##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938120_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2135028 Sequences flagged as poor quality 0 Sequence length 101 %GC 46 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.51375344960347 34.0 31.0 34.0 31.0 34.0 2 32.72848693319244 34.0 31.0 34.0 31.0 34.0 3 32.78323375618493 34.0 31.0 34.0 31.0 34.0 4 36.241597768272825 37.0 35.0 37.0 35.0 37.0 5 36.214358312865215 37.0 37.0 37.0 35.0 37.0 6 36.24731478931424 37.0 37.0 37.0 35.0 37.0 7 36.20618699145866 37.0 37.0 37.0 35.0 37.0 8 36.18790901102936 37.0 37.0 37.0 35.0 37.0 9 37.8738892417336 39.0 38.0 39.0 35.0 39.0 10-11 37.85620820897899 39.0 38.0 39.0 35.0 39.0 12-13 37.77236504626637 39.0 38.0 39.0 35.0 39.0 14-15 39.04598183255676 40.0 38.0 41.0 36.0 41.0 16-17 38.92893418727998 40.0 38.0 41.0 35.5 41.0 18-19 39.174800986216574 40.0 39.0 41.0 36.0 41.0 20-21 39.156044323540485 40.0 39.0 41.0 36.0 41.0 22-23 39.06241182785425 40.0 39.0 41.0 35.5 41.0 24-25 38.96354637971961 40.0 38.0 41.0 35.0 41.0 26-27 38.78399627545868 40.0 38.0 41.0 35.0 41.0 28-29 38.66679945181046 40.0 38.0 41.0 34.5 41.0 30-31 38.42319562085369 40.0 38.0 41.0 34.0 41.0 32-33 38.203662668592635 40.0 38.0 41.0 34.0 41.0 34-35 38.05495970076271 40.0 37.5 41.0 33.0 41.0 36-37 38.05829057042811 40.0 38.0 41.0 33.5 41.0 38-39 37.90915482138875 40.0 37.0 41.0 33.0 41.0 40-41 37.73292294058907 40.0 37.0 41.0 33.0 41.0 42-43 37.492064272693376 40.0 37.0 41.0 33.0 41.0 44-45 37.21062416980011 40.0 36.5 41.0 32.0 41.0 46-47 36.819009399408344 40.0 36.0 41.0 31.0 41.0 48-49 36.40487876505601 39.0 35.5 41.0 30.0 41.0 50-51 36.00952306011912 39.0 35.0 40.5 29.0 41.0 52-53 35.47723894019188 38.5 35.0 40.0 28.0 41.0 54-55 35.146176537263216 38.0 34.0 40.0 27.0 41.0 56-57 34.78126211927899 38.0 34.0 40.0 26.0 41.0 58-59 34.32053209606619 37.5 33.5 40.0 25.5 41.0 60-61 33.82580673415056 37.0 33.0 40.0 24.0 41.0 62-63 33.34244141060445 36.0 32.5 39.0 22.5 40.5 64-65 32.82948139321826 36.0 32.0 39.0 21.5 40.0 66-67 32.26666722872019 35.0 31.0 38.5 19.5 40.0 68-69 31.672727945488305 35.0 31.0 38.0 18.0 40.0 70-71 31.053795547412022 35.0 30.0 37.0 13.0 39.0 72-73 30.464259016743576 34.0 29.5 36.5 8.0 39.0 74-75 29.870671251149865 34.0 29.0 36.0 7.0 39.0 76-77 28.545671063798693 32.5 27.0 34.5 4.0 36.5 78-79 29.231311252124094 34.0 29.0 35.0 2.0 37.0 80-81 28.917819813135942 34.0 29.0 35.0 2.0 37.0 82-83 28.361467156402632 33.5 27.5 35.0 2.0 36.0 84-85 27.76969458948548 33.0 26.5 35.0 2.0 36.0 86-87 27.132002718465518 33.0 25.5 35.0 2.0 35.0 88-89 26.38900660787587 32.0 24.5 34.5 2.0 35.0 90-91 25.83757683739979 32.0 23.5 34.0 2.0 35.0 92-93 25.014827206013223 31.5 19.0 34.0 2.0 35.0 94-95 24.18047046689786 31.0 12.0 34.0 2.0 35.0 96-97 23.120994197734177 31.0 2.0 34.0 2.0 35.0 98-99 21.68065781807077 30.0 2.0 34.0 2.0 35.0 100-101 17.353471242531715 23.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.0813687018928988 1101 2 0.09083453049583312 1101 3 0.08107330107671373 1101 4 0.020161692575847212 1101 5 0.05693354018777086 1101 6 0.05323430548153851 1101 7 0.014435226075939056 1101 8 -0.02274061347233669 1101 9 0.006971200892195384 1101 10-11 0.019060307869132487 1101 12-13 0.12415196870949075 1101 14-15 0.05579145761436877 1101 16-17 0.23460884842162955 1101 18-19 0.1619488585732327 1101 20-21 0.11223439170441196 1101 22-23 0.1615028704624777 1101 24-25 0.1398156198805225 