14755710790765875 1102 94-95 0.08349497685629714 1102 96-97 0.22877002912657218 1102 98-99 0.23873934087027138 1102 100-101 0.3396170625741881 1103 1 0.1535179434905558 1103 2 0.1620387489018995 1103 3 0.1131509197572882 1103 4 0.09621575867405596 1103 5 0.10845686898728246 1103 6 0.07177089299751316 1103 7 0.09849388280058236 1103 8 0.06892597360853614 1103 9 0.11500497166311874 1103 10-11 0.13656359073362978 1103 12-13 0.20003634718236896 1103 14-15 0.17747723357426537 1103 16-17 0.1751210927381166 1103 18-19 0.15094718794583883 1103 20-21 0.22148021820105868 1103 22-23 0.2613380131377312 1103 24-25 0.2783595850726428 1103 26-27 0.19480137050472734 1103 28-29 0.27844659874794075 1103 30-31 0.22788352854762906 1103 32-33 0.22170447516494818 1103 34-35 0.23032286377479494 1103 36-37 0.22134428645820492 1103 38-39 0.23690015832265487 1103 40-41 0.2740698194108475 1103 42-43 0.22479632722059506 1103 44-45 0.26690226180131305 1103 46-47 0.21938978657077968 1103 48-49 0.18187920311125794 1103 50-51 0.23050125302700764 1103 52-53 0.16176585265000654 1103 54-55 0.3017066240573314 1103 56-57 0.3691700517875489 1103 58-59 0.31068025568652047 1103 60-61 0.30606928675371137 1103 62-63 0.30755866215775995 1103 64-65 0.19089991847290833 1103 66-67 0.1613159403686666 1103 68-69 0.2090572228401939 1103 70-71 0.24751570370583664 1103 72-73 0.07799132344086246 1103 74-75 0.18539627591277608 1103 76-77 0.2608100704219716 1103 78-79 0.2125792581949142 1103 80-81 0.2992363188053737 1103 82-83 0.26374745460732285 1103 84-85 0.39660679757228934 1103 86-87 0.504295307203865 1103 88-89 0.35357031984062104 1103 90-91 0.43447521098555697 1103 92-93 0.41742878951097495 1103 94-95 0.2677273567461249 1103 96-97 0.2999247730033261 1103 98-99 0.6170284094435345 1103 100-101 0.8446248679893493 1104 1 0.20430808609356887 1104 2 0.14701131886611307 1104 3 0.16484572138885767 1104 4 0.05943435442761569 1104 5 0.07575714252742216 1104 6 0.05528384503330841 1104 7 0.13082386702951965 1104 8 0.060573254724587855 1104 9 0.12015164529614708 1104 10-11 0.11648382316673178 1104 12-13 0.14168445762679482 1104 14-15 0.2522387671129849 1104 16-17 0.18570366827265872 1104 18-19 0.21166093558397137 1104 20-21 0.2170778667787019 1104 22-23 0.29131270480035454 1104 24-25 0.21107997563102288 1104 26-27 0.2730132556095768 1104 28-29 0.15070895124842565 1104 30-31 0.15033959833285593 1104 32-33 0.10498721946780876 1104 34-35 0.24258241038098305 1104 36-37 0.26111576502628253 1104 38-39 0.23286651247074275 1104 40-41 0.25075394343967616 1104 42-43 0.32496122534451644 1104 44-45 0.2706373494405838 1104 46-47 0.3018115498058265 1104 48-49 0.28685869796734664 1104 50-51 0.35680243468171113 1104 52-53 0.3144020110904293 1104 54-55 0.36246218366737537 1104 56-57 0.41101450572319465 1104 58-59 0.4649263478221428 1104 60-61 0.4372635057820986 1104 62-63 0.4306866261427942 1104 64-65 0.5303270875467021 1104 66-67 0.5855851292993464 1104 68-69 0.6828957139048626 1104 70-71 0.6446261163381735 1104 72-73 0.5661118856702032 1104 74-75 0.5167029380457784 1104 76-77 0.5688599462300523 1104 78-79 0.7186668739726052 1104 80-81 0.6802180224393517 1104 82-83 0.715561057527669 1104 84-85 0.8656338018861796 1104 86-87 0.7749023893916913 1104 88-89 0.8673717261050875 1104 90-91 0.8009706213441419 1104 92-93 0.9408714836118257 1104 94-95 0.8838630459249082 