##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938116_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1452131 Sequences flagged as poor quality 0 Sequence length 101 %GC 45 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.505517752874916 34.0 31.0 34.0 31.0 34.0 2 32.721346765546635 34.0 31.0 34.0 31.0 34.0 3 32.76739977316096 34.0 31.0 34.0 31.0 34.0 4 36.23333914089018 37.0 35.0 37.0 35.0 37.0 5 36.20695584627007 37.0 35.0 37.0 35.0 37.0 6 36.245003377794426 37.0 37.0 37.0 35.0 37.0 7 36.20134891411312 37.0 37.0 37.0 35.0 37.0 8 36.18692321835977 37.0 37.0 37.0 35.0 37.0 9 37.87156737236516 39.0 38.0 39.0 35.0 39.0 10-11 37.85056100310509 39.0 38.0 39.0 35.0 39.0 12-13 37.76126912792303 39.0 38.0 39.0 35.0 39.0 14-15 39.01613112040167 40.0 38.0 41.0 35.5 41.0 16-17 38.897505803539765 40.0 38.0 41.0 35.0 41.0 18-19 39.16321495787915 40.0 38.5 41.0 36.0 41.0 20-21 39.156579881567154 40.0 39.0 41.0 36.0 41.0 22-23 39.05654861717021 40.0 39.0 41.0 35.0 41.0 24-25 38.95426239092754 40.0 38.0 41.0 35.0 41.0 26-27 38.780565940676155 40.0 38.0 41.0 35.0 41.0 28-29 38.66080918319352 40.0 38.0 41.0 34.5 41.0 30-31 38.40169585250918 40.0 38.0 41.0 34.0 41.0 32-33 38.167502449847845 40.0 37.5 41.0 34.0 41.0 34-35 38.00001790472072 40.0 37.0 41.0 33.0 41.0 36-37 37.9940718158348 40.0 37.0 41.0 33.0 41.0 38-39 37.84992951737826 40.0 37.0 41.0 33.0 41.0 40-41 37.672826005367284 40.0 37.0 41.0 33.0 41.0 42-43 37.42681307678164 40.0 37.0 41.0 33.0 41.0 44-45 37.12476284853088 40.0 36.0 41.0 31.5 41.0 46-47 36.68475399258055 39.5 36.0 41.0 30.5 41.0 48-49 36.2519869075173 39.0 35.0 41.0 30.0 41.0 50-51 35.85956260144573 39.0 35.0 40.5 28.5 41.0 52-53 35.34024134186241 38.5 35.0 40.0 27.0 41.0 54-55 35.025879896510716 38.0 34.5 40.0 26.5 41.0 56-57 34.679462114643925 38.0 34.0 40.0 26.0 41.0 58-59 34.241371474061225 37.5 33.5 40.0 24.5 41.0 60-61 33.76572051695061 37.0 33.0 40.0 23.0 41.0 62-63 33.303303903022524 36.5 32.5 39.0 22.0 41.0 64-65 32.81040828961024 36.0 32.0 39.0 20.5 40.0 66-67 32.26287056746258 35.0 31.0 39.0 18.5 40.0 68-69 31.68863415215294 35.0 31.0 38.0 16.0 40.0 70-71 31.082076617054522 35.0 30.0 37.5 10.5 39.5 72-73 30.499232851581574 34.0 29.5 37.0 7.5 39.0 74-75 29.894947838728047 34.0 29.0 36.0 6.5 39.0 76-77 28.55136210162857 32.5 27.0 35.0 2.0 37.0 78-79 29.241677231599628 34.0 29.0 35.0 2.0 37.0 80-81 28.916952740489666 34.0 29.0 35.0 2.0 37.0 82-83 28.3514545175332 34.0 27.5 35.0 2.0 36.5 84-85 27.75826767695201 33.0 26.5 35.0 2.0 36.0 86-87 27.104889297177735 33.0 25.5 35.0 2.0 35.5 88-89 26.369016293984494 32.0 24.5 35.0 2.0 35.0 90-91 25.825788100384884 32.0 23.5 34.0 2.0 35.0 92-93 25.00397829121477 31.5 19.0 34.0 2.0 35.0 94-95 24.175935917627264 31.0 12.0 34.0 2.0 35.0 96-97 23.131455770863646 31.0 2.0 34.0 2.0 35.0 98-99 21.696048428137683 30.0 2.0 34.0 2.0 35.0 100-101 17.396834376512864 23.5 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.06181043356030358 1101 2 0.029418556842607302 1101 3 0.10177399849280278 1101 4 0.06809882292861147 1101 5 0.08958847242991652 1101 6 0.06624871301092838 1101 7 -0.016751737040003434 1101 8 -0.017726793557436338 1101 9 -0.083799019790888 1101 10-11 0.0402426326171863 1101 12-13 0.13068371198000506 1101 14-15 0.07849425086494222 1101 16-17 0.15674646720525232 1101 18-19 0.14852901934214202 1101 20-21 0.19377388120260974 1101 22-23 0.04840169220211976 1101 24-25 0.03043473794402729 1101 26-27 0.03809638829610407 1101 28-29 0.10668415541677234 1101 30-31 