##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938115_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1988451 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.97836356037941 33.0 31.0 34.0 30.0 34.0 2 32.13965242291613 34.0 31.0 34.0 30.0 34.0 3 32.23641165912562 34.0 31.0 34.0 30.0 34.0 4 35.74689293324301 37.0 35.0 37.0 35.0 37.0 5 35.77413172363815 37.0 35.0 37.0 35.0 37.0 6 35.80940993768516 37.0 36.0 37.0 35.0 37.0 7 35.75646571124961 37.0 35.0 37.0 35.0 37.0 8 35.73286341981774 37.0 35.0 37.0 35.0 37.0 9 37.38914159815857 39.0 37.0 39.0 35.0 39.0 10-11 37.28877301980285 39.0 37.0 39.0 34.0 39.0 12-13 37.17903181924021 39.0 37.0 39.0 34.0 39.0 14-15 38.34170643380199 40.0 38.0 41.0 34.0 41.0 16-17 38.18285590140265 40.0 38.0 41.0 33.0 41.0 18-19 38.40104382758237 40.0 38.0 41.0 34.0 41.0 20-21 38.40479498866203 40.0 38.0 41.0 34.0 41.0 22-23 38.301724558462844 40.0 38.0 41.0 34.0 41.0 24-25 38.19765636668945 40.0 38.0 41.0 34.0 41.0 26-27 37.99543715183326 40.0 38.0 41.0 33.0 41.0 28-29 37.798078504323215 40.0 38.0 41.0 33.0 41.0 30-31 37.58400106414491 40.0 37.0 41.0 32.5 41.0 32-33 37.30619411793401 40.0 36.5 41.0 32.0 41.0 34-35 37.04803286578347 39.5 36.0 41.0 31.0 41.0 36-37 36.770550795569015 39.0 36.0 41.0 30.5 41.0 38-39 36.46976163858199 39.0 35.0 40.5 30.0 41.0 40-41 36.331964177140904 39.0 35.0 40.0 30.0 41.0 42-43 36.12327384481689 39.0 35.0 40.5 29.5 41.0 44-45 35.773004967183 39.0 35.0 40.0 28.0 41.0 46-47 35.33112256726467 38.5 35.0 40.0 26.5 41.0 48-49 34.8904690133174 38.0 34.5 40.0 24.0 41.0 50-51 34.11356251675299 37.5 33.5 39.5 23.5 40.5 52-53 34.105900774019574 38.0 33.5 40.0 23.0 40.5 54-55 34.13631942652849 38.0 33.5 40.0 22.5 41.0 56-57 33.729873906875255 38.0 33.0 40.0 18.5 41.0 58-59 33.32490767939466 38.0 33.0 40.0 16.0 41.0 60-61 32.806519748286476 37.5 31.5 40.0 10.5 41.0 62-63 32.212321802247075 37.0 31.0 40.0 7.5 41.0 64-65 31.62517306184563 36.0 30.5 39.0 2.0 41.0 66-67 30.967403270183674 36.0 29.5 39.0 2.0 40.0 68-69 30.27810868862245 35.0 28.5 38.5 2.0 40.0 70-71 29.436519180004936 35.0 27.5 38.0 2.0 40.0 72-73 28.587551566520872 34.0 26.0 37.5 2.0 39.5 74-75 27.743339161990917 34.0 25.5 36.5 2.0 39.0 76-77 26.838412915379863 33.0 22.5 36.0 2.0 39.0 78-79 25.95989290156006 32.0 19.0 35.5 2.0 38.0 80-81 25.11762975300875 32.0 14.0 35.0 2.0 37.0 82-83 24.234746041013835 31.0 4.5 35.0 2.0 36.5 84-85 23.357920813738936 30.5 2.0 34.5 2.0 36.0 86-87 22.4975689619709 30.0 2.0 34.0 2.0 35.5 88-89 21.603668885982103 29.0 2.0 34.0 2.0 35.0 90-91 20.68426076378045 29.0 2.0 34.0 2.0 35.0 92-93 19.752505845001963 26.5 2.0 34.0 2.0 35.0 94-95 18.765717887943932 25.5 2.0 34.0 2.0 35.0 96-97 17.598274234567512 22.0 2.0 33.0 2.0 35.0 98-99 16.24433491194905 6.0 2.0 32.0 2.0 34.5 100-101 13.153522012863279 2.0 2.0 28.5 2.0 32.5 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5093662086027884 1101 2 0.42521824643809936 1101 3 0.49614294017422367 1101 4 0.42768824834857355 1101 5 0.32037851132127315 1101 6 0.23698347930192654 1101 7 0.3722345270756193 1101 8 0.40059853610040363 1101 9 0.37334918939899353 1101 10-11 0.4450586200063995 1101 12-13 0.4935835489667042 1101 14-15 0.6553073848566839 1101 16-17 0.6651250052341311 1101 18-19 0.6976517210910629 1101 20-21 0.5717409458706939 1101 22-23 0.6367841530673459 1101 24-25 0.5519713719507209 1101 26-27 0.6504124825637447 1101 28-29 0.5807127606239462 1101 30-31 0.6330393119243141 1101 32-33 0.8539613979233636 1101 34-35 0.8963521348873158 1101 36-37 0.940520001421639 1101 38-39 1.0817327595817758 1101 40-41 1.211246624637674 1101 42-43 1.4855487039229942 1101 44-45 1.5698298554661463 1101 46-47 1.6789823879151555 1101 48-49 1.7166291335980333 1101 50-51 2.0291707381864796 1101 52-53 2.0359499529247316 1101 54-55 2.130786412244966 1101 56-57 2.239208558187549 1101 58-59 2.587962191778452 1101 60-61 2.7325665955590566 1101 62-63 2.9897689014691053 1101 64-65 2.91042093884311 1101 66-67 3.114551638080215 1101 68-69 3.2742687208955914 1101 70-71 3.474524892874296 1101 72-73 4.097078752180593 1101 74-75 4.480284395857595 1101 76-77 4.512817443356962 1101 78-79 4.64686939394694 1101 80-81 4.783583212277588 1101 82-83 5.017511244617548 1101 84-85 5.322521174180096 1101 86-87 5.406544582609445 1101 88-89 5.675452628749785 1101 90-91 5.679167089420925 1101 92-93 6.103233066236626 1101 94-95 5.885862931303976 1101 96-97 5.7685239455584885 1101 98-99 5.3282918523467195 1101 100-101 4.842253614015894 1102 1 0.5046167265673169 1102 2 