##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938119_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1427504 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.46064879678095 33.0 31.0 34.0 31.0 34.0 2 32.68916514419574 34.0 31.0 34.0 31.0 34.0 3 32.74539686053419 34.0 31.0 34.0 31.0 34.0 4 36.22022214999047 37.0 35.0 37.0 35.0 37.0 5 36.14810676537509 37.0 35.0 37.0 35.0 37.0 6 36.20047509499098 37.0 37.0 37.0 35.0 37.0 7 36.17345100258913 37.0 37.0 37.0 35.0 37.0 8 36.167886044452416 37.0 37.0 37.0 35.0 37.0 9 37.848291143142156 39.0 38.0 39.0 35.0 39.0 10-11 37.82291538237371 39.0 38.0 39.0 35.0 39.0 12-13 37.73251808751499 39.0 38.0 39.0 35.0 39.0 14-15 38.946260395767716 40.0 38.0 41.0 35.5 41.0 16-17 38.84677310886694 40.0 38.0 41.0 35.0 41.0 18-19 39.11567568287024 40.0 38.5 41.0 36.0 41.0 20-21 39.0972519166321 40.0 39.0 41.0 36.0 41.0 22-23 38.98876920835248 40.0 38.5 41.0 35.0 41.0 24-25 38.87603677467804 40.0 38.0 41.0 35.0 41.0 26-27 38.70617630493505 40.0 38.0 41.0 35.0 41.0 28-29 38.575937440455505 40.0 38.0 41.0 34.0 41.0 30-31 38.316944470908666 40.0 38.0 41.0 34.0 41.0 32-33 38.0650807283202 40.0 37.0 41.0 33.5 41.0 34-35 37.95948908024076 40.0 37.0 41.0 33.0 41.0 36-37 37.896500465147554 40.0 37.0 41.0 33.0 41.0 38-39 37.707407825126936 40.0 37.0 41.0 33.0 41.0 40-41 37.47831459666663 40.0 37.0 41.0 33.0 41.0 42-43 37.20716614454321 40.0 36.0 41.0 32.0 41.0 44-45 36.8960104490075 40.0 36.0 41.0 31.0 41.0 46-47 36.47095349645255 39.0 35.5 41.0 30.5 41.0 48-49 36.019166321075105 39.0 35.0 40.5 29.5 41.0 50-51 35.62238739786368 39.0 35.0 40.0 28.0 41.0 52-53 35.080393470000786 38.0 34.5 40.0 26.5 41.0 54-55 34.69212695726247 38.0 34.0 40.0 26.0 41.0 56-57 34.30185694751118 38.0 34.0 40.0 24.5 41.0 58-59 33.79639496631883 37.0 33.0 40.0 23.0 41.0 60-61 33.31436899651419 37.0 32.5 39.5 22.0 41.0 62-63 32.749543258722916 36.0 32.0 39.0 19.5 40.0 64-65 32.17569127652182 35.5 31.0 39.0 17.5 40.0 66-67 31.612491803875855 35.0 31.0 38.0 12.0 40.0 68-69 30.999462348266626 35.0 30.0 38.0 7.5 40.0 70-71 30.373416116522264 34.5 29.0 37.0 6.0 39.0 72-73 29.706719560855873 34.0 28.5 36.5 2.0 39.0 74-75 29.045287088512538 34.0 27.5 36.0 2.0 39.0 76-77 27.74964798697587 31.5 26.0 34.5 2.0 36.5 78-79 28.416145593987828 33.5 27.0 35.0 2.0 37.0 80-81 28.13780241596521 33.5 27.0 35.0 2.0 37.0 82-83 27.58274302558872 33.0 26.0 35.0 2.0 36.0 84-85 26.922839445633777 33.0 25.5 35.0 2.0 36.0 86-87 26.19758228348222 32.0 23.5 35.0 2.0 35.0 88-89 25.39429696869501 32.0 20.5 34.0 2.0 35.0 90-91 24.736415099362244 31.0 18.0 34.0 2.0 35.0 92-93 23.79939285634226 31.0 6.0 34.0 2.0 35.0 94-95 22.889669310909113 30.5 2.0 34.0 2.0 35.0 96-97 21.83630763906791 30.0 2.0 34.0 2.0 35.0 98-99 20.44055568320649 29.0 2.0 34.0 2.0 35.0 100-101 16.649732329996972 20.5 2.0 31.0 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.09722570739994296 1101 2 0.1184733486006948 1101 3 0.16074178043768228 1101 4 0.0545616339805477 1101 5 0.03524815657368663 1101 6 0.03345719893248855 1101 7 0.11752758858861512 1101 8 0.08768698429021526 1101 9 -0.03116640639964885 1101 10-11 0.12939190838548598 1101 12-13 0.244824003140522 1101 