##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938118_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1427300 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.840960554893854 33.0 31.0 34.0 30.0 34.0 2 32.028558116723886 34.0 31.0 34.0 30.0 34.0 3 32.09827226231346 34.0 31.0 34.0 30.0 34.0 4 35.6250325789953 37.0 35.0 37.0 33.0 37.0 5 35.640037133048416 37.0 35.0 37.0 33.0 37.0 6 35.66453934001261 37.0 35.0 37.0 33.0 37.0 7 35.6060828137042 37.0 35.0 37.0 33.0 37.0 8 35.58112800392349 37.0 35.0 37.0 33.0 37.0 9 37.20403068731171 39.0 37.0 39.0 34.0 39.0 10-11 37.11830484130877 39.0 37.0 39.0 33.5 39.0 12-13 37.01102641350802 39.0 37.0 39.0 33.0 39.0 14-15 38.17549288867092 40.0 38.0 41.0 33.0 41.0 16-17 38.024768443915086 40.0 38.0 41.0 33.0 41.0 18-19 38.18271456596371 40.0 38.0 41.0 33.5 41.0 20-21 38.17044559658096 40.0 38.0 41.0 33.0 41.0 22-23 38.07572059132628 40.0 38.0 41.0 33.0 41.0 24-25 37.96042738036853 40.0 38.0 41.0 33.0 41.0 26-27 37.77913613115673 40.0 38.0 41.0 32.5 41.0 28-29 37.56755342254607 40.0 37.5 41.0 32.0 41.0 30-31 37.33044069221607 40.0 37.0 41.0 31.5 41.0 32-33 37.05647901632453 40.0 36.0 41.0 31.0 41.0 34-35 36.804931689203386 39.0 36.0 41.0 30.5 41.0 36-37 36.53766271982064 39.0 35.0 41.0 30.0 41.0 38-39 36.24727002031808 39.0 35.0 40.5 30.0 41.0 40-41 36.0160656484271 39.0 35.0 40.0 29.0 41.0 42-43 35.821497933160515 39.0 35.0 40.0 28.5 41.0 44-45 35.51985146780635 39.0 35.0 40.0 27.0 41.0 46-47 35.08446157079801 38.5 35.0 40.0 25.0 41.0 48-49 34.66154697680936 38.0 34.0 40.0 23.5 41.0 50-51 33.95286309815736 37.5 33.5 39.5 23.0 40.5 52-53 34.075025572759756 38.0 33.5 40.0 23.0 40.5 54-55 34.18140439991592 38.0 34.0 40.0 22.5 41.0 56-57 33.87853464583479 38.0 33.5 40.0 21.0 41.0 58-59 33.55735199327401 37.5 33.0 40.0 19.5 41.0 60-61 33.135532824213556 37.0 33.0 40.0 17.5 41.0 62-63 32.596093673369296 36.5 32.0 39.5 12.5 41.0 64-65 32.07501891683599 36.0 31.0 39.0 8.0 40.5 66-67 31.53954809780705 35.0 31.0 39.0 6.0 40.0 68-69 30.935599383451272 35.0 30.0 38.0 2.0 40.0 70-71 30.383054018076088 35.0 29.5 37.5 2.0 40.0 72-73 29.74378021439081 34.5 29.0 37.0 2.0 39.0 74-75 29.056434877040566 34.0 28.0 36.0 2.0 39.0 76-77 28.38864919778603 34.0 26.5 36.0 2.0 38.5 78-79 27.654471379527777 33.5 26.0 35.0 2.0 37.0 80-81 26.995992783577385 33.0 25.0 35.0 2.0 37.0 82-83 26.270280249421987 32.5 23.0 35.0 2.0 36.0 84-85 25.602797239543193 32.0 20.5 35.0 2.0 36.0 86-87 24.90640545085126 31.5 18.5 34.5 2.0 35.0 88-89 24.095166047782527 31.0 9.5 34.0 2.0 35.0 90-91 23.268106915154487 30.5 3.5 34.0 2.0 35.0 92-93 22.36443074336159 30.0 2.0 34.0 2.0 35.0 94-95 21.40949204792265 29.0 2.0 34.0 2.0 35.0 96-97 20.20605759125622 28.0 2.0 34.0 2.0 35.0 98-99 18.728418342324666 26.0 2.0 33.5 2.0 35.0 100-101 15.142967140755271 10.0 2.0 29.5 2.0 33.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.21170076532527204 1101 2 0.3145803125570552 1101 3 0.3078223983569828 1101 4 0.19467194651736008 1101 5 0.23842397270993132 1101 6 0.2512981951430717 1101 7 0.24278868767706996 1101 8 0.22465051218831888 1101 9 0.2788567392001724 1101 10-11 0.3240825787962933 1101 12-13 0.35063575889059706 1101 14-15 0.47996860185215695 1101 16-17 0.5979564986696744 1101 18-19 0.39712917895257505 1101 20-21 0.496284977285228 1101 22-23 0.38908361235867517 1101 24-25 0.4511963267663859 1101 26-27 0.6084787788675285 1101 28-29 0.5854337392618518 1101 