0.4366305209340311 1102 3 0.43892238835250197 1102 4 0.3008267343761659 1102 5 0.34106266666010043 1102 6 0.3114355107335314 1102 7 0.3709212975036351 1102 8 0.39392532118302626 1102 9 0.5897895097790027 1102 10-11 0.5693963202439036 1102 12-13 0.5312894714488863 1102 14-15 0.7713606530865107 1102 16-17 0.8226553882752086 1102 18-19 0.6980176124953772 1102 20-21 0.6484071177338535 1102 22-23 0.700008671739333 1102 24-25 0.6315204585644949 1102 26-27 0.8246492744683351 1102 28-29 0.8311672429120875 1102 30-31 0.8116168992202901 1102 32-33 0.8636823919099257 1102 34-35 1.0321231124506127 1102 36-37 1.0946326081728657 1102 38-39 1.2777728576160357 1102 40-41 1.2470245505055786 1102 42-43 1.3284579590647425 1102 44-45 1.430219939603795 1102 46-47 1.5387090668782122 1102 48-49 1.753200903268052 1102 50-51 1.8732433606029772 1102 52-53 1.9185997204723577 1102 54-55 2.006581993123227 1102 56-57 2.2326998917567025 1102 58-59 2.414269894289937 1102 60-61 2.4977625377491606 1102 62-63 2.63925062111133 1102 64-65 2.727095922419842 1102 66-67 2.9538239295047504 1102 68-69 3.049756681378689 1102 70-71 3.4269907514320828 1102 72-73 3.5848601585174507 1102 74-75 3.8520226405895173 1102 76-77 3.9621867267972597 1102 78-79 4.122675713114152 1102 80-81 4.398306827669977 1102 82-83 4.752440687742517 1102 84-85 4.94657557308858 1102 86-87 5.213428615405444 1102 88-89 5.2083615364763 1102 90-91 5.386463598845353 1102 92-93 5.703092994732259 1102 94-95 5.769100936003557 1102 96-97 5.832053242137395 1102 98-99 6.181982665424691 1102 100-101 5.2159923388125 1103 1 0.5457160632685181 1103 2 0.36561992028795487 1103 3 0.3202496355736457 1103 4 0.3433430042872274 1103 5 0.2640132462115261 1103 6 0.2883322063741929 1103 7 0.36207741571949725 1103 8 0.28190241001039595 1103 9 0.3725761614346723 1103 10-11 0.4441809764261606 1103 12-13 0.5745560356861574 1103 14-15 0.6379440115981936 1103 16-17 0.7198552598196812 1103 18-19 0.7248270368172314 1103 20-21 0.669531802340579 1103 22-23 0.7201458850509042 1103 24-25 0.8540220259549258 1103 26-27 0.8424927045743686 1103 28-29 0.8767104302641187 1103 30-31 0.9009639761520347 1103 32-33 1.0105759864770434 1103 34-35 1.058553635839509 1103 36-37 1.0335135741388868 1103 38-39 1.0855352553219788 1103 40-41 1.27059531412349 1103 42-43 1.2444999167124138 1103 44-45 1.467406469177682 1103 46-47 1.654521753455903 1103 48-49 1.5933048627751418 1103 50-51 1.8011052500637987 1103 52-53 1.7796936885165948 1103 54-55 1.7702365224985428 1103 56-57 2.1295162659153206 1103 58-59 2.383611247105698 1103 60-61 2.507800884423439 1103 62-63 2.663164586544781 1103 64-65 2.7251554532261935 1103 66-67 3.0224820655634232 1103 68-69 3.188943096960543 1103 70-71 3.349037208700704 1103 72-73 3.519343085146417 1103 74-75 3.785713445171016 1103 76-77 4.145311554813311 1103 78-79 4.164936573459819 1103 80-81 4.308686261791468 1103 82-83 4.524401467129323 1103 84-85 4.7851464623428726 1103 86-87 4.9604000125984165 1103 88-89 5.192421344295539 1103 90-91 5.066906703032235 1103 92-93 5.199796725152133 1103 94-95 5.229218586811644 1103 96-97 5.071581603170994 1103 98-99 4.8639425990641705 1103 100-101 4.055208723267173 1104 1 0.5312335467115368 1104 2 0.4233811953347839 1104 3 0.3453190615145445 1104 4 0.27842078524583513 1104 5 0.28456262506581 1104 6 0.24971634070281823 1104 7 0.3722279168164917 1104 8 0.37671116289718753 1104 9 0.3914006051963099 1104 10-11 0.44163540718009386 1104 12-13 0.4199434595479232 1104 14-15 0.718544278515644 1104 16-17 0.7512960426842525 1104 18-19 0.6827054745163821 1104 20-21 0.7299849906281608 1104 22-23 0.5525261847004401 1104 24-25 0.5630325889595156 1104 26-27 0.7704576426521967 1104 28-29 0.7131736399085966 1104 30-31 0.7577284795124157 1104 32-33 0.880015133320704 1104 34-35 1.0204692145950176 1104 36-37 1.1250386243243824 1104 38-39 1.3100961796096584 1104 40-41 1.2974274272433206 1104 42-43 1.4428034633095663 1104 44-45 1.3499796980217909 1104 46-47 1.584566554541766 1104 48-49 1.5611603460397419 1104 50-51 1.7882623984432016 1104 52-53 1.7800575078499747 1104 54-55 1.7264401668721447 1104 56-57 2.1602123560818782 1104 58-59 2.4508974172483775 1104 60-61 2.40551080497206 1104 62-63 2.5871425253333413 1104 64-65 2.8923932598853668 1104 66-67 3.0221905436616794 1104 68-69 3.25155617101818 1104 70-71 3.3871701683418216 1104 72-73 3.716218757468802 1104 74-75 4.00269392544752 1104 76-77 4.0728983889845765 1104 78-79 4.14787685753042 1104 80-81 4.487339191557428 1104 82-83 4.3507305009033 1104 