##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938115_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1988451 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.42987229758239 33.0 31.0 34.0 31.0 34.0 2 32.656587967216694 34.0 31.0 34.0 31.0 34.0 3 32.732186008103795 34.0 31.0 34.0 31.0 34.0 4 36.1883461045809 37.0 35.0 37.0 35.0 37.0 5 36.123069665785074 37.0 35.0 37.0 35.0 37.0 6 36.173395270992344 37.0 37.0 37.0 35.0 37.0 7 36.150885287090304 37.0 36.0 37.0 35.0 37.0 8 36.14838937444272 37.0 36.0 37.0 35.0 37.0 9 37.83302077848536 39.0 38.0 39.0 35.0 39.0 10-11 37.80235243413089 39.0 38.0 39.0 35.0 39.0 12-13 37.72704331160285 39.0 37.5 39.0 35.0 39.0 14-15 38.935501302269955 40.0 38.0 41.0 35.5 41.0 16-17 38.84469041480027 40.0 38.0 41.0 35.0 41.0 18-19 39.12224264012541 40.0 38.5 41.0 36.0 41.0 20-21 39.108984078561654 40.0 39.0 41.0 36.0 41.0 22-23 39.00155347051549 40.0 38.5 41.0 35.0 41.0 24-25 38.90797912545997 40.0 38.0 41.0 35.0 41.0 26-27 38.73118221168136 40.0 38.0 41.0 34.5 41.0 28-29 38.606914377070396 40.0 38.0 41.0 34.5 41.0 30-31 38.402164549189294 40.0 38.0 41.0 34.0 41.0 32-33 38.194935153041236 40.0 38.0 41.0 34.0 41.0 34-35 38.12526484182914 40.0 38.0 41.0 34.0 41.0 36-37 38.082589664014854 40.0 38.0 41.0 34.0 41.0 38-39 37.900097110766126 40.0 37.5 41.0 33.0 41.0 40-41 37.697992809478336 40.0 37.0 41.0 33.0 41.0 42-43 37.48463753947168 40.0 37.0 41.0 33.0 41.0 44-45 37.22346112627367 40.0 37.0 41.0 32.0 41.0 46-47 36.86178336805885 40.0 36.5 41.0 31.0 41.0 48-49 36.479494340066715 39.0 36.0 41.0 30.0 41.0 50-51 36.13508228264111 39.0 35.5 40.5 29.5 41.0 52-53 35.611447302447985 39.0 35.0 40.0 28.0 41.0 54-55 35.280304619022544 38.0 35.0 40.0 27.0 41.0 56-57 34.96260078825176 38.0 34.0 40.0 26.0 41.0 58-59 34.51680755522766 38.0 34.0 40.0 25.5 41.0 60-61 34.08558093712141 38.0 33.0 40.0 23.5 41.0 62-63 33.54426209144706 37.0 32.5 40.0 22.5 41.0 64-65 32.97985768822063 36.5 32.0 39.0 20.5 40.0 66-67 32.43785841340822 36.0 31.0 39.0 18.5 40.0 68-69 31.84173333916702 35.5 31.0 39.0 13.5 40.0 70-71 31.22742878753361 35.0 30.0 38.0 8.0 40.0 72-73 30.526140448017074 34.5 29.0 37.5 7.5 39.0 74-75 29.84074261824908 34.0 28.5 37.0 4.0 39.0 76-77 28.457525732341406 32.5 27.0 35.0 2.0 37.5 78-79 29.104318637974988 34.0 28.0 36.0 2.0 38.0 80-81 28.80867896669317 34.0 28.0 35.5 2.0 37.0 82-83 28.218768025965943 33.5 26.5 35.0 2.0 37.0 84-85 27.513865315262986 33.0 26.0 35.0 2.0 36.0 86-87 26.73295042221307 32.5 25.5 35.0 2.0 36.0 88-89 25.881635504219112 32.0 23.0 34.5 2.0 35.0 90-91 25.17016008943645 31.5 19.5 34.0 2.0 35.0 92-93 24.215148122835313 31.0 12.0 34.0 2.0 35.0 94-95 23.27780418023879 31.0 2.0 34.0 2.0 35.0 96-97 22.167934236247213 30.0 2.0 34.0 2.0 35.0 98-99 20.717976957943645 29.0 2.0 34.0 2.0 35.0 100-101 16.893270440156684 22.0 2.0 31.5 2.0 33.5 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.10500951307200523 1101 2 0.17693171209807446 1101 3 0.1617940654146679 1101 4 5.811006924005824E-4 1101 5 -0.021799690048439402 1101 6 0.06593767917345161 1101 7 0.0926864972147996 1101 8 0.16507400090487323 1101 9 -0.03572374967111358 1101 10-11 0.11339890371045414 1101 12-13 