30-31 0.6516041813138784 1101 32-33 0.7104604520243996 1101 34-35 0.7570318527399849 1101 36-37 0.7153174028570675 1101 38-39 0.9708976702465932 1101 40-41 0.931000360945653 1101 42-43 1.0658349353820498 1101 44-45 1.0144224556076722 1101 46-47 1.1626337597850593 1101 48-49 1.1485288244953082 1101 50-51 0.9555071252571423 1101 52-53 1.185889039896395 1101 54-55 1.3073849470922525 1101 56-57 1.3116745080110732 1101 58-59 1.4461639464084826 1101 60-61 1.5658743523672953 1101 62-63 1.350488299683704 1101 64-65 1.2679424150151064 1101 66-67 1.3229488037129045 1101 68-69 1.6277490161030244 1101 70-71 1.711531066153963 1101 72-73 2.035561298162577 1101 74-75 2.0478528722573657 1101 76-77 2.0234407694383343 1101 78-79 2.352473876931967 1101 80-81 2.5077691108337845 1101 82-83 2.5583301743409805 1101 84-85 2.7484175217713087 1101 86-87 2.895460482938372 1101 88-89 3.1544207080912834 1101 90-91 3.4918336602199354 1101 92-93 3.738097756737634 1101 94-95 3.6589060457662903 1101 96-97 3.8707962988285267 1101 98-99 4.370360733140036 1101 100-101 4.305130627253085 1102 1 0.26519952792762425 1102 2 0.139065468399032 1102 3 0.07143238833599952 1102 4 -0.015130162133722536 1102 5 0.07266436188579206 1102 6 0.22374510515538049 1102 7 0.0680138829755137 1102 8 0.24089262504710263 1102 9 0.2637698999068405 1102 10-11 0.29248326310209904 1102 12-13 0.3301407260132905 1102 14-15 0.33910089174830915 1102 16-17 0.318491947666935 1102 18-19 0.09229936034528663 1102 20-21 0.3310218019069495 1102 22-23 0.3451564573397832 1102 24-25 0.13891351727394863 1102 26-27 0.20527446338557098 1102 28-29 0.24722555748896724 1102 30-31 0.18135801207922242 1102 32-33 0.179826324948003 1102 34-35 0.26894630344062165 1102 36-37 0.12863774397650474 1102 38-39 0.2643602244346468 1102 40-41 0.5093015182492522 1102 42-43 0.656235091879168 1102 44-45 0.591775956400312 1102 46-47 0.658100326057351 1102 48-49 0.7631537389109511 1102 50-51 0.6804865019630597 1102 52-53 0.5847601567654195 1102 54-55 0.6688997580315501 1102 56-57 0.6107750186567529 1102 58-59 0.5535351823112862 1102 60-61 0.6187438046089042 1102 62-63 0.6681313201519714 1102 64-65 0.8242287826024821 1102 66-67 0.815969179711999 1102 68-69 1.0577359316148716 1102 70-71 1.1657298211898244 1102 72-73 1.414085020948594 1102 74-75 1.1336522348268687 1102 76-77 1.3394819089883683 1102 78-79 1.5752691222245936 1102 80-81 1.9089812253350509 1102 82-83 1.8830487840264531 1102 84-85 1.8791728972075354 1102 86-87 2.1241428251540277 1102 88-89 2.678135189426971 1102 90-91 2.5794259113978164 1102 92-93 2.9401746654765617 1102 94-95 3.288727605828523 1102 96-97 3.508885631371996 1102 98-99 3.175164714958317 1102 100-101 3.1863948951584398 1103 1 0.41552160483568557 1103 2 0.4347552045923777 1103 3 0.3802249794826764 1103 4 0.20601557857087016 1103 5 0.26734991216376613 1103 6 0.27390596287040836 1103 7 0.32216618089123017 1103 8 0.4053651201852233 1103 9 0.3845346427875356 1103 10-11 0.34652061101023435 1103 12-13 0.5896215712934705 1103 14-15 0.6245882725509304 1103 16-17 0.6815315439682834 1103 18-19 0.6266133835232566 1103 20-21 0.6438137857705755 1103 22-23 0.7602976867987934 1103 24-25 0.7713572532214954 1103 26-27 0.7492464560692653 1103 28-29 0.7774780137320576 1103 30-31 0.8718796971451184 1103 32-33 1.173490338366065 1103 34-35 1.1541123302912055 1103 36-37 0.9499635007307177 1103 38-39 1.0050460205176748 1103 40-41 1.0955306519310497 1103 