FastQCFastQC Report
Thu 26 May 2016
SRR938116_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938116_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1452131
Sequences flagged as poor quality0
Sequence length101
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT90980.6265274964861985No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85670.5899605476365424No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT51630.3555464348602158No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT22110.1522589904078902No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16230.11176677586250829No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA36050.059.3014641
GTATCAA63400.041.587411
GTGGTAT14000.037.6663131
TGGTATC14950.033.0461162
ATCAACG82300.031.9704273
TCAACGC83050.031.735624
TATCAAC83300.031.7072092
CAACGCA85250.030.9166375
AACGCAG87000.030.3493376
ACGCAGA101000.026.0493567
GGACCGA3100.026.042396
CGCAGAG101050.025.8484768
GTACCTG11850.023.6533051
TACCTGG11450.023.6481742
GCAGAGT110550.023.3694659
GTACATG65750.023.0490531
GAGTACT72000.022.23134812-13
TACATGG67150.022.2132382
ACATGGG66400.021.8157583
GTGTACG1754.0452396E-621.7175141
Produced by FastQC (version 0.11.5)