##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938117_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1429547 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.49225873650884 34.0 31.0 34.0 31.0 34.0 2 32.70763605533781 34.0 31.0 34.0 31.0 34.0 3 32.759348241086165 34.0 31.0 34.0 31.0 34.0 4 36.22714678146294 37.0 35.0 37.0 35.0 37.0 5 36.19291146076345 37.0 35.0 37.0 35.0 37.0 6 36.23301787209515 37.0 37.0 37.0 35.0 37.0 7 36.1974394685869 37.0 37.0 37.0 35.0 37.0 8 36.19183349690496 37.0 37.0 37.0 35.0 37.0 9 37.894810034227625 39.0 38.0 39.0 35.0 39.0 10-11 37.88428222366946 39.0 38.0 39.0 35.0 39.0 12-13 37.823579427608884 39.0 38.0 39.0 35.0 39.0 14-15 39.12691782781538 40.0 38.5 41.0 36.0 41.0 16-17 39.07172446935988 40.0 38.0 41.0 36.0 41.0 18-19 39.108993968019234 40.0 38.5 41.0 36.0 41.0 20-21 39.048023254919215 40.0 39.0 41.0 35.5 41.0 22-23 38.901607292380035 40.0 38.0 41.0 35.0 41.0 24-25 38.8623679389345 40.0 38.0 41.0 35.0 41.0 26-27 38.823315357942064 40.0 38.0 41.0 35.0 41.0 28-29 38.76053008400564 40.0 38.0 41.0 35.0 41.0 30-31 38.54698446430932 40.0 38.0 41.0 34.5 41.0 32-33 38.349722324624516 40.0 38.0 41.0 34.0 41.0 34-35 38.146790206967665 40.0 38.0 41.0 34.0 41.0 36-37 38.01266380188969 40.0 37.0 41.0 33.5 41.0 38-39 38.000219650001014 40.0 37.5 41.0 33.0 41.0 40-41 37.931060328901395 40.0 37.5 41.0 33.0 41.0 42-43 37.76697583220419 40.0 37.0 41.0 33.0 41.0 44-45 37.53332943932588 40.0 37.0 41.0 33.0 41.0 46-47 37.16393409940352 40.0 37.0 41.0 32.0 41.0 48-49 36.79603433815048 40.0 36.0 41.0 31.0 41.0 50-51 36.48722672287095 40.0 36.0 41.0 30.5 41.0 52-53 36.07384751952891 39.0 35.0 41.0 29.5 41.0 54-55 35.85509325681492 39.0 35.0 41.0 29.0 41.0 56-57 35.620989726116036 39.0 35.0 40.0 28.0 41.0 58-59 35.28795975228516 38.0 35.0 40.0 28.0 41.0 60-61 34.94348034727085 38.0 34.0 40.0 27.0 41.0 62-63 34.58612518511109 37.5 34.0 40.0 26.0 41.0 64-65 34.19801027878062 37.0 34.0 40.0 26.0 41.0 66-67 33.7817007765397 36.0 33.5 39.0 25.5 41.0 68-69 33.3324318822676 36.0 33.0 39.0 24.5 40.0 70-71 32.82332095412043 35.0 32.5 38.5 23.5 40.0 72-73 32.41191615245948 35.0 32.0 37.5 22.0 40.0 74-75 31.96698464618512 35.0 32.0 37.0 21.5 39.0 76-77 30.666872442808806 34.0 30.0 35.5 20.0 38.5 78-79 31.394510638684842 35.0 31.5 36.0 20.5 38.5 80-81 31.23507866477982 35.0 31.5 36.0 20.0 37.5 82-83 30.872680646386584 34.5 31.0 35.0 18.5 37.0 84-85 30.49416983142212 34.0 31.0 35.0 17.0 36.5 86-87 30.05756648784545 34.0 30.5 35.0 9.5 36.0 88-89 29.605582747541703 34.0 30.0 35.0 6.5 36.0 90-91 29.26653863076905 34.0 30.0 35.0 2.0 35.0 92-93 28.851604039601355 34.0 29.0 35.0 2.0 35.0 94-95 28.44241462505255 34.0 29.0 35.0 2.0 35.0 96-97 27.86827225687578 34.0 28.5 35.0 2.0 35.0 98-99 27.1823147472591 33.0 27.0 35.0 2.0 35.0 100-101 24.778654007178496 31.0 21.5 34.