42-43 1.227807376637088 1103 44-45 1.2043203278262027 1103 46-47 1.334322663952534 1103 48-49 1.3223569690784558 1103 50-51 1.4591979774440063 1103 52-53 1.4802684309970928 1103 54-55 1.6515820694665493 1103 56-57 1.6223918375056599 1103 58-59 1.6181848016749427 1103 60-61 1.670189716654079 1103 62-63 1.7086685487723514 1103 64-65 1.9316376359219376 1103 66-67 2.072817742113955 1103 68-69 2.321158694608201 1103 70-71 2.5173980422119406 1103 72-73 2.5412341884647383 1103 74-75 2.6041574364954485 1103 76-77 2.9632523415329572 1103 78-79 3.2685586444229635 1103 80-81 3.1257653723553886 1103 82-83 3.158939646585285 1103 84-85 3.0052108813169625 1103 86-87 3.176446524519992 1103 88-89 3.476125010206175 1103 90-91 3.663968388678928 1103 92-93 3.8177001316024572 1103 94-95 4.385245488145742 1103 96-97 4.55661085024693 1103 98-99 4.800642408150235 1103 100-101 4.888269158646992 1104 1 0.22105021531374902 1104 2 0.1985234899115298 1104 3 0.10595980096342572 1104 4 -0.038429515152550664 1104 5 0.013964246944993874 1104 6 0.12717475787355426 1104 7 0.14914502088941362 1104 8 0.20644552040078423 1104 9 0.23681653374762135 1104 10-11 0.27425586101509936 1104 12-13 0.17660190463329428 1104 14-15 0.37478273160415654 1104 16-17 0.4026597311164579 1104 18-19 0.25354774791160395 1104 20-21 0.31752253220764715 1104 22-23 0.32268528037613464 1104 24-25 0.3801812791407073 1104 26-27 0.35818085941433964 1104 28-29 0.3469680700245448 1104 30-31 0.4369306330807561 1104 32-33 0.3404121716701809 1104 34-35 0.32156559843693344 1104 36-37 0.529515108670104 1104 38-39 0.48509172975756343 1104 40-41 0.545645898949239 1104 42-43 0.6848046561581782 1104 44-45 0.7350328465741214 1104 46-47 0.7852680141411454 1104 48-49 0.9174253706185453 1104 50-51 0.7976960003895215 1104 52-53 0.7925118846418258 1104 54-55 1.0141186725687987 1104 56-57 1.115535776161785 1104 58-59 1.028403803473445 1104 60-61 1.0457011861671859 1104 62-63 1.101741716020129 1104 64-65 1.1853511870234428 1104 66-67 1.257434699982241 1104 68-69 1.4482109031306365 1104 70-71 1.6965555378130794 1104 72-73 1.8240873799955395 1104 74-75 1.863370705160598 1104 76-77 1.8924299647823375 1104 78-79 2.1514839059200703 1104 80-81 2.3078257942568676 1104 82-83 2.235437695501961 1104 84-85 2.325052846814277 1104 86-87 2.4636297575397244 1104 88-89 2.823896684761582 1104 90-91 2.933659396814388 1104 92-93 3.2117059880988137 1104 94-95 3.4938818724409835 1104 96-97 3.8604714630364256 1104 98-99 3.9816425574844025 1104 100-101 3.824190842616943 1105 1 0.22632178415465276 1105 2 0.3096063509078135 1105 3 0.08485696518767583 1105 4 0.0825525757358605 1105 5 0.04801440258851386 1105 6 0.1637757040575707 1105 7 0.3161855145065928 1105 8 0.21242675160898017 1105 9 0.22385411468054883 1105 10-11 0.23089144768474767 1105 12-13 0.27810822023871395 1105 14-15 0.2758457152351568 1105 16-17 0.37761746553518805 1105 18-19 0.3239813725620664 1105 20-21 0.3688281502300583 1105 22-23 0.3305777779736587 1105 24-25 0.24698344894434854 1105 26-27 0.34125002982050034 1105 28-29 0.36405403171446693 1105 30-31 0.45119645399744 1105 32-33 0.5707752976720215 1105 34-35 0.5113021368919064 1105 36-37 0.5069780924959417 1105 38-39 0.6762495412309093 1105 40-41 0.7881558437202614 1105 42-43 0.8161525909043092 1105 44-45 0.8518969961555669 1105 46-47 0.645628914228773 1105 48-49 0.8295938153376952 1105 50-51 0.8665074352566933 1105 52-53 0.8799243153742466 1105 