84-85 4.718509948758239 1104 86-87 4.768163765581416 1104 88-89 4.892277390780453 1104 90-91 5.2633290587973995 1104 92-93 5.289322226613535 1104 94-95 5.3863621561008586 1104 96-97 5.533848028535264 1104 98-99 5.337471098536806 1104 100-101 4.459131558585865 1105 1 0.46457080130738504 1105 2 0.3371276208474754 1105 3 0.34921183074311557 1105 4 0.266889250049573 1105 5 0.3146955118882744 1105 6 0.25742739349583843 1105 7 0.29866384100493093 1105 8 0.30431357106543544 1105 9 0.3079763865261853 1105 10-11 0.3718883780861617 1105 12-13 0.4173148851980386 1105 14-15 0.46740280632710807 1105 16-17 0.5295185508776754 1105 18-19 0.47683039714473807 1105 20-21 0.5526005423552931 1105 22-23 0.5641324804156724 1105 24-25 0.6536871392914847 1105 26-27 0.6833983311745939 1105 28-29 0.7496328607940441 1105 30-31 0.7523462718562755 1105 32-33 0.7749132501252163 1105 34-35 0.8193067984669753 1105 36-37 0.9855286245552648 1105 38-39 0.7974410377900512 1105 40-41 0.8816285231445775 1105 42-43 0.9519858141101096 1105 44-45 1.0045285182898098 1105 46-47 1.1853835880663581 1105 48-49 1.2226594208783226 1105 50-51 1.4104118788026199 1105 52-53 1.4164534021781776 1105 54-55 1.5620623997709444 1105 56-57 1.8626587566377495 1105 58-59 2.0635475017387606 1105 60-61 2.1970416475340997 1105 62-63 2.222670290620492 1105 64-65 2.36708201425418 1105 66-67 2.4145215161750926 1105 68-69 2.6393450297218948 1105 70-71 3.011763245737651 1105 72-73 3.324690498542342 1105 74-75 3.3955739205221214 1105 76-77 3.692212879002401 1105 78-79 3.8084917243192713 1105 80-81 3.998827849397223 1105 82-83 4.264294697651 1105 84-85 4.036698550857473 1105 86-87 4.371286006725867 1105 88-89 4.578096859519018 1105 90-91 4.579480711609548 1105 92-93 4.901380273758836 1105 94-95 4.639308050374094 1105 96-97 4.45430050008504 1105 98-99 4.477353938908802 1105 100-101 3.766834860472139 1106 1 0.3543639975521877 1106 2 0.3426814731755172 1106 3 0.2686904195765436 1106 4 0.1846012726003039 1106 5 0.302584499777268 1106 6 0.22715332289919132 1106 7 0.1389843990674251 1106 8 0.1672561840080462 1106 9 0.2814142873834413 1106 10-11 0.23636482724003116 1106 12-13 0.37174942430999636 1106 14-15 0.5797498218999309 1106 16-17 0.3500889883835967 1106 18-19 0.402229032220788 1106 20-21 0.4261271174947652 1106 22-23 0.39773699950406893 1106 24-25 0.35591533345516524 1106 26-27 0.46971393603858047 1106 28-29 0.5301009396492233 1106 30-31 0.5766157735451358 1106 32-33 0.5348756673457018 1106 34-35 0.43434278447070795 1106 36-37 0.6339236519825491 1106 38-39 0.7489376425124661 1106 40-41 0.7614172537397579 1106 42-43 0.6456901529757388 1106 44-45 0.883685724059923 1106 46-47 0.8752500763199436 1106 48-49 1.11510845445639 1106 50-51 1.274840424682779 1106 52-53 1.2642138709063957 1106 54-55 1.4374396380514085 1106 56-57 1.3490349325300528 1106 58-59 1.5918197477529432 1106 60-61 1.825046114248245 1106 62-63 1.7686245664134859 1106 64-65 2.0669292826498378 1106 66-67 2.2049610606468946 1106 68-69 2.177886406972952 1106 70-71 2.4798397057495976 1106 72-73 2.8884926026670286 1106 74-75 2.8921703929250455 1106 76-77 2.982071330151175 1106 78-79 3.342331794449663 1106 80-81 3.314519737669759 1106 82-83 3.2634792774807444 1106 84-85 3.5249783496211435 1106 86-87 3.7124025575198374 1106 88-89 4.008873967715477 1106 90-91 4.185059393801133 1106 92-93 4.247167659868801 1106 94-95 4.051064631485513 1106 96-97 4.011100343175205 1106 98-99 3.8322164671584282 1106 100-101 3.284028942182351 1107 1 0.5374892960280526 1107 2 0.4228778434438851 1107 3 0.36621555916579496 1107 4 0.2978726794017206 1107 5 0.3148817715255987 1107 6 0.3664315396569009 1107 7 0.3512544649316496 1107 8 0.33155135141041825 1107 9 0.4726377115966258 1107 10-11 0.4582427332779062 1107 12-13 0.4054856486795302 1107 14-15 0.5104820764907885 1107 16-17 0.46599096022683284 1107 18-19 0.5538250709804231 1107 20-21 0.5953866958353871 1107 22-23 0.5652879899865084 1107 24-25 0.6727617949534022 1107 26-27 0.6899202446747523 1107 28-29 0.7490931923362041 1107 30-31 0.7686159973440212 1107 32-33 0.7939215575203704 1107 34-35 0.8468904511153639 1107 36-37 1.0554532378511894 1107 38-39 0.9794158959689483 1107 40-41 1.0353025483230027 1107 42-43 0.9619437065123293 1107 44-45 1.0505285925783383 1107 46-47 1.3141834730806465 1107 48-49 1.424380357645866 1107 50-51 1.4658611305879674 1107 52-53 1.5620048114530505 1107 54-55 1.677462357351125 1107 56-57 1.8510112040140214 1107 58-59 2.014109295257562 1107 60-61 2.2095409611727277 1107 62-63 