14-15 0.33001900553849595 1101 16-17 0.4539710226335174 1101 18-19 0.41660732509579645 1101 20-21 0.2817938993172291 1101 22-23 0.27088558711736965 1101 24-25 0.2732035473964274 1101 26-27 0.4311762643230992 1101 28-29 0.3533295948262989 1101 30-31 0.3850924841986867 1101 32-33 0.44533467421856443 1101 34-35 0.3285056314833028 1101 36-37 0.4305507007517235 1101 38-39 0.4454469796447569 1101 40-41 0.5350433782729809 1101 42-43 0.552085450213184 1101 44-45 0.722142087308761 1101 46-47 0.5608549124660485 1101 48-49 0.746129273875539 1101 50-51 0.7718961173422016 1101 52-53 0.8744404611687244 1101 54-55 1.1238571053380824 1101 56-57 1.0495399289999767 1101 58-59 1.1507797354278466 1101 60-61 1.1121664250722034 1101 62-63 1.2001023119880827 1101 64-65 1.3685134764931561 1101 66-67 1.4260892625141537 1101 68-69 1.5705234395402385 1101 70-71 1.5996062321984965 1101 72-73 1.8152430522049592 1101 74-75 1.7810747420326685 1101 76-77 1.8960497636114013 1101 78-79 1.8908016416447886 1101 80-81 2.0775053837125697 1101 82-83 2.2493727428935273 1101 84-85 2.376695729627027 1101 86-87 2.6427947237540543 1101 88-89 3.1108619177739847 1101 90-91 3.0866944818173643 1101 92-93 3.096871147704597 1101 94-95 3.4072383623777007 1101 96-97 3.778229586474783 1101 98-99 3.996701784274869 1101 100-101 4.144957452301636 1102 1 0.0915020769829411 1102 2 0.1364619013398638 1102 3 0.12312935198306008 1102 4 0.07009720225527616 1102 5 -0.09230703557676634 1102 6 0.08905818012627265 1102 7 0.08563879055100188 1102 8 0.14328796548399936 1102 9 0.017075621400842067 1102 10-11 0.1363420310347081 1102 12-13 0.29183953870879975 1102 14-15 0.3655872802727558 1102 16-17 0.2875769097962291 1102 18-19 0.31644379279816803 1102 20-21 0.25808171616105113 1102 22-23 0.2389967890797564 1102 24-25 0.30141286873739404 1102 26-27 0.36167503783087085 1102 28-29 0.24826009359981072 1102 30-31 0.43742281943990236 1102 32-33 0.24255462515887416 1102 34-35 0.312152401720418 1102 36-37 0.4289153777754393 1102 38-39 0.5488811578949608 1102 40-41 0.5853295597938271 1102 42-43 0.6473430135819456 1102 44-45 0.3909891846104756 1102 46-47 0.654885983602604 1102 48-49 0.7555323809891945 1102 50-51 0.7608576872522619 1102 52-53 0.8245631103919422 1102 54-55 1.000390220628347 1102 56-57 1.1084115561463435 1102 58-59 1.1941157942994778 1102 60-61 0.9731639720877396 1102 62-63 1.0917621648090048 1102 64-65 1.1023646621023104 1102 66-67 1.0605945773138217 1102 68-69 1.1980786316906844 1102 70-71 1.4953543924601505 1102 72-73 1.5985627578468247 1102 74-75 1.8068310789092017 1102 76-77 1.8592549966449212 1102 78-79 1.936590684980846 1102 80-81 2.0325340018646543 1102 82-83 1.9566499301380098 1102 84-85 2.2221577083678277 1102 86-87 2.357839695135901 1102 88-89 2.515195523661145 1102 90-91 2.5225080549980667 1102 92-93 2.784524459233623 1102 94-95 3.055235091731749 1102 96-97 3.4593416060986577 1102 98-99 3.6283452838660395 1102 100-101 3.4528070025878215 1103 1 0.2478455779379587 1103 2 0.30569444907304444 1103 3 0.28794561844588884 1103 4 0.15309276584095954 1103 5 0.04729810991047856 1103 6 0.10668888419191092 1103 7 0.16298151411062634 1103 8 0.18965242429145945 1103 9 