54-55 1.0886928873252018 1105 56-57 1.047160269790652 1105 58-59 1.0199363186250068 1105 60-61 1.4042713699244374 1105 62-63 1.3812690526333995 1105 64-65 1.2152059570796823 1105 66-67 1.2994014632350712 1105 68-69 1.5488098055649715 1105 70-71 1.4631338498102657 1105 72-73 1.573206633958712 1105 74-75 1.667877231667731 1105 76-77 1.7844141895285617 1105 78-79 2.003135003363969 1105 80-81 2.109755471927926 1105 82-83 2.1927829445389015 1105 84-85 2.465341455832199 1105 86-87 2.685662509164228 1105 88-89 2.8821040980787807 1105 90-91 3.1642020084595757 1105 92-93 3.151786520408912 1105 94-95 3.4103460393621425 1105 96-97 3.438100586761699 1105 98-99 3.7426390364485407 1105 100-101 3.8995451014922295 1106 1 0.12991694583653413 1106 2 0.122156579149717 1106 3 0.016911418921409904 1106 4 0.0042525593766598035 1106 5 0.10274190260162896 1106 6 -0.14438734808345544 1106 7 -0.013514453871970034 1106 8 -0.021192700868006398 1106 9 0.06607793625742886 1106 10-11 -0.056309883563379515 1106 12-13 0.08477342745450045 1106 14-15 0.1523699709748314 1106 16-17 -0.009876518999412554 1106 18-19 0.02823939202999526 1106 20-21 0.07993438060675828 1106 22-23 0.10376694999283131 1106 24-25 -0.10589102540293283 1106 26-27 0.04835731710672775 1106 28-29 0.12927536507068993 1106 30-31 0.01128695902944088 1106 32-33 0.15783297316741596 1106 34-35 0.3667945608633474 1106 36-37 0.4538500266355925 1106 38-39 0.04421454684216286 1106 40-41 0.39727459771169293 1106 42-43 0.2644210192140335 1106 44-45 0.3465905090871999 1106 46-47 0.7526646121109977 1106 48-49 0.6962095443071732 1106 50-51 0.6691355438035913 1106 52-53 0.6124236631523345 1106 54-55 0.2980010360690031 1106 56-57 0.7432126936476209 1106 58-59 0.8476328806642996 1106 60-61 0.9284987313851119 1106 62-63 0.7914436668685738 1106 64-65 0.9422182942090203 1106 66-67 1.0752505560146517 1106 68-69 0.8461683902601003 1106 70-71 0.9725456386439397 1106 72-73 1.1459707528343266 1106 74-75 1.2629451939070613 1106 76-77 1.6067680167000056 1106 78-79 1.8226285929883197 1106 80-81 1.9704403931771992 1106 82-83 1.7385679693328733 1106 84-85 1.9124606858144695 1106 86-87 1.7080581643885147 1106 88-89 2.1175082777362313 1106 90-91 2.3847050039109554 1106 92-93 2.645318434450118 1106 94-95 2.6950889589983866 1106 96-97 2.759966499474139 1106 98-99 2.95508047359748 1106 100-101 3.0465521063794796 1107 1 0.061280006509939255 1107 2 -0.01619264540640586 1107 3 -0.04826649108408532 1107 4 0.029122920453914958 1107 5 0.05972209234043646 1107 6 0.08369351792844526 1107 7 -0.028533451649593644 1107 8 -0.016604558348831233 1107 9 -0.10360567308134705 1107 10-11 -0.07655327018608205 1107 12-13 -0.015060491344932814 1107 14-15 0.005928768499146031 1107 16-17 0.09120898294816016 1107 18-19 -0.017758528967924292 1107 20-21 0.1918091864470881 1107 22-23 0.1026826428740435 1107 24-25 0.18951545534837066 1107 26-27 0.21229977415263335 1107 28-29 0.183729686766398 1107 30-31 0.3947254372955129 1107 32-33 0.1639893284617031 1107 34-35 0.0914641907053877 1107 36-37 0.1455065372576243 1107 38-39 0.21795758696450918 1107 40-41 0.31798277273021114 1107 42-43 0.38101838581140157 1107 44-45 0.3443472180508067 1107 46-47 0.07186312096467873 1107 48-49 0.4248221091266444 1107 50-51 0.13188675655480608 1107 52-53 0.02847798006872182 1107 54-55 0.02027503420506349 1107 56-57 0.26236579900761825 1107 58-59 0.268932495929441 1107 60-61 0.29433738170651935 1107 62-63 