FastQCFastQC Report
Thu 26 May 2016
SRR938119_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938119_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427504
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91190.6388073168271332No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84390.591171723511808No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51790.3628010849706901No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22320.15635682982324395No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16560.11600668019143906No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34600.063.086351
GTATCAA66200.044.5381851
TGGTATC13000.036.580992
GTGGTAT13350.035.978161
ATCAACG85150.034.286383
TATCAAC86150.034.169152
TCAACGC86850.033.7783164
CAACGCA87800.033.4116675
AACGCAG90150.032.559196
ACGCAGA102850.028.3540387
CGCAGAG102850.028.21558
GCAGAGT110450.025.9299969
GAGTACT73400.023.52118512-13
GTACATG62800.023.247571
CAGAGTA107400.023.15065410-11
TACATGG63400.022.8775442
GTACTTT77400.022.15221214-15
ACATGGG63800.021.7620643
AGAGTAC106650.021.64343810-11
AGTACTT76000.021.5291312-13
Produced by FastQC (version 0.11.5)