FastQCFastQC Report
Thu 26 May 2016
SRR938115_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938115_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1988451
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT98240.4940529085202502No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83190.4183658536217387No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA78360.3940755894915188No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA67090.33739830652100555No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57510.289220101475973No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT53210.26759522864782687No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG31900.16042638214368873No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT28230.14196980463687564No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA27870.14015935016754247No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27200.13678989323850577No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24080.12109928783761834No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG21250.10686710409258261No Hit

[WARN]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTACT650.00581131129.2654554
GTATAGC14100.027.6659471
ATACCGT1401.782019E-523.7470876
TAGGACG7100.023.4432754
TAGCCCT24000.023.3818784
GAGTACT72750.022.42508512-13
GTACATG86450.022.3965031
GTGTAGC16100.022.1608371
GTAGCCC14350.021.541753
TATAGCC14700.021.3588282
GTAGGAC10700.021.334233
TACATGG88400.021.149062
TATACCG1150.003699798620.6756385
GATTCGC1150.00372686520.6496437
GTACTTT79200.020.53883414-15
ATAGCCC13550.020.3567393
AGTACTT74650.020.10470212-13
ACATGGG92650.019.4541993
AGGACGT8350.019.3632935
TAAGGCG1300.007515640518.2899885
Produced by FastQC (version 0.11.5)