##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938116_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1452131 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.96029077266445 33.0 31.0 34.0 30.0 34.0 2 32.07518949736628 33.0 31.0 34.0 30.0 34.0 3 32.087971402029154 34.0 31.0 34.0 30.0 34.0 4 35.610387079402614 37.0 35.0 37.0 33.0 37.0 5 35.616595196989806 37.0 35.0 37.0 33.0 37.0 6 35.645299218872125 37.0 35.0 37.0 33.0 37.0 7 35.577460298003416 37.0 35.0 37.0 33.0 37.0 8 35.57522014198444 37.0 35.0 37.0 33.0 37.0 9 37.206510982824554 39.0 37.0 39.0 34.0 39.0 10-11 37.1159079311715 39.0 37.0 39.0 33.5 39.0 12-13 36.96156958290953 39.0 37.0 39.0 33.0 39.0 14-15 38.12497460628552 40.0 38.0 41.0 33.0 41.0 16-17 37.94737871445483 40.0 38.0 41.0 33.0 41.0 18-19 38.13476194640842 40.0 38.0 41.0 33.0 41.0 20-21 38.142876228108896 40.0 38.0 41.0 33.0 41.0 22-23 38.025810343557154 40.0 38.0 41.0 33.0 41.0 24-25 37.9021954630815 40.0 38.0 41.0 33.0 41.0 26-27 37.717325434137834 40.0 38.0 41.0 32.0 41.0 28-29 37.532429581077736 40.0 37.0 41.0 32.0 41.0 30-31 37.291996038924864 40.0 37.0 41.0 31.5 41.0 32-33 36.97444273278376 40.0 36.0 41.0 31.0 41.0 34-35 36.72637971367597 39.0 36.0 41.0 30.0 41.0 36-37 36.45358235586183 39.0 35.0 41.0 30.0 41.0 38-39 36.156153267163916 39.0 35.0 40.5 30.0 41.0 40-41 36.02146707149699 39.0 35.0 41.0 29.5 41.0 42-43 35.78013381712807 39.0 35.0 40.5 28.0 41.0 44-45 35.417858994815205 39.0 35.0 40.0 26.5 41.0 46-47 34.98191347750306 38.0 34.5 40.0 24.5 41.0 48-49 34.542567440540836 38.0 34.0 40.0 23.5 41.0 50-51 33.806647265294934 37.5 33.5 39.5 22.5 40.5 52-53 33.87165792893341 38.0 33.5 40.0 22.0 40.5 54-55 33.949647793484196 38.0 33.5 40.0 21.5 41.0 56-57 33.63646668241364 38.0 33.0 40.0 19.5 41.0 58-59 33.22539770860894 37.5 33.0 40.0 16.5 41.0 60-61 32.72747052435352 37.0 32.0 40.0 11.0 41.0 62-63 32.18104633810586 36.0 31.0 39.0 8.0 41.0 64-65 31.643728768272283 35.5 31.0 39.0 4.5 40.0 66-67 31.079527604603165 35.0 30.5 38.5 2.0 40.0 68-69 30.399405425543563 35.0 29.0 38.0 2.0 40.0 70-71 29.815144088239972 34.5 29.0 37.5 2.0 39.5 72-73 29.124373765176834 34.0 28.0 36.5 2.0 39.0 74-75 28.448843458338125 34.0 26.0 36.0 2.0 39.0 76-77 27.701798598060368 33.5 26.0 35.5 2.0 38.0 78-79 26.950642882770218 33.0 24.5 35.0 2.0 37.0 80-81 26.22677224024554 32.0 22.5 35.0 2.0 36.5 82-83 25.48580878722374 32.0 19.5 35.0 2.0 36.0 84-85 24.749742275318134 31.0 16.5 34.5 2.0 35.5 86-87 23.998483607883863 31.0 7.5 34.0 2.0 35.0 88-89 23.125441850631933 30.5 2.0 34.0 2.0 35.0 90-91 22.229500644225624 29.5 2.0 34.0 2.0 35.0 92-93 21.295780132784166 29.0 2.0 34.0 2.0 35.0 94-95 20.210291977789883 28.0 2.0 34.0 2.0 35.0 96-97 18.95156015538543 25.5 2.0 33.0 2.0 35.0 98-99 17.413460975628233 21.5 2.0 33.0 2.0 34.5 100-101 13.559024289130939 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06497832218310151 1101 2 -0.04604164262561028 1101 3 0.011232404189406964 1101 4 -0.21001646084379644 1101 5 -0.21911192383088007 1101 6 -0.2705344393291611 1101 7 -0.06258787847755087 1101 8 -0.012903343822500801 1101 9 -0.11287477095185494 1101 10-11 -0.061096230239250815 1101 12-13 -0.06702911060356342 1101 14-15 -0.07615890808929038 1101 16-17 -0.019845995617139067 1101 18-19 0.16808454817345364 1101 20-21 0.0036914344016238942 1101 22-23 -0.011422379902072066 1101 24-25 -0.1518974032092899 1101 26-27 -0.06392004824336084 1101 28-29 -0.08312548098938777 1101 30-31 -0.1716918653439521 1101 32-33 -0.008414616177141454 1101 34-35 0.08304971772179925 1101 36-37 -0.18568650644569829 1101 38-39 -9.06413254789129E-4 1101 40-41 0.20127405985827096 1101 42-43 0.14669983554653498 1101 44-45 0.21194013453628457 1101 46-47 0.18661223767782786 1101 48-49 0.41251982340726556 1101 50-51 0.35428291229870723 1101 52-53 0.16878374232455684 1101 54-55 0.0707726053868285 1101 56-57 -9.047713439827021E-4 1101 58-59 -0.03738456362221143 1101 60-61 0.12401436608497107 1101 62-63 -0.18242505368593243 1101 64-65 -0.48423985741690245 1101 66-67 -0.48430941599294997 1101 68-69 -0.668638204818901 1101 70-71 -0.7117967270104195 1101 72-73 -0.6890846045357328 1101 74-75 -0.7503775580794212 1101 76-77 -0.9987028239265001 1101 78-79 -1.2628000326822928 1101 80-81 -1.195921818867813 1101 82-83 -1.1511533015536415 1101 84-85 -1.2907239899772769 1101 86-87 -1.3622712341576637 1101 88-89 -1.0612967192418452 1101 90-91 -1.182384479806629 1101 92-93 -1.6948422905957052 1101 94-95 -1.958725717316998 1101 96-97 -1.755456776337514 1101 98-99 -1.8335560259321912 1101 100-101 -1.7615940023583363 1102 1 0.13235011705489796 1102 2 0.1126442548102844 1102 3 0.15120035290735245 1102 4 -0.004920306997647117 1102 5 0.046336794117834756 1102 6 0.14702966323494593 1102 7 0.1002326343429587 1102 8 -0.029954625873784835 1102 9 0.09363163930455443 1102 10-11 0.10872748770946572 1102 12-13 0.1013522996528522 1102 14-15 0.19917763037225455 1102 16-17 0.15084310694695802 1102 18-19 0.1403601891990931 1102 20-21 -0.053183565598374116 1102 22-23 0.29385005599536385 1102 24-25 0.08502567371378689 1102 26-27 -0.06150017644849015 1102 28-29 -0.22509663483553766 1102 30-31 0.0787248013227142 1102 32-33 -0.11591461617714316 1102 34-35 -0.03315220535512964 1102 36-37 -0.005253814138008295 1102 38-39 -0.10440000299838204 1102 40-41 0.1038541880633943 1102 42-43 0.30944022016192463 1102 44-45 0.3394080832542343 1102 46-47 0.4288077504983434 1102 48-49 0.43325700289444313 1102 50-51 0.4984335533243467 1102 52-53 0.3095689987348109 1102 54-55 0.2893783746175984 1102 56-57 0.10390292096370501 1102 58-59 0.03104492355727473 1102 60-61 0.3105368019824013 1102 62-63 -0.143466720352599 1102 64-65 -0.022332806134848227 1102 66-67 -0.26969403137756487 1102 68-69 -0.35171512789582593 1102 70-71 -0.6889441629078554 1102 72-73 -0.35076729684342567 1102 74-75 -0.6298967888486509 1102 76-77 -0.7626771829008625 1102 78-79 -0.6319827249899888 1102 80-81 -0.8603609214319157 1102 82-83 -0.6662975323228757 1102 84-85 -0.6202592463875369 1102 86-87 -0.8047071315935597 1102 88-89 -1.0563608218059493 1102 90-91 -0.9382498644220121 1102 92-93 -0.8402589572623711 1102 94-95 -1.2399436660349465 1102 96-97 -1.5845593404400802 1102 98-99 -1.5318733336244996 1102 100-101 -1.5708407972301313 1103 1 0.2272454854393402 1103 2 0.22817494868310462 1103 3 0.32232608474898683 1103 4 0.27643903448980467 1103 5 0.24902881197187554 1103 6 0.27080857460239827 1103 7 0.24107084662050937 1103 8 0.3127710132196029 1103 9 0.22703607962685624 1103 10-11 0.3899775601690365 1103 12-13 0.4363007084407471 1103 14-15 0.36681631798456493 1103 16-17 0.41959622270836405 1103 18-19 0.416094929214168 1103 20-21 0.5482893924910073 1103 22-23 0.5697682636355097 1103 24-25 