1104 96-97 1.0348665887072102 1104 98-99 1.1181600971129413 1104 100-101 1.2573159417899014 1105 1 0.13782487660599685 1105 2 0.09227100566617707 1105 3 0.07804663110996302 1105 4 0.06946743157563162 1105 5 0.0957151336204376 1105 6 0.07699699975800911 1105 7 0.050414147470824844 1105 8 0.09649184344473838 1105 9 0.044642448769579346 1105 10-11 0.040343011044654986 1105 12-13 0.059776489692652035 1105 14-15 0.06193335315759185 1105 16-17 0.13741078886202018 1105 18-19 0.04023840587448291 1105 20-21 0.09205978428003192 1105 22-23 0.0909871534666351 1105 24-25 -0.006659519598514407 1105 26-27 0.13182488485708888 1105 28-29 -0.10901246546391263 1105 30-31 -0.015106416726830219 1105 32-33 0.1432640535640317 1105 34-35 0.17629097491592205 1105 36-37 0.22369782196746257 1105 38-39 0.021822739773618594 1105 40-41 -7.336876888643928E-4 1105 42-43 0.028135516742821665 1105 44-45 0.018664089861722744 1105 46-47 0.12553771242755118 1105 48-49 0.021898333477260223 1105 50-51 0.06455851051185846 1105 52-53 0.10353408654543017 1105 54-55 0.08766582467525552 1105 56-57 0.03422699266729268 1105 58-59 -0.01021889832766476 1105 60-61 -0.013137582839618744 1105 62-63 0.09891657182230773 1105 64-65 -0.0864021323997406 1105 66-67 0.08459595579450507 1105 68-69 0.04203172216508477 1105 70-71 0.04564163388693743 1105 72-73 -0.032659477668346426 1105 74-75 -0.02566861427976974 1105 76-77 0.00417348459126643 1105 78-79 0.13931522942742092 1105 80-81 0.10541221852131955 1105 82-83 0.25685274736425967 1105 84-85 0.24990658461793558 1105 86-87 0.3335814800067922 1105 88-89 0.2739680446631958 1105 90-91 0.16783852907849806 1105 92-93 0.26193691991652557 1105 94-95 0.05966541118209179 1105 96-97 0.3615221161123152 1105 98-99 0.3823584529437767 1105 100-101 0.5539713320555641 1106 1 -0.16476819376677554 1106 2 -0.07365325866223316 1106 3 0.02464980547009077 1106 4 -0.07830274566947537 1106 5 -0.11714741753004176 1106 6 -0.07589641287265891 1106 7 4.658455540180739E-4 1106 8 -0.09657125628811514 1106 9 -0.12425780476581849 1106 10-11 -0.06602372167476034 1106 12-13 -0.08460290150765815 1106 14-15 -0.21473890226113213 1106 16-17 -0.20559536004122236 1106 18-19 -0.09139765237953412 1106 20-21 -0.004228055165413025 1106 22-23 -0.08056509902586839 1106 24-25 -0.030601622849225407 1106 26-27 -0.07476775849078621 1106 28-29 -0.0805585294980844 1106 30-31 0.009157656486671328 1106 32-33 -0.03358921957750738 1106 34-35 -0.019972153228543732 1106 36-37 -0.0587717004068935 1106 38-39 -0.07564909423449251 1106 40-41 -0.03268739887117533 1106 42-43 -0.10223467215614335 1106 44-45 -0.08589554922567544 1106 46-47 -0.15413828456286893 1106 48-49 -0.29969319764941105 1106 50-51 -0.20688471027659716 1106 52-53 -0.24865628744586132 1106 54-55 -0.6922737666142922 1106 56-57 -0.41911671005532725 1106 58-59 -0.4349778780784419 1106 60-61 -0.383277144271446 1106 62-63 -0.31519166707855817 1106 64-65 -0.5741127260621681 1106 66-67 -0.7034230825403327 1106 68-69 -0.8769900675881033 1106 70-71 -0.7744099068265307 1106 72-73 -0.7116678654750039 1106 74-75 -0.6255400176902697 1106 76-77 -0.6522835017953987 1106 78-79 -0.5602686126992644 1106 80-81 -0.5426468597769265 1106 82-83 -0.7103166305276751 1106 84-85 -0.5383276674569011 1106 86-87 -0.3615191324166531 1106 88-89 -0.3851804957750815 1106 90-91 -0.4283774306790882 1106 92-93 -0.49030995831460444 1106 94-95 -0.5573687563384127 1106 96-97 -0.49376631495825407 1106 98-99 -0.3111861131006819 1106 100-101 -0.19308149441113187 1107 1 0.0445286002177383 1107 2 0.07800026846246055 1107 3 0.04012617578779043 1107 4 0.03209151428097812 1107 5 0.08732342472298882 1107 6 0.08077118312132825 1107 7 0.06995701223645057 1107 8 -0.013306599015436404 1107 9 0.03083013948437241 1107 10-11 0.013100209240604954 1107 12-13 0.10370266049586974 1107 14-15 0.08975693357461978 1107 16-17 0.09028966456548204 1107 18-19 0.049082760716693485 1107 20-21 0.14043126039934606 1107 22-23 0.1265990525208025 1107 24-25 0.03320120091387935 1107 26-27 0.017867138282234407 1107 28-29 0.09946547744198853 1107 30-31 0.1092374066900419 1107 32-33 0.1006716602047888 1107 34-35 0.16346201416582318 1107 36-37 0.13048703619103463 1107 38-39 0.09573298743413972 1107 40-41 0.11040751492451051 1107 42-43 0.13691870656275285 1107 44-45 0.0396792929421963 1107 46-47 -0.03418218811425078 1107 48-49 0.12276620773668157 1107 50-51 0.06404040436432012 1107 52-53 0.039277257053562664 1107 54-55 0.09696943343222131 1107 56-57 0.06794271408106312 1107 58-59 0.19794908948558287 1107 60-61 0.1347430037085786 1107 62-63 0.19046617589133064 1107 64-65 0.10545490747337283 1107 66-67 0.0973570816026168 1107 68-69 0.17997257959462587 1107 70-71 0.3062988956226391 1107 72-73 0.11080574592779868 1107 74-75 0.09972329512826761 1107 76-77 0.14384501066270516 1107 78-79 0.28048511269556187 1107 80-81 0.29729094079598184 1107 82-83 0.3758763289376894 1107 84-85 0.4747808628262753 1107 86-87 0.44746684866027664 1107 88-89 0.5438241168817015 1107 90-91 0.5163285150097146 1107 92-93 0.6892295292944333 1107 94-95 0.6306550393536838 1107 96-97 0.5918825581607301 1107 98-99 0.667330050030241 1107 100-101 0.6006121395329771 1108 1 0.0974993484807598 1108 2 0.04558968976665767 1108 3 0.10607427904162137 1108 4 -0.002410649769878148 1108 5 0.014329916773348828 1108 6 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-0.6088942599585323 2316 90-91 -0.6796364203990457 2316 92-93 -0.7710335221413338 2316 94-95 -0.7558851961221151 2316 96-97 -0.7533591415328402 2316 98-99 -0.6548308165415619 2316 100-101 -0.551218286600065 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 4.0 3 7.0 4 9.0 5 37.0 6 69.0 7 187.0 8 464.0 9 833.0 10 1503.0 11 2366.0 12 3553.0 13 5203.0 14 7003.0 15 9890.0 16 12753.0 17 17762.0 18 18036.0 19 12971.0 20 10957.0 21 10900.0 22 11202.0 23 11998.0 24 13669.0 25 16156.0 26 19237.0 27 23254.0 28 28783.0 29 34760.0 30 43357.0 31 53387.0 32 66677.0 33 88053.0 34 122359.0 35 187918.0 36 312803.0 37 504637.0 38 409761.0 39 44941.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.49597430845794 18.51001962548352 14.882484350859611 23.111521715198933 2 16.02017518507287 19.52430376106415 39.56247867373026 24.89304238013272 3 19.74977518679071 23.20209936387946 29.532174981789538 27.515950467540296 4 13.409813880569251 16.36450254903524 35.737502514870954 34.48818105552455 5 14.037155557580105 36.86169728166175 33.86914319770112 15.23200396305702 6 33.354116606499566 35.409952435723696 16.34348202389222 14.892448933884516 7 29.414784783986818 32.52753071938596 19.50163798764771 18.556046508979513 8 27.15352268661617 34.62020997848589 18.713006309983516 19.51326102491442 9 26.395226844916593 16.590120871022982 