FastQCFastQC Report
Thu 26 May 2016
SRR938116_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938116_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452131
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93230.6420219663377478No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85280.5872748395289406No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50780.3496929684718527No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20810.14330663004921732No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15920.10963198223851706No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA34250.061.5748181
GTATCAA65250.044.5050541
GTGGTAT12800.036.0762861
TATCAAC83050.034.6485942
TCAACGC83850.034.431474
ATCAACG84450.034.1305163
CAACGCA86000.033.6259885
AACGCAG87450.033.28146
TGGTATC14250.031.7087172
ACGCAGA100100.028.9734867
CGCAGAG101550.028.4631968
ACCGTAT700.00835781527.1657688
GCAGAGT108000.026.189269
GTACATG64600.025.8663181
TACATGG64850.024.7166632
GAGTACT71000.023.60020612-13
CAGAGTA103200.023.399910-11
ACATGGG65200.023.197943
TACCTGG12600.022.2715992
GTACTTT75100.022.05631314-15
Produced by FastQC (version 0.11.5)