0.4748778561959597 1103 26-27 0.3419190456255947 1103 28-29 0.5064934179150242 1103 30-31 0.48912912570970235 1103 32-33 0.2844560869705006 1103 34-35 0.4410443629597509 1103 36-37 0.33374624382964413 1103 38-39 0.22761234411192532 1103 40-41 0.4224565879242732 1103 42-43 0.4310809946097791 1103 44-45 0.5164943391234615 1103 46-47 0.22000130449521293 1103 48-49 0.3644096896952078 1103 50-51 0.5369284231869642 1103 52-53 0.46110948914116534 1103 54-55 0.38065047478570335 1103 56-57 0.35302760938756705 1103 58-59 0.5494177715081179 1103 60-61 0.5093232491448916 1103 62-63 0.43145995056503494 1103 64-65 0.04118409143630686 1103 66-67 0.12163371773842613 1103 68-69 0.08131831944056245 1103 70-71 0.020580502328943595 1103 72-73 0.1831211733901803 1103 74-75 0.4430224622092602 1103 76-77 0.21318827417409025 1103 78-79 -0.03537933967898965 1103 80-81 0.09114275951411699 1103 82-83 -0.3540518654050153 1103 84-85 -0.04237274705416283 1103 86-87 -0.5666893355237654 1103 88-89 -0.6355834755651522 1103 90-91 -0.7246628259894585 1103 92-93 -0.7024330341274769 1103 94-95 -0.7206175400132651 1103 96-97 -0.8970041262495982 1103 98-99 -0.7651061896908153 1103 100-101 -1.1415595961403397 1104 1 0.43559673165286483 1104 2 0.24665223368312894 1104 3 0.3610801079222483 1104 4 0.21676368468634877 1104 5 0.29610248496460656 1104 6 0.24814614354061604 1104 7 0.2844322185425483 1104 8 0.30324310408070687 1104 9 0.3851369491882437 1104 10-11 0.40030556266592754 1104 12-13 0.33769353694003 1104 14-15 0.3493683928603062 1104 16-17 0.45866015476401145 1104 18-19 0.46965164281487404 1104 20-21 0.457209759119273 1104 22-23 0.5722202869331667 1104 24-25 0.5218959327462045 1104 26-27 0.3983860403296191 1104 28-29 0.33921308028498487 1104 30-31 0.45064675446088387 1104 32-33 0.5019909297013854 1104 34-35 0.45921476660643634 1104 36-37 0.45194290441006046 1104 38-39 0.37793494109332215 1104 40-41 0.4259517325933686 1104 42-43 0.5032636173637002 1104 44-45 0.5479828430452116 1104 46-47 0.17229597885143733 1104 48-49 0.39346406026366054 1104 50-51 0.2750265184308276 1104 52-53 0.5622000601496566 1104 54-55 0.24179423122264154 1104 56-57 0.16234254561955197 1104 58-59 0.3264964041438887 1104 60-61 0.24923180675849466 1104 62-63 0.1829545866092488 1104 64-65 -0.037907903061295656 1104 66-67 0.09072429755427436 1104 68-69 0.039009151477106485 1104 70-71 0.14068835942737223 1104 72-73 -0.056691328983674794 1104 74-75 0.044161198452581374 1104 76-77 -0.24288255459271468 1104 78-79 -0.3589178826008208 1104 80-81 -0.4119522008610339 1104 82-83 -0.7423019150840133 1104 84-85 -0.6124129801628904 1104 86-87 -0.6499273934354441 1104 88-89 -0.3459034413485682 1104 90-91 -0.4968985130706578 1104 92-93 -0.8256022735516311 1104 94-95 -1.1578194316134152 1104 96-97 -1.3229026005401003 1104 98-99 -1.6374248310678894 1104 100-101 -1.4872362571391058 1105 1 0.20392964863442842 1105 2 0.3398259619919912 1105 3 0.3272254389324445 1105 4 0.23173969966236285 1105 5 0.3995680073490462 1105 6 0.38709959330487465 1105 7 0.32399775810808507 1105 8 0.36079240487325137 1105 9 0.1538203394932509 1105 10-11 0.4106039445859295 1105 12-13 0.4137349170354696 1105 14-15 0.3608676070622252 1105 16-17 0.46093190703576425 1105 18-19 0.4150355138744217 1105 20-21 0.4460494236346122 1105 22-23 0.5425496896950008 1105 24-25 0.3638646125527174 1105 26-27 0.40108890114058937 