19.671613079864652 37.343039204195776 10-11 25.695053391184857 26.17883389704403 26.144645887189533 21.981466824581585 12-13 26.52348984371738 23.40092167647941 26.90869215322302 23.166896326580193 14-15 23.114653441292475 24.706970083474737 25.451134633853766 26.727241841379023 16-17 22.559832746370272 27.986892265468637 26.37672969046121 23.076545297699884 18-19 22.640742617667488 26.240305884228626 28.793587933174656 22.325363564929223 20-21 23.034841876302515 25.362615921315857 29.359267821419486 22.24327438096214 22-23 22.97215990403632 25.264915557276424 29.214994894337458 22.547929644349797 24-25 22.82477272576287 25.39803242531198 29.19509472962931 22.58210011929584 26-27 22.210974236893012 25.836218892438882 29.16956171774296 22.78324515292514 28-29 22.415535270497102 26.180774272377327 29.050006820999187 22.353683636126384 30-31 22.462091879149536 25.73087843967916 28.839472465484583 22.96755721568672 32-33 22.19658568817091 25.701979048163597 29.008340638756984 23.09309462490851 34-35 22.959219509787452 25.828893552517552 28.78249867352353 22.429388264171468 36-37 23.04941376773306 25.435056540089597 28.674809249413435 22.84072044276391 38-39 22.520558347027034 25.792785150294538 28.68752854914011 22.999127953538316 40-41 23.017763778899777 25.631171538089546 28.684224638647503 22.666840044363177 42-43 22.690451791299008 25.607652214682076 28.566422192447583 23.135473801571337 44-45 22.610655194151505 25.94279670316243 28.664853859956768 22.781694242729298 46-47 23.095791909138185 26.229155041319803 27.98202009619144 22.693032953350567 48-49 22.82186077674399 26.224694704156278 27.912766244168346 23.040678274931388 50-51 22.98710677857368 26.52868566200894 27.819075438536554 22.66513212088083 52-53 23.370553423451124 26.186710662673242 27.6829402542582 22.759795659617435 54-55 22.898730417221834 26.410273200396304 27.8445563008431 22.84644008153876 56-57 22.836546674106888 26.56370405378218 27.72872988577741 22.871019386333526 58-59 22.942432231345354 26.234445760402075 27.549604643305887 23.273517364946684 60-61 22.60370758409159 26.52562511150843 27.81312041391929 23.05754689048069 62-63 22.68942672330346 26.504628311563096 27.95203144632601 22.85391351880744 64-65 22.415068537351054 26.63770294534094 27.78790052878721 23.1593279885208 66-67 22.369231455436488 26.60773802067319 27.727662251881885 23.295368272008442 68-69 22.75460637668396 26.29187566638307 27.9495117105481 23.004006246384865 70-71 22.683875027046728 26.46325156681206 27.42224778216852 23.4306256239727 72-73 22.73089836884521 26.561568785991124 27.373255248962735 23.33427759620093 74-75 22.551346072815477 26.932132648529226 27.359779336681434 23.15674194197386 76-77 22.74147980700975 26.53181738810248 27.52488298732505 23.20181981756272 78-79 22.821386272790427 26.70816678244563 27.49188123735447 22.978565707409473 80-81 22.952942493916858 26.756352658930354 27.23863847733579 23.05206636981699 82-83 22.79636159772162 26.832682985968674 27.333545752986943 23.03740966332276 84-85 22.855058365758754 26.836860586504695 27.234649330929106 23.07343171680744 86-87 22.95192386852511 26.914087791485436 26.965097075412363 23.168891264577088 88-89 22.85893038217041 27.06436601733933 26.778595875704582 23.298107724785677 90-91 22.748722513700148 27.02716873732775 26.947736587046293 23.276372161925806 92-93 