0.15071167055133827 1101 14-15 0.30294216107147065 1101 16-17 0.3156742642007373 1101 18-19 0.2333404222857851 1101 20-21 0.19228191975668096 1101 22-23 0.22235719959917333 1101 24-25 0.2509411375315054 1101 26-27 0.407809687137501 1101 28-29 0.417165652280147 1101 30-31 0.4308906377056161 1101 32-33 0.39537107256403203 1101 34-35 0.30176795330896766 1101 36-37 0.3397461694992927 1101 38-39 0.3202010515808169 1101 40-41 0.5617030778429211 1101 42-43 0.6321567720508128 1101 44-45 0.5841205456899985 1101 46-47 0.6558020852836322 1101 48-49 0.6505362080333015 1101 50-51 0.6513052857400154 1101 52-53 0.6988892991471971 1101 54-55 0.7695876335028657 1101 56-57 0.8186701166330721 1101 58-59 1.2294437424560911 1101 60-61 1.0077691674216638 1101 62-63 1.1077715561971075 1101 64-65 1.3266173680724833 1101 66-67 1.4996198671425418 1101 68-69 1.8069975343862659 1101 70-71 1.9171926906397374 1101 72-73 1.9590123740499727 1101 74-75 1.9541916832923931 1101 76-77 1.8686726096173096 1101 78-79 2.099644977957947 1101 80-81 2.0999907942293916 1101 82-83 2.3713390708427333 1101 84-85 2.441188845914091 1101 86-87 2.6161093901828245 1101 88-89 2.965922076575797 1101 90-91 3.228321127181683 1101 92-93 3.405162927635846 1101 94-95 3.5913440956005154 1101 96-97 3.9210163952150943 1101 98-99 3.88372308098743 1101 100-101 4.020036103558507 1102 1 0.2048765477882597 1102 2 0.15750393656621497 1102 3 0.19226174948157393 1102 4 0.15244324632886475 1102 5 -0.020125780300595864 1102 6 0.07279628749120093 1102 7 0.06379734162330664 1102 8 0.09141091787895306 1102 9 0.22419874514924487 1102 10-11 0.2441393087334447 1102 12-13 0.24847510604580236 1102 14-15 0.3579946839377115 1102 16-17 0.40406159428310673 1102 18-19 0.43356687384979153 1102 20-21 0.3227565521281974 1102 22-23 0.38281297679862547 1102 24-25 0.35688130631214676 1102 26-27 0.3153793912660632 1102 28-29 0.2680116083668622 1102 30-31 0.4532273841303933 1102 32-33 0.40544532467379213 1102 34-35 0.387287844410082 1102 36-37 0.44624030765685774 1102 38-39 0.43498972837094385 1102 40-41 0.6423770002240374 1102 42-43 0.7035940288601523 1102 44-45 0.6586084240567729 1102 46-47 0.6484242491120185 1102 48-49 0.7824464548876193 1102 50-51 0.8872455492702542 1102 52-53 0.9539776689486033 1102 54-55 1.0803767714077566 1102 56-57 1.2455690567007878 1102 58-59 1.4313453670960428 1102 60-61 1.149280707923971 1102 62-63 1.408258095436075 1102 64-65 1.417745014744881 1102 66-67 1.6887230305161296 1102 68-69 1.9321335000742756 1102 70-71 2.029783966095792 1102 72-73 2.1352513830954045 1102 74-75 2.1978101392527734 1102 76-77 2.2864883784788006 1102 78-79 2.524765309960575 1102 80-81 2.6116099387035945 1102 82-83 2.6718901977314076 1102 84-85 2.9050368170091474 1102 86-87 2.9769937514441374 1102 88-89 3.2514568117822265 1102 90-91 3.5347162519985 1102 92-93 3.702826244377473 1102 94-95 3.846825992108677 1102 96-97 4.136798205816941 1102 98-99 4.523222139807988 1102 100-101 4.26228432226587 1103 1 0.3064722523723944 1103 2 0.24604755353337993 1103 3 0.18289509778913526 1103 4 0.11645761667917043 1103 5 0.04792317253550493 1103 6 0.13684326201398278 1103 7 0.1413045687793968 1103 8 0.17922216668617352 