1105 28-29 0.41289759315868935 1105 30-31 0.24524883284674814 1105 32-33 0.3548540595915384 1105 34-35 0.3677285805256574 1105 36-37 0.4717480728638819 1105 38-39 0.3164463728479916 1105 40-41 0.3830627572719649 1105 42-43 0.5115242472459514 1105 44-45 0.25679458564074054 1105 46-47 0.37066256235315365 1105 48-49 0.3738536284910694 1105 50-51 0.44497867486947484 1105 52-53 0.46108692525273653 1105 54-55 0.47328502352424806 1105 56-57 0.47634714340792783 1105 58-59 0.5498372423495965 1105 60-61 0.6058953323409355 1105 62-63 0.6589297720438978 1105 64-65 0.6226133588113214 1105 66-67 0.5261107194271837 1105 68-69 0.38550209932140334 1105 70-71 0.343533359569669 1105 72-73 0.12142606534993305 1105 74-75 0.13648904753718583 1105 76-77 0.4574798822562052 1105 78-79 0.5591367111294474 1105 80-81 0.32720262613162987 1105 82-83 0.12793046190511603 1105 84-85 0.02241252628423851 1105 86-87 -0.17487529676172286 1105 88-89 -0.23524083068596013 1105 90-91 -0.16679243296458068 1105 92-93 -0.2023729543763686 1105 94-95 -0.27326534435662353 1105 96-97 -0.3665678874486282 1105 98-99 -0.7966640984152669 1105 100-101 -1.002407008796343 1106 1 0.30885206447456426 1106 2 0.27608388480054913 1106 3 0.3119768865003465 1106 4 0.2847802772282577 1106 5 0.3026951193369243 1106 6 0.3131640351921021 1106 7 0.2857365291823015 1106 8 0.1403545269986637 1106 9 0.4266720482626809 1106 10-11 0.4089976921885352 1106 12-13 0.4817831662546084 1106 14-15 0.4220610286195736 1106 16-17 0.1551834185341079 1106 18-19 0.45151525249023194 1106 20-21 0.49135270509295736 1106 22-23 0.6011881767353913 1106 24-25 0.5155806883194316 1106 26-27 0.3492727057326235 1106 28-29 0.3209793145315416 1106 30-31 0.24251009830420855 1106 32-33 0.35054959998644364 1106 34-35 0.31362116368089943 1106 36-37 0.16367753931866247 1106 38-39 0.15289916934825953 1106 40-41 0.19387487939737724 1106 42-43 -0.007191960948105702 1106 44-45 -0.12682974537283798 1106 46-47 -0.02073217160486962 1106 48-49 0.12140086852248544 1106 50-51 -0.027893126325110984 1106 52-53 0.08979660340862239 1106 54-55 -0.06741812002699277 1106 56-57 -0.12535705956209853 1106 58-59 0.0393457998961253 1106 60-61 0.2588072498889247 1106 62-63 0.035143308858700095 1106 64-65 0.07137824547177374 1106 66-67 -0.3522804481253772 1106 68-69 -0.22751722137196495 1106 70-71 -0.5525140265884758 1106 72-73 -0.24274514494469202 1106 74-75 -0.2319683565214845 1106 76-77 -0.7978548849456502 1106 78-79 -0.58313535400654 1106 80-81 -0.6947706098447703 1106 82-83 -0.754746899411483 1106 84-85 -0.5854284284712747 1106 86-87 -0.751278455838932 1106 88-89 -0.9685407146978662 1106 90-91 -0.5499903707511251 1106 92-93 -0.4651481977686984 1106 94-95 -0.7130668403290059 1106 96-97 -0.5796920084050292 1106 98-99 -0.9302119412194365 1106 100-101 -1.2722185966459065 1107 1 0.14994385752842732 1107 2 -0.04660582140148506 1107 3 -0.022390745558247716 1107 4 0.04933535807704459 1107 5 0.02461542433834296 1107 6 0.043098152209644525 1107 7 0.1408890716968898 1107 8 0.021235293849819925 1107 9 0.03146925743278928 1107 10-11 0.11054590047240254 1107 12-13 0.07724346924943859 1107 14-15 0.10133035875350771 1107 16-17 0.1371920552245811 1107 18-19 0.21156230280544008 1107 20-21 0.08779905811872624 1107 22-23 0.07115571598927062 1107 24-25 0.22054950259295936 1107 26-27 0.13304661757650393 1107 28-29 