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.1777024407926433 1101 2 0.18054017476431028 1101 3 0.21615386435696848 1101 4 0.1017530317485722 1101 5 0.11040330935158948 1101 6 0.16341995422466482 1101 7 0.14818892220105795 1101 8 0.1792340417518048 1101 9 0.039577310240616725 1101 10-11 0.1381993370644281 1101 12-13 0.2315860112235555 1101 14-15 0.15704563085399315 1101 16-17 0.27190333124002564 1101 18-19 0.18793102913216586 1101 20-21 0.2623986385396222 1101 22-23 0.3597432913129026 1101 24-25 0.2848097268932577 1101 26-27 0.23873956037901678 1101 28-29 0.22056876643980416 1101 30-31 0.301496820001276 1101 32-33 0.21076972846159236 1101 34-35 0.25148644733603476 1101 36-37 0.28109750453436533 1101 38-39 0.40271068232524243 1101 40-41 0.33271183252898595 1101 42-43 0.3714202504973798 1101 44-45 0.45410494735274654 1101 46-47 0.602445275475219 1101 48-49 0.7451244115174873 1101 50-51 0.6363246448642244 1101 52-53 0.576398059056352 1101 54-55 0.6509262416760109 1101 56-57 0.6188909935520641 1101 58-59 0.7081585894973088 1101 60-61 0.6960019071841899 1101 62-63 0.5502042149529913 1101 64-65 0.5184372613710053 1101 66-67 0.5120523995966053 1101 68-69 0.6576786779182058 1101 70-71 0.6964777845638181 1101 72-73 0.5751931888598065 1101 74-75 0.7489123117708019 1101 76-77 0.5995160391107142 1101 78-79 0.6048240373182914 1101 80-81 0.6905831714850841 1101 82-83 0.7034498767816579 1101 84-85 0.7191617115788205 1101 86-87 0.648735450542155 1101 88-89 0.6999917057663225 1101 90-91 0.7789751217315448 1101 92-93 0.9141611061142108 1101 94-95 0.6608578673688008 1101 96-97 0.7239451471003271 1101 98-99 0.6846593917371386 1101 100-101 0.7673642959500562 1102 1 0.1367887092969866 1102 2 0.12431007680373796 1102 3 0.02747402284404643 1102 4 0.11292734322915976 1102 5 0.12417126676426449 1102 6 0.1262204598276213 1102 7 0.11425396996595083 1102 8 0.1115634615237937 1102 9 0.2019840289260486 1102 10-11 0.13346234783280408 1102 12-13 0.10748661648862878 1102 14-15 0.2105265127422271 1102 16-17 0.13346474498366945 1102 18-19 0.19456463714403327 1102 20-21 0.19679665502033572 1102 22-23 0.15196539199595094 1102 24-25 0.13871356510031063 1102 26-27 0.08652997823069342 1102 28-29 0.1442366971138611 1102 30-31 0.1483787325416941 1102 32-33 0.07690891512145726 1102 34-35 0.1277686856014313 1102 36-37 0.06678565932666203 1102 38-39 0.18935048883488292 1102 40-41 0.20155951963854335 1102 42-43 0.15352482280103175 1102 44-45 0.1502071861042964 1102 46-47 0.012207301776300028 1102 48-49 0.09011760532385438 1102 50-51 0.18237948333866427 1102 52-53 0.15624929510973118 1102 54-55 0.28121441105299994 1102 56-57 0.3375278101980683 1102 58-59 0.12112317298257125 1102 60-61 0.16804911299862368 1102 62-63 0.18576414591874624 1102 64-65 0.1839815137835572 1102 66-67 0.17750914380525984 1102 68-69 0.24539532149243115 1102 70-71 0.3597662699426607 1102 72-73 0.4023912247867827 1102 74-75 0.4969840928058318 1102 76-77 0.37606627125053294 1102 78-79 0.3369304327095257 1102 80-81 0.46854568880441505 1102 82-83 0.5020509609110704 1102 84-85 0.40477633517200573 1102 86-87 0.2537088334634703 1102 88-89 0.2120520378113433 1102 90-91 0.11930424980952381 1102 92-93 0.02176338132750999 1102 94-95 0.10371136405078474 1102 96-97 0.10027235485938846 1102 98-99 0.012576939562801925 1102 100-101 0.4481738683752532 1103 1 0.09620677974108816 1103 2 0.13349843821261942 1103 3 0.11706054658064602 1103 4 0.025637909844782314 1103 5 0.081292246856151 1103 6 0.039696723227315545 1103 7 0.05759240794451159 1103 8 0.11998612614860349 1103 9 0.08253533061058249 1103 10-11 0.09020047953264765 1103 12-13 0.1419429717719325 1103 14-15 