FastQCFastQC Report
Thu 26 May 2016
SRR938115_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938115_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988451
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106110.5336314548359502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83170.4182652728178869No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA82950.4171588839755166No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA65310.3284466149781916No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57530.2893206822798249No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT51140.2571851154491612No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG30390.15283253145287462No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT27060.13608582761154286No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26890.1352308907788022No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA26810.1348285675633948No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC23410.11772983090858159No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG20880.10500635922132354No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC16300.026.0560591
GAGTACT72250.023.40648712-13
TAGCCCT24850.023.332714
TAGGACG7550.022.6614284
ATAGCCC15350.021.9827333
GTCCTAC9350.021.9463921
GTGTAGC16450.021.7571321
TATAGCC17600.021.3327182
AGTACTT74650.021.2858212-13
GTACTTT80300.021.20843514-15
GTACATG89400.020.9778651
AGGACGT7950.020.3256075
GGACGTG8250.020.1590166
GTATCAA182950.019.9803311
TACATGG90850.019.7741852
GACGACC3800.019.39026394-95
GCGCCGT1001.2773302E-519.01496794-95
GTAGCCC14550.018.9450933
TCGAGAC2803.470268E-818.670953
GTAGGAC10800.018.4823533
Produced by FastQC (version 0.11.5)