0.20603942857265167 1107 30-31 0.036888097735676695 1107 32-33 0.06380238412770467 1107 34-35 0.20432010436949355 1107 36-37 0.14784952901817405 1107 38-39 0.021638509347923218 1107 40-41 0.17329428561445326 1107 42-43 0.11267922026353716 1107 44-45 -0.13414490932782286 1107 46-47 -0.10174503885233577 1107 48-49 -0.004181773652653931 1107 50-51 -0.09443556566762368 1107 52-53 -0.24375637470284772 1107 54-55 -0.14290137337548714 1107 56-57 -0.17196843357343283 1107 58-59 -0.0867327413141794 1107 60-61 -0.008473969280679228 1107 62-63 -0.167452087693313 1107 64-65 -0.15464354691322413 1107 66-67 0.018015042903503087 1107 68-69 0.054382829628817575 1107 70-71 -0.09597495246175924 1107 72-73 0.020461236842177044 1107 74-75 -0.25311800620461256 1107 76-77 -0.20626980560180286 1107 78-79 -0.162751447680769 1107 80-81 -0.10926956892708972 1107 82-83 -0.02547850986580613 1107 84-85 -0.01928678423938024 1107 86-87 -0.11584471923709216 1107 88-89 -0.12974970504951955 1107 90-91 -0.2412485434180489 1107 92-93 -0.3789709783984314 1107 94-95 -0.5124571301138019 1107 96-97 -0.6360160013485263 1107 98-99 -0.6976593309824928 1107 100-101 -0.5246076908385042 1108 1 -0.029572959868179538 1108 2 0.01597758814362038 1108 3 0.034149070856074104 1108 4 0.11610533402799916 1108 5 -0.018150385369345656 1108 6 -0.07886777266249112 1108 7 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sequence quality scores pass #Quality Count 2 4472.0 3 1464.0 4 1399.0 5 1574.0 6 1790.0 7 2390.0 8 2911.0 9 3692.0 10 4498.0 11 5760.0 12 7425.0 13 9222.0 14 10773.0 15 12968.0 16 15355.0 17 20135.0 18 19616.0 19 15675.0 20 15993.0 21 16978.0 22 18797.0 23 20659.0 24 23366.0 25 25469.0 26 28990.0 27 32948.0 28 37560.0 29 43675.0 30 51236.0 31 60337.0 32 73358.0 33 91153.0 34 116336.0 35 154869.0 36 188945.0 37 201737.0 38 102484.0 39 6121.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.586430441687476 18.408598324441105 14.337551538020731 22.667419695850686 2 15.767948187956456 19.699738183822515 40.554223508336776 23.97809011988425 3 19.39540692995082 24.222758173117043 29.801160991939817 26.58067390499232 4 12.860363016027012 16.487420349335522 36.26747039625882 34.38474623837864 5 13.607020236031838 37.19812929863879 33.89211037634824 15.302740088981132 6 32.187206734750205 35.53125862544852 17.163538504002208 15.11799613579906 7 28.667682483098663 33.18877837842312 19.9702428785359 18.17329625994232 8 26.81030585692864 34.83691397636059 19.185038480197584 19.16774168651319 9 26.888658911003382 16.56819828720867 19.985300414167035 36.55784238762091 10-11 25.41887657117489 26.532503002069397 26.377953298691775 21.670667128063936 12-13 26.278641609995816 24.055072996514056 27.31807941769689 22.348205975793235 14-15 23.07251503726345 25.25839612866967 25.988046043592117 25.681042790474763 16-17 22.019031857674804 28.569990346159152 26.80292373465729 22.608054061508756 18-19 21.918586550704212 26.54582225901308 29.810386491334707 21.725204698948 20-21 22.693122300590645 25.67288063072614 30.191494078581492 21.44250299010172 22-23 22.432599051769074 25.711285314150224 30.09375708301178 21.762358551068925 24-25 22.112634065579904 25.900804065669618 30.16840451866825 21.818157350082227 26-27 21.771315819238655 26.17631155704035 29.990323844309756 22.062048779411235 28-29 21.753385553134567 26.341499572496346 30.108944479686684 