0.28901043310975894 1103 16-17 0.2674616821965401 1103 18-19 0.18575912412718054 1103 20-21 0.3246680179606045 1103 22-23 0.2384891285962638 1103 24-25 0.26275644412022103 1103 26-27 0.16424820181415356 1103 28-29 0.054267324985225684 1103 30-31 -0.08937011891313063 1103 32-33 0.03134995646907868 1103 34-35 -0.004314009651245954 1103 36-37 -0.00863547390458308 1103 38-39 0.030851254531668815 1103 40-41 0.09168203112398743 1103 42-43 0.14127367425585646 1103 44-45 -0.0653364586123999 1103 46-47 -0.1225553322206423 1103 48-49 -0.13844288472209598 1103 50-51 -0.11356232370575015 1103 52-53 -0.18295438023483257 1103 54-55 -0.2459678247816086 1103 56-57 -0.07977693712199141 1103 58-59 -0.17635002762998653 1103 60-61 -0.3078314383030545 1103 62-63 -0.023271076133326574 1103 64-65 -0.07903533154568976 1103 66-67 -0.16016313797115345 1103 68-69 -0.23469656212166257 1103 70-71 -0.19957523082304363 1103 72-73 -0.48237140921015254 1103 74-75 -0.26832984287564443 1103 76-77 -0.2081603749975507 1103 78-79 -0.218582584135806 1103 80-81 -0.31208218255852316 1103 82-83 -0.188224689931193 1103 84-85 -0.10824051015724834 1103 86-87 -0.2642850409622568 1103 88-89 -0.2956049300293948 1103 90-91 -0.4147692570817476 1103 92-93 -0.35342804286850793 1103 94-95 -0.2458292178787751 1103 96-97 -0.14015643533969424 1103 98-99 0.00338857815391691 1103 100-101 0.2460299173798468 1104 1 0.10841689891478268 1104 2 0.014721180016998403 1104 3 -0.06658581128640151 1104 4 -0.07531097753846439 1104 5 -0.003916886503724015 1104 6 -0.03417610568131124 1104 7 0.05845236139280985 1104 8 0.037517089462802744 1104 9 0.04766036484792835 1104 10-11 1.6817721937201213E-4 1104 12-13 0.04348301477515548 1104 14-15 0.037627909625989275 1104 16-17 -0.01656746861745262 1104 18-19 -0.04262366438414489 1104 20-21 0.11786224324290373 1104 22-23 0.04330104219506126 1104 24-25 0.1072526311609252 1104 26-27 -0.03145801901479217 1104 28-29 0.03433869391357547 1104 30-31 -0.1547044029450646 1104 32-33 -0.16775227652674118 1104 34-35 0.06417537760280823 1104 36-37 0.10800548471895866 1104 38-39 0.05378334648624872 1104 40-41 0.15290395234094944 1104 42-43 0.1344284226293837 1104 44-45 0.24649314086331486 1104 46-47 0.027280051287547735 1104 48-49 0.15299511712534297 1104 50-51 0.2817196312440018 1104 52-53 0.21679839007941837 1104 54-55 0.20751595697318237 1104 56-57 0.23938971198705872 1104 58-59 0.16031426229020695 1104 60-61 0.17581697103256744 1104 62-63 0.23671042738245518 1104 64-65 0.30068068975852924 1104 66-67 0.2856013002092297 1104 68-69 0.45000796541403787 1104 70-71 0.3171106937738486 1104 72-73 0.32470987219256386 1104 74-75 0.18931925176137554 1104 76-77 0.13568433215808184 1104 78-79 0.3462424366838306 1104 80-81 0.41840807407465164 1104 82-83 0.39323413709452026 1104 84-85 0.5668451247944581 1104 86-87 0.4982147788411808 1104 88-89 0.33156532966435037 1104 90-91 0.39738885459133044 1104 92-93 0.485170726472127 1104 94-95 0.5441791321527738 1104 96-97 0.6224667498036816 1104 98-99 0.8677812381102505 1104 100-101 0.7746229536974916 1105 1 0.06660307504211715 1105 2 0.061987538769813 1105 3 -0.0022964192095926705 1105 4 -0.03161721557516728 1105 5 -0.04425288716507225 1105 6 -0.05309234484577274 1105 7 -0.02193591420284946 