2.326015723652624 1107 64-65 2.5437835983224204 1107 66-67 2.75569009083668 1107 68-69 2.8066497881711427 1107 70-71 3.1349721116963956 1107 72-73 3.3428290111137926 1107 74-75 3.4977984312137487 1107 76-77 3.471175983444528 1107 78-79 3.574213033606089 1107 80-81 3.8038837219051906 1107 82-83 3.9967515699317993 1107 84-85 4.034962325726632 1107 86-87 4.237692627663819 1107 88-89 4.319621776859574 1107 90-91 4.524914847294848 1107 92-93 4.351866163783825 1107 94-95 4.549603309581837 1107 96-97 4.623839432117499 1107 98-99 4.501279823722829 1107 100-101 3.646949867723345 1108 1 0.2945662630393677 1108 2 0.22850485503907692 1108 3 0.2598133266739495 1108 4 0.1232448623932072 1108 5 0.12033428159890036 1108 6 0.0496842141957714 1108 7 0.07305788036902783 1108 8 0.08440025085271685 1108 9 0.09438212353072828 1108 10-11 0.2477906559345442 1108 12-13 0.22921909605435076 1108 14-15 0.3211093037461836 1108 16-17 0.303485783677246 1108 18-19 0.3682780820055953 1108 20-21 0.36779259257728114 1108 22-23 0.3300067986851971 1108 24-25 0.3086381520269299 1108 26-27 0.5075555827270506 1108 28-29 0.4639701095624318 1108 30-31 0.3680811600462519 1108 32-33 0.4512225574225468 1108 34-35 0.5855039449036425 1108 36-37 0.6635653879213734 1108 38-39 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26-27 0.1370931777751494 2311 28-29 0.2016551285461432 2311 30-31 0.17752104562578808 2311 32-33 0.15137496010914475 2311 34-35 0.30266111659010875 2311 36-37 0.44038478136065606 2311 38-39 0.5667684061654725 2311 40-41 0.5655723787792724 2311 42-43 0.5793327021097596 2311 44-45 0.7993719617053259 2311 46-47 0.9219751555736124 2311 48-49 1.199153550477618 2311 50-51 1.215841646041092 2311 52-53 1.2218772792249055 2311 54-55 1.3490151067145888 2311 56-57 1.5390231135324797 2311 58-59 1.496025170843808 2311 60-61 1.766553449593502 2311 62-63 2.0195507979497833 2311 64-65 2.120639454461667 2311 66-67 2.064542108285444 2311 68-69 2.3662889343629985 2311 70-71 2.978956762568526 2311 72-73 3.191882695970264 2311 74-75 3.566182617649403 2311 76-77 3.563643187196078 2311 78-79 3.6800377158788606 2311 80-81 3.8520032753287694 2311 82-83 4.200509575615651 2311 84-85 4.315228047995312 2311 86-87 4.312310108448067 2311 88-89 4.817409224398016 2311 90-91 4.977330414649639 2311 92-93 5.268365607652601 2311 94-95 5.0494557669588005 2311 96-97 5.02660778714754 2311 98-99 4.927952900727167 2311 100-101 4.850573124854103 2312 1 -0.42053359589379014 2312 2 -0.32161704192944995 2312 3 -0.24545448569739392 2312 4 -0.2082558702568349 2312 5 -0.2521390025268815 2312 6 -0.16935205344489646 2312 7 -0.17745185285269827 2312 8 -0.11448984773798543 2312 9 -0.15650173924225896 2312 10-11 -0.16596492186391032 2312 12-13 -0.10757725259732354 2312 14-15 -0.04035386473601932 2312 16-17 -0.013070253807903498 2312 18-19 -0.05440819256159557 2312 20-21 -0.04830324604849778 2312 22-23 0.08459695854144655 2312 24-25 0.056312158851987704 2312 26-27 0.11378715672470463 2312 28-29 0.1289552663261304 2312 30-31 0.19111910175814018 2312 32-33 0.10960044973177929 2312 34-35 0.32182358324183014 2312 36-37 0.3764671747680097 2312 38-39 0.5135305764602407 2312 40-41 0.6949169977233751 2312 42-43 0.7452485247115277 2312 44-45 0.8841657547818826 2312 46-47 0.7767157701243477 2312 48-49 1.0262067647482453 2312 50-51 1.1508644902229719 2312 52-53 1.2628377828367547 2312 54-55 1.3285686703094726 2312 56-57 1.389933550041576 2312 58-59 1.4443863120130516 2312 60-61 1.7357924020106665 2312 62-63 1.7892658874580292 2312 64-65 1.9355288730236282 2312 66-67 2.04424677493261 2312 68-69 2.297446058790669 2312 70-71 2.6989577293030997 2312 72-73 2.8162319779870444 2312 74-75 2.5501005004519257 2312 76-77 2.873808401323725 2312 78-79 3.314426008479362 2312 80-81 3.451904530296485 2312 82-83 3.77592807420373 2312 84-85 4.14018572289303 2312 86-87 4.407967073648869 2312 88-89 4.485320460291003 2312 90-91 4.769832819300365 2312 92-93 4.850790291717239 2312 94-95 4.961138876237179 2312 96-97 4.916086262274028 2312 98-99 4.762676016569593 2312 100-101 4.331615987753269 2313 1 -0.48386848690363493 2313 2 -0.3186090741780738 2313 3 -0.42500699198317093 2313 4 -0.19213077152532065 2313 5 -0.16791315201977142 2313 6 -0.1916256475238427 2313 7 -0.09933044219567222 2313 8 -0.08393185339596698 2313 9 -0.10854182345119767 2313 10-11 -0.16046506504428493 2313 12-13 -0.19949550762200374 2313 14-15 -0.20314974460296753 2313 16-17 -0.09648855131467826 2313 18-19 -0.14752322303855436 2313 20-21 -0.003557611476260547 