1103 9 0.2804415555308495 1103 10-11 0.25421120951379805 1103 12-13 0.25686363256396305 1103 14-15 0.4259898582750381 1103 16-17 0.4451229336404481 1103 18-19 0.36603624768420673 1103 20-21 0.3538046720870369 1103 22-23 0.49775279697313124 1103 24-25 0.3639109681419157 1103 26-27 0.48157027398725916 1103 28-29 0.481646771354022 1103 30-31 0.40242861254775875 1103 32-33 0.5416998161743365 1103 34-35 0.6380755232197615 1103 36-37 0.6257903210478517 1103 38-39 0.6599765978372005 1103 40-41 0.7856613067694482 1103 42-43 0.7972098315877503 1103 44-45 0.8939359335821564 1103 46-47 0.9303585651874116 1103 48-49 1.1312001726519298 1103 50-51 1.1495546968856516 1103 52-53 1.1847586737086928 1103 54-55 1.179072573865696 1103 56-57 1.32373081874794 1103 58-59 1.4746934417645008 1103 60-61 1.5355414812434631 1103 62-63 1.5490129739579501 1103 64-65 1.6555714624896467 1103 66-67 1.8519665144313109 1103 68-69 1.946177961869484 1103 70-71 2.007364460710896 1103 72-73 2.1871088709929403 1103 74-75 2.1139895308417564 1103 76-77 2.2382006491081405 1103 78-79 2.233019865198596 1103 80-81 2.41918556720724 1103 82-83 2.51954306502936 1103 84-85 2.6939589671930335 1103 86-87 2.8631052932599026 1103 88-89 3.057588847649267 1103 90-91 3.1194748432285913 1103 92-93 3.4431975948704867 1103 94-95 3.608434027443753 1103 96-97 3.950146519500951 1103 98-99 4.575024010625597 1103 100-101 4.355863506585468 1104 1 0.2416156257588966 1104 2 0.2030130903971923 1104 3 0.2304361980044476 1104 4 0.16599622496526933 1104 5 -0.013548884798687766 1104 6 0.05406445192475928 1104 7 0.11252027924450658 1104 8 0.19730352112024718 1104 9 0.20252591380660334 1104 10-11 0.22299985438589687 1104 12-13 0.28804656430644826 1104 14-15 0.5232186802291992 1104 16-17 0.366449918015455 1104 18-19 0.3613105318959029 1104 20-21 0.3613801602258917 1104 22-23 0.4090980714322541 1104 24-25 0.43194445467972287 1104 26-27 0.4080204642636076 1104 28-29 0.39567404730922817 1104 30-31 0.43649178067951055 1104 32-33 0.4849002718813722 1104 34-35 0.4695178511504139 1104 36-37 0.6099717596151564 1104 38-39 0.4606589923048148 1104 40-41 0.48651348359263835 1104 42-43 0.5700616504220406 1104 44-45 0.5044514492523291 1104 46-47 0.658205815873508 1104 48-49 0.8663988768953743 1104 50-51 1.0133075632747435 1104 52-53 1.0116924696678424 1104 54-55 1.0274469311327792 1104 56-57 1.0108875881488686 1104 58-59 1.2419314233368226 1104 60-61 1.1638510565135363 1104 62-63 1.30779273907293 1104 64-65 1.5011005659956638 1104 66-67 1.6331573172651304 1104 68-69 1.8254475187620152 1104 70-71 1.7896624637275664 1104 72-73 2.0149937571565566 1104 74-75 2.0629906893497925 1104 76-77 2.005362251942202 1104 78-79 2.082589608023806 1104 80-81 2.1300016711103105 1104 82-83 2.0923059113792455 1104 84-85 2.4223694381933107 1104 86-87 2.619753595990243 1104 88-89 2.8625536988535742 1104 90-91 3.20844062263863 1104 92-93 3.440146672407735 1104 94-95 3.657516695475522 1104 96-97 3.7488050231656693 1104 98-99 4.0384384492389565 1104 100-101 3.7644786784347133 1105 1 0.2168940812065756 1105 2 0.21302247943884112 1105 3 0.15037372899433166 1105 4 0.10931290942420446 1105 5 0.003786210537462864 1105 6 