0.3050522306969796 1103 10-11 0.3126847916131581 1103 12-13 0.3734511176787265 1103 14-15 0.43387630250158793 1103 16-17 0.4934090638309243 1103 18-19 0.3566848827883433 1103 20-21 0.27324644669970866 1103 22-23 0.3511833224093692 1103 24-25 0.4355366298243766 1103 26-27 0.508031248583606 1103 28-29 0.5553471859137318 1103 30-31 0.49981448253998906 1103 32-33 0.7161959205061308 1103 34-35 0.7431717119102714 1103 36-37 0.7247305615998982 1103 38-39 0.7767167839060818 1103 40-41 1.0053507059876736 1103 42-43 1.1001797274270473 1103 44-45 1.0612037054731829 1103 46-47 0.9867052252102013 1103 48-49 1.1784799629777822 1103 50-51 1.210528317098401 1103 52-53 1.3814730643652027 1103 54-55 1.3628815098382674 1103 56-57 1.4656650058789893 1103 58-59 1.6671339393704159 1103 60-61 1.5987821702839469 1103 62-63 1.7590206997596525 1103 64-65 1.6517318674470474 1103 66-67 1.7722662891488739 1103 68-69 1.9293729203349343 1103 70-71 1.9602917169860348 1103 72-73 2.1152114887829363 1103 74-75 2.272539953751142 1103 76-77 2.1683560431087514 1103 78-79 2.338173207417828 1103 80-81 2.518162903048413 1103 82-83 2.698612785315806 1103 84-85 2.9585342924651847 1103 86-87 3.047881370543127 1103 88-89 3.3615271201403303 1103 90-91 3.5762675118474263 1103 92-93 3.7163068768488685 1103 94-95 3.721007028472414 1103 96-97 3.794380836314854 1103 98-99 4.250126512982064 1103 100-101 4.037816520323407 1104 1 0.136908544760324 1104 2 0.20704084068038497 1104 3 0.22835388382809896 1104 4 0.1994458736178757 1104 5 0.0955506913242985 1104 6 0.05120882987959874 1104 7 0.049150334080849234 1104 8 0.14294164708611845 1104 9 0.1782168217879132 1104 10-11 0.16034645940931114 1104 12-13 0.2364467236278216 1104 14-15 0.30005996362772436 1104 16-17 0.2863160357736092 1104 18-19 0.2553645421461681 1104 20-21 0.3322588661481731 1104 22-23 0.3256354627178055 1104 24-25 0.4192614563334516 1104 26-27 0.36093832983103624 1104 28-29 0.3296486678432231 1104 30-31 0.35270577532368463 1104 32-33 0.4154059507956873 1104 34-35 0.4635823091271334 1104 36-37 0.431594656933072 1104 38-39 0.5306881897837599 1104 40-41 0.6500395275124546 1104 42-43 0.6717403320523445 1104 44-45 0.6778670929973813 1104 46-47 0.6618651744927959 1104 48-49 0.7199869232612954 1104 50-51 0.7853862816496573 1104 52-53 0.9774198621004118 1104 54-55 1.025375467714646 1104 56-57 0.9526389160499207 1104 58-59 1.2531762087313254 1104 60-61 1.1322391394332882 1104 62-63 1.1122912192972692 1104 64-65 1.264115670426456 1104 66-67 1.3542797676389142 1104 68-69 1.48330965231726 1104 70-71 1.6032325734008879 1104 72-73 1.74731963733052 1104 74-75 1.5758664008852925 1104 76-77 1.691249315522903 1104 78-79 1.9060277738058922 1104 80-81 1.9164126303937898 1104 82-83 2.1063198024377847 1104 84-85 2.2206783536952805 1104 86-87 2.2259867791850034 1104 88-89 2.439107683812775 1104 90-91 2.5789463890690456 1104 92-93 3.0246959556041553 1104 94-95 3.3345543197792864 1104 96-97 3.8187372742431194 1104 98-99 4.087229038407564 1104 100-101 3.753632978218004 1105 1 0.16525928944626855 1105 2 0.1721634042882627 1105 3 0.15352611597034382 1105 4 0.11525394509677511 1105 5 0.09983853621616845 1105 6 0.06488991136746591 1105 7 