1101 26-27 0.16335113377817834 1101 28-29 0.17378089949308873 1101 30-31 0.22934504130380873 1101 32-33 0.2331727056618078 1101 34-35 1.0791340343985212E-4 1101 36-37 0.06916602573968333 1101 38-39 0.15525291321565504 1101 40-41 0.177888391296392 1101 42-43 0.24930869615435824 1101 44-45 0.17640533527295332 1101 46-47 0.2791866298987884 1101 48-49 0.36564687823121034 1101 50-51 0.06136699722728878 1101 52-53 0.022181182153083512 1101 54-55 -0.026546156754235994 1101 56-57 0.03654839648763897 1101 58-59 0.19683377913901268 1101 60-61 0.04036662180796213 1101 62-63 -0.023151959085488727 1101 64-65 -0.14942987573911637 1101 66-67 -0.06355124008356938 1101 68-69 -0.07102765912470588 1101 70-71 0.029919944976331436 1101 72-73 -0.0803010281945511 1101 74-75 -0.19466805461286896 1101 76-77 -0.2410468738174103 1101 78-79 -0.29907100868427605 1101 80-81 -0.19676996831924853 1101 82-83 -0.08413694695743956 1101 84-85 -0.1372846369502092 1101 86-87 -0.27229120495830017 1101 88-89 -0.29999392674383785 1101 90-91 -0.30319330897424024 1101 92-93 -0.6719911707820074 1101 94-95 -0.9129379824165831 1101 96-97 -0.5733825757427518 1101 98-99 -0.5183839560369456 1101 100-101 -0.8523055436658318 1102 1 0.11130594810921934 1102 2 0.11641328913106719 1102 3 0.13512724823697653 1102 4 0.10287276149954039 1102 5 0.10837054909985966 1102 6 0.03036872514087463 1102 7 0.08134051532341857 1102 8 0.11215760938269881 1102 9 0.07716710871268617 1102 10-11 0.1644750629184344 1102 12-13 0.1568096441827862 1102 14-15 0.22381603178511966 1102 16-17 0.16709909258131006 1102 18-19 0.2397160894966035 1102 20-21 0.18779073827403892 1102 22-23 0.21115152837526097 1102 24-25 0.24665195906736415 1102 26-27 0.1810672124577053 1102 28-29 0.11985615129923843 1102 30-31 0.0871549869875139 1102 32-33 -0.11579963521252523 1102 34-35 0.06773170710696519 1102 36-37 0.19190042957873743 1102 38-39 0.16087834168161663 1102 40-41 0.12359054812811365 1102 42-43 0.18580674498630145 1102 44-45 0.0033288178442774097 1102 46-47 0.18730507107370897 1102 48-49 0.19141218435365914 1102 50-51 0.2223792315783939 1102 52-53 0.10750325671934746 1102 54-55 0.1303158903902002 1102 56-57 0.171096595612255 1102 58-59 0.054072876328277175 1102 60-61 -0.0867809814193734 1102 62-63 0.1365656488879381 1102 64-65 0.2383021550577027 1102 66-67 0.20241933419264768 1102 68-69 0.1765689764287366 1102 70-71 0.043991426282033785 1102 72-73 0.022091789397602923 1102 74-75 4.072235604155594E-4 1102 76-77 -0.002369581194933801 1102 78-79 -0.09279826702947247 1102 80-81 -0.2120952069418287 1102 82-83 -0.18290626088456108 1102 84-85 -0.23667687446537045 1102 86-87 -0.34223530662997703 1102 88-89 -0.2059245548090196 1102 90-91 -0.5465244011010135 1102 92-93 -0.46262688243101024 1102 94-95 -0.5034932743007765 1102 96-97 -0.6402001942630271 1102 98-99 -0.7878435615845909 1102 100-101 -1.2930084915116353 1103 1 0.12533592608748023 1103 2 0.0941008037192006 1103 3 0.035302559535040245 1103 4 0.04605211180754054 1103 5 -0.019564583182784645 1103 6 -0.06201297144626494 1103 7 0.004076437141179667 1103 8 -0.026212731294592118 1103 9 0.00491018505809393 1103 10-11 0.08103906295902874 1103 12-13 0.13265892720777828 1103 14-15 0.13155357732546236 1103 16-17 0.10972066881001297 1103 18-19 0.054493994691505065 1103 20-21 0.17537458832325825 1103 22-23 0.18596162894457535 1103 24-25 0.1858492565840777 1103 26-27 0.170163407872721 1103 28-29 0.2695316604884894 