0.4972499373300181 1107 64-65 0.7001639056029063 1107 66-67 0.8615524414544709 1107 68-69 0.8841968032885141 1107 70-71 0.9983151083789288 1107 72-73 1.2539145719850424 1107 74-75 1.3784164343783019 1107 76-77 1.5495924724554975 1107 78-79 1.3785256704371243 1107 80-81 1.4321521048889068 1107 82-83 1.636666996742246 1107 84-85 1.7456386914062705 1107 86-87 1.7503222454456981 1107 88-89 2.0549918488404906 1107 90-91 2.000062735647994 1107 92-93 2.218486761928787 1107 94-95 2.6781761576028273 1107 96-97 2.820568215248265 1107 98-99 3.161543899888489 1107 100-101 3.2722433148293106 1108 1 0.25315066541251596 1108 2 0.23685792572565845 1108 3 0.16634246580510137 1108 4 0.15198301605551734 1108 5 0.15749846291011238 1108 6 0.20303005877222802 1108 7 0.19202158510626077 1108 8 0.09778297983203288 1108 9 0.31740995963387064 1108 10-11 0.18135902686842797 1108 12-13 0.322497786994667 1108 14-15 0.33389201957722037 1108 16-17 0.32575580226322387 1108 18-19 0.4197320503417288 1108 20-21 0.2643963635664619 1108 22-23 0.14876627781384855 1108 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2817.0 9 3358.0 10 4378.0 11 5489.0 12 6715.0 13 8475.0 14 9805.0 15 12039.0 16 14799.0 17 18317.0 18 17799.0 19 14264.0 20 14233.0 21 15272.0 22 16467.0 23 18213.0 24 20666.0 25 23032.0 26 25697.0 27 29755.0 28 34218.0 29 39649.0 30 46980.0 31 56310.0 32 68175.0 33 86593.0 34 111728.0 35 152683.0 36 195492.0 37 219326.0 38 115975.0 39 6704.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.596893566612636 18.435605945961544 14.343481409895906 22.624019077529915 2 15.771659194836726 19.69177650116493 40.52178045436576 24.014783849632575 3 19.433818493570804 24.311638167621076 29.759158354591076 26.495384984217047 4 12.78507314528951 16.462316926467743 36.32504157097631 34.42756835726644 5 13.659130032359965 37.2091898088904 33.869978157901194 15.26170200084844 6 32.17335451905102 35.53590937075333 17.153769884399466 15.136966225796183 7 28.63579586635395 33.166460353531455 20.006953430242095 18.190790349872497 8 26.785413898094102 34.82037220829759 19.209267638961318 19.184946254646995 9 26.877898402409006 16.58615159450684 20.03367333778199 36.50227666530216 10-11 25.429008138721017 26.568134669353288 26.37452416643914 21.62833302548656 12-13 26.232251091202595 24.11433321861132 27.323944353048464 22.32947133713762 14-15 23.048668471032773 25.281089656762745 26.043430248797154 25.62681162340732 16-17 22.003801692397946 28.573075691106887 26.800634736842845 22.622487879652326 18-19 21.922670360312747 26.561761790670644 29.770348115861978 21.745219733154634 20-21 22.668029922459862 25.664131534321726 30.220359526585238 21.44747901663317 22-23 22.456256810366792 25.704378997941603 30.05547831249757 21.783885879194038 24-25 22.11154819852758 25.931198343196428 30.14072173874636 21.816531719529628 26-27 21.709031780232092 26.24863430212444 30.03511819574004 22.007215721903428 28-29 21.728956158744367 26.378842589169025 30.12984536944195 21.762355882644655 30-31 21.90382763934611 26.022150702018347 30.07486490076484 21.999156757870697 32-33 21.654144772189373 26.157295368925958 30.064077379314746 22.124482479569924 34-35 22.313909970762264 26.274358063089302 29.690602917044316 21.721129049104118 36-37 22.300031227186366 25.994337049637206 29.675885637702255 22.029746085474173 38-39 21.93854327526015 26.242469285829166 29.741857647995857 22.07712979091482 40-41 22.193866824394785 