0.2277956273704973 1101 32-33 0.1773676152910113 1101 34-35 0.04709334628687145 1101 36-37 0.08294619427368133 1101 38-39 0.10663599504973575 1101 40-41 0.0036354702348120327 1101 42-43 0.1460311837982431 1101 44-45 0.18159034142912134 1101 46-47 0.20597031810251565 1101 48-49 0.2862924399691451 1101 50-51 0.3157083987444267 1101 52-53 0.1525852756501962 1101 54-55 0.05791186769251766 1101 56-57 0.16351189040919678 1101 58-59 0.25345140887900186 1101 60-61 0.18544372616382532 1101 62-63 0.27863439876132645 1101 64-65 0.0906115235654994 1101 66-67 -0.14998606828712013 1101 68-69 -0.06433919063064408 1101 70-71 -0.17421415439235233 1101 72-73 -0.3701599366737618 1101 74-75 -0.5058378651606468 1101 76-77 -0.46617828054559496 1101 78-79 -0.4254549640750369 1101 80-81 -0.47082443149383835 1101 82-83 -0.5058362156831677 1101 84-85 -0.5304932501399975 1101 86-87 -0.667932983633996 1101 88-89 -0.6047562814722767 1101 90-91 -0.6185027986195628 1101 92-93 -0.44369517219793053 1101 94-95 -0.720360852027536 1101 96-97 -0.7208887423791488 1101 98-99 -0.5440473863572599 1101 100-101 -0.7950256863451699 1102 1 0.10386171561158619 1102 2 0.0677518901759413 1102 3 0.12395348567228837 1102 4 0.0969129254927168 1102 5 0.10029360063504811 1102 6 0.05323589249810823 1102 7 0.037607237318972864 1102 8 0.0387155141348714 1102 9 0.0716496981578274 1102 10-11 0.08295096595051632 1102 12-13 0.12166127608256971 1102 14-15 0.10951989189058509 1102 16-17 0.2093746723334604 1102 18-19 0.12756748088060021 1102 20-21 0.003036701715437573 1102 22-23 0.053049128099559084 1102 24-25 0.0383033276876219 1102 26-27 -0.006454893755183377 1102 28-29 -0.03817161381399359 1102 30-31 -0.04377488545000574 1102 32-33 -0.07953943599103752 1102 34-35 -0.0017688332003089613 1102 36-37 -0.04666919034169581 1102 38-39 -0.054405671616926554 1102 40-41 -0.03623632463698101 1102 42-43 -0.24148484184279084 1102 44-45 -0.1768070944683089 1102 46-47 -0.11318032292312807 1102 48-49 0.06948154253325356 1102 50-51 -0.04485249869146912 1102 52-53 -0.06539549358057428 1102 54-55 0.16520353435918622 1102 56-57 0.19801509553740004 1102 58-59 0.15199307554566843 1102 60-61 0.022607187702284648 1102 62-63 0.12855427055619373 1102 64-65 -0.062369245665273354 1102 66-67 -0.2857392734153308 1102 68-69 -0.36236803678448837 1102 70-71 -0.5132365902897931 1102 72-73 -0.4888778853917124 1102 74-75 -0.4654692754170533 1102 76-77 -0.4092552036225179 1102 78-79 -0.643612015357089 1102 80-81 -0.6779398161092196 1102 82-83 -0.6232721131190679 1102 84-85 -0.6708778655246128 1102 86-87 -0.8157374708134846 1102 88-89 -0.8690030763440717 1102 90-91 -0.9039675422093083 1102 92-93 -0.7172528645056246 1102 94-95 -0.8508736725403594 1102 96-97 -0.9122829731483826 1102 98-99 -1.0880057196905923 1102 100-101 -1.277589788909271 1103 1 0.11826609796622733 1103 2 0.15977090356646784 1103 3 0.10995951430831497 1103 4 0.13205465640686498 1103 5 0.09679699174280643 1103 6 0.1327895415619338 1103 7 0.12891614694739673 1103 8 0.05942271951427358 1103 9 0.15231168876707102 1103 10-11 0.14602122274722973 1103 12-13 0.19510600392442967 1103 14-15 0.1878623068230496 1103 16-17 0.19201133107787172 1103 18-19 0.2148693764228966 1103 20-21 0.25363695677311426 1103 22-23 0.19925949271610222 1103 24-25 0.1719787667925985 1103 26-27 0.18751647022371998 1103 28-29 0.1912634913972937 1103 30-31 0.16583847029748 1103 32-33 0.2217584597831177 1103 34-35 0.07358245078322767 1103 36-37 0.08644599138690268 1103 38-39 0.17180643202923562 