0.012077350313120405 1105 7 0.09687993890823776 1105 8 0.05922798880886404 1105 9 0.09575305055568606 1105 10-11 0.08531406625061067 1105 12-13 0.15585897257363257 1105 14-15 0.3245714608257728 1105 16-17 0.2634919026433735 1105 18-19 0.21691512461048745 1105 20-21 0.18624100121576248 1105 22-23 0.22793093329790537 1105 24-25 0.19970791129828314 1105 26-27 0.251148021100839 1105 28-29 0.2712781782676785 1105 30-31 0.34248166492164245 1105 32-33 0.49547325079121407 1105 34-35 0.504356665272681 1105 36-37 0.46618020843333596 1105 38-39 0.5117663275210873 1105 40-41 0.449019983909821 1105 42-43 0.515960046479087 1105 44-45 0.5959962544407134 1105 46-47 0.6319171863987307 1105 48-49 0.6661056892277841 1105 50-51 0.6785171785769037 1105 52-53 0.8508797073876053 1105 54-55 0.9537057113709437 1105 56-57 0.8939813763193385 1105 58-59 0.9823389891013363 1105 60-61 1.014219389496887 1105 62-63 1.0359839687845138 1105 64-65 1.1836718001269233 1105 66-67 1.3057694607921349 1105 68-69 1.3771987014624436 1105 70-71 1.5803341100311528 1105 72-73 1.6637054849930841 1105 74-75 1.5713782973540056 1105 76-77 1.4766566863513901 1105 78-79 1.6003619974249652 1105 80-81 1.7440691820577818 1105 82-83 1.8803201235737852 1105 84-85 2.051453918679165 1105 86-87 2.244692715641154 1105 88-89 2.2461012723799954 1105 90-91 2.327474760710313 1105 92-93 2.6692747448726664 1105 94-95 3.0470034700098907 1105 96-97 3.4463695893182873 1105 98-99 3.6214055842949335 1105 100-101 3.386559445081847 1106 1 0.1615370581076121 1106 2 0.07142066848219031 1106 3 0.09055052400984209 1106 4 0.1559318431399177 1106 5 0.07664442855696763 1106 6 0.08446744689676677 1106 7 0.12681522690804314 1106 8 0.10416392084156456 1106 9 0.12895268052950826 1106 10-11 0.13815350618682487 1106 12-13 0.1369675181983112 1106 14-15 0.17324847885762296 1106 16-17 0.030166100769186244 1106 18-19 0.015352652886726048 1106 20-21 0.02855885966265248 1106 22-23 -0.07396707424526738 1106 24-25 -0.06458043766748034 1106 26-27 -0.07303432324279413 1106 28-29 0.12400288986334829 1106 30-31 0.11503173182655502 1106 32-33 0.16692372466103933 1106 34-35 0.11342004449734588 1106 36-37 0.13150651488867027 1106 38-39 0.007098685756666612 1106 40-41 0.13146492441927649 1106 42-43 0.07652921188695672 1106 44-45 0.11391314332534108 1106 46-47 0.2268349950262163 1106 48-49 0.19270225211467107 1106 50-51 0.2413719954639788 1106 52-53 0.3316038292407555 1106 54-55 0.4430244789155111 1106 56-57 0.4835945627067062 1106 58-59 0.6593892226031812 1106 60-61 0.6222714773876916 1106 62-63 0.7126008107239414 1106 64-65 0.7881578884839726 1106 66-67 0.9575930319705463 1106 68-69 0.9175714474803449 1106 70-71 0.8284006806783708 1106 72-73 1.1667653066012953 1106 74-75 1.5581347720137089 1106 76-77 1.5872916921476836 1106 78-79 1.628013629915305 1106 80-81 1.4578327506600566 1106 82-83 1.8425136623802274 1106 84-85 1.9075162908705678 1106 86-87 2.16496357139588 1106 88-89 2.2647806217045066 1106 90-91 2.07210560211535 1106 92-93 2.364617641828467 1106 94-95 2.741305402215051 1106 96-97 3.201970812661447 1106 98-99 3.356868271370523 1106 100-101 3.4339657223268354 1107 1 0.2709524418418354 1107 2 0.19481075498682543 