21.796170394682406 30-31 21.914040971834233 26.003779608107784 30.055639443447017 22.02653997661096 32-33 21.67295779183058 26.073148507901855 30.0558116861514 22.19808201411616 34-35 22.277736497244927 26.27979608182644 29.700064379250307 21.742403041678326 36-37 22.368810115414703 25.93036519845195 29.66609344145349 22.03473124467986 38-39 21.918315053774183 26.210879406584088 29.730014637656843 22.140790901984886 40-41 22.22967153360114 26.07528474667365 29.801211336121657 21.893832383603552 42-43 22.106132118538927 26.034931311217914 29.600070836975384 22.25886573326778 44-45 22.035255692641126 26.438256036407555 29.503322960634954 22.02316531031637 46-47 22.362391997599584 26.588304030309747 28.909522400389413 22.13978157170126 48-49 22.34531473040823 26.636689061457997 28.722008807270683 22.29598740086309 50-51 22.354153812551957 26.875253852617178 28.526540366484802 22.244051968346064 52-53 22.53230997601183 26.84511703917493 28.486610194884477 22.135962789928758 54-55 22.255335273954906 26.868284616842068 28.508879204919303 22.367500904283723 56-57 22.276811022184752 27.23126984225387 28.36248065283383 22.129438482727554 58-59 22.385467302774746 26.803281314537625 28.330188734493316 22.481062648194317 60-61 22.287085380782486 26.86795218802937 28.634240904710172 22.21072152647797 62-63 22.163302160015682 26.866618230723887 28.71211032922317 22.25796928003726 64-65 22.042266280598994 26.803939764074354 28.576998937004973 22.57679501832168 66-67 21.965804723602307 27.056277056277057 28.422007654980284 22.555910565140355 68-69 22.115352811466373 26.749965545755238 28.582345644983466 22.552335997794927 70-71 22.084952646187737 26.81788789353996 28.366881717614806 22.7302777426575 72-73 22.188469324058595 26.923765913967085 28.162158139945078 22.725606622029243 74-75 22.04674529083444 27.158338124339267 28.09364352642379 22.701273058402506 76-77 22.233583444773956 27.001393768302464 28.06220056969892 22.70282221722466 78-79 22.261533020094426 27.037192269981038 27.992613411919727 22.708661298004813 80-81 22.19124080904195 27.269950091836392 27.846489087604652 22.692320011517005 82-83 22.33110084889971 27.165066204809758 27.795619362035456 22.70821358425508 84-85 22.33995255047238 27.05373836362152 27.673667922872646 22.93264116303345 86-87 22.3655139992182 26.969112002355732 27.741027809013435 22.924346189412635 88-89 22.358162528390924 27.037462237315047 27.586330459326557 23.018044774967475 90-91 22.35982539012842 27.055050918687286 27.575681742348408 23.00944194883589 92-93 22.42478396356094 26.71715241626208 27.627715380843377 23.230348239333605 94-95 22.315239669148102 26.65970385419581 27.665670600850973 23.35938587580511 96-97 22.456200436066144 26.510519888971274 27.520852341168005 23.512427333794577 98-99 22.38440475320985 26.510699958795957 27.309252167346283 23.79564312064791 100-101 23.227313644996947 25.461170646843872 27.296130769222813 24.015384938936368 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1563.0 1 1064.5 2 817.5 3 1048.0 4 808.5 5 702.5 6 1248.5 7 2576.0 8 4101.5 9 4778.5 10 3848.0 11 2610.0 12 2340.0 13 2371.0 14 2457.5 15 2589.0 16 2710.5 17 2797.5 18 2900.0 19 3059.5 20 3361.0 21 3807.5 22 4436.5 23 5237.5 24 6246.0 25 7541.0 26 9018.0 27 10733.5 28 12425.0 29 13940.5 30 15895.5 31 18360.5 32 21156.5 33 23377.5 34 25391.0 35 27604.0 36 30146.5 37 