2313 22-23 0.005959665877625753 2313 24-25 -0.06442783189636003 2313 26-27 -0.048872426447353234 2313 28-29 -0.040277043031572646 2313 30-31 -0.001663427719712729 2313 32-33 0.1400571557651702 2313 34-35 0.15628583869447965 2313 36-37 0.22305826265557727 2313 38-39 0.2423351620081462 2313 40-41 0.4302343231770678 2313 42-43 0.45944576274653315 2313 44-45 0.6366904887968232 2313 46-47 0.6443232970060677 2313 48-49 0.6458807698020408 2313 50-51 0.8287090592815431 2313 52-53 0.9810877332743217 2313 54-55 1.177477588715547 2313 56-57 1.3246263888204837 2313 58-59 1.3269569738839593 2313 60-61 1.4268927588601912 2313 62-63 1.4650038563737269 2313 64-65 1.8692210226486736 2313 66-67 1.7463895241844511 2313 68-69 1.9976908814830239 2313 70-71 2.3088520039765434 2313 72-73 2.798916987474712 2313 74-75 2.843493001891595 2313 76-77 2.8337483835756565 2313 78-79 3.0233843791811985 2313 80-81 3.2935263940957675 2313 82-83 3.6299019980695384 2313 84-85 3.7219275513849794 2313 86-87 3.869030864435608 2313 88-89 3.719583993982443 2313 90-91 4.007844807115873 2313 92-93 4.110990319409268 2313 94-95 4.1411374444335145 2313 96-97 4.176920964474601 2313 98-99 4.204972952195071 2313 100-101 3.7171820300905996 2314 1 -0.3933346921913312 2314 2 -0.33431663957983915 2314 3 -0.23634145396271578 2314 4 -0.18126783793802304 2314 5 -0.14661977993549158 2314 6 -0.053145565382202165 2314 7 -0.21202775019513354 2314 8 -0.15581246109449154 2314 9 -0.1437337982348481 2314 10-11 -0.284481649046576 2314 12-13 -0.25711427016331356 2314 14-15 -0.17780433040545063 2314 16-17 -0.11217513107024502 2314 18-19 -0.12814746207040884 2314 20-21 -0.17079527849815435 2314 22-23 -0.1725536842365969 2314 24-25 -0.13928477656178728 2314 26-27 -0.14634226339651235 2314 28-29 -0.078353208717445 2314 30-31 0.07823865707069189 2314 32-33 0.08855332435851437 2314 34-35 0.2194148595919856 2314 36-37 0.33759353170491835 2314 38-39 0.30789184061373476 2314 40-41 0.33967114856007896 2314 42-43 0.4904297339099557 2314 44-45 0.7600358182886353 2314 46-47 0.7192907024819419 2314 48-49 0.644601408498275 2314 50-51 0.7415619663086446 2314 52-53 0.9254888391797209 2314 54-55 0.9972873900806789 2314 56-57 1.2195056622134715 2314 58-59 1.464592288376764 2314 60-61 1.603345604685515 2314 62-63 1.5900032363432715 2314 64-65 1.9244146076037225 2314 66-67 2.0390193843170294 2314 68-69 2.4629117515937082 2314 70-71 2.8639984666169447 2314 72-73 2.9860862489211435 2314 74-75 3.2176459925941927 2314 76-77 3.4815289225557287 2314 78-79 3.790962624163054 2314 80-81 4.023861031210064 2314 82-83 4.150560363577686 2314 84-85 4.526457703752218 2314 86-87 4.6236146065120955 2314 88-89 4.873185951218279 2314 90-91 4.908484258833436 2314 92-93 5.198075600115178 2314 94-95 5.4526882493475135 2314 96-97 5.272028103829594 2314 98-99 5.175356863606645 2314 100-101 4.766852479133826 2315 1 -0.4265148080235335 2315 2 -0.30543579506819185 2315 3 -0.2651081014511085 2315 4 -0.1682766935188127 2315 5 -0.15609092441540184 2315 6 -0.0423960592084498 2315 7 -0.1489407239064846 2315 8 -0.17066376499876412 2315 9 -0.26390776469013133 2315 10-11 -0.33807324976067576 2315 12-13 -0.29379740813817534 2315 14-15 -0.3319494452294194 2315 16-17 -0.215854172179462 2315 18-19 -0.3765178307124373 2315 20-21 -0.3152324133214037 2315 22-23 -0.321358380959083 2315 24-25 -0.1880058362949839 2315 26-27 -0.1844429051192904 2315 28-29 -0.051293161760078476 2315 30-31 -0.014974283766328256 2315 32-33 -0.16209178449002337 2315 34-35 -0.005853754759918672 2315 36-37 0.11273228962448911 2315 38-39 0.09187868633071616 2315 40-41 0.010159242192536055 2315 42-43 0.3025145485323364 2315 44-45 0.24482873943900785 2315 46-47 0.4762744100571368 2315 48-49 0.6994311553431984 2315 50-51 0.7832128644909915 2315 52-53 0.8607656844433222 2315 54-55 0.9932400351170685 2315 56-57 1.3085431084038106 2315 58-59 1.584298274671987 2315 60-61 1.581775456318546 2315 62-63 1.683013961188955 2315 64-65 1.8820691373272922 2315 66-67 1.8577978138272115 2315 68-69 2.270917911403906 2315 70-71 2.64668807588291 2315 72-73 2.7866438984049537 2315 74-75 3.142356957462294 2315 76-77 3.353885010120184 2315 78-79 3.6184362464527915 2315 80-81 3.823862491869665 2315 82-83 3.9546880927584915 2315 84-85 4.196770707928756 2315 86-87 4.491036889462347 2315 88-89 4.668148231106837 2315 90-91 4.83282778244898 2315 92-93 4.898978894775176 2315 94-95 5.277757474179257 2315 96-97 5.454072386096559 2315 98-99 5.168394923653413 2315 100-101 4.9712535792950465 2316 1 -0.6218004449513579 2316 2 -0.5433889090632604 2316 3 -0.5293296022119911 2316 4 -0.44814914986550036 2316 5 -0.32762437015466617 2316 6 -0.36843207280066537 2316 7 -0.383612508839569 2316 8 -0.33797414986788965 2316 9 -0.3400477068678782 2316 10-11 -0.4650189241500087 2316 12-13 -0.3611640022704634 2316 14-15 -0.47652935937053087 2316 16-17 -0.3094798981921656 2316 18-19 -0.5219092472028706 2316 20-21 -0.6145834758672422 2316 22-23 -0.5860493310599466 2316 24-25 -0.49838475585699626 2316 26-27 -0.37966611878947276 2316 28-29 -0.42041057244713187 2316 30-31 -0.45743817224232686 2316 32-33 -0.2421572146410469 2316 34-35 -0.2928783487679354 2316 36-37 -0.16623290464027463 2316 38-39 -0.2133603720810271 2316 40-41 -0.07988238779298484 2316 42-43 -0.02406023160275339 2316 44-45 0.09402085404999383 2316 46-47 0.2732718041458355 2316 48-49 0.47622626785710764 2316 50-51 0.3145077872797515 2316 52-53 0.2929837537193549 2316 54-55 0.5900059059706209 2316 56-57 0.8160072679157295 2316 58-59 0.9530187641005412 2316 60-61 0.976308482188216 2316 62-63 1.1613630067364014 2316 64-65 1.3638406017202556 2316 66-67 1.6420823951314745 2316 68-69 1.7575278865460362 2316 70-71 2.203184146050809 2316 72-73 2.6420457578058674 2316 74-75 2.8470216652411757 2316 76-77 3.1002793351000086 2316 78-79 3.3867038320099603 2316 80-81 3.690107960517228 2316 82-83 3.7186016324858464 2316 84-85 3.8969955260938676 2316 86-87 4.202686674777336 2316 88-89 4.418292578987778 2316 90-91 4.831170969087342 2316 92-93 5.001723610471707 2316 94-95 5.254620921744948 2316 96-97 5.392727605918029 2316 98-99 5.251276294343494 2316 100-101 4.947604136221942 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5204.0 3 1295.0 4 1287.0 5 1401.0 6 1728.0 7 2059.0 8 2684.0 9 3472.0 10 4583.0 11 6039.0 12 8301.0 13 10928.0 14 13250.0 15 16736.0 16 21003.0 17 27174.0 18 27842.0 19 24562.0 20 25474.0 21 28286.0 22 32385.0 23 36584.0 24 41306.0 25 46718.0 26 51440.0 27 57535.0 28 63328.0 29 69757.0 30 77009.0 31 86109.0 32 97994.0 33 113778.0 34 137221.0 35 170423.0 36 212641.0 37 267710.0 38 180093.0 39 13111.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.09719435083866 19.430944177299864 14.764706624764933 22.70715484709654 2 14.361197226706157 21.044779047476283 42.04368194345865 22.55034178235891 3 19.036811374722337 25.645752465957088 30.186987595374237 25.130448563946334 4 11.611889899099664 17.786752957180745 39.61243726430092 30.988919879418663 5 12.719181273719581 38.55416594122022 35.12667049769484 13.599982287365355 6 29.66958650006441 37.578497359268326 19.06710920391601 13.684806936751256 7 26.93502080921773 33.75937773069104 21.571761240071417 17.73384022001981 8 24.219727415822923 36.04614603722691 20.897717800472165 18.836408746478 9 25.800247611544673 17.39523923792793 21.417241551048374 35.38727159947902 10-11 23.548798628470013 27.36412098882563 28.60198918129258 20.485091201411773 12-13 25.39466025839991 25.11065981369812 28.26542659930407 21.229253328597895 14-15 22.047758805031368 26.713099327278073 27.708059760504256 23.531082107186304 16-17 20.42152449674423 29.678558205423332 28.743882856705916 21.15603444112652 18-19 19.91266307449419 28.95748273269704 30.922513253084173 20.207340939724595 20-21 21.8000688742828 27.823550024035566 30.342567803310345 20.033813298371292 22-23 20.645134191473296 28.383333928588495 29.75693971104805 21.214592168890153 24-25 20.406976261521002 28.023561121533135 30.81617389383659 20.753288723109275 26-27 20.90128139982995 28.087610116367408 30.418806944814786 20.592301538987858 28-29 20.19402124987455 28.310888768545095 30.774286550088576 20.72080343149178 30-31 20.662704579676838 28.673906623473783 30.663771153504626 19.999617643344756 32-33 20.21035568992445 28.501402480268307 30.378318769389278 20.90992306041796 34-35 20.7001773610259 28.39185039794924 30.321138913854256 20.5868333271706 36-37 20.895885056256933 28.25031253851707 30.307105251904787 20.546697153321208 38-39 20.91190330144134 28.152273772688872 30.58231245502967 20.353510470840114 40-41 21.074084617837567 27.922613880190283 30.78192885373904 20.221372648233114 42-43 20.376616472261635 28.436080219172787 30.518588896606637 20.66871441195894 44-45 20.806962025316455 28.73364408893753 29.898726130026475 20.56066775571954 46-47 20.54941522997467 28.39788806779429 29.33185522892846 21.720841473302578 48-49 20.90319082559994 28.208498758540767 29.780787447040254 21.10752296881904 50-51 21.43439309447319 28.724929429589906 29.17248099785617 