1103 40-41 0.3154676345537908 1103 42-43 0.2609463215680279 1103 44-45 0.10834217224086018 1103 46-47 0.19051801340520313 1103 48-49 0.17054582866158796 1103 50-51 0.034969540610603644 1103 52-53 0.08977551099886938 1103 54-55 0.2405296024132113 1103 56-57 0.29568508568289786 1103 58-59 0.3780779401911971 1103 60-61 0.23176984493954933 1103 62-63 0.011269765310117918 1103 64-65 0.05424950107404669 1103 66-67 0.10030588338717195 1103 68-69 0.26134392194254374 1103 70-71 0.026200253951124353 1103 72-73 0.018747469660169713 1103 74-75 0.011905369531064025 1103 76-77 -0.08854075228636304 1103 78-79 0.0067050677105591205 1103 80-81 0.11347140642860154 1103 82-83 0.09406137479467702 1103 84-85 -0.03806527495015288 1103 86-87 -0.5190868418644392 1103 88-89 -0.8499543255470172 1103 90-91 -1.0543938943048374 1103 92-93 -1.0549260428720935 1103 94-95 -1.2456733942956184 1103 96-97 -1.2146599368026614 1103 98-99 -1.4392928914487513 1103 100-101 -1.582289226623029 1104 1 0.1431958730538483 1104 2 0.19635446364338094 1104 3 0.17377817522670824 1104 4 0.09111475726211893 1104 5 0.0030839585605377806 1104 6 0.08132264869297501 1104 7 0.08036669115950446 1104 8 0.08059560628523599 1104 9 0.19411415846012403 1104 10-11 0.20570620559765018 1104 12-13 0.12515989944605366 1104 14-15 0.3459527176477337 1104 16-17 0.16185851792540973 1104 18-19 0.2297540853645046 1104 20-21 0.25164236559137265 1104 22-23 0.1860729523396003 1104 24-25 0.2676197855175886 1104 26-27 0.21911121289200963 1104 28-29 0.12521519295118821 1104 30-31 0.13195023676159678 1104 32-33 0.11430952731738842 1104 34-35 0.10904169666427066 1104 36-37 0.1740359846877979 1104 38-39 0.19758033726097324 1104 40-41 0.2231059802458617 1104 42-43 0.22728405179097422 1104 44-45 0.08417340786353833 1104 46-47 0.06076472399488608 1104 48-49 -0.005749114319030468 1104 50-51 0.16866494806922105 1104 52-53 0.14391984046449124 1104 54-55 0.031734410349521625 1104 56-57 0.2019503093374766 1104 58-59 0.3168202936161322 1104 60-61 0.015047205682847675 1104 62-63 0.008706824357403775 1104 64-65 0.07437458756505322 1104 66-67 -0.2536408860466679 1104 68-69 -0.33982013545280054 1104 70-71 -0.5060008202161832 1104 72-73 -0.5995642370240084 1104 74-75 -0.4245674380287312 1104 76-77 -0.30885541714165043 1104 78-79 -0.22527521702028963 1104 80-81 -0.43375006502757785 1104 82-83 -0.4793523513614666 1104 84-85 -0.22776666700800874 1104 86-87 -0.3717334251878164 1104 88-89 -0.46409382197536075 1104 90-91 -0.5372897313423053 1104 92-93 -0.426580279238447 1104 94-95 -0.4927194892510407 1104 96-97 -0.8106554516559932 1104 98-99 -0.7207575879830017 1104 100-101 -1.4422461916737817 1105 1 0.0773226990725675 1105 2 0.07516447758852962 1105 3 -0.008990237271433443 1105 4 0.011173109752903088 1105 5 0.0385908589323094 1105 6 0.060553019815237974 1105 7 0.08611761582935173 1105 8 0.1240168794362404 1105 9 0.12814875465688402 1105 10-11 0.10914421714377198 1105 12-13 0.08402724344853851 1105 14-15 0.14965919702989083 1105 16-17 0.14495964291842967 1105 18-19 0.13654794486106425 1105 20-21 0.037107630786358925 1105 22-23 0.14931119936163384 1105 24-25 0.10399485837915279 1105 26-27 0.17658983928955507 1105 28-29 0.059687749045366445 1105 30-31 0.13231125063612126 1105 32-33 0.16171486526326362 1105 34-35 0.2120689956375159 1105 36-37 0.24232847865597051 1105 38-39 0.3142218120105582 1105 40-41 0.2544124710118112 1105 42-43 0.20087391051596626 1105 44-45 0.28917026150904945 1105 46-47 0.3587255489827399 1105 48-49 