22.861842495481522 26.702798681064433 27.104182908595526 23.331175914858523 94-95 22.645155504435664 26.519859888472592 27.269504959515324 23.565479647576424 96-97 22.811350514172485 26.284221225131247 27.305140206428202 23.59928805426807 98-99 22.622426765059807 26.546266033488592 26.942168407147165 23.889138794304436 100-101 22.65611654576306 26.3627279042489 27.22663352731055 23.75452202267749 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1081.0 1 721.5 2 514.5 3 707.5 4 585.0 5 518.0 6 1090.0 7 2520.0 8 4033.5 9 4492.0 10 3570.0 11 2446.0 12 2239.5 13 2390.5 14 2571.5 15 2683.5 16 2758.0 17 2956.5 18 3192.5 19 3459.0 20 3759.0 21 4285.0 22 5150.5 23 6200.0 24 7512.5 25 9292.5 26 11680.0 27 14530.5 28 16815.5 29 19030.0 30 21709.0 31 25109.5 32 30850.0 33 33772.5 34 33761.5 35 35444.0 36 38727.0 37 42341.0 38 45210.5 39 48001.5 40 51579.0 41 56067.0 42 59044.0 43 61780.5 44 65098.0 45 68930.0 46 71605.0 47 75655.5 48 78355.5 49 77236.0 50 77930.0 51 78054.5 52 77860.0 53 79937.0 54 82717.0 55 79134.0 56 72813.0 57 68201.5 58 64383.5 59 57905.0 60 46629.0 61 38400.5 62 32336.5 63 24276.5 64 17291.5 65 13053.5 66 9445.5 67 6843.5 68 5192.5 69 3878.0 70 2884.5 71 1881.0 72 1232.0 73 926.0 74 627.0 75 405.5 76 298.0 77 188.5 78 94.0 79 51.0 80 28.0 81 16.5 82 14.0 83 7.0 84 2.0 85 1.5 86 1.0 87 0.5 88 1.0 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01523157690949881 3 0.007544612861714364 4 0.0 5 0.0 6 0.0 7 0.0 8 4.745039535669411E-4 9 5.219543489236352E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.8470237214016467E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.004650138744956023 26-27 0.019929166049811528 28-29 7.117559303504117E-5 30-31 0.0 32-33 5.456795466019823E-4 34-35 0.017936249444830375 36-37 0.0031080008958634642 38-39 0.016678813967877983 40-41 0.01475707295593187 42-43 0.007236185291895852 44-45 0.0023962449655130524 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 2.3725197678347055E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 2.3725197678347057E-5 84-85 0.0030368253028284233 86-87 2.1352677910512353E-4 88-89 4.7450395356694114E-5 90-91 2.3725197678347055E-4 92-93 3.3215276749685876E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2107464.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.88031562616098 #Duplication Level Percentage of deduplicated Percentage of total 1 75.07857178045185 39.701785725116764 2 13.884952837929553 14.684813770481492 3 4.712333194228767 7.475689999393579 4 2.070910178880374 4.3804153557049474 5 1.1115517201083935 2.9389602897066998 6 0.7000848804748073 2.22124256667666 7 0.45128431208810504 1.6704839802247753 8 0.33208725564201624 1.4048703115020342 9 0.24599477635278413 1.1707453274329898 >10 1.2770066044527866 12.263200880320477 >50 0.08405609723950934 3.062158346036814 >100 0.044436711451728846 4.386848272791045 >500 0.0037686043917043227 1.3399543117204122 >1k 0.002691860279788787 2.1223376915347756 >5k 1.7945735198591914E-4 0.6929423784831734 >10k+ 8.972867599295957E-5 0.4835507928733237 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10191 0.4835669790800696 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8983 0.4262469014891832 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5621 0.2667186722999776 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2490 