32652.5 38 34664.5 39 36302.0 40 37753.0 41 40235.5 42 42437.0 43 44573.0 44 47413.0 45 50173.5 46 51496.5 47 52189.5 48 53284.0 49 53244.0 50 52649.5 51 51742.0 52 50507.5 53 50521.0 54 50339.5 55 47501.0 56 43650.5 57 40267.5 58 37037.5 59 32917.5 60 27803.0 61 23208.5 62 19257.0 63 15233.5 64 11726.0 65 9190.5 66 7138.0 67 5561.5 68 4176.0 69 2945.5 70 2132.5 71 1552.5 72 1178.5 73 914.0 74 701.0 75 559.5 76 390.5 77 224.5 78 140.0 79 88.5 80 51.5 81 31.0 82 21.5 83 14.0 84 8.5 85 8.5 86 9.0 87 6.0 88 5.0 89 6.0 90 2.0 91 1.0 92 1.0 93 0.5 94 1.0 95 2.0 96 1.0 97 0.5 98 0.5 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13690224917724364 2 0.05033981093992208 3 0.14902236781667771 4 0.130291275373916 5 0.19523032012952 6 0.20184129393284764 7 0.16486115922048356 8 0.06851998889907315 9 0.12051254328982715 10-11 0.10088621481119815 12-13 0.14905679997190335 14-15 0.13659235978021267 16-17 0.13297698348151785 18-19 0.13025684321869033 20-21 0.13287368701584085 22-23 0.1928545014189491 24-25 0.214856648608149 26-27 0.14977987523164232 28-29 0.12746783864541145 30-31 0.09995654662010521 32-33 0.12447224114077862 34-35 0.09317341204064922 36-37 0.08787086013589683 38-39 0.05078742895785573 40-41 0.05791488508956837 42-43 0.06276981897638712 44-45 0.03883947109455001 46-47 0.0493412784383778 48-49 0.041525179202151874 50-51 0.051476072062369024 52-53 0.025720819953571682 54-55 0.04861820317863884 56-57 0.025342066246089368 58-59 0.04823944947115653 60-61 0.03636035591830214 62-63 0.0502365144742451 64-65 0.039390385578160644 66-67 0.036291491607850807 68-69 0.06411267303018806 70-71 0.052991086892298284 72-73 0.05047753956082475 74-75 0.04314349049775812 76-77 0.021451232705589234 78-79 0.021382368395137904 80-81 0.025101041159509712 82-83 0.021382368395137904 84-85 0.01876552459798737 86-87 0.024343533744545086 88-89 0.0663163309646306 90-91 0.10901220344445507 92-93 0.07991703227876824 94-95 0.07499323408149815 96-97 0.06714270269004656 98-99 0.05626214163873645 100-101 0.12956820011417702 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1452131.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 62.475242355170714 #Duplication Level Percentage of deduplicated Percentage of total 1 82.21213566519053 51.36223100218952 2 10.598121474905824 13.24240415308561 3 3.0066275358834638 5.635193519281473 4 1.2779153636651106 3.193522882174956 5 0.6947122981413778 2.1701159596750097 6 0.46902384978330847 1.7581427211340428 7 0.31051498328208427 1.3579649184821998 8 0.21221592869345157 1.060659326140081 9 0.16794703683529458 0.9443278646206029 >10 0.9616463855611025 11.018138251308347 >50 0.061691335146815525 2.6457833780138507 >100 0.025243536490843422 2.9870076031251838 >500 0.0014329941734312528 0.6264111413097978 >1k 4.409212841326931E-4 0.4190536274043667 >5k 3.3069096309951986E-4 1.579043652055008 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9323 0.6420219663377478 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8528 0.5872748395289406 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5078 0.3496929684718527 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2081 0.14330663004921732 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1592 0.10963198223851706 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 6.88643104513298E-5 0.0 8 0.0 0.0 0.0 6.88643104513298E-5 