0.1217683310569484 1105 8 0.07087096178348418 1105 9 0.12815319680587578 1105 10-11 0.21041264991482223 1105 12-13 0.21271905223375143 1105 14-15 0.3157934638911115 1105 16-17 0.23533224304054556 1105 18-19 0.22665376900001633 1105 20-21 0.2716475190089014 1105 22-23 0.2582331749550022 1105 24-25 0.2895579966395374 1105 26-27 0.31135958878471826 1105 28-29 0.29603688969601194 1105 30-31 0.4613069493072288 1105 32-33 0.40872618285584394 1105 34-35 0.3793305183917468 1105 36-37 0.2029209983597795 1105 38-39 0.34593800335572666 1105 40-41 0.43967087889338785 1105 42-43 0.4032326457440334 1105 44-45 0.5665507400087151 1105 46-47 0.5358150202193244 1105 48-49 0.5544995422160639 1105 50-51 0.4688485752960503 1105 52-53 0.5723810029715963 1105 54-55 0.5366697572014729 1105 56-57 0.8159119082712536 1105 58-59 0.9146502558959071 1105 60-61 0.8360169582594423 1105 62-63 0.9220559436916389 1105 64-65 1.0222646281397871 1105 66-67 1.191226213191662 1105 68-69 1.28975031346976 1105 70-71 1.4014986216053629 1105 72-73 1.5754740339536077 1105 74-75 1.424894751629978 1105 76-77 1.58205037457099 1105 78-79 1.637330238760839 1105 80-81 1.8830394459292314 1105 82-83 1.9223572317148587 1105 84-85 2.0078810095251747 1105 86-87 2.0017822624954142 1105 88-89 2.1891684012015524 1105 90-91 2.494784391655095 1105 92-93 2.4441992360534996 1105 94-95 2.79905004048614 1105 96-97 3.1922544016778005 1105 98-99 3.1571600771367017 1105 100-101 2.8713417605179465 1106 1 0.06766769386809557 1106 2 0.07339833639567672 1106 3 0.07973339117091172 1106 4 0.12104919384504598 1106 5 0.01185105762466776 1106 6 -0.018349593348546023 1106 7 0.0680803337447955 1106 8 0.07745453481001618 1106 9 0.11084611909610942 1106 10-11 0.15978944761376823 1106 12-13 0.10910357747733457 1106 14-15 5.1562243172043054E-5 1106 16-17 0.07282845301996588 1106 18-19 0.020131655766491008 1106 20-21 -0.03855808448528819 1106 22-23 -0.10242600181155836 1106 24-25 -0.14668689195435292 1106 26-27 -0.11510008129911142 1106 28-29 0.026025990826049394 1106 30-31 0.14784725463947268 1106 32-33 0.13001175783269048 1106 34-35 0.1694330333264773 1106 36-37 0.19770347166573998 1106 38-39 0.1363658454614125 1106 40-41 0.20611792923305217 1106 42-43 0.21784040934508653 1106 44-45 0.4018278417287746 1106 46-47 0.6500015793723222 1106 48-49 0.5958808305772152 1106 50-51 0.7442564634556206 1106 52-53 0.6710272485977029 1106 54-55 0.6735784115597951 1106 56-57 0.6988482113417831 1106 58-59 0.7700058622582304 1106 60-61 0.7084665817157756 1106 62-63 0.7613418628433308 1106 64-65 0.8548833889464689 1106 66-67 1.026045234587869 1106 68-69 1.1194935754277893 1106 70-71 0.9760944210333733 1106 72-73 1.3055311505879068 1106 74-75 1.4171180391552767 1106 76-77 1.3210675095612814 1106 78-79 1.516507660891282 1106 80-81 1.4867212972620898 1106 82-83 1.6563576086682552 1106 84-85 1.8629784847568764 1106 86-87 2.1594528543941927 1106 88-89 2.0151847413118524 1106 90-91 2.189012087896213 1106 92-93 2.1044511392584226 1106 94-95 2.6015797255661575 1106 96-97 3.0134654054391383 1106 98-99 2.9014996302010907 1106 100-101 2.8716883937504853 1107 1 0.23961721408851133 1107 2 0.2040356145833897 1107 3 