0.11815148443816834 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2337 0.11089157394859414 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2291 0.10870885576218621 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2284 0.10837670299468935 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2271 0.10775984785505234 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2239 0.10624143520363812 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 2.3725197678347057E-5 14-15 0.0 0.0 0.0 0.0 7.117559303504117E-5 16-17 0.0 0.0 0.0 0.0 9.490079071338823E-5 18-19 0.0 0.0 0.0 0.0 9.490079071338823E-5 20-21 0.0 0.0 0.0 4.7450395356694114E-5 1.4235118607008234E-4 22-23 0.0 0.0 0.0 2.135267791051235E-4 1.4235118607008234E-4 24-25 0.0 0.0 0.0 5.219543489236352E-4 1.4235118607008234E-4 26-27 0.0 0.0 0.0 0.0011625346862390057 1.4235118607008234E-4 28-29 0.0 0.0 0.0 0.003487604058717017 1.4235118607008234E-4 30-31 0.0 0.0 0.0 0.011743972850781794 1.4235118607008234E-4 32-33 0.0 0.0 0.0 0.028588863202408204 1.4235118607008234E-4 34-35 0.0 0.0 0.0 0.052978366415748976 1.4235118607008234E-4 36-37 0.0 0.0 0.0 0.09357217964340078 1.4235118607008234E-4 38-39 0.0 0.0 0.0 0.15260047146712827 1.4235118607008234E-4 40-41 0.0 0.0 0.0 0.21535361932635622 1.4235118607008234E-4 42-43 0.0 0.0 0.0 0.2775373624413039 1.6607638374842938E-4 44-45 0.0 0.0 0.0 0.35426465173307825 2.135267791051235E-4 46-47 0.0 0.0 0.0 0.44157337918939543 2.609771744618176E-4 48-49 0.0 0.0 0.0 0.5351218336351178 2.8470237214016467E-4 50-51 0.0 0.0 0.0 0.6382078175475359 2.8470237214016467E-4 52-53 0.0 0.0 0.0 0.7335831122144909 3.3215276749685876E-4 54-55 0.0 0.0 0.0 0.8369063481036925 3.3215276749685876E-4 56-57 0.0 0.0 0.0 0.95650506960024 3.3215276749685876E-4 58-59 0.0 0.0 0.0 1.0837433047492153 3.3215276749685876E-4 60-61 0.0 0.0 0.0 1.2206139701555994 3.3215276749685876E-4 62-63 0.0 0.0 0.0 1.3526209700379224 3.796031628535529E-4 64-65 0.0 0.0 0.0 1.4912473000725042 3.796031628535529E-4 66-67 0.0 0.0 0.0 1.6526023694829426 3.796031628535529E-4 68-69 0.0 0.0 0.0 1.8280502063143191 3.796031628535529E-4 70-71 0.0 0.0 0.0 2.0099275717165277 4.0332836053189996E-4 72-73 0.0 0.0 0.0 2.194746861630851 4.982291512452882E-4 74-75 0.0 0.0 0.0 2.382958854813178 5.456795466019823E-4 76-77 0.0 0.0 0.0 2.5796407435666753 5.694047442803293E-4 78-79 0.0 0.0 0.0 2.7995733260449525 5.694047442803293E-4 80-81 0.0 0.0 0.0 3.036564325653961 5.694047442803293E-4 82-83 0.0 0.0 0.0 3.2785850671707797 6.168551396370234E-4 84-85 0.0 0.0 0.0 3.518684067675652 6.168551396370234E-4 86-87 0.0 0.0 0.0 3.7752720805669755 6.405803373153705E-4 88-89 0.0 4.7450395356694114E-5 0.0 4.049393014542597 6.643055349937175E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 4820 0.0 53.910652 1 GTATCAA 8275 0.0 38.40541 1 TATCAAC 10440 0.0 30.122553 2 ATCAACG 10445 0.0 30.059084 3 TCAACGC 10580 0.0 29.629234 4 GTAAGGT 1110 0.0 29.096992 4 CAACGCA 10810 0.0 28.954885 5 AACGCAG 11125 0.0 28.092348 6 TAAGGTG 1230 0.0 27.416714 5 AGGTAAG 1085 0.0 25.831913 2 ACGCAGA 12715 0.0 24.467358 7 CGCAGAG 12750 0.0 24.17668 8 GTACATG 7590 0.0 24.09646 1 TACATGG 7400 0.0 23.81642 2 GTGGTAT 2470 0.0 22.694433 1 GAGTACT 8585 0.0 22.656687 12-13 ACATGGG 7510 0.0 22.389587 3 TGGTATC 2430 0.0 22.28604 2 TAAGGTA 625 0.0 22.038403 4 GTACTTT 9000 0.0 21.559183 14-15 >>END_MODULE