0.0 9 0.0 0.0 0.0 6.88643104513298E-5 0.0 10-11 0.0 0.0 0.0 6.88643104513298E-5 0.0 12-13 0.0 0.0 0.0 6.88643104513298E-5 0.0 14-15 1.721607761283245E-4 0.0 0.0 2.0659293135398942E-4 0.0 16-17 2.0659293135398942E-4 0.0 0.0 2.0659293135398942E-4 0.0 18-19 2.4102508657965432E-4 0.0 0.0 2.4102508657965432E-4 0.0 20-21 2.754572418053192E-4 0.0 0.0 3.098893970309841E-4 0.0 22-23 2.754572418053192E-4 0.0 0.0 3.44321552256649E-4 0.0 24-25 3.7875370748231395E-4 0.0 0.0 6.88643104513298E-4 0.0 26-27 4.1318586270797883E-4 0.0 0.0 0.0013428540538009313 0.0 28-29 4.1318586270797883E-4 0.0 0.0 0.004304019403208113 0.0 30-31 4.1318586270797883E-4 0.0 0.0 0.012051254328982717 0.0 32-33 4.476180179336437E-4 0.0 0.0 0.02871641745820453 0.0 34-35 5.509144836106384E-4 0.0 0.0 0.05405848370429389 0.0 36-37 5.509144836106384E-4 0.0 0.0 0.09651333109753872 0.0 38-39 6.197787940619683E-4 0.0 0.0 0.16093589352475776 0.0 40-41 6.197787940619683E-4 0.0 0.0 0.2269079029371317 0.0 42-43 8.263717254159577E-4 0.0 0.0 0.2916747869166074 0.0 44-45 0.0010329646567699472 0.0 0.0 0.3758958385985838 0.0 46-47 0.0011018289672212768 0.0 0.0 0.47075642624529057 0.0 48-49 0.0011018289672212768 0.0 0.0 0.5713327516594577 0.0 50-51 0.0011018289672212768 0.0 0.0 0.6781412971694702 6.88643104513298E-5 52-53 0.0011018289672212768 0.0 0.0 0.774861221198363 6.88643104513298E-5 54-55 0.0011018289672212768 0.0 0.0 0.8807745306725082 6.88643104513298E-5 56-57 0.0011018289672212768 0.0 0.0 1.006865083108893 6.88643104513298E-5 58-59 0.0011018289672212768 0.0 0.0 1.1448002969429067 6.88643104513298E-5 60-61 0.0011018289672212768 0.0 0.0 1.29203219268785 6.88643104513298E-5 62-63 0.0011018289672212768 0.0 0.0 1.4337893757519122 6.88643104513298E-5 64-65 0.0011018289672212768 0.0 0.0 1.574582458469656 6.88643104513298E-5 66-67 0.0011018289672212768 0.0 0.0 1.7377564420840819 6.88643104513298E-5 68-69 0.0011706932776726067 0.0 0.0 1.9230014371981592 6.88643104513298E-5 70-71 0.0012395575881239366 0.0 0.0 2.1188859682769667 6.88643104513298E-5 72-73 0.0012395575881239366 0.0 0.0 2.3144606099587435 6.88643104513298E-5 74-75 0.0012395575881239366 0.0 0.0 2.509966387330069 6.88643104513298E-5 76-77 0.0012395575881239366 0.0 0.0 2.7193827554125627 6.88643104513298E-5 78-79 0.0012395575881239366 0.0 0.0 2.9521096925828316 6.88643104513298E-5 80-81 0.0015494469851549206 0.0 0.0 3.2005032603807786 6.88643104513298E-5 82-83 0.0016871756060575804 0.0 0.0 3.449826496369818 6.88643104513298E-5 84-85 0.0017216077612832452 0.0 0.0 3.6967739136482862 6.88643104513298E-5 86-87 0.0017216077612832452 0.0 0.0 3.9549117813750962 6.88643104513298E-5 88-89 0.0017216077612832452 0.0 0.0 4.2327448418909865 6.88643104513298E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3425 0.0 61.574818 1 GTATCAA 6525 0.0 44.505054 1 GTGGTAT 1280 0.0 36.076286 1 TATCAAC 8305 0.0 34.648594 2 TCAACGC 8385 0.0 34.43147 4 ATCAACG 8445 0.0 34.130516 3 CAACGCA 8600 0.0 33.625988 5 AACGCAG 8745 0.0 33.2814 6 TGGTATC 1425 0.0 31.708717 2 ACGCAGA 10010 0.0 28.973486 7 CGCAGAG 10155 0.0 28.463196 8 ACCGTAT 70 0.008357815 27.165768 8 GCAGAGT 10800 0.0 26.18926 9 GTACATG 6460 0.0 25.866318 1 TACATGG 6485 0.0 24.716663 2 GAGTACT 7100 0.0 23.600206 12-13 CAGAGTA 10320 0.0 23.3999 10-11 ACATGGG 6520 0.0 23.19794 3 TACCTGG 1260 0.0 22.271599 2 GTACTTT 7510 0.0 22.056313 14-15 >>END_MODULE