1105 8 -0.032229430176784035 1105 9 -0.07759944065706748 1105 10-11 -0.06025111429299557 1105 12-13 0.020947231398729116 1105 14-15 0.03428647786228112 1105 16-17 0.1557341644894592 1105 18-19 -0.05507544759523597 1105 20-21 0.0959232534595813 1105 22-23 0.09673027074174456 1105 24-25 0.09049112559791439 1105 26-27 0.02108124969745262 1105 28-29 -0.011233475246157809 1105 30-31 0.05434506994254917 1105 32-33 0.032486900178760436 1105 34-35 0.12855866452453313 1105 36-37 0.2694586911513639 1105 38-39 0.15134570363468214 1105 40-41 0.3168816033266637 1105 42-43 0.3212340026367144 1105 44-45 0.26867758401840547 1105 46-47 0.30828104177609816 1105 48-49 0.5131594126920191 1105 50-51 0.4985817675864581 1105 52-53 0.42002536198597795 1105 54-55 0.38604067504625306 1105 56-57 0.3565248067672684 1105 58-59 0.4346997485966071 1105 60-61 0.5861341263861775 1105 62-63 0.5618254102951212 1105 64-65 0.6818932498269916 1105 66-67 0.6642378756192855 1105 68-69 0.7655119944724547 1105 70-71 0.6163081479988293 1105 72-73 0.5940059006957767 1105 74-75 0.41253944690956246 1105 76-77 0.5691710610913212 1105 78-79 0.664561780195573 1105 80-81 0.7626107563033706 1105 82-83 0.8411189260786145 1105 84-85 0.9569381470296712 1105 86-87 0.7794864824559795 1105 88-89 0.7496880042998306 1105 90-91 0.6433346639980648 1105 92-93 0.7513251049061154 1105 94-95 0.4669184734294056 1105 96-97 0.7084236530904597 1105 98-99 0.8773990809884502 1105 100-101 0.6644187273068134 1106 1 -0.1982103978410379 1106 2 -0.04180824634736524 1106 3 -0.12874489608949347 1106 4 -0.04518173744768461 1106 5 -0.05866954947391889 1106 6 -0.03277444815348218 1106 7 0.03300298917059763 1106 8 -0.17098189857014745 1106 9 -0.11204364438387415 1106 10-11 -0.1307038543652581 1106 12-13 -0.225779797290663 1106 14-15 -0.1987903666781179 1106 16-17 -0.13723206614625383 1106 18-19 -0.020636473251592236 1106 20-21 -0.017779722557470734 1106 22-23 0.06459073087766143 1106 24-25 -0.04600523749061125 1106 26-27 -0.11042752695061608 1106 28-29 -0.027102162944906638 1106 30-31 -0.033004554164413946 1106 32-33 -0.06286592132246938 1106 34-35 -0.09252471425462971 1106 36-37 -0.07919050937555738 1106 38-39 -0.1022517384499011 1106 40-41 -0.011736818545109884 1106 42-43 -0.22656056727142015 1106 44-45 -0.05517487765146001 1106 46-47 -0.11861591696406748 1106 48-49 -0.08342878832546319 1106 50-51 -0.10565751902606735 1106 52-53 -0.2823853836493555 1106 54-55 -0.6160082470782626 1106 56-57 -0.38016952582647434 1106 58-59 -0.2939166811291969 1106 60-61 -0.2712508832134688 1106 62-63 -0.15885406088973042 1106 64-65 -0.3395651504299977 1106 66-67 -0.4411292146274164 1106 68-69 -0.6669567475122662 1106 70-71 -0.6795402198110807 1106 72-73 -0.5224454896976027 1106 74-75 -0.6629686125659546 1106 76-77 -0.4866329316672271 1106 78-79 -0.5574467013658762 1106 80-81 -0.6055383159791603 1106 82-83 -0.5550334930091338 1106 84-85 -0.5191242270604768 1106 86-87 -0.3456529055069737 1106 88-89 -0.2697598226424489 1106 90-91 -0.401385526650305 1106 92-93 -0.42074550670760047 1106 94-95 -0.5131115083673592 1106 96-97 -0.35305720861227385 1106 98-99 -0.37988432916700887 1106 100-101 -0.3476183166473952 1107 1 0.11566290799839862 1107 2 0.12071687437497047 1107 3 