1103 30-31 0.24240062577012367 1103 32-33 0.1459440652512498 1103 34-35 0.15776560231596903 1103 36-37 0.18429316240533922 1103 38-39 0.17065746466844445 1103 40-41 0.2792268951582244 1103 42-43 0.1358477186109539 1103 44-45 0.07639582684578983 1103 46-47 0.12136113009602667 1103 48-49 0.10393628648581199 1103 50-51 0.12932089934445656 1103 52-53 0.030467133965920823 1103 54-55 0.0026367481216595934 1103 56-57 0.13117395077112803 1103 58-59 0.29380714397864693 1103 60-61 0.1519945287847051 1103 62-63 0.07569507987465585 1103 64-65 0.1910288673239151 1103 66-67 0.008641236180700673 1103 68-69 -0.023916871814090968 1103 70-71 -0.22536423492824653 1103 72-73 -0.37211735621987074 1103 74-75 -0.3509005073887508 1103 76-77 -0.42401316901295516 1103 78-79 -0.5017485303769789 1103 80-81 -0.3824357573837851 1103 82-83 -0.4504159524787532 1103 84-85 -0.49586181646802885 1103 86-87 -0.64731603608735 1103 88-89 -0.8164121536277591 1103 90-91 -0.8730013543885455 1103 92-93 -0.8032014265201504 1103 94-95 -0.7803197085044964 1103 96-97 -1.0689201680804992 1103 98-99 -1.2161403527028263 1103 100-101 -1.8908159586058826 1104 1 0.10740482265178031 1104 2 0.04644603688458915 1104 3 0.1239873403646925 1104 4 0.04775444544426222 1104 5 -0.00710868270886067 1104 6 0.05860083963964513 1104 7 0.020022566200800895 1104 8 0.002559903698205801 1104 9 0.011732017324753485 1104 10-11 0.025570337180873537 1104 12-13 0.11630686259649536 1104 14-15 0.03790792277061428 1104 16-17 0.062143652212796496 1104 18-19 0.12441933763975044 1104 20-21 0.12300517687693713 1104 22-23 0.06185518586531913 1104 24-25 0.13951588327406483 1104 26-27 0.09127671917001123 1104 28-29 -0.06390485914651123 1104 30-31 -0.022212152811853514 1104 32-33 0.026761964100955993 1104 34-35 0.030561527290238644 1104 36-37 -0.020791174370714316 1104 38-39 0.018587133269782896 1104 40-41 0.19115498327868607 1104 42-43 0.09545364627943798 1104 44-45 0.038893174084599025 1104 46-47 0.06480726576502605 1104 48-49 0.05404707156132105 1104 50-51 0.04036657634713947 1104 52-53 -0.06390891617301264 1104 54-55 -0.07208026865340145 1104 56-57 -0.09979304347199047 1104 58-59 -0.08471718295304953 1104 60-61 -0.15370870949465854 1104 62-63 -0.08114649638583415 1104 64-65 -0.12890903342929505 1104 66-67 -0.15177721151118106 1104 68-69 -0.1436273621356854 1104 70-71 -0.4143287964421454 1104 72-73 -0.4199636746439843 1104 74-75 -0.43985759430486127 1104 76-77 -0.5142570403835407 1104 78-79 -0.439593524523481 1104 80-81 -0.3971280286737091 1104 82-83 -0.4784583120578567 1104 84-85 -0.35869357384691014 1104 86-87 -0.5415443487581832 1104 88-89 -0.5300204159664332 1104 90-91 -0.860476596285519 1104 92-93 -0.8730847098345649 1104 94-95 -1.0562428033052278 1104 96-97 -1.2689162505260363 1104 98-99 -1.2149015162309276 1104 100-101 -1.7096708838731232 1105 1 0.13323223274736762 1105 2 0.07918472538919019 1105 3 0.09720784318682263 1105 4 0.07427635118399678 1105 5 0.0699716867300566 1105 6 0.01835058455130678 1105 7 0.07171197464160883 1105 8 0.018327139341579368 1105 9 0.09950174878340334 1105 10-11 0.12216655759539918 1105 12-13 0.07315779361204733 1105 14-15 0.07722315734721263 1105 16-17 0.022305384137482065 1105 18-19 0.04748733358746193 1105 20-21 0.03336608705762245 1105 22-23 0.12535124781105367 1105 24-25 0.06521964037717254 1105 26-27 0.10848755912745389 1105 28-29 0.02100531152830598 1105 30-31 0.10072199571190055 1105 32-33 