26.1006161488176 29.776905351916845 21.928611674870773 42-43 22.075851982252896 26.067408722319207 29.573414944037474 22.283324351390426 44-45 22.018603846130937 26.427180717420757 29.56472718061231 21.989488255835994 46-47 22.32107972907958 26.67509277892458 28.92876828202971 22.075059209966128 48-49 22.302355406361908 26.723315089037303 28.75144541077698 22.2228840938238 50-51 22.331155676424896 26.877677253167153 28.56760955496896 22.223557515438987 52-53 22.42451380122356 26.847996815843423 28.59434272638502 22.133146656548 54-55 22.251645512496644 26.860481982925773 28.573020252084174 22.31485225249341 56-57 22.264791918274593 27.179051645274953 28.453304547109237 22.102851889341213 58-59 22.31838195646149 26.717850382055964 28.490454119123537 22.473313542359 60-61 22.20054959782235 26.83951119920311 28.732480824866236 22.227458378108306 62-63 22.13329885713505 26.846692684984603 28.823117832568308 22.196890625312047 64-65 21.95069908079766 26.86064458035006 28.65665418756493 22.532002151287355 66-67 21.95300287075439 27.074780454205616 28.42162462136102 22.550592053678972 68-69 22.04433363779469 26.797154109650233 28.586398880815466 22.57211337173961 70-71 22.010565362890468 26.8664137155607 28.445331762695936 22.6776891588529 72-73 22.101892572940578 26.914120858945573 28.288983162491625 22.69500340562222 74-75 21.97188911627333 27.157971226232807 28.198999578515426 22.67114007897843 76-77 22.175750687730442 26.973012098169928 28.197788892330074 22.65344832176956 78-79 22.254798123039503 27.014223655125853 28.065660683297427 22.665317538537217 80-81 22.156854501052866 27.180942570538626 27.944157723423412 22.718045204985092 82-83 22.273535021715578 27.146647083637564 27.935176997041523 22.64464089760533 84-85 22.265121498575276 26.992524431274134 27.860008527690994 22.882345542459596 86-87 22.376455033776647 26.856944873106798 27.889249451827947 22.87735064128861 88-89 22.296366093726842 26.981726921070127 27.761820278572735 22.960086706630303 90-91 22.35454736915142 26.9930100316751 27.725998287813443 22.92644431136004 92-93 22.440197372408857 26.656241886663278 27.765068951756017 23.138491789171848 94-95 22.317336976881016 26.651817738045136 27.785356821086417 23.245488463987435 96-97 22.463354987509632 26.428805953047146 27.682993791784217 23.424845267659006 98-99 22.431737789013923 26.501908801510922 27.372651666062225 23.693701743412927 100-101 23.153004887430523 25.55139274754567 27.44781121628643 23.847791148737375 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1539.0 1 1049.0 2 816.5 3 1085.0 4 850.5 5 670.5 6 1197.5 7 2671.5 8 4320.5 9 4843.0 10 3789.5 11 2535.5 12 2254.5 13 2371.0 14 2447.0 15 2567.0 16 2615.5 17 2566.5 18 2729.5 19 2961.5 20 3232.0 21 3688.5 22 4265.0 23 5035.5 24 6112.0 25 7355.5 26 8840.5 27 10533.0 28 12014.5 29 13548.0 30 15642.5 31 18150.5 32 20969.0 33 23041.0 34 24843.0 35 26982.0 36 29636.5 37 32323.0 38 34205.0 39 35800.0 40 37023.0 41 39476.0 42 41955.0 43 43998.5 44 46774.5 45 49618.0 46 50994.5 47 51773.0 48 52875.0 49 52620.5 50 52117.0 51 51141.0 52 49806.0 53 49761.5 54 49575.5 55 46736.5 56 42828.0 57 39260.0 58 35934.0 59 32221.5 60 27162.5 61 22373.0 62 18659.0 63 14824.0 64 11349.0 65 8834.0 66 6680.5 67 5207.0 68 3982.5 69 2774.5 70 2038.0 71 1547.0 72 1158.5 73 882.0 74 647.5 75 496.5 76 339.5 77 206.0 78 119.5 79 66.5 80 38.0 81 20.5 82 14.0 83 13.5 84 12.0 85 11.5 86 7.5 87 3.0 88 3.0 89 3.5 90 4.0 91 2.0 92 0.5 93 0.0 94 0.5 95 1.5 96 1.