0.05836710185322147 1107 4 0.05254668254222139 1107 5 0.06844463909103382 1107 6 0.13276706413781625 1107 7 0.08722755652270564 1107 8 -0.007842927400638189 1107 9 -0.006843773916315854 1107 10-11 0.04535716875056295 1107 12-13 0.15474414240055268 1107 14-15 0.2581312852415323 1107 16-17 0.13115011171322521 1107 18-19 0.1752101546188385 1107 20-21 0.09429441571840869 1107 22-23 0.08637551653003328 1107 24-25 0.06509574066983959 1107 26-27 0.09790398086419572 1107 28-29 0.04200482514001891 1107 30-31 0.06781907217258976 1107 32-33 -0.04137163122693721 1107 34-35 0.1167904305645493 1107 36-37 0.16914033172813703 1107 38-39 0.2206052615405767 1107 40-41 0.15055400084285253 1107 42-43 0.16555771680465625 1107 44-45 0.1475236355827505 1107 46-47 0.21896968398075956 1107 48-49 0.12901180295204995 1107 50-51 0.1309787329145351 1107 52-53 0.2977592450401758 1107 54-55 0.31738326473681155 1107 56-57 0.26302097258770374 1107 58-59 0.2359962654481933 1107 60-61 0.23412709436598078 1107 62-63 0.24410208856784976 1107 64-65 0.2829101574476738 1107 66-67 0.28993499917731214 1107 68-69 0.29243459286279716 1107 70-71 0.24942028231763658 1107 72-73 0.19063943024944763 1107 74-75 0.330388801737854 1107 76-77 0.2441326908028394 1107 78-79 0.4258033724485699 1107 80-81 0.4576092271903853 1107 82-83 0.3021396042449709 1107 84-85 0.45766874961416093 1107 86-87 0.5913455134352361 1107 88-89 0.6283460034602442 1107 90-91 0.5904605964245668 1107 92-93 0.7773012678392774 1107 94-95 0.7222684693454404 1107 96-97 0.6836935747732831 1107 98-99 0.8270523237527065 1107 100-101 1.0724556406640424 1108 1 0.087702440792647 1108 2 0.07507985730399014 1108 3 0.029518943722045776 1108 4 0.10016573016127239 1108 5 0.10630807125635044 1108 6 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scores pass #Quality Count 2 3.0 3 4.0 4 11.0 5 28.0 6 52.0 7 145.0 8 320.0 9 582.0 10 1000.0 11 1776.0 12 2581.0 13 3827.0 14 5294.0 15 7077.0 16 9309.0 17 13655.0 18 13515.0 19 8968.0 20 7254.0 21 7360.0 22 7614.0 23 8059.0 24 9193.0 25 10759.0 26 12810.0 27 15474.0 28 19246.0 29 23244.0 30 28602.0 31 35104.0 32 44393.0 33 58178.0 34 81705.0 35 126522.0 36 202168.0 37 333715.0 38 296326.0 39 33668.0 40 6.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.79415297293478 18.802040086824707 14.767964956731047 22.63584198350946 2 15.958497747180477 19.937942518120504 40.00797582066997 24.09558391402905 3 19.48579539690968 24.143802673931095 30.03845586110837 26.331946068050854 4 13.08946120694178 16.78328869215213 36.043585835233124 34.08366426567297 5 13.60584856601427 37.288175904674695 33.99349584168971 15.112479687621324 6 32.45706507026352 35.570778715215376 16.891714648066834 15.080441566454269 7 28.684961040105712 33.12629805106093 19.88343160455725 18.30530930427611 8 26.825025077962035 35.00806552028552 19.03238939464528 19.134520007107174 9 26.680713123503892 16.507384882710685 19.987030811266827 36.8248711825186 10-11 25.46481507778338 26.54711597450101 26.326626546731234 21.661442400984367 12-13 26.18014657790195 24.041217252738107 27.3371564558563 22.441479713503647 14-15 22.961959278009047 25.233203245503645 26.00575566945333 25.799081807033975 16-17 21.97551176302085 28.630713231181844 26.80306685315977 22.590708152637536 