0.2439087930984769 1107 4 0.12113153225591589 1107 5 0.05808671437905133 1107 6 0.09517237939532919 1107 7 0.12662083263111157 1107 8 0.17766038083718172 1107 9 0.20793418952712273 1107 10-11 0.2646954445946932 1107 12-13 0.24808316177391987 1107 14-15 0.36211545896124875 1107 16-17 0.37471352759956744 1107 18-19 0.33799724358885186 1107 20-21 0.4189896031776712 1107 22-23 0.38443125716386106 1107 24-25 0.3841097849518391 1107 26-27 0.40991205833838507 1107 28-29 0.40500515287438077 1107 30-31 0.5088871993249526 1107 32-33 0.5280248158601566 1107 34-35 0.460623351173858 1107 36-37 0.503616530748829 1107 38-39 0.47485245491344585 1107 40-41 0.35993716475258 1107 42-43 0.5037418038199633 1107 44-45 0.4893023748805376 1107 46-47 0.5086217776178827 1107 48-49 0.5484301544521841 1107 50-51 0.4929628003463904 1107 52-53 0.4505975582112427 1107 54-55 0.5071270915061703 1107 56-57 0.7305606391637269 1107 58-59 0.9527097817777062 1107 60-61 0.9721392443077477 1107 62-63 0.9767475906409899 1107 64-65 1.0076799919592077 1107 66-67 1.3510729446299834 1107 68-69 1.377651939638028 1107 70-71 1.4781885085021003 1107 72-73 1.6315743676974037 1107 74-75 1.53062496930486 1107 76-77 1.4450620308900959 1107 78-79 1.5567925201242652 1107 80-81 1.7645749037900451 1107 82-83 1.9906747804494032 1107 84-85 1.8147007298508804 1107 86-87 1.8941891075742525 1107 88-89 2.0105864577510317 1107 90-91 2.4936940293937155 1107 92-93 2.737620851390666 1107 94-95 2.9745661291721603 1107 96-97 3.098918425042015 1107 98-99 3.5081028012855313 1107 100-101 3.076995526579232 1108 1 0.24738908771588797 1108 2 0.19114552105735783 1108 3 0.23484866004082505 1108 4 0.1120210472704315 1108 5 0.06265106307151314 1108 6 0.11239648832690108 1108 7 0.06733411589820548 1108 8 0.11399197506492698 1108 9 0.2285715163754105 1108 10-11 0.08722793230132453 1108 12-13 0.1411037953511567 1108 14-15 0.22837243591720124 1108 16-17 0.2733350096603573 1108 18-19 0.226356038570799 1108 20-21 0.2727713543534449 1108 22-23 0.2180473290706999 1108 24-25 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54-55 0.24044131056555074 2316 56-57 -0.012437328515176205 2316 58-59 0.15526227251463354 2316 60-61 0.3691459816808944 2316 62-63 0.5916750217479105 2316 64-65 0.6964759233961431 2316 66-67 0.839075061903408 2316 68-69 1.003790627581985 2316 70-71 1.1308127186088157 2316 72-73 1.1742866014862123 2316 74-75 1.5341815312187705 2316 76-77 1.5699991334929067 2316 78-79 1.4896107482800822 2316 80-81 1.5409991622542414 2316 82-83 1.9183452242272985 2316 84-85 2.0213685074326158 2316 86-87 2.0558901472600617 2316 88-89 2.2759906021177123 2316 90-91 2.610362166389148 2316 92-93 2.769835754643033 2316 94-95 3.4609247456254657 2316 96-97 3.5100247984271036 2316 98-99 3.923019776721233 2316 100-101 4.0689193374100014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5.0 3 4.0 4 9.0 5 25.0 6 52.0 7 153.0 8 408.0 9 735.0 10 1316.0 11 2151.0 12 3287.0 13 5026.0 14 6551.0 15 9189.0 16 12463.0 17 17187.0 18 18190.0 19 14184.0 20 13189.0 21 14092.0 22 15240.0 23 16669.0 24 18653.0 25 20889.0 26 23878.0 27 28066.0 28 33255.0 29 39585.0 30 46995.0 31 56242.0 32 68441.0 33 85095.0 34 111231.0 35 152712.0 36 204034.0 37 251545.0 38 131320.0 39 5438.