1107 3 0.21602056134236136 1107 4 0.16360544041275205 1107 5 0.07012747910555817 1107 6 0.10925131789102238 1107 7 0.18760022538202037 1107 8 0.16668203489574296 1107 9 0.1752164720698559 1107 10-11 0.22450169430411648 1107 12-13 0.2968478282181124 1107 14-15 0.44992661919490473 1107 16-17 0.3943103630190592 1107 18-19 0.3634970180204533 1107 20-21 0.3687054638459415 1107 22-23 0.3735987548460997 1107 24-25 0.37558451590352604 1107 26-27 0.4859844140806828 1107 28-29 0.4282916880129264 1107 30-31 0.44405547810861634 1107 32-33 0.5731603421370295 1107 34-35 0.5970102029676454 1107 36-37 0.658302852329669 1107 38-39 0.6357719110036228 1107 40-41 0.731644759025535 1107 42-43 0.7020444597773761 1107 44-45 0.7736680305070962 1107 46-47 0.8830041944122016 1107 48-49 1.0455874944604915 1107 50-51 1.0794223411988 1107 52-53 1.0938033215566847 1107 54-55 1.1823968821258077 1107 56-57 1.3143478448473758 1107 58-59 1.3739522990212265 1107 60-61 1.5792951960926374 1107 62-63 1.5622706675854587 1107 64-65 1.7510288835378631 1107 66-67 1.9399340001871224 1107 68-69 2.0399504615083437 1107 70-71 1.993436452703662 1107 72-73 2.1371216320886752 1107 74-75 2.177227469036943 1107 76-77 2.1114357210527466 1107 78-79 2.1598813721955032 1107 80-81 2.339363057776307 1107 82-83 2.5401202605230644 1107 84-85 2.6684868780218096 1107 86-87 2.862998808053927 1107 88-89 2.987317461680078 1107 90-91 3.2398008421197737 1107 92-93 3.4654859762211316 1107 94-95 3.639421222383625 1107 96-97 4.0082336283962015 1107 98-99 4.066476217609999 1107 100-101 3.9519908137887185 1108 1 0.13596691644707448 1108 2 0.11593212037243461 1108 3 0.06790457167488029 1108 4 0.017783060409328755 1108 5 -5.013775824593836E-4 1108 6 0.03160180546631608 1108 7 0.10864322013259908 1108 8 0.0715961838117849 1108 9 0.05791057259344967 1108 10-11 0.04335093147310687 1108 12-13 0.11594880990897849 1108 14-15 0.1849968017900281 1108 16-17 0.15791535021053704 1108 18-19 0.20518650285193019 1108 20-21 0.2082114243837907 1108 22-23 0.13501880820015089 1108 24-25 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0.0626220903949175 2316 58-59 0.10315568337108516 2316 60-61 0.1981814987618975 2316 62-63 0.19416973585172315 2316 64-65 0.35816313731838534 2316 66-67 0.5573462547748136 2316 68-69 0.6954840465219085 2316 70-71 0.8855601329118699 2316 72-73 1.0937275209757438 2316 74-75 1.492764738050269 2316 76-77 1.410573252786346 2316 78-79 1.5756139111234368 2316 80-81 1.5917744081706218 2316 82-83 1.9038203363318935 2316 84-85 2.0836217753818573 2316 86-87 2.1802980776852863 2316 88-89 2.301496872020511 2316 90-91 2.6059283466065644 2316 92-93 2.655104290092087 2316 94-95 3.0967660400558863 2316 96-97 3.371931239241686 2316 98-99 3.5683950795319426 2316 100-101 3.8337786785948964 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 7.0 3 8.0 4 24.0 5 46.0 6 82.0 7 172.0 8 469.0 9 820.0 10 1422.0 11 2372.0 12 3889.0 13 5923.0 14 7691.0 15 10709.0 16 14704.0 17 21098.0 18 21765.0 19 17275.0 20 16123.0 21 16989.0 22 19077.0 23 21546.0 24 24673.0 25 27956.0 26 32116.0 27 37473.0 28 44860.0 29 53842.0 30 64796.0 31 77619.0 32 93361.0 33 115877.0 34 150330.0 35 201258.0 36 275953.0 37 372266.0 38 224700.0 39 9160.