FastQCFastQC Report
Thu 26 May 2016
SRR938120_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938120_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135028
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102290.47910378692925804No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86100.4032733996931188No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51580.2415893374700472No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA25800.12084150652825161No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23480.10997513849935457No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23290.10908522042802249No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.10262160496255787No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA48900.054.7339251
GTATCAA85450.039.9609871
TATCAAC106350.031.9784052
ATCAACG106350.031.8464743
TCAACGC108250.031.1556744
CAACGCA109750.030.5998275
AACGCAG114050.029.44066
ACGCAGA127650.026.1506297
CGCAGAG129300.025.7765148
GCAGAGT138700.023.3434549
GTACATG75000.022.9866921
GAGTACT85550.022.9775712-13
TAAGGTA5600.022.934784
TACATGG73750.022.8957332
TGGTATC21500.022.1232952
GTGGTAT23100.021.8536011
CAGAGTA131400.021.74315610-11
AGAGTAC120000.021.27338410-11
GTACTTT92050.021.19811214-15
ACATGGG77350.020.663213
Produced by FastQC (version 0.11.5)