FastQCFastQC Report
Thu 26 May 2016
SRR938117_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938117_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1429547
Sequences flagged as poor quality0
Sequence length101
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94010.6576209106800966No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85300.5966925186789941No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52920.37018719916169246No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21910.1532653351026584No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17210.1203877871801347No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA37950.058.8384931
GTATCAA64750.040.575221
TGGTATC15600.035.936262
GTGGTAT15400.035.785971
ATCAACG85150.030.792063
TATCAAC85650.030.6187322
TCAACGC86650.030.2569034
CAACGCA88050.029.829765
AACGCAG90400.029.0543166
GTACCTG13150.026.7350791
TACCTGG12900.026.5166282
ACGCAGA101850.025.741397
CGCAGAG102650.025.4019648
GTACATG64800.024.8542231
GTACCGT1201.7121606E-423.747836
TACATGG64600.023.6073912
TAAGGTG5650.023.5376725
GCAGAGT110550.023.2430179
ACATGGG64750.023.1809733
GAGTACT70650.022.72262412-13
Produced by FastQC (version 0.11.5)