##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR938113_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1994970 Sequences flagged as poor quality 0 Sequence length 101 %GC 42 >>END_MODULE >>Per base sequence quality fail #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 32.00356245958586 33.0 31.0 34.0 30.0 34.0 2 32.192524198358875 34.0 31.0 34.0 30.0 34.0 3 32.267855155716624 34.0 31.0 34.0 30.0 34.0 4 35.7742326952285 37.0 35.0 37.0 35.0 37.0 5 35.81005228148794 37.0 35.0 37.0 35.0 37.0 6 35.83739805611112 37.0 36.0 37.0 35.0 37.0 7 35.79279437786032 37.0 36.0 37.0 35.0 37.0 8 35.783299498238065 37.0 36.0 37.0 35.0 37.0 9 37.46857496603959 39.0 38.0 39.0 35.0 39.0 10-11 37.39133721309092 39.0 37.0 39.0 35.0 39.0 12-13 37.31428091650501 39.0 37.0 39.0 34.5 39.0 14-15 38.57701444131992 40.0 38.0 41.0 34.0 41.0 16-17 38.51172699338837 40.0 38.0 41.0 34.0 41.0 18-19 38.49097755855978 40.0 38.0 41.0 34.0 41.0 20-21 38.40534845135515 40.0 38.0 41.0 34.0 41.0 22-23 38.25905502338381 40.0 38.0 41.0 34.0 41.0 24-25 38.23973393083605 40.0 38.0 41.0 34.0 41.0 26-27 38.250370682265896 40.0 38.0 41.0 34.0 41.0 28-29 38.16703985523591 40.0 38.0 41.0 34.0 41.0 30-31 38.018248144082364 40.0 38.0 41.0 33.5 41.0 32-33 37.83326466062147 40.0 38.0 41.0 33.0 41.0 34-35 37.66175255768257 40.0 37.0 41.0 33.0 41.0 36-37 37.47489912129004 40.0 37.0 41.0 33.0 41.0 38-39 37.267861922735676 40.0 37.0 41.0 31.5 41.0 40-41 37.07262790919162 40.0 36.5 41.0 31.0 41.0 42-43 36.83803240148975 40.0 36.0 41.0 31.0 41.0 44-45 36.62764302220083 39.5 36.0 41.0 30.0 41.0 46-47 36.479664857115644 40.0 36.0 41.0 30.0 41.0 48-49 36.26073825671564 40.0 36.0 41.0 29.5 41.0 50-51 35.654386782758635 39.0 35.5 40.5 28.5 41.0 52-53 35.7371401574961 39.0 35.5 40.0 28.5 41.0 54-55 35.9299059634982 39.0 36.0 41.0 28.0 41.0 56-57 35.79087630390432 39.0 36.0 41.0 28.0 41.0 58-59 35.60058146237788 39.0 35.0 41.0 27.5 41.0 60-61 35.283066913286916 39.0 35.0 40.5 26.5 41.0 62-63 34.90976004651699 38.5 35.0 40.0 26.0 41.0 64-65 34.51978375614671 38.0 34.0 40.0 25.0 41.0 66-67 34.13533386466965 38.0 34.0 40.0 23.5 41.0 68-69 33.69328085134112 37.0 34.0 39.5 22.5 41.0 70-71 33.22409935989012 36.5 33.0 39.0 21.5 41.0 72-73 32.712626756292075 36.0 32.5 39.0 19.5 40.0 74-75 32.17970821616365 35.0 32.0 38.0 18.0 40.0 76-77 31.595629508213158 35.0 31.5 37.0 14.0 39.0 78-79 31.011686391274054 35.0 31.0 37.0 8.0 39.0 80-81 30.462232765404995 34.5 30.5 36.0 6.5 38.5 82-83 29.913711735013557 34.0 30.0 36.0 2.0 37.0 84-85 29.389337183015286 34.0 29.0 35.0 2.0 37.0 86-87 28.847904730396948 34.0 29.0 35.0 2.0 36.5 88-89 28.281378917978717 34.0 28.5 35.0 2.0 36.0 90-91 27.65610961568344 33.5 26.5 35.0 2.0 35.5 92-93 27.069942154518614 33.0 26.0 35.0 2.0 35.0 94-95 