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.15396808872836 19.919324137230436 15.161852115038288 22.76485565900291 2 14.494004506679541 21.417038870111057 41.4306695638178 22.6582870593916 3 18.988907178273216 25.582490068663144 30.458061067097564 24.970541685966076 4 11.81775159713278 18.106001437331493 39.26107683185585 30.815170133679874 5 12.692541040765104 38.5701374780667 35.24508656259519 13.492234918573006 6 29.654705926726848 37.78349971083004 18.873645301616836 13.688149060826271 7 26.81313243323572 33.752453542983964 21.456148529684665 17.978265494095655 8 24.23933061295102 36.23885184712082 20.80926512462496 18.712552415303207 9 25.496479420413177 17.31106273174446 21.376790275445558 35.815667572396805 10-11 23.509807382731584 27.405427641918255 28.56892123567541 20.515843739674754 12-13 25.242009986668013 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20.583039824425224 38-39 20.853085097528094 28.110376349534494 30.612533316367625 20.424005236569787 40-41 21.06329548117904 27.966887663698248 30.717480895374955 20.25233595974776 42-43 20.353037413788204 28.430611496146668 30.562032887158136 20.65431820290699 44-45 20.778537916023136 28.792671022304077 29.88816026707155 20.540630794601235 46-47 20.5232112835569 28.468013544211047 29.24281262148275 21.7659625507493 48-49 20.848791345625315 28.20695606781359 29.779034032017886 21.165218554543213 50-51 21.39740934023519 28.651598656441625 29.270195745331417 20.680796257991773 52-53 20.821031043762204 28.686726502186875 29.714285139538266 20.777957314512655 54-55 20.7034520840594 28.470930387522753 29.772395698963667 21.053221829454184 56-57 20.969267276203254 29.06350950149375 28.913618920863254 21.053604301439737 58-59 20.755804392464285 28.8944510073419 29.141276299994317 21.208468300199502 60-61 20.96775329138108 28.457377124203713 29.198456486984092 21.376413097431115 62-63 21.522431279423028 28.250557846283364 29.157218357404837 21.069792516888775 64-65 21.248675476539276 28.324660753521208 29.25631559439986 21.170348175539655 66-67 20.60684498492166 28.596038569769345 29.471972746591124 21.32514369871787 68-69 20.880704213346046 28.795912758372616 29.19303481160905 21.13034821667229 70-71 20.76865881247735 28.434024382301697 28.879924343100317 21.91739246212063 72-73 20.702934446428124 28.216651160451605 28.832809474716488 22.24760491840378 74-75 21.037883256866778 28.14507372824374 28.734854416829986 22.082188598059492 76-77 20.975447722875746 27.825176481592955 29.330946550857927 21.868429244673365 78-79 20.6767730258377 27.751425607168596 29.53909852442932 22.03270284256439 80-81 20.493439985999135 28.56531109556018 29.042944788361382 21.898304130079303 82-83 20.66443054109327 28.416027986186947 29.00820982800575 21.91133164471403 84-85 20.932198658890048 27.884585885906578 28.902061153125224 22.28115430207815 86-87 20.803540557361178 27.946891639582326 29.189355696512877 22.06021210654362 88-89 21.384459561739263 27.481491874831214 29.102552690511356 22.031495872918168 90-91 20.834184101975563 27.766171561226678 29.4426216481811 21.957022688616664 92-93 20.46580478975846 28.041224048266717 