##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938117_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429547 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.877550720612895 33.0 31.0 34.0 30.0 34.0 2 32.06257926462019 34.0 31.0 34.0 30.0 34.0 3 32.12349156760848 34.0 31.0 34.0 30.0 34.0 4 35.65020317625094 37.0 35.0 37.0 33.0 37.0 5 35.67317408941434 37.0 35.0 37.0 35.0 37.0 6 35.70156839894037 37.0 35.0 37.0 35.0 37.0 7 35.64302118083561 37.0 35.0 37.0 33.0 37.0 8 35.627861133631846 37.0 35.0 37.0 33.0 37.0 9 37.28335409748683 39.0 37.0 39.0 34.0 39.0 10-11 37.20300381869221 39.0 37.0 39.0 34.0 39.0 12-13 37.10235759999496 39.0 37.0 39.0 33.5 39.0 14-15 38.335393659669805 40.0 38.0 41.0 33.5 41.0 16-17 38.256730978414836 40.0 38.0 41.0 33.0 41.0 18-19 38.21478972010014 40.0 38.0 41.0 33.5 41.0 20-21 38.12635331332233 40.0 38.0 41.0 33.0 41.0 22-23 37.97198658036427 40.0 38.0 41.0 33.0 41.0 24-25 37.91262896567934 40.0 38.0 41.0 33.0 41.0 26-27 37.91639064682728 40.0 38.0 41.0 33.0 41.0 28-29 37.81988699916827 40.0 38.0 41.0 33.0 41.0 30-31 37.61416973348901 40.0 37.5 41.0 32.5 41.0 32-33 37.371534828865364 40.0 37.0 41.0 32.0 41.0 34-35 37.151402507227814 40.0 36.0 41.0 31.0 41.0 36-37 36.93435263058857 40.0 36.0 41.0 31.0 41.0 38-39 36.690290700480645 39.5 35.5 41.0 30.0 41.0 40-41 36.45534914207088 39.0 35.0 41.0 30.0 41.0 42-43 36.15768211888101 39.0 35.0 41.0 29.5 41.0 44-45 35.89963289069894 39.0 35.0 41.0 28.5 41.0 46-47 35.694778835533214 39.0 35.0 41.0 27.5 41.0 48-49 35.402137180519425 39.0 35.0 41.0 26.0 41.0 50-51 34.75050173236697 38.5 34.5 40.0 25.0 40.5 52-53 34.818716698366686 38.5 34.5 40.0 25.5 41.0 54-55 34.95822767631984 39.0 35.0 40.0 25.5 41.0 56-57 34.752390092805626 38.5 35.0 40.0 24.5 41.0 58-59 34.49641949512678 38.0 34.0 40.0 23.5 41.0 60-61 34.12862186412899 38.0 34.0 40.0 22.5 41.0 62-63 33.71313220201924 37.0 33.5 40.0 22.0 41.0 64-65 33.28945078405957 36.5 33.0 40.0 20.0 41.0 66-67 32.85729045634736 36.0 33.0 39.0 18.5 41.0 68-69 32.379357236942894 35.5 32.0 39.0 16.0 40.5 70-71 31.903537274395315 35.0 31.5 38.5 12.0 40.0 72-73 31.411244261293962 35.0 31.0 37.5 7.5 40.0 74-75 30.896582973487405 35.0 31.0 37.0 6.0 39.0 76-77 30.36001929282493 35.0 30.0 36.0 2.0 39.0 78-79 29.80809725038771 34.0 29.5 36.0 2.0 38.5 80-81 29.279972606706878 34.0 29.0 35.0 2.0 37.0 82-83 28.74648892271468 34.0 29.0 35.0 2.0 37.0 84-85 28.25655644760193 34.0 27.5 35.0 2.0 36.0 86-87 27.751724847101915 34.0 26.5 35.0 2.0 36.0 88-89 27.207103718870385 33.0 26.0 35.0 2.0 35.5 90-91 26.609175843816256 33.0 25.0 35.0 2.0 35.0 92-93 25.995945568771084 32.5 23.5 35.0 2.0 35.0 94-95 25.390623393284727 32.0 20.5 35.0 2.0 35.0 96-97 24.56592333095729 32.0 12.5 34.0 2.0 35.0 98-99 23.50495471642415 31.0 2.0 34.0 2.0 35.0 100-101 20.68685674552848 28.0 2.0 32.5 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.4309336833528512 1101 2 0.3227173110245758 1101 3 0.3468200779021018 1101 4 0.2699467658480401 1101 5 0.31107332532318566 1101 6 0.3331980577290352 1101 7 0.3090938821909859 1101 8 0.32161605738699706 1101 9 0.3732087341713708 1101 10-11 0.37526196064720097 1101 12-13 0.21362509304255184 1101 14-15 0.37035380122370753 1101 16-17 0.5572894795221188 1101 18-19 0.4363320304596954 1101 20-21 0.5736992192794617 1101 22-23 0.449182358236456 1101 24-25 0.4128946649851528 1101 26-27 0.42863824743933066 1101 28-29 0.4411344648934161 1101 