20.668196478080738 52-53 20.83019947791289 28.71808528160441 29.756702175731775 20.695013064750924 54-55 20.752071870266985 28.460206285530216 29.814462719411296 20.973259124791504 56-57 21.0758239492227 29.06120334558842 28.90586886218811 20.95710384300077 58-59 20.8445369033211 28.78233486715267 29.1532628257564 21.21986540376983 60-61 21.051060566054343 28.2822979140734 29.30456540052557 21.36207611934669 62-63 21.569583023400963 28.05190971148251 29.359283662678916 21.019223602437613 64-65 21.30690934036918 28.147949951063577 29.435787665204444 21.109353043362802 66-67 20.723387583881646 28.38585633336301 29.626145475156246 21.2646106075991 68-69 21.015153675484875 28.65699573435892 29.295069051683836 21.03278153847237 70-71 20.91561950246654 28.208509851705703 29.087737961855687 21.78813268397207 72-73 20.821134060804813 28.02259872797278 29.09049299228183 22.065774218940582 74-75 21.196645377334693 27.952288492117283 29.000476048809965 21.85059008173806 76-77 21.190693533907194 27.686535448713595 29.320447921460346 21.802323095918865 78-79 20.896252771808204 27.671183203131484 29.475970453492717 21.9565935715676 80-81 20.7947196731255 28.382586810951743 29.000304785126485 21.822388730796273 82-83 21.007625049069546 28.219119381144065 28.923155770943232 21.850099798843154 84-85 21.259313854549596 27.61349317292041 28.925649345179227 22.201543627350766 86-87 21.20871820429815 27.637863306707732 29.13016605504726 22.023252433946862 88-89 21.861681725865985 27.12537176415848 29.209212834587746 21.8037336753878 90-91 21.277729112011993 27.549954088188404 29.291157342331093 21.88115945746851 92-93 20.988108237521395 27.700166433371574 29.296397766201604 22.015327562905426 94-95 20.971319529033725 27.969564711081308 28.7897540966083 22.26936166327667 96-97 21.05234433866884 27.47051657238744 28.650557878863175 22.82658121008055 98-99 21.40168105352929 27.09792799501634 28.635779364050624 22.864611587403743 100-101 22.27035553143436 26.34853539254058 28.888955457124073 22.492153618900993 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2410.0 1 1403.5 2 543.0 3 738.0 4 565.5 5 482.0 6 1218.5 7 2462.5 8 3322.0 9 3354.0 10 2650.5 11 2020.0 12 1934.5 13 1945.5 14 1966.5 15 1984.5 16 2091.0 17 2333.5 18 2664.5 19 3157.5 20 3762.0 21 4550.0 22 5578.0 23 6921.5 24 8672.5 25 10715.5 26 13222.5 27 16439.0 28 20004.0 29 24228.0 30 28728.0 31 33309.5 32 38385.0 33 43922.0 34 49936.0 35 55805.5 36 60648.0 37 64305.0 38 67226.0 39 69168.0 40 70205.5 41 71614.0 42 73339.0 43 74521.0 44 75976.0 45 77845.5 46 79822.0 47 82221.0 48 94262.0 49 94933.0 50 80029.0 51 71070.0 52 63140.0 53 56149.0 54 49753.0 55 43194.0 56 36886.0 57 30508.0 58 24603.5 59 19587.5 60 15029.5 61 11195.5 62 8182.0 63 5857.0 64 4105.0 65 2896.5 66 2042.0 67 1516.5 68 1138.5 69 834.0 70 608.0 71 446.0 72 336.0 73 257.5 74 207.5 75 174.0 76 124.0 77 80.5 78 57.0 79 43.5 80 32.0 81 19.5 82 13.0 83 8.0 84 4.5 85 3.5 86 2.5 87 2.0 88 3.0 89 2.0 90 0.5 91 0.0 92 0.0 93 0.5 94 0.5 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.3835146050870753 2 0.024642296943701406 3 0.04455729610636621 4 0.03756693023866316 5 0.05878947985140192 6 0.056476121362809543 7 0.031682953213330374 8 0.029570756332441682 9 0.03319166527110801 10-11 0.030073660351700896 12-13 0.030274821959404584 14-15 0.024265118929256993 16-17 0.029922789145923136 18-19 0.026025282996664238 20-21 0.03879904508584823 22-23 0.025547524178367986 24-25 0.047247832609403 26-27 0.03927680390414448 28-29 0.03155722720851557 30-31 0.039125932698366714 32-33 0.03633481539147809 34-35 0.03507755534333006 36-37 0.03500211974044118 38-39 0.016822139444220653 40-41 0.013326956510369126 42-43 0.012472019677628465 44-45 0.0090019819447399 46-47 0.016042638214368875 48-49 0.013402392113258008 50-51 0.009479740763036152 52-53 0.004299829364666266 54-55 0.004551281374295872 56-57 0.008096754710073317 58-59 0.0029922789145923135 60-61 0.006336590642666075 62-63 0.005733105819555021 64-65 0.007895593102369633 66-67 0.004224393761777383 68-69 0.00611028383399943 70-71 0.017274753061553943 72-73 0.014383054950813472 74-75 0.010535839203480498 76-77 0.007845302700443712 78-79 0.006437171446517918 80-81 0.008297916317777003 82-83 0.010133515988073128 84-85 0.004148958158888502 86-87 7.040656269628972E-4 88-89 0.02330960129266449 90-91 0.020141305971331454 92-93 0.013477827716146891 94-95 0.02491889415429397 96-97 0.019361804741479676 98-99 0.017727366678887232 100-101 0.10988452820813789 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1988451.