0.4667387297904355 1105 50-51 0.6020383881994178 1105 52-53 0.4458926627075783 1105 54-55 0.45608487524052777 1105 56-57 0.4232781658004683 1105 58-59 0.535050018602611 1105 60-61 0.5157679158630089 1105 62-63 0.405363572365502 1105 64-65 0.7336802603842401 1105 66-67 0.5571939045178524 1105 68-69 0.5078889284724788 1105 70-71 0.3911007390475376 1105 72-73 0.3806031890893635 1105 74-75 0.31996307001528734 1105 76-77 0.4650987757314624 1105 78-79 0.39005764831257395 1105 80-81 0.414103140933733 1105 82-83 0.28770357785662526 1105 84-85 0.3756145309677841 1105 86-87 0.2590410839650694 1105 88-89 0.010810443469445374 1105 90-91 -0.25878439202615766 1105 92-93 -0.26684771410047503 1105 94-95 -0.27511165677833915 1105 96-97 -0.6914199472853539 1105 98-99 -0.6378719645568367 1105 100-101 -1.3584622497817342 1106 1 0.2086986192143101 1106 2 0.17153962143202506 1106 3 0.14354209326722867 1106 4 0.08543288654432502 1106 5 0.07064170190086827 1106 6 0.1143459217094076 1106 7 0.08120752943192144 1106 8 0.0883162931027428 1106 9 0.16232642649278262 1106 10-11 0.11768806429521561 1106 12-13 0.13805246400855964 1106 14-15 0.07145514018658616 1106 16-17 0.23209740845809534 1106 18-19 0.30401830853396916 1106 20-21 0.259448580975409 1106 22-23 0.29366499956986303 1106 24-25 0.3142660832864479 1106 26-27 0.17495333409291902 1106 28-29 0.17851046992505104 1106 30-31 0.17955641932116606 1106 32-33 0.35617015505083316 1106 34-35 0.4192584306750504 1106 36-37 0.525040887555086 1106 38-39 0.275701436464864 1106 40-41 0.2689206860738196 1106 42-43 0.37932090304524024 1106 44-45 0.32343755012760056 1106 46-47 0.15764489616158528 1106 48-49 0.3119791082586616 1106 50-51 0.03078162983823063 1106 52-53 0.2146288491652939 1106 54-55 0.08507938635529655 1106 56-57 0.08819644899406853 1106 58-59 0.009836308262315185 1106 60-61 -0.059337797692066374 1106 62-63 0.004357581169635694 1106 64-65 -0.13291574030986197 1106 66-67 -0.154575690163135 1106 68-69 -0.27248488196462617 1106 70-71 -0.7674113712050783 1106 72-73 -0.8501583138240356 1106 74-75 -0.374726821668272 1106 76-77 -0.49505486282407674 1106 78-79 -0.22322253142329984 1106 80-81 -0.4294721919612208 1106 82-83 -0.4358001072768083 1106 84-85 -0.7977157428371733 1106 86-87 -0.5632642478339314 1106 88-89 -0.5648392496644234 1106 90-91 -0.6808760134848661 1106 92-93 -0.8148611897247129 1106 94-95 -0.6475253278470738 1106 96-97 -0.8464820968095275 1106 98-99 -0.9857820098561234 1106 100-101 -2.017477812278308 1107 1 0.007539187413229342 1107 2 0.08855465412269581 1107 3 0.03495744868418171 1107 4 -0.022797469792315894 1107 5 -0.0017407454679911893 1107 6 0.028907064127011495 1107 7 0.03486056523279757 1107 8 0.044865295661139726 1107 9 0.10812973067484677 1107 10-11 0.07797433755909111 1107 12-13 0.09128275763931981 1107 14-15 0.04246725496344084 1107 16-17 0.03892095828000919 1107 18-19 0.1433783740825163 1107 20-21 0.0917019796343439 1107 22-23 0.14216862815037246 1107 24-25 0.09189899885924291 1107 26-27 0.08819504019461988 1107 28-29 0.10745738730377496 1107 30-31 -0.014698572067217697 1107 32-33 -0.02715248344556187 1107 34-35 0.02694115562196231 1107 36-37 -0.011924753800698795 1107 38-39 0.10221306631621019 1107 40-41 -0.21357653396530196 1107 42-43 -0.02039044086727415 1107 44-45 -0.06909344748121526 1107 46-47 -0.18005868894284305 1107 48-49 -0.30628209937306394 1107 50-51 -0.23767741485852412 1107 52-53 -0.18400081824270131 1107 54-55 -0.0870116876710938 1107 56-57 -0.20244166698673638 1107 58-59 -0.10958704842547462 1107 60-61 -0.1411094061398046 1107 62-63 -0.16511761238253087 1107 64-65 -0.5900452948472648 1107 66-67 -0.46224420177626513 1107 68-69 -0.21437951935739719 1107 70-71 -0.29930253887225433 1107 72-73 -0.4419471872919267 1107 74-75 -0.43823220601353796 1107 76-77 -0.6251941325931512 1107 78-79 -0.5279814900039774 1107 80-81 -0.5027508109268233 1107 82-83 -0.31855003202974785 1107 84-85 -0.532665285502258 1107 86-87 -0.7688601209506665 1107 88-89 -0.9116806497614078 1107 90-91 -1.1496443027011267 1107 92-93 -1.1808947708159572 1107 94-95 -1.2232484312030927 1107 96-97 -1.5188783394729377 1107 98-99 -1.43992676429988 1107 100-101 -1.5568215742969365 1108 1 0.03616940791927448 1108 2 -0.015453238029188299 1108 3 -0.017456770737965144 1108 4 0.05896420754400111 1108 5 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-0.4859606043106055 2316 44-45 -0.269935503212146 2316 46-47 -0.29227190632554567 2316 48-49 0.03148861090446786 2316 50-51 0.2117333034813953 2316 52-53 0.27222809383597735 2316 54-55 0.3284545488013535 2316 56-57 0.2857346731804 2316 58-59 0.2515659190379651 2316 60-61 0.38288839543385933 2316 62-63 0.5813238067488697 2316 64-65 0.6614847619266797 2316 66-67 0.6310278193897645 2316 68-69 0.9696575751532102 2316 70-71 1.2228306465006398 2316 72-73 1.4046648084430977 2316 74-75 1.3554264879234061 2316 76-77 1.3869839429371034 2316 78-79 1.4031299890933688 2316 80-81 1.44129815238853 2316 82-83 1.567058180523759 2316 84-85 1.7638858676225588 2316 86-87 2.129346520634659 2316 88-89 2.05039087627166 2316 90-91 1.9582496138620762 2316 92-93 1.7653685413148743 2316 94-95 2.302487730662225 2316 96-97 2.7257664618182424 2316 98-99 2.948523142825227 2316 100-101 3.455774232208576 >>END_MODULE >>Per sequence quality scores pass #Quality Count 3 5.0 4 17.0 5 33.0 6 46.0 7 135.0 8 421.0 9 640.0 10 1189.0 11 1975.0 12 3017.0 13 4732.0 14 6242.0 15 8701.0 16 11774.0 17 16612.0 18 17032.0 19 12818.0 20 11465.0 21 12206.0 22 13223.0 23 14253.0 24 15993.0 25 18135.0 26 20817.0 27 24422.0 28 29403.0 29 35315.0 30 42636.0 31 51951.0 32 64147.0 33 82759.0 34 112585.0 35 159657.0 36 221957.0 37 275794.0 38 152528.0 39 7496.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.81642987849182 18.795854823791444 14.74599535399769 22.641719943719046 2 15.98227734589962 19.986404275146064 39.92218617051684 24.109132208437476 3 19.424615513879793 24.142305491104356 30.05772026641818 26.37535872859767 4 13.142005192372583 16.805726760000827 35.97308780894273 34.07918023868387 5 13.646658921673883 37.22726962835742 34.025263734909146 15.100807715059558 6 32.38725849714724 35.554109379142695 16.9449178950558 15.113714228654265 7 28.659948723634436 33.10982273637847 19.937113111695844 18.293115428291248 8 26.861970442060667 35.06735962526797 18.933002601005004 19.137667331666357 9 26.643326256377698 16.531222045393974 19.91645381856045 36.90899787966788 10-11 25.482032957081696 26.61275050253731 26.25083411896034 21.654382421420657 12-13 26.198529257325564 24.03500804167139 27.310487021248537 22.455975679754513 14-15 22.900757576279275 25.254160953798245 26.005814902374507 25.83926656754797 16-17 22.010906337513855 28.620269452716997 26.816078738839806 22.552745470929345 18-19 21.897129115761594 26.52908036533894 29.7727615483727 21.801028970526765 20-21 22.572274815426432 25.63133078214018 30.19658694704541 21.599807455387978 22-23 22.410788007418063 25.680465467647203 30.109025976306548 21.799720548628187 24-25 22.151167134902554 