0.20382592687671774 1105 34-35 0.2506739800613431 1105 36-37 0.282070593689582 1105 38-39 0.2582639060768628 1105 40-41 0.18608814603733492 1105 42-43 0.20568981120712948 1105 44-45 0.2095415854371936 1105 46-47 0.20651987563960006 1105 48-49 0.36602352606548294 1105 50-51 0.3053570554763141 1105 52-53 0.2616766492401936 1105 54-55 0.44020481771254794 1105 56-57 0.3715678858232536 1105 58-59 0.24630931892791352 1105 60-61 0.13151430279600618 1105 62-63 0.14389682994792707 1105 64-65 0.14687809955979247 1105 66-67 0.11579225398204329 1105 68-69 -0.045025819681796975 1105 70-71 -0.10123561933847114 1105 72-73 -0.11888115345185568 1105 74-75 -0.2684592778424033 1105 76-77 -0.12075278193285754 1105 78-79 -0.24798486144124965 1105 80-81 -0.22733647293975423 1105 82-83 -0.205438352817378 1105 84-85 -0.3884966984401572 1105 86-87 -0.5438466291250741 1105 88-89 -0.6556425493514659 1105 90-91 -0.7383923629750306 1105 92-93 -0.8087537512576333 1105 94-95 -0.755062319998153 1105 96-97 -0.8550100447568951 1105 98-99 -1.0290735281474888 1105 100-101 -1.7284825681479425 1106 1 0.15651729107337076 1106 2 0.15990102130258776 1106 3 0.16465984779365073 1106 4 0.11656610400508072 1106 5 0.15215255628475433 1106 6 0.18787082710525738 1106 7 0.1645076023829617 1106 8 0.1127022092451142 1106 9 0.11461073784973053 1106 10-11 0.12358994482625718 1106 12-13 0.09108408429590753 1106 14-15 0.2020587895623862 1106 16-17 0.24168347689683145 1106 18-19 0.1763743483057496 1106 20-21 0.3051510531865276 1106 22-23 0.3476120114448875 1106 24-25 0.263778191582837 1106 26-27 0.25703220519638137 1106 28-29 0.19024329492072667 1106 30-31 0.22215842656559204 1106 32-33 0.26036504414236106 1106 34-35 0.33085560835285577 1106 36-37 0.2815272353572311 1106 38-39 0.2146599666911655 1106 40-41 0.17271918834824618 1106 42-43 0.2877701708060627 1106 44-45 0.15624863745751583 1106 46-47 0.11126364326663207 1106 48-49 0.06457678727348082 1106 50-51 0.05809160641597089 1106 52-53 0.0971033102238934 1106 54-55 -0.009856470689065588 1106 56-57 0.044142724176040815 1106 58-59 -0.08328285248010303 1106 60-61 -0.1406753481548364 1106 62-63 0.025610592708162017 1106 64-65 -0.03288660134374055 1106 66-67 -0.26791621575766555 1106 68-69 -0.5020196500330805 1106 70-71 -0.6679353107048946 1106 72-73 -0.435186739991682 1106 74-75 -0.3116169681387362 1106 76-77 -0.4696353077102593 1106 78-79 -0.3336337023271909 1106 80-81 -0.4443574938790782 1106 82-83 -0.38437668051308904 1106 84-85 -0.6440394824299034 1106 86-87 -0.7045337091370492 1106 88-89 -0.567480581714161 1106 90-91 -0.9511802522055 1106 92-93 -0.9370497976645176 1106 94-95 -0.9590004209796739 1106 96-97 -1.1222637500224089 1106 98-99 -1.2813418035780195 1106 100-101 -1.6383765263660273 1107 1 -0.0038582161173081886 1107 2 -0.025081677804678293 1107 3 0.004833853076902983 1107 4 2.6989741452609906E-4 1107 5 -0.07253317741473353 1107 6 -0.020002858294070336 1107 7 0.08552294276451988 1107 8 0.05538210807882393 1107 9 -0.02224703548839102 1107 10-11 0.01338434315909609 1107 12-13 0.034212809984651926 1107 14-15 0.05233717421505446 1107 16-17 -0.029678033009439275 1107 18-19 -0.024911837557333172 1107 20-21 0.015154186924327462 1107 22-23 -0.005362017732238655 1107 24-25 0.00488186463621787 1107 26-27 -0.05738121532159823 1107 28-29 -0.043243388560320284 1107 30-31 -0.14790602389743412 1107 32-33 -0.06511621094879416 1107 34-35 0.12416833321837828 1107 