FastQCFastQC Report
Thu 26 May 2016
SRR938114_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938114_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981673
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106700.5384339394037261No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT85100.4294351288027843No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA82920.41843432291805965No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA65470.330377413427947No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT57310.2892000849787023No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT50650.25559211837674534No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29810.15042845111176265No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27630.13942764522703796No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT27400.1382670097437872No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA26860.13554203947876367No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC21800.11000805884724674No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG20400.10294332112311164No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTGTAGC16550.028.838431
GTAGCCC14500.025.892983
TAGCCCT22650.024.7592094
GTATAGC13450.023.4202371
TAGGACG7200.023.1024744
CCGTCCA1050.00219648422.619579
TGTAGCC18850.021.4304122
GAGTACT73350.021.30587812-13
TATATCG1353.7837648E-421.1222635
GTACATG87050.021.0538791
AGGACGT7700.020.9851065
CAACCGT1150.003716642520.6594316
TACATGG88500.020.4599552
ATAGCCC12600.019.9907133
AGTACTT74400.019.95173812-13
GTACTTT78450.019.92104314-15
GTCCTAC10050.019.4709641
GACGTGG4900.019.3896717
CCGACGC2150.018.79943794-95
GGACGTG8600.018.785676
Produced by FastQC (version 0.11.5)