FastQCFastQC Report
Thu 26 May 2016
SRR938117_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938117_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429547
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT97120.6793760540926601No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85800.6001901301601137No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51500.360253982555313No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20890.14613020768117452No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16380.11458175212147624No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34000.062.4973871
GTATCAA62850.044.5218351
GTGGTAT13250.037.8402631
TGGTATC13300.035.7281232
TATCAAC81900.033.8836982
ATCAACG82500.033.5220763
TCAACGC83550.032.873294
CAACGCA84900.032.3505745
AACGCAG87800.031.3361666
ACGCAGA99450.027.6301087
CGCAGAG100700.027.1927158
GCAGAGT105700.025.3217079
GTACATG61750.024.9773461
TACATGG62800.024.364532
ACATGGG63600.023.3109133
GAGTACT69150.023.30188212-13
CAGAGTA103350.022.5383610-11
GTACTTT73700.021.6584414-15
AGAGTAC103050.020.62036110-11
AGTACTT72050.020.48382812-13
Produced by FastQC (version 0.11.5)