FastQCFastQC Report
Thu 26 May 2016
SRR938113_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938113_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994970
Sequences flagged as poor quality0
Sequence length101
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT108820.5454718617322566No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT87920.4407083815796729No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA84950.425820939663253No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA65000.32581943588124135No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60260.30205968009544004No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT53030.2658185336120342No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG29180.14626786367714803No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT27760.13914996215481937No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA27370.1371950455395319No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA27270.1366937848689454No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC22560.11308440728432007No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG21370.10711940530434043No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACCG1051.8954561E-631.6819345
GTATAGC15850.023.4963511
TAGCCCT23900.023.463194
GTACATG85750.022.828891
TACATGG87350.022.3061142
GAGTACT71950.022.20223812-13
ATAGCCC14050.021.9856833
GTGTAGC17600.021.4313491
ACATGGG88150.021.3489153
GTAGCCC14100.021.2336373
AGTACTT73250.020.86707712-13
TCGCCGC704.8253778E-420.37539594-95
GTACTTT79200.020.18692614-15
TATAGCC17700.019.5998422
GTCCTAC8100.019.4519881
CGCCGTA1503.6834535E-919.01703694-95
TAGGACG7700.018.5154154
CATGGGG32100.018.5058024
ACCGTCT1650.001453967817.2910398
TACTTTT89500.017.12052314-15
Produced by FastQC (version 0.11.5)