30-31 0.5883324762524111 1101 32-33 0.5580196063666278 1101 34-35 0.5415361749460033 1101 36-37 0.3709168725120122 1101 38-39 0.5062602465899531 1101 40-41 0.5219232277416026 1101 42-43 0.41861279111638083 1101 44-45 0.5056613909931755 1101 46-47 0.5413812296372882 1101 48-49 0.5352505065225657 1101 50-51 0.5058468798986198 1101 52-53 0.3616025311068469 1101 54-55 0.2900598522558795 1101 56-57 0.2620019122975066 1101 58-59 0.29083570370814726 1101 60-61 0.2121440061667954 1101 62-63 0.39159982680683214 1101 64-65 0.3243580348483519 1101 66-67 0.3403859538004994 1101 68-69 0.3728027136903549 1101 70-71 0.4140838715489821 1101 72-73 0.5118659028786716 1101 74-75 0.5476314903814057 1101 76-77 0.41964622114473826 1101 78-79 0.5718970671579235 1101 80-81 0.49231242798660446 1101 82-83 0.5450514672177356 1101 84-85 0.5375638088842969 1101 86-87 0.4578883871125541 1101 88-89 0.2998676482267264 1101 90-91 0.4402452215430266 1101 92-93 0.3231607209073992 1101 94-95 0.5539768402158423 1101 96-97 0.5699797964184015 1101 98-99 0.9779613552015469 1101 100-101 0.8050129539732289 1102 1 0.4458975862187451 1102 2 0.2533476131709591 1102 3 0.32119475886177185 1102 4 0.11347723571848434 1102 5 0.2017354707326504 1102 6 0.19130350511472471 1102 7 0.2657347825531744 1102 8 0.20457140389518003 1102 9 0.23296711429728134 1102 10-11 0.2823239942406346 1102 12-13 0.1803415907697712 1102 14-15 0.29328155833982095 1102 16-17 0.2843738763232082 1102 18-19 0.05187506748053039 1102 20-21 0.2703356226922793 1102 22-23 0.2616881313471282 1102 24-25 0.3127439564117793 1102 26-27 0.3556193359441622 1102 28-29 0.29056870004740887 1102 30-31 0.41147657309482355 1102 32-33 0.4409050678508635 1102 34-35 0.4471743771385732 1102 36-37 0.6123064298663508 1102 38-39 0.3793648432014436 1102 40-41 0.49537542638521614 1102 42-43 0.6254366079325422 1102 44-45 0.5547844858666551 1102 46-47 0.7931362552820502 1102 48-49 0.6258464130832522 1102 50-51 0.823401390217299 1102 52-53 0.8950871807094174 1102 54-55 0.8706117616362761 1102 56-57 0.7902628082842824 1102 58-59 0.861729502779589 1102 60-61 0.794402325523123 1102 62-63 0.8850482577361092 1102 64-65 0.9937016017799678 1102 66-67 0.7970019386324125 1102 68-69 0.8811026237513673 1102 70-71 1.052945414124153 1102 72-73 0.9363870385406976 1102 74-75 1.0647873522156708 1102 76-77 0.9268863096252531 1102 78-79 0.9210894393594806 1102 80-81 1.0720475941306553 1102 82-83 1.226911745785518 1102 84-85 1.248526034509613 1102 86-87 1.023562078743364 1102 88-89 1.0127447235080922 1102 90-91 1.276739788742038 1102 92-93 1.3554050632589423 1102 94-95 1.397475709901542 1102 96-97 1.1884871738461484 1102 98-99 1.33503797349562 1102 100-101 1.4298233528647906 1103 1 0.40531565666284664 1103 2 0.32072893016943027 1103 3 0.2300768415570289 1103 4 0.15635625562659783 1103 5 0.11214894699605082 1103 6 0.15378436269512008 1103 7 0.2856428989390878 1103 8 0.20227431354295788 1103 9 0.13648931950402243 1103 10-11 0.25246181966176806 1103 12-13 0.15775353563959982 1103 14-15 0.22819733169357903 1103 16-17 0.26178582119303684 1103 18-19 0.16711243348359517 1103 20-21 0.146292743672376 1103 22-23 0.395302220167963 1103 24-25 0.4157301738696617 1103 26-27 0.3280542517175107 1103 28-29 0.33229917477941484 1103 30-31 0.29891914583601675 1103 32-33 0.30039970797337645 1103 34-35 0.3809416053162167 1103 36-37 0.4247107177683347 1103 38-39 0.3556282428951647 1103 40-41 