FastQCFastQC Report
Thu 26 May 2016
SRR938120_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938120_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2135028
Sequences flagged as poor quality0
Sequence length101
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT101540.47559095243715777No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86380.40458485790350296No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56800.2660386655350656No Hit
GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA26740.12524425909168405No Hit
AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC24880.1165324295512752No Hit
CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA23540.1102561652587226No Hit
CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA23350.10936624718739052No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22870.1071180331124463No Hit
GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA21380.10013920192147364No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA50050.052.7750131
GTATCAA86350.039.447051
TATCAAC111400.030.4900512
ATCAACG112050.030.2241363
TCAACGC113350.029.95714
CAACGCA115000.029.5685775
AACGCAG118150.028.941036
ACGCAGA132750.025.471867
CGCAGAG133600.025.2742568
GTACATG77550.024.9940151
GTAAGGT10750.024.739724
TAAGGTG12150.024.2343085
TACATGG78300.024.0860772
ACATGGG78350.023.1579783
GAGTACT90950.023.02934612-13
ATAGCGT1652.435323E-623.0261466
TGGTATC23600.022.7459322
GCAGAGT147550.022.5306669
GTACTTT93450.022.08187314-15
GTGTAAG8950.021.7629851
Produced by FastQC (version 0.11.5)