25.863871965864178 30.173581854737492 21.81137904449578 26-27 21.7811174317955 26.202382206940538 30.041285342050507 21.975215019213458 28-29 21.719721272208623 26.31302745267647 30.162373125550097 21.804878149564814 30-31 21.908403649254257 25.998199131933386 30.051047550836646 22.04234966797571 32-33 21.6683963085975 26.078156860503633 30.06274915968325 22.190697671215613 34-35 22.25210802829322 26.20105185334659 29.759123310917257 21.78771680744293 36-37 22.323051813899678 25.97808721042338 29.659153513483734 22.039707462193206 38-39 21.91551912688728 26.18632290516028 29.775730463359075 22.12242750459337 40-41 22.150888849405895 26.03357064448641 29.862399473315236 21.953141032792463 42-43 22.094123115859066 26.004975595478925 29.61422779274665 22.286673495915363 44-45 22.039712670551072 26.402989812902554 29.527845628252546 22.029451888293824 46-47 22.28126958330177 26.652779021162015 28.88973439017168 22.176217005364535 48-49 22.315426882470536 26.65022405157697 28.733142761519186 22.301206304433308 50-51 22.353605135273707 26.83274859519949 28.60132176413813 22.21232450538867 52-53 22.571562178035627 26.805238756384192 28.4154738489171 22.207725216663086 54-55 22.285604882002882 26.857696172316352 28.490792526585253 22.365906419095513 56-57 22.278248579788084 27.14798442863676 28.354854617686115 22.218912373889037 58-59 22.364620851595234 26.79307587238677 28.31853439161093 22.523768884407076 60-61 22.235138519831317 26.777711627663543 28.721951098575875 22.265198753929266 62-63 22.174046007634416 26.846717902587464 28.668743103607408 22.31049298617071 64-65 21.973608443975458 26.85459296831305 28.585789776750413 22.58600881096108 66-67 21.939160813875205 27.09132765243803 28.316100672030746 22.653410861656024 68-69 22.081408409081753 26.83338379209475 28.469832032599463 22.61537576622404 70-71 22.080387743407204 26.924700803367752 28.301632081813473 22.693279371411567 72-73 22.169553879702917 26.978709953111558 28.078765150422143 22.772971016763385 74-75 22.04808904255832 27.236774135781637 27.930146223198726 22.784990598461317 76-77 22.084160013334788 27.041967046678305 28.159409963139915 22.714462976846992 78-79 22.185067804420736 27.06976205941936 28.07293134005947 22.672238796100427 80-81 22.08373508912091 27.259566379239097 27.965164329595854 22.691534202044135 82-83 22.083235884836373 27.255107478085712 27.943475623638808 22.71818101343911 84-85 22.15840935601621 27.13070437028973 27.84343316587622 22.867453107817838 86-87 22.164526438587686 27.10278571209613 27.882505422182852 22.850182427133337 88-89 22.162683782695677 27.15339714075436 27.64894874799949 23.034970328550475 90-91 22.121392053321358 27.21932375668687 27.763090280121734 22.896193909870043 92-93 22.124855695184145 26.91908184941603 27.79410630202206 23.161956153377766 94-95 22.04899685246534 26.80539006701425 27.823001108868883 23.322611971651526 96-97 22.107910103955344 26.726272735819396 27.71737314999468 23.44844401023058 98-99 21.919523407772175 26.797706572082852 27.53628678179721 23.746483238347768 100-101 22.530199186010595 26.249866992381804 27.43407384858447 23.785859973023125 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 1454.0 1 974.5 2 752.5 3 1045.5 4 784.0 5 633.5 6 1232.0 7 2663.0 8 4216.0 9 4840.5 10 3847.5 11 2499.5 12 2204.5 13 2242.5 14 2345.5 15 2503.0 16 2553.0 17 2599.0 18 2781.5 19 2973.0 20 3295.5 21 3712.0 22 4308.5 23 5147.0 24 6083.5 25 7405.5 26 9135.5 27 10797.5 28 12128.5 29 13659.5 30 15760.0 31 18205.5 32 