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.06444901577665 #Duplication Level Percentage of deduplicated Percentage of total 1 77.35875564643383 51.88022323974184 2 14.12383982664904 18.944150719226002 3 4.651599750746526 9.358709229772192 4 1.859941016027467 4.989436777669034 5 0.8434107589094212 2.828143892011918 6 0.44347127395107644 1.784469398511209 7 0.23872161471028527 1.1206813491091256 8 0.14489618223788278 0.7773906101018536 9 0.09472309100393388 0.5717296716525084 >10 0.2224709677296673 2.2322604920721614 >50 0.008461279882293256 0.41040960424487083 >100 0.007301191412827991 1.0180385207353129 >500 8.27543855101946E-4 0.40771416547037514 >1k 0.0011284688933208345 1.425359842680802 >5k 3.7615629777361154E-4 1.715921144369193 >10k+ 7.52312595547223E-5 0.5353613426316234 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10611 0.5336314548359502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8317 0.4182652728178869 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8295 0.4171588839755166 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6531 0.3284466149781916 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5753 0.2893206822798249 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5114 0.2571851154491612 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3039 0.15283253145287462 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2706 0.13608582761154286 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2689 0.1352308907788022 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2681 0.1348285675633948 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2341 0.11772983090858159 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2088 0.10500635922132354 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.5145200962960615E-5 0.0 18-19 0.0 0.0 0.0 1.2572600481480307E-4 0.0 20-21 0.0 0.0 0.0 2.765972105925668E-4 0.0 22-23 0.0 0.0 0.0 6.286300240740154E-4 0.0 24-25 0.0 0.0 0.0 0.001181824445259149 0.0 26-27 0.0 0.0 0.0 0.0028665529097775103 0.0 28-29 0.0 0.0 0.0 0.006663478255184563 0.0 30-31 0.0 0.0 0.0 0.018607448712590853 0.0 32-33 0.0 0.0 0.0 0.04121298437829245 0.0 34-35 0.0 0.0 0.0 0.0821745167469553 0.0 36-37 0.0 0.0 0.0 0.1444340343312458 0.0 38-39 0.0 0.0 0.0 0.2323416568977561 0.0 40-41 0.0 0.0 0.0 0.3251023032501178 0.0 42-43 0.0 0.0 0.0 0.41517241309944275 0.0 44-45 0.0 0.0 0.0 0.5237745360584697 0.0 46-47 0.0 0.0 0.0 0.651889334964754 0.0 48-49 2.5145200962960615E-5 0.0 0.0 0.7905651182754818 0.0 50-51 5.029040192592123E-5 0.0 0.0 0.9260726062648765 0.0 52-53 5.029040192592123E-5 0.0 0.0 1.058638105741605 0.0 54-55 5.029040192592123E-5 0.0 0.0 1.2024938004507026 0.0 56-57 5.029040192592123E-5 0.0 0.0 1.3634733770155765 0.0 58-59 5.029040192592123E-5 0.0 0.0 1.5485672013039296 0.0 60-61 5.029040192592123E-5 0.0 0.0 1.7518158606875402 0.0 62-63 7.543560288888184E-5 0.0 0.0 1.9375886054018934 0.0 64-65 1.0058080385184246E-4 0.0 0.0 2.1267308070452833 0.0 66-67 1.0058080385184246E-4 0.0 0.0 2.334405021798375 0.0 68-69 1.0058080385184246E-4 0.0 0.0 2.563528092972872 0.0 70-71 1.0058080385184246E-4 0.0 0.0 2.805575797442331 0.0 72-73 1.0058080385184246E-4 0.0 0.0 3.0521496380851225 0.0 74-75 1.0058080385184246E-4 5.029040192592123E-5 0.0 3.297491363880729 0.0 76-77 1.0058080385184246E-4 5.029040192592123E-5 0.0 3.5530420412672976 0.0 78-79 1.0058080385184246E-4 5.029040192592123E-5 0.0 3.833159579994679 0.0 80-81 1.0058080385184246E-4 5.029040192592123E-5 0.0 4.124869056365986 0.0 82-83 1.0058080385184246E-4 5.029040192592123E-5 0.0 4.424901594256031 0.0 84-85 1.0058080385184246E-4 5.029040192592123E-5 0.0 4.749375267482075 0.0 86-87 1.0058080385184246E-4 5.029040192592123E-5 0.0 5.086672993199229 0.0 88-89 1.0058080385184246E-4 5.029040192592123E-5 0.0 5.41851421030742 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GTATAGC 1630 0.0 26.056059 1 GAGTACT 7225 0.0 23.406487 12-13 TAGCCCT 2485 0.0 23.33271 4 TAGGACG 755 0.0 22.661428 4 ATAGCCC 1535 0.0 21.982733 3 GTCCTAC 935 0.0 21.946392 1 GTGTAGC 1645 0.0 21.757132 1 TATAGCC 1760 0.0 21.332718 2 AGTACTT 7465 0.0 21.28582 12-13 GTACTTT 8030 0.0 21.208435 14-15 GTACATG 8940 0.0 20.977865 1 AGGACGT 795 0.0 20.325607 5 GGACGTG 825 0.0 20.159016 6 GTATCAA 18295 0.0 19.980331 1 TACATGG 9085 0.0 19.774185 2 GACGACC 380 0.0 19.390263 94-95 GCGCCGT 100 1.2773302E-5 19.014967 94-95 GTAGCCC 1455 0.0 18.945093 3 TCGAGAC 280 3.470268E-8 18.67095 3 GTAGGAC 1080 0.0 18.482353 3 >>END_MODULE