18-19 21.89756615207475 26.507662916993986 29.838298426004883 21.756472504926386 20-21 22.5637911869984 25.619164672445187 30.244790832340595 21.57225330821582 22-23 22.345120517198804 25.69471308043737 30.135910186933344 21.82425621543048 24-25 22.104594892853456 25.900518899520275 30.179384980770813 21.815501226855456 26-27 21.761051349712805 26.219688178787663 30.07734484241194 21.941915629087593 28-29 21.70949958273495 26.30200336190416 30.185646222194862 21.802850833166033 30-31 21.880183023013583 26.031358185495122 30.126291755360263 21.962167036131024 32-33 21.69009375030604 26.060899225066493 30.136085543481762 22.112921481145708 34-35 22.259711973760204 26.26071387716678 29.744287244027497 21.735286905045513 36-37 22.27879997033924 25.949039453010776 29.72693910729501 22.045221469354974 38-39 21.90949348999738 26.117600882389674 29.837567397478697 22.135338230134252 40-41 22.170869346811013 25.99767302910603 29.900491560357818 21.93096606372514 42-43 22.06173962713331 25.967573980972247 29.65048863592243 22.320197755972014 44-45 21.991362795804307 26.349593760394708 29.64393130949795 22.015112134303035 46-47 22.269292300288132 26.61895691432321 28.941475866131018 22.170274919257636 48-49 22.327317674759904 26.62077567229339 28.81035041170385 22.241556241242854 50-51 22.313537085524295 26.824511541068603 28.667263126011242 22.194688247395852 52-53 22.509333376237368 26.81240980534393 28.52228713011884 22.15596968829986 54-55 22.23634480013599 26.796810458138136 28.600633627295917 22.36621111442996 56-57 22.208154051598164 27.153916590360446 28.432818228431806 22.20511112960959 58-59 22.29643376538162 26.753824813035177 28.4687386983429 22.481002723240298 60-61 22.171219274357544 26.85469592815067 28.74435048305512 22.229734314436673 62-63 22.083254345607383 26.906775363104536 28.785482394073085 22.224487897214992 64-65 21.96807799953412 26.823637138198325 28.61007717829494 22.598207683972614 66-67 21.89704151035258 27.074310953050162 28.444290394089876 22.584357142507383 68-69 22.02035478520728 26.829101089090834 28.59246429448333 22.558079831218553 70-71 21.98602074643226 26.932482807490764 28.430369900395018 22.651126545681954 72-73 22.10738786482711 26.97399945577165 28.20445917482951 22.714153504571726 74-75 21.973639201789098 27.266329823363627 28.06661830635859 22.693412668488687 76-77 22.040793342226593 27.053290308048634 28.24457677851795 22.661339571206824 78-79 22.030860125620215 27.09907404233649 28.16762932593332 22.702436506109976 80-81 21.98416701234727 27.319353613417398 28.10921921419862 22.58726016003671 82-83 22.05134429089525 27.31637433016298 28.03859688271552 22.59368449622625 84-85 22.053682918212946 27.206550519235677 27.943763750389124 22.796002812162254 86-87 22.08553144025947 27.107970405957982 28.0086069373846 22.79789121639795 88-89 22.05249850389512 27.19395779987416 27.843989575707806 22.90955412052292 90-91 21.977462743364317 27.239805140660238 27.936006167001505 22.84672594897394 92-93 22.029600379142277 26.983982637801958 27.976083257405776 23.01033372564999 94-95 21.89994452788191 26.9319581657686 28.04210004987594 23.125997256473553 96-97 22.032329122442285 26.765646739841365 27.935108114668495 23.26691602304786 98-99 21.842303890673058 