FastQCFastQC Report
Thu 26 May 2016
SRR938119_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938119_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427504
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT95670.6701907665407593No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82340.5768109931740997No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT48610.34052443986146447No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20110.14087526199576325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15540.10886134119414026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35350.060.7262231
GTATCAA64300.043.697591
GTGGTAT13800.033.5309451
TATCAAC83250.033.2222672
ATCAACG83600.033.1400223
TCAACGC84650.032.8973734
TGGTATC13600.032.845772
CAACGCA85050.032.7985275
AACGCAG88150.031.6967776
ACGCAGA99350.027.8765477
CGCAGAG100500.027.3212228
GTACATG63800.026.7679751
GTGTACG1457.6003926E-726.3193511
TACATGG63500.026.1929762
TACCTGG13150.025.6580182
GTACCTG14500.025.0033841
GCAGAGT108650.024.8783079
ACATGGG65750.024.6461523
GAGTACT72300.023.78529712-13
CAGAGTA105350.022.81717510-11
Produced by FastQC (version 0.11.5)