21143.5 33 23475.0 34 25235.5 35 27432.5 36 30464.5 37 33113.0 38 34722.5 39 36186.0 40 37815.0 41 40466.5 42 42622.0 43 44867.5 44 48196.5 45 50987.0 46 51925.0 47 52761.0 48 53953.0 49 53716.5 50 53470.5 51 52409.5 52 50907.0 53 51150.0 54 51270.0 55 48231.5 56 43943.5 57 40490.5 58 36942.0 59 32635.5 60 27421.5 61 22706.0 62 18749.5 63 14620.5 64 11160.5 65 8579.5 66 6404.0 67 5014.5 68 3770.5 69 2630.0 70 1894.0 71 1297.0 72 931.0 73 741.5 74 555.5 75 437.5 76 292.0 77 150.5 78 86.5 79 47.0 80 24.0 81 12.0 82 6.0 83 2.5 84 1.5 85 1.0 86 2.0 87 2.5 88 1.0 89 0.5 90 0.5 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.008952360358672876 2 0.01404831933207128 3 0.008814631737770216 4 6.886431045132981E-5 5 6.88643104513298E-4 6 0.0017904720717345749 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 5.164823283849735E-4 14-15 0.0 16-17 0.0027201402628275273 18-19 0.0 20-21 0.0 22-23 0.0 24-25 0.0034087833673408256 26-27 0.019557464168177666 28-29 0.00723075259738963 30-31 0.0036842406091461446 32-33 0.0 34-35 0.008435878030287901 36-37 7.575074149646278E-4 38-39 0.0026857081076018625 40-41 0.0019970650030885643 42-43 0.0029955975046328464 44-45 0.0 46-47 6.886431045132981E-5 48-49 6.88643104513298E-4 50-51 0.003890833540500134 52-53 0.007368481218292289 54-55 0.0071618882869382994 56-57 0.016183112956062503 58-59 0.023276136932549474 60-61 0.003202190435986836 62-63 0.019626328478628996 64-65 0.020865886066752933 66-67 0.012980922520075669 68-69 0.031023371858324076 70-71 0.01673402743967314 72-73 0.004648340955464762 74-75 0.016596298818770484 76-77 0.019936217875659978 78-79 0.026306166592407986 80-81 0.011775797087177397 82-83 0.03508636617495253 84-85 0.021795554257845885 86-87 0.03136769341058073 88-89 0.021657825636943222 90-91 0.027890045732788568 92-93 0.023620458484806123 94-95 0.01384172640071729 96-97 0.014427073039553593 98-99 0.01074283243040745 100-101 0.010088621481119817 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1452131.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 60.67080995907808 #Duplication Level Percentage of deduplicated Percentage of total 1 80.94197287550165 49.10815054042412 2 11.39870366497551 13.831371676751516 3 3.1492771369988555 5.732075840619812 4 1.3812976358759042 3.352177854526032 5 0.7644487249591054 2.318986165772672 6 0.4639326633370189 1.6888302270677529 7 0.3261172570751184 1.3850058689866225 8 0.2386421441217108 1.158288973938819 9 0.18698399608760743 1.0210023442818206 >10 1.050156096402839 11.719451986556358 >50 0.06813155498518637 2.8362961267487505 >100 0.02772307940471593 3.1050832532386687 >500 0.001931473681396645 0.8212535539591371 >1k 3.4084829671705505E-4 0.3484502866596809 >5k 3.4084829671705505E-4 1.573575300468288 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9098 0.6265274964861985 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8567 0.5899605476365424 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5163 0.3555464348602158 No Hit GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2211 0.1522589904078902 No Hit ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1623 0.11176677586250829 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 6.88643104513298E-5 0.0 0.0 6.88643104513298E-5 0.0 8 6.88643104513298E-5 0.0 0.0 6.88643104513298E-5 0.0 9 6.88643104513298E-5 0.0 0.0 6.88643104513298E-5 0.0 10-11 6.88643104513298E-5 0.0 0.0 6.88643104513298E-5 0.0 12-13 1.377286209026596E-4 0.0 0.0 6.88643104513298E-5 3.44321552256649E-5 