26.848470179714273 27.736338854196468 23.572887075416197 100-101 21.88917188452006 26.709824860602694 27.99393793978092 23.40706531509632 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1551.0 1 1010.0 2 776.5 3 1094.0 4 800.5 5 613.0 6 1274.0 7 2807.0 8 4474.5 9 5067.5 10 3945.0 11 2585.0 12 2249.0 13 2283.5 14 2415.5 15 2496.0 16 2571.0 17 2663.0 18 2741.0 19 2895.0 20 3183.0 21 3633.5 22 4168.5 23 4912.0 24 5914.5 25 7115.0 26 8687.0 27 10459.5 28 11854.0 29 13298.0 30 15420.0 31 17832.5 32 20914.0 33 23350.0 34 25050.5 35 27244.0 36 29971.0 37 32592.5 38 34421.0 39 35817.0 40 37034.0 41 39648.5 42 42372.5 43 44599.5 44 47485.5 45 50150.5 46 51371.5 47 52473.0 48 53795.0 49 53481.0 50 52872.0 51 51758.0 52 50074.5 53 50088.5 54 50598.5 55 47822.0 56 43204.0 57 39429.5 58 36297.5 59 32198.5 60 26633.5 61 21897.5 62 18211.5 63 13953.5 64 10291.5 65 7979.0 66 5986.0 67 4717.0 68 3486.5 69 2346.5 70 1704.0 71 1190.5 72 860.0 73 667.0 74 517.5 75 410.0 76 264.5 77 143.0 78 73.5 79 31.5 80 22.5 81 14.5 82 6.5 83 3.0 84 3.0 85 2.0 86 1.0 87 0.5 88 0.0 89 1.0 90 1.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01587915612428273 3 0.008114458636197341 4 0.0 5 0.0 6 0.0 7 0.0 8 3.497611481119543E-4 9 3.497611481119543E-4 10-11 0.0 12-13 0.0 14-15 0.0 16-17 2.4483280367836805E-4 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.005561202254980074 26-27 0.020880740542283675 28-29 0.0 30-31 0.0 32-33 3.497611481119543E-4 34-35 0.01871222142398956 36-37 0.0034276592514971525 38-39 0.01724322460191935 40-41 0.015459442746548382 42-43 0.0075898169140294095 44-45 0.0023433996923500943 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 0.0 68-69 4.1971337773434527E-4 70-71 0.0 72-73 0.0 74-75 0.0 76-77 0.0 78-79 0.0 80-81 0.0 82-83 1.0492834443358632E-4 84-85 0.0029379936441404166 86-87 1.7488057405597716E-4 88-89 1.0492834443358632E-4 90-91 2.0985668886717263E-4 92-93 3.147850333007589E-4 94-95 0.0 96-97 0.0 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1429547.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.58310743046249 #Duplication Level Percentage of deduplicated Percentage of total 1 80.69721450263873 48.888880155524376 2 11.500299613683579 13.93447773956597 3 3.2557960441175053 5.917387245373368 4 1.4053906438605732 3.405717294350878 5 0.7812788682432934 2.3666150803966794 6 0.48065008537121573 1.74715654551032 7 0.3180155998675193 1.3486461275935828 8 0.239355972879346 1.1600742895257827 9 0.1909454939133339 1.0411264234002806 >10 1.0334401673861986 11.487566062915512 >50 0.06647355700038075 2.7425587629510657 >100 0.02871754768584909 3.2236956835206207 >500 0.0016146022350236886 0.6819984252006104 >1k 4.613149242924825E-4 0.4315154465770894 >5k 3.4598619321936185E-4 1.622584717593871 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9401 0.6576209106800966 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8530 0.5966925186789941 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5292 0.37018719916169246 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2191 0.1532653351026584 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1721 0.1203877871801347 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 6.995222962239087E-5 0.0 0.0 