36-37 -0.007776381834879942 1107 38-39 -0.029627145292941748 1107 40-41 0.008054800466645418 1107 42-43 0.024833352140277043 1107 44-45 0.02980558386686738 1107 46-47 -0.09618104906121516 1107 48-49 -0.15764139041248626 1107 50-51 -0.1242402491838206 1107 52-53 -0.20263707721623092 1107 54-55 7.100358373079985E-4 1107 56-57 -0.04099577513456154 1107 58-59 -0.2660004168354817 1107 60-61 -0.19689339097107705 1107 62-63 -0.15647684067658219 1107 64-65 -0.4109857175270051 1107 66-67 -0.23759864421545984 1107 68-69 -0.10307114390199601 1107 70-71 -0.2883586943157006 1107 72-73 -0.541112327835858 1107 74-75 -0.516710423760177 1107 76-77 -0.6508317375358814 1107 78-79 -0.438025088678323 1107 80-81 -0.31827739852758086 1107 82-83 -0.27071265033960046 1107 84-85 -0.3824664113878562 1107 86-87 -0.41009979215379744 1107 88-89 -0.4992508495659074 1107 90-91 -0.7812929290965975 1107 92-93 -0.9122662017435559 1107 94-95 -1.0658178290681697 1107 96-97 -1.0546662488924632 1107 98-99 -1.3186877679980746 1107 100-101 -1.629792581510479 1108 1 -0.008335373595734552 1108 2 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0.46495567071497845 2316 66-67 0.49072801799005816 2316 68-69 0.5335445196824757 2316 70-71 0.5681848080881977 2316 72-73 0.8058788669816721 2316 74-75 1.0545373978973593 2316 76-77 0.9882164305376975 2316 78-79 1.1541694269307001 2316 80-81 1.162986384399158 2316 82-83 1.3065188816420878 2316 84-85 1.5035218365980008 2316 86-87 1.81882806608753 2316 88-89 1.8345994888165293 2316 90-91 1.8370984609992895 2316 92-93 1.8086467687298615 2316 94-95 2.3495514253604632 2316 96-97 2.6660513549396505 2316 98-99 3.1404768961531566 2316 100-101 3.6094578391501813 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 1.0 3 5.0 4 18.0 5 40.0 6 73.0 7 196.0 8 548.0 9 1016.0 10 1672.0 11 2715.0 12 4285.0 13 6331.0 14 8501.0 15 11781.0 16 15664.0 17 21942.0 18 22841.0 19 18254.0 20 17379.0 21 18125.0 22 19323.0 23 20719.0 24 23922.0 25 27183.0 26 31242.0 27 37029.0 28 44391.0 29 52875.0 30 63918.0 31 77790.0 32 96319.0 33 124753.0 34 167218.0 35 239256.0 36 336777.0 37 404228.0 38 206353.0 39 10345.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.451190098663915 18.473522160853616 14.92226756035512 23.153020180127346 2 15.989356918003297 19.57591627941838 39.528931194723434 24.905795607854895 3 19.79367796224315 23.245260251693587 29.47567427293568 27.485387513127584 4 13.375709065974343 16.335390606301466 35.64053288319071 34.64836744453349 5 14.062041863972535 36.9442297694156 33.833284153235816 15.160444213376051 6 33.38585509614106 35.34811436879271 16.41489319846294 14.851137336603298 7 29.437037828075326 32.43278308293849 19.483772578158227 18.646406510827962 8 27.137864234099034 34.56863329192873 18.71329087955755 19.58021159441469 9 26.39159767459724 16.623950599242725 19.68718911414745 37.29726261201258 10-11 25.738350972446263 26.14213958786489 26.12733884520484 21.992170594484005 12-13 26.5523738151416 23.43480424587678 26.863777144161922 23.149044794819705 14-15 23.0948493415543 24.725952071822945 25.432945141703062 26.74625344491969 16-17 22.60050876759925 27.960226945385003 26.344531497735556 23.09473278928019 18-19 22.66131872743589 26.218719379792677 28.821378454989816 22.298583437781613 20-21 23.02667693351094 25.34332102436127 29.34631302259268 22.283689019535107 22-23 22.974523050751557 25.271940227481792 29.178305858283828 22.57523086348282 