14-15 1.721607761283245E-4 0.0 0.0 6.88643104513298E-5 6.88643104513298E-5 16-17 2.0659293135398942E-4 0.0 0.0 6.88643104513298E-5 6.88643104513298E-5 18-19 2.0659293135398942E-4 0.0 0.0 1.032964656769947E-4 6.88643104513298E-5 20-21 2.754572418053192E-4 0.0 0.0 1.721607761283245E-4 6.88643104513298E-5 22-23 2.754572418053192E-4 0.0 0.0 2.4102508657965432E-4 6.88643104513298E-5 24-25 3.7875370748231395E-4 0.0 0.0 6.197787940619683E-4 6.88643104513298E-5 26-27 4.1318586270797883E-4 0.0 0.0 0.0012739897433496014 6.88643104513298E-5 28-29 4.1318586270797883E-4 0.0 0.0 0.004269587247982447 6.88643104513298E-5 30-31 4.1318586270797883E-4 0.0 0.0 0.01208568648420838 6.88643104513298E-5 32-33 4.1318586270797883E-4 0.0 0.0 0.028785281768655857 6.88643104513298E-5 34-35 5.509144836106385E-4 0.0 0.0 0.05440280525655054 6.88643104513298E-5 36-37 6.197787940619683E-4 0.0 0.0 0.0970298134259237 6.88643104513298E-5 38-39 7.575074149646278E-4 0.0 0.0 0.1616934009397224 6.88643104513298E-5 40-41 7.575074149646278E-4 0.0 0.0 0.22797529974912734 6.88643104513298E-5 42-43 7.575074149646278E-4 0.0 0.0 0.2930176409704083 6.88643104513298E-5 44-45 8.608038806416226E-4 0.0 0.0 0.3777551749807696 6.88643104513298E-5 46-47 9.641003463186173E-4 0.0 0.0 0.4734421343528924 6.88643104513298E-5 48-49 9.641003463186173E-4 0.0 0.0 0.5743972134745419 6.88643104513298E-5 50-51 9.641003463186173E-4 0.0 0.0 0.6812057589845544 6.88643104513298E-5 52-53 9.641003463186173E-4 0.0 0.0 0.7788209190493145 6.88643104513298E-5 54-55 9.641003463186173E-4 0.0 0.0 0.8855606002488756 6.88643104513298E-5 56-57 9.641003463186173E-4 0.0 0.0 1.0122364993240969 6.88643104513298E-5 58-59 9.641003463186173E-4 0.0 0.0 1.1514801350566857 6.88643104513298E-5 60-61 0.001067396811995612 0.0 0.0 1.3010878495121996 6.88643104513298E-5 62-63 0.0011018289672212768 0.0 0.0 1.444084590164386 6.88643104513298E-5 64-65 0.0011018289672212768 0.0 0.0 1.5861516626254795 6.88643104513298E-5 66-67 0.0011018289672212768 0.0 0.0 1.7516670327952506 6.88643104513298E-5 68-69 0.0011018289672212768 0.0 0.0 1.9383926105840312 6.88643104513298E-5 70-71 0.0011362611224469419 0.0 0.0 2.1353101063196087 6.88643104513298E-5 72-73 0.0012051254328982715 0.0 0.0 2.3326407879178945 6.88643104513298E-5 74-75 0.0012395575881239366 0.0 0.0 2.530178062447534 6.88643104513298E-5 76-77 0.0012395575881239366 0.0 0.0 2.742899917431692 6.88643104513298E-5 78-79 0.0012395575881239366 0.0 0.0 2.9780715376229834 6.88643104513298E-5 80-81 0.001446150519477926 0.0 0.0 3.2312856071525227 6.88643104513298E-5 82-83 0.001446150519477926 0.0 0.0 3.4846374053029647 6.88643104513298E-5 84-85 0.0015150148299292556 0.0 0.0 3.7390910324206286 6.88643104513298E-5 86-87 0.0015150148299292556 0.0 0.0 4.00301350222535 6.88643104513298E-5 88-89 0.0015150148299292556 0.0 0.0 4.28783629025205 6.88643104513298E-5 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 3605 0.0 59.301464 1 GTATCAA 6340 0.0 41.58741 1 GTGGTAT 1400 0.0 37.666313 1 TGGTATC 1495 0.0 33.046116 2 ATCAACG 8230 0.0 31.970427 3 TCAACGC 8305 0.0 31.73562 4 TATCAAC 8330 0.0 31.707209 2 CAACGCA 8525 0.0 30.916637 5 AACGCAG 8700 0.0 30.349337 6 ACGCAGA 10100 0.0 26.049356 7 GGACCGA 310 0.0 26.04239 6 CGCAGAG 10105 0.0 25.848476 8 GTACCTG 1185 0.0 23.653305 1 TACCTGG 1145 0.0 23.648174 2 GCAGAGT 11055 0.0 23.369465 9 GTACATG 6575 0.0 23.049053 1 GAGTACT 7200 0.0 22.231348 12-13 TACATGG 6715 0.0 22.213238 2 ACATGGG 6640 0.0 21.815758 3 GTGTACG 175 4.0452396E-6 21.717514 1 >>END_MODULE