0.35142443097939946 1103 42-43 0.2789588131392833 1103 44-45 0.3557033220075496 1103 46-47 0.4424471281763829 1103 48-49 0.28893982809090346 1103 50-51 0.3199557546889693 1103 52-53 0.24807339816729979 1103 54-55 0.33041268047241346 1103 56-57 0.03834856632410322 1103 58-59 0.16035124856826144 1103 60-61 0.1707422948952484 1103 62-63 0.21238363292753348 1103 64-65 0.20426821740018397 1103 66-67 0.19478141795859472 1103 68-69 0.10506893309287335 1103 70-71 0.2409239746142049 1103 72-73 0.5730639144978191 1103 74-75 0.3806372756459986 1103 76-77 0.3250486373434782 1103 78-79 0.4685626399720384 1103 80-81 0.3733492786635786 1103 82-83 0.2919959724317671 1103 84-85 0.5601646256275323 1103 86-87 0.5162879592946688 1103 88-89 0.484413942497369 1103 90-91 0.521379911253522 1103 92-93 0.697288677347764 1103 94-95 0.8848417129643273 1103 96-97 0.9047965153469129 1103 98-99 1.2274575753332932 1103 100-101 0.9715538275967965 1104 1 0.1528536171482351 1104 2 0.18999660024449128 1104 3 0.0662504423722936 1104 4 0.11325101972434481 1104 5 0.028067566901064822 1104 6 0.0663934647839639 1104 7 0.15068842490790502 1104 8 0.1016730669154029 1104 9 -0.159971730870069 1104 10-11 0.09149444688330988 1104 12-13 0.04644694976013852 1104 14-15 -0.0336291074116275 1104 16-17 -0.09559691519989855 1104 18-19 0.11180040511344203 1104 20-21 0.15154394877154687 1104 22-23 0.13676755644801375 1104 24-25 0.24291834232960952 1104 26-27 0.18589690518676605 1104 28-29 0.35175679975543517 1104 30-31 0.30490000840647014 1104 32-33 0.21914824560895596 1104 34-35 0.302364537186385 1104 36-37 0.32518482878938215 1104 38-39 0.21302955339546514 1104 40-41 0.20031880030806093 1104 42-43 0.23814401417765652 1104 44-45 0.298379745532678 1104 46-47 0.4664487560444286 1104 48-49 0.5327870857209831 1104 50-51 0.340046562668185 1104 52-53 0.44759008326021643 1104 54-55 0.45897960214956157 1104 56-57 0.45951090624024005 1104 58-59 0.4529278154744176 1104 60-61 0.451132029568285 1104 62-63 0.4764655785625109 1104 64-65 0.49689662547165625 1104 66-67 0.4678565582624188 1104 68-69 0.6164368412491754 1104 70-71 0.7640387581620871 1104 72-73 0.612751791871009 1104 74-75 0.5579118830115455 1104 76-77 0.7761793291984844 1104 78-79 0.6641895743128465 1104 80-81 0.8012997617567059 1104 82-83 0.6920015723865234 1104 84-85 0.6560588607790585 1104 86-87 0.6431377463300514 1104 88-89 0.6778327758660012 1104 90-91 0.5327489283117579 1104 92-93 0.5504919623588478 1104 94-95 0.6273208628357523 1104 96-97 0.5771539743203107 1104 98-99 1.0305994826224385 1104 100-101 0.9183254259569544 1105 1 0.24843091480356705 1105 2 0.15723189205078825 1105 3 0.2212386825532633 1105 4 0.06742319123144824 1105 5 0.07510735331070606 1105 6 0.13690288003850526 1105 7 0.06751698363734704 1105 8 0.18761491640911032 1105 9 0.3420365471852307 1105 10-11 0.32369563291808134 1105 12-13 0.018601770112255167 1105 14-15 0.12414000210292642 1105 16-17 0.30912762845328956 1105 18-19 0.2118471484166804 1105 20-21 0.20935561714516382 1105 22-23 0.18142756780724767 1105 24-25 0.16069324883024905 1105 26-27 0.14369831656917142 1105 28-29 0.26674522364371 1105 30-31 0.3228311171464 1105 32-33 0.292013196755569 1105 34-35 0.2829553500861124 1105 36-37 0.28317147837127266 1105 38-39 0.15519645250290637 1105 40-41 0.09918588756239188 1105 42-43 0.06594239949249925 1105 44-45 0.14067850566146234 1105 46-47 0.0528396678399119 1105 48-49 0.11876924876223427 1105 50-51 