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.46037973796679044 2 0.04259791214180621 3 0.053317452532754155 4 0.05773138092902683 5 0.08092202059833252 6 0.0828137041967351 7 0.046661528760596926 8 0.040075667343936104 9 0.045680655783647446 10-11 0.05076017655713585 12-13 0.04645134169410775 14-15 0.040320885588173476 16-17 0.039725355566454144 18-19 0.049464022980452604 20-21 0.05696069501856652 22-23 0.0494990541582008 24-25 0.06578855181111189 26-27 0.05825684859524977 28-29 0.04494500105093533 30-31 0.04648637287185595 32-33 0.04662649758284873 34-35 0.05037483360190569 36-37 0.046136061094373994 38-39 0.027569536887830166 40-41 0.026063196244657746 42-43 0.025432635045190218 44-45 0.016079310586421914 46-47 0.02707910039935543 48-49 0.025923071533664962 50-51 0.016184404119666502 52-53 0.016429622363903874 54-55 0.016604778252644854 56-57 0.016009248230925525 58-59 0.015308624675961606 60-61 0.017970994184824493 62-63 0.016009248230925525 64-65 0.017340432985356966 66-67 0.010439290968962376 68-69 0.013276816366566245 70-71 0.019512366005745116 72-73 0.01737546416310516 74-75 0.014117564632522945 76-77 0.009948854480487634 78-79 0.014397814054508512 80-81 0.01716527709661599 82-83 0.014993344076227842 84-85 0.012926504589084285 86-87 0.003958523085546136 88-89 0.029391158130736356 90-91 0.03289427590555594 92-93 0.017620682407342533 94-95 0.034015273593498214 96-97 0.02805997337630491 98-99 0.025047292089960062 100-101 0.14807678834162402 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1427300.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.564991739536026 #Duplication Level Percentage of deduplicated Percentage of total 1 81.99247729774338 51.29858664837409 2 10.834792700900943 13.557574316629054 3 3.011999308975758 5.653371356566696 4 1.3041337390599286 3.263724664461386 5 0.6825858878180855 2.135299021643119 6 0.4494675722819846 1.6872560968207007 7 0.2863509957210457 1.2540883377326597 8 0.23279523785616338 1.165186570677935 9 0.16837327093840226 0.948084507487782 >10 0.9481671444343304 10.8529923080061 >50 0.06224900769227608 2.6473609698578833 >100 0.02436250115971747 2.884926680012649 >500 0.0014594680217007775 0.6315251061953375 >1k 4.490670836002392E-4 0.4149058839167899 >5k 3.3680031270017944E-4 1.6051175316178234 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9406 0.6590065157990612 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8308 0.582078049464023 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5138 0.359980382540461 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1989 0.13935402508232325 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1565 0.10964758635185314 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 7.006235549639179E-5 2 0.0 0.0 0.0 0.0 7.006235549639179E-5 3 0.0 0.0 0.0 0.0 7.006235549639179E-5 4 0.0 0.0 0.0 0.0 7.006235549639179E-5 5 0.0 0.0 0.0 0.0 7.006235549639179E-5 6 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 7 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 8 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 9 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 10-11 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 12-13 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 14-15 1.4012471099278357E-4 0.0 0.0 0.0 7.006235549639179E-5 16-17 1.4012471099278357E-4 0.0 0.0 