FastQCFastQC Report
Thu 26 May 2016
SRR938118_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938118_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427300
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94060.6590065157990612No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83080.582078049464023No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51380.359980382540461No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19890.13935402508232325No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15650.10964758635185314No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34650.059.4899941
GTATCAA62950.044.873441
GTGGTAT13000.036.3375171
TGGTATC13150.036.1458662
TATCAAC80850.034.392222
ATCAACG81150.034.0893633
TCAACGC82300.033.6707764
CAACGCA83800.033.0113565
AACGCAG86750.031.941336
ACGCAGA97850.028.159387
CGCAGAG98450.027.939518
TACCGTA908.9411135E-426.3926757
GCAGAGT104800.025.47429
TACCTGG12450.024.8158072
GTACATG63900.024.0446471
GAGTACT69750.023.52998712-13
TACATGG62850.023.3688622
GTACTTT72150.022.58506614-15
CAGAGTA102400.022.45124610-11
GTACCTG14150.021.918981
Produced by FastQC (version 0.11.5)