0.11916863566224123 1105 52-53 0.09621694769800371 1105 54-55 0.17444607327001194 1105 56-57 0.13281553020414805 1105 58-59 0.09710437044193299 1105 60-61 0.1742157199826906 1105 62-63 0.15130568941966516 1105 64-65 0.06556998826960125 1105 66-67 0.2613136694956637 1105 68-69 0.3041997404362249 1105 70-71 0.32042720521789647 1105 72-73 0.09753572401361055 1105 74-75 0.09545272599333643 1105 76-77 0.01596992328239466 1105 78-79 -0.06361335265482282 1105 80-81 -0.04409063923041501 1105 82-83 0.07058252530693565 1105 84-85 0.2218661344656887 1105 86-87 0.14444666088013136 1105 88-89 0.3124955887616423 1105 90-91 0.10626757128630615 1105 92-93 0.1115093232094928 1105 94-95 0.6088595544008832 1105 96-97 0.3834894779513398 1105 98-99 0.33954311969028694 1105 100-101 0.4492568883334407 1106 1 0.15410379945582875 1106 2 0.1960080171774763 1106 3 0.0921675455110531 1106 4 0.16415283461241614 1106 5 0.10687510893416174 1106 6 0.1972061344579572 1106 7 0.20591086575709028 1106 8 0.114251651082661 1106 9 0.18903127048860568 1106 10-11 -0.3292461441259178 1106 12-13 -0.18244311044614392 1106 14-15 -0.0017576464177793127 1106 16-17 0.06935269114363507 1106 18-19 0.13426377088260466 1106 20-21 0.02764924451923889 1106 22-23 -0.10365150793663247 1106 24-25 0.048552862304127586 1106 26-27 -0.017616449963774983 1106 28-29 -0.08105831092126436 1106 30-31 0.0028747949466705336 1106 32-33 0.008388387565801736 1106 34-35 -0.19317973733173943 1106 36-37 -0.21587851702189198 1106 38-39 -0.013457628781630149 1106 40-41 0.25344771032614943 1106 42-43 0.2948663667515774 1106 44-45 0.29024409333207046 1106 46-47 0.46380817450239675 1106 48-49 0.5746932683151442 1106 50-51 0.4375270077801616 1106 52-53 0.34862507864176706 1106 54-55 0.045131645401873755 1106 56-57 0.1713252058080812 1106 58-59 0.25774355047465747 1106 60-61 0.044676677657058406 1106 62-63 0.17425869718654496 1106 64-65 0.17255619514897802 1106 66-67 0.019188747002587547 1106 68-69 -0.30735321204860355 1106 70-71 -0.0022897332521836233 1106 72-73 0.33746604870850305 1106 74-75 0.4477983533574559 1106 76-77 0.3552371123893394 1106 78-79 0.5459716086352877 1106 80-81 0.2549225014624028 1106 82-83 0.3483158118248362 1106 84-85 0.22127994551562935 1106 86-87 0.21392349844801828 1106 88-89 0.0702810197001682 1106 90-91 0.18725851449271502 1106 92-93 0.34663932318459345 1106 94-95 0.506534471042027 1106 96-97 0.5135955348221266 1106 98-99 0.4683211622574639 1106 100-101 0.3590975913617527 1107 1 0.2723083464256284 1107 2 0.14705094384877526 1107 3 0.0809620365723589 1107 4 0.03192640046918882 1107 5 0.14386757509856807 1107 6 0.04451881244601452 1107 7 -0.03891450355324366 1107 8 0.10482959437412376 1107 9 0.13476908158077094 1107 10-11 0.051849262234505034 1107 12-13 -0.04607092372893362 1107 14-15 0.16953020134350538 1107 16-17 0.1487165534963637 1107 18-19 0.07846290796793198 1107 20-21 0.1251143136190791 1107 22-23 0.2990401000753309 1107 24-25 0.2360153598039645 1107 26-27 0.34255708541477503 1107 28-29 0.21026444392905574 1107 30-31 0.2341321168633712 1107 32-33 0.29544847728606527 1107 34-35 0.560419074017581 1107 36-37 0.37668955894507405 1107 38-39 0.39710331337641946 1107 40-41 0.2625566509221926 1107 42-43 0.26872510019095586 1107 44-45 0.2588060450812293 1107 46-47 0.268080840299163 1107 48-49 0.12203547208111587 1107 50-51 0.1383715878950298 1107 52-53 0.2081209497950809 1107 54-55 0.37528746531368284 1107 