29.364389668138667 22.12858149383616 94-95 20.43167815719689 28.371207911291663 28.835665385433572 22.361448546077874 96-97 20.420750253464007 27.95773387083796 28.6752532628462 22.946262612851832 98-99 20.73913312422584 27.573422729551794 28.83080850370464 22.85663564251772 100-101 21.25430800155498 27.304721112061596 28.948588625015148 22.49238226136827 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2302.0 1 1339.5 2 523.0 3 704.0 4 526.0 5 474.5 6 1274.0 7 2535.5 8 3305.0 9 3374.0 10 2697.5 11 1976.5 12 1837.5 13 1857.5 14 1918.0 15 1922.5 16 2053.0 17 2299.5 18 2596.5 19 3074.0 20 3658.0 21 4414.5 22 5413.5 23 6654.5 24 8326.0 25 10400.0 26 12936.5 27 16046.0 28 19575.0 29 23942.0 30 28534.0 31 33232.0 32 38442.5 33 43949.0 34 49879.5 35 55781.5 36 61049.5 37 64936.5 38 67706.0 39 69881.0 40 71050.5 41 72402.5 42 74259.5 43 75846.5 44 77313.5 45 78978.5 46 81152.0 47 83595.5 48 99224.5 49 98493.0 50 79292.0 51 70987.0 52 63132.5 53 55823.0 54 49462.5 55 42676.0 56 35612.5 57 29433.5 58 23428.0 59 18086.0 60 13520.0 61 9791.5 62 6935.5 63 4918.5 64 3368.5 65 2132.5 66 1443.0 67 1091.0 68 807.5 69 583.5 70 404.0 71 289.0 72 185.0 73 142.5 74 133.0 75 100.5 76 61.0 77 32.5 78 20.5 79 18.0 80 11.5 81 5.5 82 3.0 83 2.5 84 2.0 85 0.5 86 0.0 87 1.0 88 1.5 89 0.5 90 0.0 91 0.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.01463450696044308 3 0.002413939292444219 4 0.002212777684740534 5 0.11647257086043357 6 5.029040192592124E-5 7 0.0 8 2.514520096296062E-4 9 0.0 10-11 0.0 12-13 0.0 14-15 2.514520096296062E-5 16-17 2.514520096296062E-5 18-19 0.0 20-21 0.0 22-23 1.5087120577776368E-4 24-25 4.023232154073699E-4 26-27 0.004827878584888438 28-29 0.0 30-31 0.0 32-33 0.0 34-35 0.010737000811184182 36-37 5.280492202221729E-4 38-39 0.006361735843629036 40-41 0.009504885963999113 42-43 0.005355927805110611 44-45 0.0035203281348144865 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 2.514520096296062E-5 58-59 0.0 60-61 0.0 62-63 0.0 64-65 0.0 66-67 1.257260048148031E-4 68-69 9.80662837555464E-4 70-71 2.514520096296062E-5 72-73 5.029040192592124E-5 74-75 0.0 76-77 0.0 78-79 0.0 80-81 1.257260048148031E-4 82-83 0.002087051679925731 84-85 0.004928459388740281 86-87 0.003193440522295998 88-89 0.0 90-91 0.0026653913020738256 92-93 0.0 94-95 1.0058080385184247E-4 96-97 1.5087120577776368E-4 98-99 0.0 100-101 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1988451.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 66.05714014117883 #Duplication Level Percentage of deduplicated Percentage of total 1 76.65454256882663 50.6357986092694 2 14.509192104779277 19.168714724013807 3 4.736054289350767 9.385506057236242 4 1.9165495831688875 5.064071376116204 5 0.9012129512622898 2.976577510928923 6 0.48553427958900364 1.9243803570094273 7 0.2737746425474879 1.2659338950902386 8 0.16013729542564442 0.846256941260892 9 0.09314927330861772 0.5537857140896697 >10 0.24944910377217175 2.4370021955248338 >50 0.009283713628824736 0.43208370304910143 >100 0.008455642530311724 1.1326053087587353 >500 9.89690672284032E-4 0.45868902408234113 >1k 0.0012180808274264699 1.5179332861419943 >5k 4.5678031028492617E-4 2.2006612974282294 