24-25 22.85049169424323 25.428543325066215 29.133592353973015 22.587372626717535 26-27 22.22108751200132 25.824633085854597 29.122673548007494 22.83160585413659 28-29 22.49356468536341 26.16373744993617 28.953259860710073 22.389438003990353 30-31 22.482190541185528 25.723608065908465 28.81748191184757 22.976719481058435 32-33 22.23947414272787 25.721442529090954 28.90849675039391 23.13058657778727 34-35 22.964364567063203 25.909411667440725 28.673931326968034 22.45229243852804 36-37 23.06684387093752 25.443106304998214 28.6637431865153 22.82630663754896 38-39 22.543008619756915 25.81538272741793 28.636675114837196 23.004933537987963 40-41 23.086213344943076 25.599622667756762 28.583760521426697 22.73040346587347 42-43 22.692271121755045 25.63512691296868 28.51882488113399 23.153777084142284 44-45 22.63912291527861 25.92034528829254 28.629678072062305 22.81085372436655 46-47 23.131730603496727 26.214553091674848 27.904861959397177 22.748854345431248 48-49 22.88784716017816 26.22043967279924 27.792486204458555 23.099226962564043 50-51 23.04934350660194 26.499836178180995 27.693264663223793 22.757555651993275 52-53 23.424936915972122 26.238880371510703 27.553281236020688 22.782901476496487 54-55 22.9714857709901 26.44793165334938 27.69387902794605 22.886703547714475 56-57 22.93030525691749 26.595084099483547 27.60321449309241 22.87139615050656 58-59 22.99072303541775 26.248494657130856 27.450824685444537 23.309957622006856 60-61 22.6839152024795 26.511274649059963 27.698237588845988 23.10657255961455 62-63 22.750520013492242 26.54516621692527 27.85473352822127 22.849580241361217 64-65 22.515818970959337 26.65818397065599 27.612651241496124 23.213345816888545 66-67 22.441298521281826 26.56853672412852 27.608485513677316 23.381679240912334 68-69 22.84203133360383 26.25215407984362 27.825674851311096 23.08013973524145 70-71 22.797492619426624 26.406033191265298 27.262142226061503 23.534331963246572 72-73 22.785240581445265 26.53460560493676 27.266271851213265 23.413881962404705 74-75 22.58469203885114 26.95192045760907 27.217770694684233 23.245616808855555 76-77 22.791577870359657 26.49949615074158 27.40188056875235 23.30704541014641 78-79 22.907473094526516 26.662573433401253 27.41219265026469 23.01776082180755 80-81 23.004151294105743 26.673212301546407 27.17322260717384 23.14941379717401 82-83 22.871709379603494 26.741737605001852 27.234557558446816 23.151995456947834 84-85 22.845007005069746 26.84085994756212 27.14856299444537 23.165570052922767 86-87 23.071668293258877 26.917016622163793 26.846080646801155 23.16523443777617 88-89 22.921382319671437 27.064691790424376 26.609974591399865 23.403951298504317 90-91 22.798586665148665 26.955429071003845 26.80791581835419 23.4380684454933 92-93 22.924799059999962 26.690140119224086 26.933104642468287 23.45195617830766 94-95 22.70787024783516 26.467956630603577 27.042725273283878 23.78144784827738 96-97 22.935310716135277 26.200189396645435 27.090419213992213 23.774080673227076 98-99 22.72826709163287 26.424002158558483 26.727268383582587 24.120462366226057 100-101 23.23931538853399 25.85981040267474 26.672116077127118 24.228758131664158 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1088.0 1 736.5 2 545.5 3 700.0 4 575.0 5 512.0 6 1047.0 7 2460.5 8 3899.5 9 4351.0 10 3425.5 11 2353.0 12 2204.5 13 2310.5 14 2436.5 15 2656.5 16 2816.5 17 2943.5 18 3185.0 19 3430.0 20 3792.5 21 4405.0 22 5259.5 23 6316.0 24 