7.006235549639179E-5 7.006235549639179E-5 18-19 2.452182442373713E-4 0.0 0.0 7.006235549639179E-5 7.006235549639179E-5 20-21 2.8024942198556714E-4 0.0 0.0 7.006235549639179E-5 7.006235549639179E-5 22-23 3.5031177748195893E-4 0.0 0.0 3.5031177748195893E-4 7.006235549639179E-5 24-25 4.203741329783507E-4 0.0 0.0 7.706859104603097E-4 7.006235549639179E-5 26-27 4.203741329783507E-4 0.0 0.0 0.001261122398935052 7.006235549639179E-5 28-29 6.30561199467526E-4 0.0 0.0 0.0032228683528340227 7.006235549639179E-5 30-31 7.706859104603097E-4 0.0 0.0 0.01015904154697681 7.006235549639179E-5 32-33 7.706859104603097E-4 0.0 0.0 0.02704406922160723 7.006235549639179E-5 34-35 8.057170882085056E-4 0.0 0.0 0.051916205422826316 7.006235549639179E-5 36-37 8.407482659567014E-4 0.0 0.0 0.095494990541582 7.006235549639179E-5 38-39 9.108106214530933E-4 0.0 0.0 0.16187907237441324 7.006235549639179E-5 40-41 9.80872976949485E-4 0.0 0.0 0.22426959994395013 7.006235549639179E-5 42-43 9.80872976949485E-4 0.0 0.0 0.2863448469137533 7.006235549639179E-5 44-45 0.0010509353324458768 0.0 0.0 0.3606810060954249 7.006235549639179E-5 46-47 0.0011560288656904645 0.0 0.0 0.45579065368177674 7.006235549639179E-5 48-49 0.0011910600434386603 0.0 0.0 0.5573810691515448 7.006235549639179E-5 50-51 0.0011910600434386603 0.0 0.0 0.6620542282631542 7.006235549639179E-5 52-53 0.0011910600434386603 0.0 0.0 0.7604918377355847 7.006235549639179E-5 54-55 0.0011910600434386603 0.0 0.0 0.8682477404890352 7.006235549639179E-5 56-57 0.0011910600434386603 0.0 0.0 0.997652911090871 7.006235549639179E-5 58-59 0.0011910600434386603 0.0 0.0 1.1338891613536046 7.006235549639179E-5 60-61 0.0011910600434386603 0.0 0.0 1.282036012050725 7.006235549639179E-5 62-63 0.0011910600434386603 0.0 0.0 1.4220906606880122 7.006235549639179E-5 64-65 0.0011910600434386603 0.0 0.0 1.5635115252574792 7.006235549639179E-5 66-67 0.0011910600434386603 0.0 0.0 1.731731240804316 7.006235549639179E-5 68-69 0.0011910600434386603 0.0 0.0 1.9183773558467037 7.006235549639179E-5 70-71 0.0011910600434386603 0.0 0.0 2.114797169480838 7.006235549639179E-5 72-73 0.0011910600434386603 0.0 0.0 2.3058572129194985 7.006235549639179E-5 74-75 0.0011910600434386603 0.0 0.0 2.4973025993133886 7.006235549639179E-5 76-77 0.0011910600434386603 0.0 0.0 2.702094864429342 7.006235549639179E-5 78-79 0.0011910600434386603 0.0 0.0 2.9242625937084004 7.006235549639179E-5 80-81 0.001331184754431444 0.0 0.0 3.170636866811462 7.006235549639179E-5 82-83 0.0014012471099278357 0.0 0.0 3.4156799551600923 7.006235549639179E-5 84-85 0.0014012471099278357 0.0 0.0 3.656484271001191 7.006235549639179E-5 86-87 0.0014012471099278357 0.0 0.0 3.9102851537868704 7.006235549639179E-5 88-89 0.0014012471099278357 0.0 0.0 4.186155678553913 7.006235549639179E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3465 0.0 59.489994 1 GTATCAA 6295 0.0 44.87344 1 GTGGTAT 1300 0.0 36.337517 1 TGGTATC 1315 0.0 36.145866 2 TATCAAC 8085 0.0 34.39222 2 ATCAACG 8115 0.0 34.089363 3 TCAACGC 8230 0.0 33.670776 4 CAACGCA 8380 0.0 33.011356 5 AACGCAG 8675 0.0 31.94133 6 ACGCAGA 9785 0.0 28.15938 7 CGCAGAG 9845 0.0 27.93951 8 TACCGTA 90 8.9411135E-4 26.392675 7 GCAGAGT 10480 0.0 25.4742 9 TACCTGG 1245 0.0 24.815807 2 GTACATG 6390 0.0 24.044647 1 GAGTACT 6975 0.0 23.529987 12-13 TACATGG 6285 0.0 23.368862 2 GTACTTT 7215 0.0 22.585066 14-15 CAGAGTA 10240 0.0 22.451246 10-11 GTACCTG 1415 0.0 21.91898 1 >>END_MODULE