0.0 0.0 7 6.995222962239087E-5 0.0 0.0 0.0 0.0 8 6.995222962239087E-5 0.0 0.0 0.0 0.0 9 6.995222962239087E-5 0.0 0.0 0.0 0.0 10-11 6.995222962239087E-5 0.0 0.0 0.0 0.0 12-13 6.995222962239087E-5 0.0 0.0 0.0 0.0 14-15 6.995222962239087E-5 0.0 0.0 0.0 0.0 16-17 6.995222962239087E-5 0.0 0.0 0.0 0.0 18-19 6.995222962239087E-5 0.0 0.0 0.0 0.0 20-21 6.995222962239087E-5 0.0 0.0 0.0 0.0 22-23 6.995222962239087E-5 0.0 0.0 0.0 0.0 24-25 2.798089184895635E-4 0.0 0.0 2.4483280367836805E-4 0.0 26-27 2.798089184895635E-4 0.0 0.0 8.74402870279886E-4 0.0 28-29 2.798089184895635E-4 0.0 0.0 0.0037074681699867163 0.0 30-31 2.798089184895635E-4 0.0 0.0 0.013360875857876656 0.0 32-33 2.798089184895635E-4 0.0 0.0 0.03102381383753035 0.0 34-35 4.1971337773434527E-4 0.0 0.0 0.056346520960835846 0.0 36-37 4.896656073567361E-4 0.0 0.0 0.10010164058964133 0.0 38-39 5.59617836979127E-4 0.0 0.0 0.16526214248289844 0.0 40-41 6.295700666015178E-4 0.0 0.0 0.23220642623152649 0.0 42-43 6.645461814127132E-4 0.0 0.0 0.2957930029582798 0.0 44-45 7.694745258462996E-4 0.0 0.0 0.3746291657427143 0.0 46-47 8.394267554686904E-4 0.0 0.0 0.4715829559993481 0.0 48-49 8.394267554686904E-4 0.0 0.0 0.5722442144259685 0.0 50-51 8.394267554686904E-4 0.0 0.0 0.6795159585519049 0.0 52-53 8.394267554686904E-4 0.0 0.0 0.7784284112379656 0.0 54-55 8.394267554686904E-4 0.0 0.0 0.8845459435751326 0.0 56-57 8.394267554686904E-4 0.0 0.0 1.0060879425440366 0.0 58-59 8.394267554686904E-4 0.0 0.0 1.1399065578116705 0.0 60-61 8.394267554686904E-4 0.0 0.0 1.2853722193114323 0.0 62-63 8.394267554686904E-4 0.0 0.0 1.4247170607192348 6.995222962239087E-5 64-65 8.394267554686904E-4 0.0 0.0 1.5666151585082546 6.995222962239087E-5 66-67 9.093789850910813E-4 0.0 0.0 1.7371586943276438 6.995222962239087E-5 68-69 9.093789850910813E-4 0.0 0.0 1.9245607174860289 6.995222962239087E-5 70-71 0.0010143073295246676 0.0 0.0 2.118188489080807 6.995222962239087E-5 72-73 0.0010842595591470585 0.0 0.0 2.308843290916633 6.995222962239087E-5 74-75 0.001119235673958254 0.0 0.0 2.503170584807635 6.995222962239087E-5 76-77 0.0011891879035806447 0.0 0.0 2.713132202019241 6.995222962239087E-5 78-79 0.0012591401332030356 0.0 0.0 2.949535762028111 6.995222962239087E-5 80-81 0.0016788535109373808 0.0 0.0 3.201853454276075 6.995222962239087E-5 82-83 0.0016788535109373808 0.0 0.0 3.459872253238264 6.995222962239087E-5 84-85 0.001783781855370967 0.0 0.0 3.7133091811601857 6.995222962239087E-5 86-87 0.0018887101998045534 0.0 0.0 3.982695217436013 6.995222962239087E-5 88-89 0.0018887101998045534 0.0 0.0 4.2707235229062075 6.995222962239087E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3795 0.0 58.838493 1 GTATCAA 6475 0.0 40.57522 1 TGGTATC 1560 0.0 35.93626 2 GTGGTAT 1540 0.0 35.78597 1 ATCAACG 8515 0.0 30.79206 3 TATCAAC 8565 0.0 30.618732 2 TCAACGC 8665 0.0 30.256903 4 CAACGCA 8805 0.0 29.82976 5 AACGCAG 9040 0.0 29.054316 6 GTACCTG 1315 0.0 26.735079 1 TACCTGG 1290 0.0 26.516628 2 ACGCAGA 10185 0.0 25.74139 7 CGCAGAG 10265 0.0 25.401964 8 GTACATG 6480 0.0 24.854223 1 GTACCGT 120 1.7121606E-4 23.74783 6 TACATGG 6460 0.0 23.607391 2 TAAGGTG 565 0.0 23.537672 5 GCAGAGT 11055 0.0 23.243017 9 ACATGGG 6475 0.0 23.180973 3 GAGTACT 7065 0.0 22.722624 12-13 >>END_MODULE