7710.5 25 9453.5 26 11713.0 27 14717.5 28 17135.0 29 19288.0 30 21855.0 31 25323.5 32 31151.5 33 33961.0 34 34098.0 35 35962.0 36 39338.5 37 42928.0 38 45718.0 39 48488.5 40 51785.0 41 56128.0 42 59054.5 43 62098.0 44 65637.5 45 69227.0 46 72088.0 47 76023.0 48 78888.5 49 78139.5 50 78301.0 51 78760.0 52 78901.5 53 80527.5 54 83022.0 55 79749.5 56 73996.0 57 69545.5 58 65687.5 59 59370.5 60 48267.5 61 39701.5 62 33459.0 63 25453.5 64 18353.0 65 13834.5 66 10196.5 67 7517.5 68 5677.0 69 4252.5 70 3148.0 71 2076.5 72 1382.5 73 1054.5 74 735.0 75 461.0 76 310.5 77 192.0 78 115.0 79 73.5 80 39.5 81 20.0 82 11.0 83 7.0 84 4.5 85 3.5 86 2.0 87 1.5 88 1.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.009601747611740924 2 0.014426040314225388 3 0.009835936577880946 4 4.6837793228004507E-5 5 8.430802781040812E-4 6 0.0018735117291201803 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 7.259857950340698E-4 14-15 0.0 16-17 0.003044456559820293 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0029507809733642838 26-27 0.017166051218063652 28-29 0.006018656429798579 30-31 0.0031381321462763015 32-33 0.0 34-35 0.008079519331830777 36-37 7.259857950340698E-4 38-39 0.002295051868172221 40-41 0.001850092832506178 42-43 0.0028571053869082745 44-45 2.3418896614002253E-5 46-47 1.8735117291201803E-4 48-49 3.9812124243803826E-4 50-51 0.00405146911422239 52-53 0.008290289401356798 54-55 0.0068617367079026595 56-57 0.017962293702939728 58-59 0.025643691792332467 60-61 0.003910955734538376 62-63 0.02192008723070611 64-65 0.022786586405424192 66-67 0.014402621417611386 68-69 0.03306748201897118 70-71 0.017329983494361666 72-73 0.00498822497878248 74-75 0.017212889011291654 76-77 0.021592222678110076 78-79 0.028617891662310754 80-81 0.012739879758017224 82-83 0.036533478717843515 84-85 0.023535991097072263 86-87 0.03320799539865519 88-89 0.024098044615808317 90-91 0.0301401199422209 92-93 0.026229164207682523 94-95 0.016065363077205547 96-97 0.016603997699327595 98-99 0.012646204171561214 100-101 0.011264489271335084 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 2135028.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 52.63319526204615 #Duplication Level Percentage of deduplicated Percentage of total 1 74.95541038999652 39.45142751003489 2 13.905455699790917 14.637771301096558 3 4.716916296788123 7.447991294507308 4 2.1174194429533064 4.457862039704575 5 1.1362054181092307 2.9901060814568963 6 0.7006662878428844 2.212698332494054 7 0.45046458569489567 1.6596573348261316 8 0.3270841780687226 1.3772388329133558 9 0.24210752043459927 1.1468603157699682 >10 1.3098704940854606 12.40084741440243 >50 0.0860424987010013 3.0845699317378013 >100 0.04567045420032472 4.501056231211046 >500 0.003833727111502934 1.391680986212348 >1k 0.0025855368891531125 2.091862123727064 >5k 1.783128889071112E-4 0.6718848285594712 >10k+ 8.91564444535556E-5 0.47648544134605875 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10154 0.47559095243715777 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8638 0.40458485790350296 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5680 0.2660386655350656 No Hit GTGTACAGGCCAGCCCTGGCTGCCTCAACACCTCAACCCCCTCCCAGGGA 2674 0.12524425909168405 No Hit AAGGTAAGGTGTGCACTTTTATTGGTCTCAAGTCAGTGTACAGGCCAGCC 2488 0.1165324295512752 No Hit CTCCTAAGAGGAGGATGGTCGCGTCCATGCCCTGAGTCCACCCCGGGGAA 2354 0.1102561652587226 No Hit CTCCCAGGGAGACCAAAGCCTTCATACATCAAGTTGGGGGGACAAAAAAA 