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.7311559197032 18.79546397067889 14.760729216870846 22.71265089274706 2 15.994322122842245 19.942282388912165 40.016632873115135 24.046762615130447 3 19.43659051232546 24.099164104992674 30.06826148970333 26.395983892978535 4 13.105935412190547 16.82177651152413 35.94270474674654 34.129583329538775 5 13.547436471078333 37.29379487560717 33.99935198185549 15.159416671459008 6 32.38400733027718 35.63548683576368 16.899567356728948 15.080938477230186 7 28.693649895201695 33.120187404028286 19.905933713670855 18.28022898709916 8 26.77903553132362 35.05959015090007 18.97091490653947 19.19045941123684 9 26.61785886414329 16.47448973873278 19.95735213351416 36.95029926360977 10-11 25.490226297089187 26.565844999383536 26.290994631188426 21.65293407233885 12-13 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25.958313674763723 29.657802534440584 22.022957006139077 38-39 21.925243459494524 26.157012764875276 29.788422043395883 22.129321732234313 40-41 22.1794077331608 26.050927367671413 29.79658408102027 21.97308081814752 42-43 22.093590226801755 26.00647044235239 29.60101274052237 22.298926590323482 44-45 22.08169185669966 26.36517088695607 29.514038850411172 22.0390984059331 46-47 22.3071178784788 26.625774778914806 28.935365505105416 22.131741837500982 48-49 22.28942966184333 26.608086562279336 28.77844825653729 22.32403551934005 50-51 22.328483843127586 26.84864630852173 28.606329649514116 22.216540198836572 52-53 22.575208195563725 26.81946950761609 28.43358057140295 22.17174172541723 54-55 22.31986740492496 26.802762023784172 28.47046313005077 22.406907441240094 56-57 22.247363229805313 27.12675411067149 28.34198713278562 22.28389552673758 58-59 22.346697452336386 26.742937322767574 28.375857440679674 22.534507784216366 60-61 22.16309026104305 26.781466111478498 28.715961566482473 22.339482060995977 62-63 22.125297021934788 26.922901792219143 28.657818121700533 22.29398306414553 64-65 22.01013096986068 26.84917170109506 28.537433170064674 22.603264158979588 66-67 21.964620026935155 27.051686424367034 28.355291847124608 22.628401701573203 68-69 22.10779095821385 26.774849220084562 28.48406771444654 22.633292107255055 70-71 22.046523162106727 26.89971446664948 28.277468924780596 22.776293446463196 72-73 22.17850177652742 26.93249896322532 28.126366020690657 22.762633239556596 74-75 22.083720956298546 27.23778707450207 27.887697687712258 22.790794281487127 76-77 22.058852374494222 26.96073005749896 28.299255203488045 22.681162364518766 78-79 22.131846916015647 27.03446014862305 28.136348479583944 22.69734445577736 80-81 22.06385700335271 27.2706448047008 28.02731624894396 22.63818194300253 82-83 22.102706939997017 27.24758122536304 27.99529800438395 22.654413830255997 84-85 22.107079057059792 27.140320151318786 27.8975445724894 22.85505621913202 86-87 22.232889936278603 27.036093379587854 27.938427869284272 22.79258881484927 88-89 22.142459845996928 27.185948340600095 27.69617458164741 22.975417231755568 90-91 22.097661512293506 27.166591591644185 27.883563894214973 22.852183001847337 92-93 22.144596442461808 