56-57 0.31291535943736903 1107 58-59 0.43204564680488744 1107 60-61 0.2220346920008751 1107 62-63 0.27662528353040017 1107 64-65 0.32061439902923894 1107 66-67 0.2950804731176646 1107 68-69 0.30244482210604673 1107 70-71 0.3861937846966299 1107 72-73 0.5126146300424956 1107 74-75 0.3659228949935631 1107 76-77 0.4004713184792692 1107 78-79 0.3071621165739167 1107 80-81 0.48223456036156875 1107 82-83 0.3386049263372328 1107 84-85 0.49223586638654027 1107 86-87 0.8203846434767357 1107 88-89 0.6128131408892017 1107 90-91 0.7070691209140989 1107 92-93 0.7327893522341462 1107 94-95 0.8727324556695741 1107 96-97 0.9817872237918692 1107 98-99 0.8820194564294574 1107 100-101 0.9522726125536423 1108 1 0.31264796906713244 1108 2 0.3281776284848945 1108 3 0.33482007790210133 1108 4 0.3259785118797893 1108 5 0.20793046818032934 1108 6 0.3432932958242674 1108 7 0.3026811837782901 1108 8 0.3118700256409639 1108 9 0.29298651194914527 1108 10-11 0.358515928901177 1108 12-13 0.2175616009790673 1108 14-15 0.29909983296973763 1108 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-1.1174554140773623 2316 68-69 -1.1214362995267564 2316 70-71 -1.1309628295196177 2316 72-73 -1.1835577934574317 2316 74-75 -1.2643171651803904 2316 76-77 -0.905679750531597 2316 78-79 -1.0002647734345764 2316 80-81 -0.9533412087254263 2316 82-83 -0.963151522007152 2316 84-85 -0.9465415626298359 2316 86-87 -0.8444809791254251 2316 88-89 -0.7162005495932959 2316 90-91 -0.6417659546287311 2316 92-93 -0.8116574212100112 2316 94-95 -1.1609358626530657 2316 96-97 -0.9057055685005366 2316 98-99 -0.7799089780337525 2316 100-101 -0.7875066310646055 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 3964.0 3 1114.0 4 1087.0 5 1207.0 6 1509.0 7 1764.0 8 2306.0 9 2794.0 10 3432.0 11 4367.0 12 5499.0 13 6974.0 14 7989.0 15 10024.0 16 12297.0 17 16651.0 18 14964.0 19 11169.0 20 10605.0 21 11197.0 22 11772.0 23 12799.0 24 14135.0 25 16023.0 26 18341.0 27 20926.0 28 24337.0 29 28907.0 30 34571.0 31 42414.0 32 52265.0 33 68183.0 34 93388.0 35 139261.0 36 203319.0 37 279362.0 38 213673.0 39 24953.0 40 5.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.47390750208964 18.44341323742686 14.362561045822945 22.72011821466056 2 15.771306191865959 19.65600744675639 40.501256290199535 24.071430071178114 3 19.412070617599525 24.220047609415214 29.79760049107271 26.57028128191255 4 12.845473253909368 16.422491635284402 36.34941411991992 34.38262099088631 5 13.617537846329618 37.222698276682856 33.94156291479745 15.218200962190073 6 32.262166782095605 35.486660779674054 17.132338987133817 15.118833451096528 7 28.62399142033498 33.16751767945823 20.08744873972169 18.1210421604851 8 26.841894181768044 34.819801880407326 19.159423069250867 19.178880868573764 9 26.869016606170725 16.625628348215848 20.015384120060528 36.489970925552896 10-11 25.440781093745464 26.53885289632145 26.396216620923884 21.6241493890092 12-13 26.303228505951576 24.047948766879145 27.30296064552612 22.34586208164316 14-15 23.026272274003535 25.259088346387635 26.039791798414164 25.674847581194665 16-17 22.097175369024708 28.568358771426038 26.82795715859112 22.506508700958133 18-19 21.899696995361488 26.48483821904107 29.850051550685208 21.765413234912238 20-21 22.626955172485005 25.708503370525378 30.1588079411321 21.505733515857518 22-23 22.471238461086177 25.71014892443464 30.074777201318177 21.743835413161012 24-25 22.175989425035304 