FastQCFastQC Report
Thu 26 May 2016
SRR938118_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938118_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1427300
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT91940.6441532964338261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT86130.6034470678904225No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT53370.37392279128424294No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21300.14923281720731452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17220.12064737616478666No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA35000.062.8406751
GTATCAA62250.041.2080731
TGGTATC13250.033.342252
ATCAACG81450.031.4908893
TATCAAC81600.031.4363022
TCAACGC82450.031.2241654
GTGGTAT14650.031.1287361
CAACGCA83150.030.904185
AACGCAG86650.029.820346
ACGCAGA99700.025.6788657
CGCAGAG100300.025.5252558
GCAGAGT107250.023.3397169
GAGTACT70700.022.77521712-13
GTACATG59350.022.4912451
CAGAGTA103800.021.94186210-11
TACATGG60850.021.6245482
GTACTTT74900.021.30785414-15
ACATGGG60250.020.9704063
TAAGGTG6350.020.9443345
GTGTAAG5800.020.4757541
Produced by FastQC (version 0.11.5)