26.939469171364845 27.822198746903688 23.093735639269664 94-95 22.00943815549314 26.8667642496148 27.83261126855381 23.291186326338252 96-97 22.12557320410059 26.72661754589486 27.730188819350165 23.417620430654388 98-99 21.940919254867236 26.79890914491308 27.507208386106097 23.752963214113585 100-101 22.387152680482856 26.223254015400304 27.660623017518688 23.728970286598148 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1471.0 1 994.5 2 752.5 3 984.5 4 748.5 5 621.5 6 1198.0 7 2653.5 8 4225.5 9 4816.0 10 3809.5 11 2492.5 12 2228.0 13 2335.0 14 2399.0 15 2449.5 16 2554.0 17 2621.5 18 2719.0 19 2948.0 20 3236.5 21 3647.0 22 4288.5 23 5059.0 24 5993.0 25 7294.0 26 8879.5 27 10557.0 28 11977.5 29 13484.0 30 15608.5 31 17968.0 32 20709.5 33 23046.0 34 24901.0 35 26829.5 36 29656.5 37 32350.0 38 34231.0 39 35851.5 40 37082.5 41 39539.5 42 41848.5 43 44190.0 44 47142.0 45 49921.5 46 51237.5 47 51786.0 48 52873.5 49 52864.0 50 52404.0 51 51359.5 52 49982.0 53 49941.5 54 50188.5 55 47523.0 56 43181.0 57 39638.5 58 36619.5 59 32396.5 60 26889.5 61 22390.0 62 18572.0 63 14395.5 64 10921.0 65 8436.0 66 6335.5 67 5052.0 68 3791.0 69 2555.5 70 1887.0 71 1333.0 72 939.0 73 736.0 74 567.5 75 436.0 76 283.0 77 164.0 78 106.5 79 64.5 80 37.0 81 18.0 82 6.5 83 5.0 84 3.0 85 1.5 86 3.5 87 2.5 88 0.0 89 0.0 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.014710992053262198 3 0.0036427218417601635 4 0.0014710992053262198 5 0.11334469115322969 6 0.0 7 0.0 8 2.101570293323171E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 1.0507851466615855E-4 24-25 5.954449164415651E-4 26-27 0.006269684708414127 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.011523610441722055 36-37 6.654972595523376E-4 38-39 0.007740783913740347 40-41 0.01103324403994665 42-43 0.006304710879969513 44-45 0.003817852699537094 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.0507851466615855E-4 68-69 6.654972595523376E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.4010468622154476E-4 82-83 0.0017513085777693092 84-85 0.003782826527981708 86-87 0.0028020937244308945 88-89 0.0 90-91 0.0030122507537632117 92-93 0.0 94-95 3.502617155538619E-5 96-97 1.4010468622154476E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1427504.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.86276915084659 #Duplication Level Percentage of deduplicated Percentage of total 1 81.04110704507389 49.32386189813379 2 11.285283368255573 13.737071928880548 3 3.185370411217408 5.816113919935871 4 1.3674216546281612 3.3290027399000977 5 0.757555356381405 2.305345838721439 6 0.49108117271941054 1.7933136029729104 7 0.3262214900572775 1.3898320268980893 8 0.22834051557924104 1.1117948869987708 9 0.19455224077043848 1.0656889306012007 >10 1.0220158498093599 11.342073142533202 >50 0.07053615368609394 2.9171270094279516 >100 0.028096645913852702 3.1699196836673194 >500 0.0017272113628210638 0.7441998125557239 >1k 3.4544227256421274E-4 0.3612021691225351 >5k 3.4544227256421274E-4 1.5934524096505784 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9119 0.6388073168271332 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8439 0.591171723511808 