25.90288732103462 30.17134607454146 21.749777179388623 26-27 21.7496955953993 26.228354374203988 30.02852075988628 21.993429270510433 28-29 21.72425270448057 26.372714854352314 30.163205064533393 21.739827376633723 30-31 21.877854740312696 26.053606991729346 30.093381727561958 21.975156540396 32-33 21.63142089125961 26.12460974141431 30.10160723535918 22.1423621319669 34-35 22.2678520864375 26.24486051507028 29.800104793515825 21.687182604976396 36-37 22.313384019479273 26.001201879748326 29.664835147527764 22.020578953244637 38-39 21.90301801585866 26.21787337497039 29.798055965437207 22.081052643733745 40-41 22.21576025301059 26.083108855720553 29.787006962830148 21.914123928438705 42-43 22.128160329706898 25.99563092170896 29.611545340122213 22.26466340846193 44-45 22.030718896627384 26.388346634313308 29.57501934624389 22.005915122815413 46-47 22.26985822438843 26.656564910894748 28.95304083024257 22.120536034474252 48-49 22.331640569650258 26.642747560128683 28.799455609998443 22.226156260222616 50-51 22.30930972955953 26.87978868558234 28.64604835041808 22.164853234440052 52-53 22.49152997779112 26.772407249583317 28.60799855579299 22.12806421683257 54-55 22.22164690027614 26.851037136370774 28.641120273113103 22.28619569023998 56-57 22.222198898441142 27.217907905817174 28.471619448254966 22.088273747486717 58-59 22.306430435864094 26.704219261815474 28.54291102931684 22.446439273003588 60-61 22.20329429842653 26.84889254152335 28.809559546163666 22.138253613886448 62-63 22.134308950256763 26.890301518384298 28.81246416388461 22.16292536747433 64-65 22.00980824317838 26.8446606017607 28.712620853816734 22.43291030124419 66-67 21.918668391509463 27.071298088796752 28.50055516336915 22.50947835632463 68-69 22.01676827028498 26.756291949537065 28.698223610054807 22.528716170123147 70-71 21.997022951407065 26.83567167646886 28.536172470068554 22.63113290205552 72-73 22.09484068015562 26.901780375249345 28.334883955279107 22.668494989315928 74-75 21.96184300343685 27.175340317598266 28.24117371478434 22.621642964180538 76-77 22.081090678192908 27.04158438010009 28.231828589720493 22.64549635198651 78-79 22.17838247784614 27.044046058201022 28.142678836539282 22.634892627413556 80-81 22.06781761147759 27.31119202932646 28.02920611954401 22.59178423965194 82-83 22.18603295726429 27.240067883442272 28.017697833320334 22.556201325973113 84-85 22.172336686089142 27.135947531330633 27.950628012022904 22.741087770557318 86-87 22.29986193454641 27.04599744857001 27.921018305303395 22.733122311580185 88-89 22.286718021480844 27.085179826410698 27.830308671242292 22.79779348086616 90-91 22.210779049124806 27.18082558119596 27.85320823530523 22.755187134373998 92-93 22.323987717697378 26.837237097704207 27.894689331638965 22.944085852959454 94-95 22.1987254343766 26.798772816778833 27.98214306335979 23.020358685484783 96-97 22.37022081400781 26.629773937263696 27.82434955132119 23.1756556974073 98-99 22.262039343374383 26.656198052739843 27.596518905959865 23.485243697925906 100-101 22.6363586359214 26.088106581910125 27.902951799272184 23.37258298289629 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1596.0 1 1080.5 2 836.0 3 1121.0 4 820.0 5 644.0 6 1308.0 7 2823.0 8 4471.0 9 5117.5 10 3945.5 11 2563.5 12 2350.5 13 2390.5 14 2445.0 15 2523.0 16 2607.5 17 2674.5 18 2751.0 19 2878.5 20 3189.0 21 3711.0 22 4344.5 23 5077.0 24 6039.0 25 7351.0 26 8903.0 27 10567.0 