FastQCFastQC Report
Thu 26 May 2016
SRR938114_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938114_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1981673
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT102170.5155744666249175No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT84600.42691200818702174No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA81260.410057562473728No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA63390.31988123166637483No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT59310.2992925674417525No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT52750.266189224962948No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG31410.15850243708220277No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT28880.14573544676644432No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA28810.14538220988023756No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27110.1368035997866449No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC22880.11545799937729383No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC13700.025.6562751
GTGTAGC16600.022.604841
TAGCCCT25600.022.4494614
GAGTACT76250.021.36558312-13
GTAGCCC14950.021.2860033
TGTTACG1150.003724772520.6516422
CTATGCG1150.00372586220.6505993
TACCGTT1150.00372586220.6505997
TATAGCC16950.020.4567012
GTACATG89100.020.0443781
ATAGCCC15250.019.9329073
TACATGG88650.019.7710652
AGTACTT79800.019.76039912-13
GTACTTT83000.019.39912414-15
ACATGGG90650.018.705143
GTCCTAC8150.018.6498261
CACACCG2854.280446E-818.3319365
TGTAGCC20250.017.5921382
GTATCAA178850.017.2891851
GTACTAG4700.017.1804081
Produced by FastQC (version 0.11.5)