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 9824 0.4940529085202502 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8319 0.4183658536217387 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 7836 0.3940755894915188 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6709 0.33739830652100555 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5751 0.289220101475973 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5321 0.26759522864782687 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 3190 0.16042638214368873 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2823 0.14196980463687564 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2787 0.14015935016754247 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2720 0.13678989323850577 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2408 0.12109928783761834 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2125 0.10686710409258261 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 0.0 0.0 18-19 0.0 0.0 0.0 1.0058080385184246E-4 0.0 20-21 0.0 0.0 0.0 2.765972105925668E-4 0.0 22-23 0.0 0.0 0.0 6.286300240740154E-4 0.0 24-25 0.0 0.0 0.0 0.001181824445259149 0.0 26-27 0.0 0.0 0.0 0.0028414077088145494 0.0 28-29 0.0 0.0 0.0 0.006613187853258642 0.0 30-31 0.0 0.0 0.0 0.018557158310664935 0.0 32-33 0.0 0.0 0.0 0.04121298437829245 0.0 34-35 0.0 0.0 0.0 0.08252654956043673 0.0 36-37 0.0 0.0 0.0 0.14551527797265307 0.0 38-39 0.0 0.0 0.0 0.23385036895553374 0.0 40-41 0.0 0.0 0.0 0.32744080693967315 0.0 42-43 0.0 0.0 0.0 0.4183155632198128 0.0 44-45 0.0 0.0 0.0 0.5275463162029137 0.0 46-47 0.0 0.0 0.0 0.6571949723679387 0.0 48-49 0.0 0.0 0.0 0.7967759829133331 0.0 50-51 0.0 0.0 0.0 0.9326355037162093 0.0 52-53 0.0 0.0 0.0 1.0657793428150857 0.0 54-55 0.0 0.0 0.0 1.2099619251367018 0.0 56-57 0.0 0.0 0.0 1.3715952769266129 0.0 58-59 0.0 0.0 0.0 1.5570159888274842 0.0 60-61 0.0 0.0 0.0 1.760842987833243 0.0 62-63 0.0 0.0 0.0 1.9475461049832257 0.0 64-65 0.0 0.0 0.0 2.1371409202439486 0.0 66-67 0.0 0.0 0.0 2.3452426034134106 0.0 68-69 0.0 0.0 0.0 2.575648079837019 0.0 70-71 0.0 0.0 0.0 2.8216435808576623 0.0 72-73 0.0 0.0 0.0 3.0730955904872688 0.0 74-75 0.0 0.0 0.0 3.3235417920783563 0.0 76-77 0.0 0.0 0.0 3.58686233656248 0.0 78-79 0.0 0.0 0.0 3.8751017752008976 0.0 80-81 0.0 0.0 0.0 4.175712652713091 0.0 82-83 0.0 0.0 0.0 4.484973479356544 0.0 84-85 0.0 0.0 0.0 4.822447221480439 0.0 86-87 0.0 0.0 0.0 5.173599952928184 0.0 88-89 0.0 0.0 0.0 5.521886131466152 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCGTACT 65 0.005811311 29.265455 4 GTATAGC 1410 0.0 27.665947 1 ATACCGT 140 1.782019E-5 23.747087 6 TAGGACG 710 0.0 23.443275 4 TAGCCCT 2400 0.0 23.381878 4 GAGTACT 7275 0.0 22.425085 12-13 GTACATG 8645 0.0 22.396503 1 GTGTAGC 1610 0.0 22.160837 1 GTAGCCC 1435 0.0 21.54175 3 TATAGCC 1470 0.0 21.358828 2 GTAGGAC 1070 0.0 21.33423 3 TACATGG 8840 0.0 21.14906 2 TATACCG 115 0.0036997986 20.675638 5 GATTCGC 115 0.003726865 20.649643 7 GTACTTT 7920 0.0 20.538834 14-15 ATAGCCC 1355 0.0 20.356739 3 AGTACTT 7465 0.0 20.104702 12-13 ACATGGG 9265 0.0 19.454199 3 AGGACGT 835 0.0 19.363293 5 TAAGGCG 130 0.0075156405 18.289988 5 >>END_MODULE