28 12080.5 29 13583.5 30 15497.5 31 18124.5 32 21056.5 33 22933.5 34 24872.5 35 27100.5 36 29913.5 37 32703.5 38 34462.0 39 35950.0 40 37248.5 41 39864.5 42 42030.0 43 44023.0 44 46984.5 45 49684.0 46 50905.0 47 51870.5 48 53501.0 49 53300.0 50 52738.5 51 51526.0 52 50139.0 53 50053.5 54 49581.5 55 46914.0 56 42696.5 57 39034.0 58 35953.0 59 32005.5 60 26648.0 61 21995.5 62 18389.0 63 14392.0 64 10907.0 65 8613.0 66 6610.0 67 5059.0 68 3707.5 69 2596.5 70 1913.0 71 1338.0 72 997.0 73 793.0 74 600.0 75 474.0 76 329.5 77 187.0 78 123.5 79 77.0 80 40.0 81 22.0 82 15.0 83 10.5 84 8.0 85 10.5 86 7.5 87 4.5 88 3.5 89 2.5 90 2.0 91 1.5 92 1.0 93 2.0 94 1.5 95 0.5 96 1.5 97 1.0 98 0.5 99 0.5 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4931632188378557 2 0.05155479323170207 3 0.058619968423563554 4 0.06484571685995633 5 0.0796755895399032 6 0.10772643361848194 7 0.07365969779237759 8 0.05694111491262617 9 0.05603173592753508 10-11 0.07614300194397246 12-13 0.1094402632442305 14-15 0.058445087849507575 16-17 0.06173284264175995 18-19 0.05987910855676658 20-21 0.07418433951454552 22-23 0.06316686334901896 24-25 0.08869942716119163 26-27 0.06565016750061384 28-29 0.06613983310797056 30-31 0.07009213408163566 32-33 0.0704418952297476 34-35 0.07159610701851706 36-37 0.06655954648570492 38-39 0.04277578841409202 40-41 0.047147802765491444 42-43 0.04487435530276374 44-45 0.023014283545766595 46-47 0.029554817015460144 48-49 0.02969472147470492 50-51 0.027771035160089177 52-53 0.027211417323110047 54-55 0.02766610681565559 56-57 0.027316345667543634 58-59 0.024588208712270392 60-61 0.03088390937828557 62-63 0.020810788312661286 64-65 0.023853710301235287 66-67 0.016648630650129028 68-69 0.02098566888671726 70-71 0.04302062121777038 72-73 0.03850870240712617 74-75 0.03340218964469164 76-77 0.024098543104913654 78-79 0.015739251665037944 80-81 0.01801269912776565 82-83 0.023259116349444966 84-85 0.01923686314615749 86-87 0.010667715017414607 88-89 0.04193636165862333 90-91 0.05228929164273718 92-93 0.034906162581573044 94-95 0.05221933941311479 96-97 0.04403492854729506 98-99 0.035920469911097715 100-101 0.16792032720854927 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1429547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.35976036449211 #Duplication Level Percentage of deduplicated Percentage of total 1 81.98048288622326 51.122832673502295 2 10.877055700936035 13.565811739632078 3 2.9806822507017694 5.57623892629372 4 1.2535181920589566 3.1267637627731175 5 0.7061321555306874 2.2017116002277968 6 0.43668031675265834 1.633876794515177 7 0.302372568340269 1.3199116631746635 8 0.2339278074578929 1.1670145612532201 9 0.17846249184255006 1.0015990402816395 >10 0.9615081020881137 10.964536493143592 >50 0.06242186608809612 2.6861517819301644 >100 0.024507335165438047 2.9351631975417267 >500 0.0014614124293062056 0.626716892487626 >1k 4.4966536286344787E-4 0.42832664576055873 >5k 3.372490221475859E-4 1.643344227482654 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9712 0.6793760540926601 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8580 0.6001901301601137 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5150 0.360253982555313 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2089 0.14613020768117452 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1638 0.11458175212147624 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 