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5179 0.3628010849706901 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2232 0.15635682982324395 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1656 0.11600668019143906 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 7.005234311077236E-5 0.0 0.0 0.0 0.0 12-13 1.4010468622154473E-4 0.0 0.0 0.0 0.0 14-15 1.4010468622154473E-4 0.0 0.0 0.0 0.0 16-17 1.4010468622154473E-4 0.0 0.0 0.0 0.0 18-19 1.7513085777693093E-4 0.0 0.0 0.0 0.0 20-21 2.101570293323171E-4 0.0 0.0 0.0 0.0 22-23 2.8020937244308946E-4 0.0 0.0 3.502617155538618E-5 0.0 24-25 2.8020937244308946E-4 0.0 0.0 2.802093724430895E-4 0.0 26-27 2.8020937244308946E-4 0.0 0.0 0.0012259160044385165 0.0 28-29 2.8020937244308946E-4 0.0 0.0 0.003852878871092481 0.0 30-31 2.8020937244308946E-4 0.0 0.0 0.011173348726168193 0.0 32-33 3.5026171555386186E-4 0.0 0.0 0.02819606810208588 0.0 34-35 4.553402302200204E-4 0.0 0.0 0.05366009482285163 0.0 36-37 4.903664017754066E-4 0.0 0.0 0.09814333269819209 0.0 38-39 4.903664017754066E-4 0.0 0.0 0.15887871417523175 0.0 40-41 4.903664017754066E-4 0.0 0.0 0.22098011634293144 0.0 42-43 4.903664017754066E-4 0.0 0.0 0.28567345520572973 0.0 44-45 5.604187448861789E-4 0.0 0.0 0.36213558771113774 0.0 46-47 5.604187448861789E-4 0.0 0.0 0.4553402302200204 0.0 48-49 5.604187448861789E-4 0.0 0.0 0.553623667604434 0.0 50-51 5.604187448861789E-4 0.0 0.0 0.6578265279817079 0.0 52-53 5.954449164415651E-4 0.0 0.0 0.7553043634203478 0.0 54-55 6.304710879969514E-4 0.0 0.0 0.8584914648225154 0.0 56-57 6.304710879969514E-4 0.0 0.0 0.988508613636109 0.0 58-59 6.304710879969514E-4 0.0 0.0 1.125215761216781 0.0 60-61 6.304710879969514E-4 0.0 0.0 1.2709946872302984 0.0 62-63 6.304710879969514E-4 0.0 0.0 1.4117648707113957 0.0 64-65 6.304710879969514E-4 0.0 0.0 1.5561427498626974 0.0 66-67 6.304710879969514E-4 0.0 0.0 1.7214662796041202 0.0 68-69 6.304710879969514E-4 0.0 0.0 1.9056689158138962 0.0 70-71 6.654972595523376E-4 0.0 0.0 2.1015002409800605 0.0 72-73 7.355496026631099E-4 0.0 0.0 2.2932335040742444 0.0 74-75 8.756542888846546E-4 0.0 0.0 2.486648023403087 0.0 76-77 9.106804604400408E-4 0.0 0.0 2.701043219493606 0.0 78-79 9.807328035508131E-4 0.0 0.0 2.9328464228471516 0.0 80-81 0.0011908898328831302 0.0 0.0 3.178239780764187 0.0 82-83 0.0011908898328831302 0.0 0.0 3.4311987917371862 0.0 84-85 0.0012259160044385165 0.0 0.0 3.6750509981057844 0.0 86-87 0.0012609421759939028 0.0 0.0 3.929621212970331 0.0 88-89 0.0012609421759939028 0.0 0.0 4.204331476479226 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3460 0.0 63.08635 1 GTATCAA 6620 0.0 44.538185 1 TGGTATC 1300 0.0 36.58099 2 GTGGTAT 1335 0.0 35.97816 1 ATCAACG 8515 0.0 34.28638 3 TATCAAC 8615 0.0 34.16915 2 TCAACGC 8685 0.0 33.778316 4 CAACGCA 8780 0.0 33.411667 5 AACGCAG 9015 0.0 32.55919 6 ACGCAGA 10285 0.0 28.354038 7 CGCAGAG 10285 0.0 28.2155 8 GCAGAGT 11045 0.0 25.929996 9 GAGTACT 7340 0.0 23.521185 12-13 GTACATG 6280 0.0 23.24757 1 CAGAGTA 10740 0.0 23.150654 10-11 TACATGG 6340 0.0 22.877544 2 GTACTTT 7740 0.0 22.152212 14-15 ACATGGG 6380 0.0 21.762064 3 AGAGTAC 10665 0.0 21.643438 10-11 AGTACTT 7600 0.0 21.52913 12-13 >>END_MODULE