2335 0.10936624718739052 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2287 0.1071180331124463 No Hit GACCTGGGCCATTCAGAAATTAAAAACAAAAATGAAGTATTAAGGCGGAA 2138 0.10013920192147364 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.6837793228004507E-5 7 0.0 0.0 0.0 0.0 4.6837793228004507E-5 8 0.0 0.0 0.0 0.0 4.6837793228004507E-5 9 0.0 0.0 0.0 0.0 4.6837793228004507E-5 10-11 0.0 0.0 0.0 2.3418896614002253E-5 4.6837793228004507E-5 12-13 0.0 0.0 0.0 4.6837793228004507E-5 1.8735117291201803E-4 14-15 0.0 0.0 0.0 4.6837793228004507E-5 1.8735117291201803E-4 16-17 0.0 0.0 0.0 4.6837793228004507E-5 1.8735117291201803E-4 18-19 0.0 0.0 0.0 4.6837793228004507E-5 2.3418896614002253E-4 20-21 0.0 0.0 0.0 1.1709448307001127E-4 2.8102675936802704E-4 22-23 0.0 0.0 0.0 3.512834492100338E-4 2.8102675936802704E-4 24-25 0.0 0.0 0.0 4.449590356660428E-4 2.8102675936802704E-4 26-27 0.0 0.0 0.0 0.0010772692442441035 3.278645525960315E-4 28-29 0.0 0.0 0.0 0.0032083888361183087 3.278645525960315E-4 30-31 0.0 0.0 0.0 0.011170813684879074 3.278645525960315E-4 32-33 0.0 0.0 0.0 0.027985581453732693 3.278645525960315E-4 34-35 0.0 0.0 0.0 0.05163866703387497 3.278645525960315E-4 36-37 0.0 0.0 0.0 0.09098241334539875 3.278645525960315E-4 38-39 0.0 0.0 0.0 0.15093478867724452 3.278645525960315E-4 40-41 0.0 0.0 0.0 0.2150323087097687 3.278645525960315E-4 42-43 0.0 0.0 0.0 0.27962162557118686 3.7470234582403605E-4 44-45 0.0 0.0 0.0 0.3586135638502165 4.215401390520406E-4 46-47 0.0 0.0 0.0 0.45474813445069573 4.6837793228004507E-4 48-49 0.0 0.0 0.0 0.5495946657374049 4.6837793228004507E-4 50-51 0.0 0.0 0.0 0.6520523384236647 4.6837793228004507E-4 52-53 0.0 0.0 0.0 0.7468754508137598 4.6837793228004507E-4 54-55 0.0 0.0 0.0 0.8463823425266555 4.6837793228004507E-4 56-57 0.0 0.0 0.0 0.967177011261679 4.917968288940473E-4 58-59 0.0 0.0 0.0 1.0953720513267273 5.152157255080495E-4 60-61 0.0 0.0 0.0 1.2297262612012583 5.152157255080495E-4 62-63 0.0 0.0 0.0 1.3593030161665327 5.152157255080495E-4 64-65 0.0 0.0 0.0 1.4948281708717637 5.152157255080495E-4 66-67 0.0 0.0 0.0 1.6592054062054455 5.152157255080495E-4 68-69 0.0 0.0 0.0 1.8364864535734426 5.152157255080495E-4 70-71 0.0 0.0 0.0 2.0222685604123223 5.62053518736054E-4 72-73 0.0 0.0 0.0 2.215006079545561 6.55729105192063E-4 74-75 0.0 0.0 0.0 2.400530578521687 7.025668984200676E-4 76-77 0.0 0.0 0.0 2.5990759840151982 7.259857950340698E-4 78-79 0.0 0.0 0.0 2.8240144859926897 7.494046916480721E-4 80-81 0.0 0.0 0.0 3.0623720157300047 7.494046916480721E-4 82-83 0.0 0.0 0.0 3.306373499551294 7.728235882620743E-4 84-85 0.0 0.0 0.0 3.5509370368913196 8.430802781040811E-4 86-87 0.0 0.0 0.0 3.806694806812838 8.430802781040811E-4 88-89 0.0 0.0 0.0 4.079431276779509 8.430802781040811E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 5005 0.0 52.775013 1 GTATCAA 8635 0.0 39.44705 1 TATCAAC 11140 0.0 30.490051 2 ATCAACG 11205 0.0 30.224136 3 TCAACGC 11335 0.0 29.9571 4 CAACGCA 11500 0.0 29.568577 5 AACGCAG 11815 0.0 28.94103 6 ACGCAGA 13275 0.0 25.47186 7 CGCAGAG 13360 0.0 25.274256 8 GTACATG 7755 0.0 24.994015 1 GTAAGGT 1075 0.0 24.73972 4 TAAGGTG 1215 0.0 24.234308 5 TACATGG 7830 0.0 24.086077 2 ACATGGG 7835 0.0 23.157978 3 GAGTACT 9095 0.0 23.029346 12-13 ATAGCGT 165 2.435323E-6 23.026146 6 TGGTATC 2360 0.0 22.745932 2 GCAGAGT 14755 0.0 22.530666 9 GTACTTT 9345 0.0 22.081873 14-15 GTGTAAG 895 0.0 21.762985 1 >>END_MODULE