1.3990445924478174E-4 0.0 0.0 0.0 0.0 3 1.3990445924478174E-4 0.0 0.0 0.0 0.0 4 1.3990445924478174E-4 0.0 0.0 0.0 0.0 5 1.3990445924478174E-4 0.0 0.0 0.0 0.0 6 1.3990445924478174E-4 0.0 0.0 0.0 0.0 7 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 8 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 9 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 10-11 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 12-13 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 14-15 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 16-17 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 18-19 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 20-21 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 22-23 1.3990445924478174E-4 0.0 0.0 6.995222962239087E-5 0.0 24-25 2.098566888671726E-4 0.0 0.0 3.147850333007589E-4 0.0 26-27 2.098566888671726E-4 0.0 0.0 0.0010143073295246676 0.0 28-29 2.798089184895635E-4 0.0 0.0 0.003847372629231498 0.0 30-31 2.798089184895635E-4 0.0 0.0 0.013430828087499048 0.0 32-33 2.798089184895635E-4 0.0 0.0 0.03095386160790796 0.0 34-35 3.147850333007589E-4 0.0 0.0 0.055786903123856715 0.0 36-37 3.4976114811195437E-4 0.0 0.0 0.09922723771936146 0.0 38-39 3.4976114811195437E-4 0.0 0.0 0.16407295457931778 0.0 40-41 3.4976114811195437E-4 0.0 0.0 0.23077240552426748 0.0 42-43 3.4976114811195437E-4 0.0 0.0 0.29390429275847524 0.0 44-45 5.246417221679315E-4 0.0 0.0 0.37323012115026644 0.0 46-47 5.59617836979127E-4 0.0 0.0 0.46927453242180917 0.0 48-49 6.295700666015178E-4 0.0 0.0 0.5696210058151288 0.0 50-51 6.295700666015178E-4 0.0 0.0 0.6766129410225756 0.0 52-53 6.295700666015178E-4 0.0 0.0 0.7749657758716573 0.0 54-55 6.295700666015178E-4 0.0 0.0 0.8802788575681667 0.0 56-57 6.995222962239087E-4 0.0 0.0 1.0012612387000916 0.0 58-59 6.995222962239087E-4 0.0 0.0 1.1339955944085784 0.0 60-61 6.995222962239087E-4 0.0 0.0 1.2793913036787177 0.0 62-63 6.995222962239087E-4 0.0 0.0 1.4184213600532196 0.0 64-65 6.995222962239087E-4 0.0 0.0 1.559340126627526 0.0 66-67 7.694745258462996E-4 0.0 0.0 1.7292890684951248 0.0 68-69 7.694745258462996E-4 0.0 0.0 1.9157817126684187 0.0 70-71 7.694745258462996E-4 0.0 0.0 2.108220296359616 0.0 72-73 7.694745258462996E-4 0.0 0.0 2.29793074309554 0.0 74-75 7.694745258462996E-4 0.0 0.0 2.491523538575507 0.0 76-77 7.694745258462996E-4 0.0 0.0 2.698722042717028 0.0 78-79 7.694745258462996E-4 0.0 0.0 2.9316979434744015 0.0 80-81 9.793312147134722E-4 0.0 0.0 3.1810076898486024 0.0 82-83 9.793312147134722E-4 0.0 0.0 3.4351441400667486 0.0 84-85 9.793312147134722E-4 6.995222962239087E-5 0.0 3.6856780504593414 0.0 86-87 9.793312147134722E-4 6.995222962239087E-5 0.0 3.9509369051874472 0.0 88-89 9.793312147134722E-4 6.995222962239087E-5 0.0 4.233438984517473 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3400 0.0 62.497387 1 GTATCAA 6285 0.0 44.521835 1 GTGGTAT 1325 0.0 37.840263 1 TGGTATC 1330 0.0 35.728123 2 TATCAAC 8190 0.0 33.883698 2 ATCAACG 8250 0.0 33.522076 3 TCAACGC 8355 0.0 32.87329 4 CAACGCA 8490 0.0 32.350574 5 AACGCAG 8780 0.0 31.336166 6 ACGCAGA 9945 0.0 27.630108 7 CGCAGAG 10070 0.0 27.192715 8 GCAGAGT 10570 0.0 25.321707 9 GTACATG 6175 0.0 24.977346 1 TACATGG 6280 0.0 24.36453 2 ACATGGG 6360 0.0 23.310913 3 GAGTACT 6915 0.0 23.301882 12-13 CAGAGTA 10335 0.0 22.53836 10-11 GTACTTT 7370 0.0 21.65844 14-15 AGAGTAC 10305 0.0 20.620361 10-11 AGTACTT 7205 0.0 20.483828 12-13 >>END_MODULE