26.496903712837785 33.0 25.0 35.0 2.0 35.0 96-97 25.668809305403087 32.0 22.0 34.5 2.0 35.0 98-99 24.625974576058788 32.0 10.0 34.0 2.0 35.0 100-101 21.75711664837065 28.0 2.0 33.0 2.0 34.5 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.3492905566938376 1101 2 0.2506219448207716 1101 3 0.31141879281651796 1101 4 0.1789332041299616 1101 5 0.2524002954842004 1101 6 0.10569045191119386 1101 7 0.23010733657532967 1101 8 0.19032795650930723 1101 9 0.20971624626066898 1101 10-11 0.24609721964106512 1101 12-13 0.11914839387274156 1101 14-15 0.20408314625743884 1101 16-17 0.27938192323880173 1101 18-19 0.2811131442599404 1101 20-21 0.3412085505858258 1101 22-23 0.32863518703205585 1101 24-25 0.38843485576061454 1101 26-27 0.2504186057849793 1101 28-29 0.3767064393648738 1101 30-31 0.4278851488405664 1101 32-33 0.3846489029406541 1101 34-35 0.49099464296418915 1101 36-37 0.4871953640298372 1101 38-39 0.4161221644125632 1101 40-41 0.5205686626166113 1101 42-43 0.5356624231176923 1101 44-45 0.6570652526288256 1101 46-47 0.799453689586386 1101 48-49 0.8073433689889598 1101 50-51 0.7970308702221445 1101 52-53 0.7203113208386043 1101 54-55 0.7738516773460375 1101 56-57 0.8523680050994713 1101 58-59 0.6625946266684437 1101 60-61 0.6824873802642628 1101 62-63 0.7165219180453661 1101 64-65 0.6327293232728834 1101 66-67 0.4884998964287348 1101 68-69 0.6051431163569632 1101 70-71 0.7155066624404114 1101 72-73 0.7431323581919784 1101 74-75 0.6097143872869424 1101 76-77 0.5928494952079753 1101 78-79 0.6738336831459897 1101 80-81 0.6714095729371827 1101 82-83 0.39493538761324487 1101 84-85 0.5539685461347581 1101 86-87 0.6776852079017459 1101 88-89 0.8305619539331204 1101 90-91 0.9112567211907425 1101 92-93 0.8866298894295461 1101 94-95 0.7667934815861308 1101 96-97 0.7598720991277084 1101 98-99 1.016902588621658 1101 100-101 1.206423144655595 1102 1 0.30906765902413014 1102 2 0.13490627750935857 1102 3 0.1572855147470733 1102 4 0.021223892165330938 1102 5 -0.014139724779226981 1102 6 0.11150927847470626 1102 7 -0.055982283024924584 1102 8 -0.028461764408071133 1102 9 0.15813431755108098 1102 10-11 0.14966749688155545 1102 12-13 -0.007351790340202058 1102 14-15 0.007120357273372235 1102 16-17 -0.012230630873744985 1102 18-19 0.0077157969264263215 1102 20-21 0.09746718556785794 1102 22-23 0.07181815654481483 1102 24-25 0.055527514874555095 1102 26-27 0.026370756471173706 1102 28-29 0.12997161390664758 1102 30-31 0.1711982187493888 1102 32-33 0.14185393195418783 1102 34-35 0.06340529968334607 1102 36-37 0.008637751429667162 1102 38-39 0.07908499944986858 1102 40-41 0.05013138712614307 1102 42-43 0.06283843859157656 1102 44-45 -0.17987520053502948 1102 46-47 0.05970283760148476 1102 48-49 0.012040799218304699 1102 50-51 -0.08118249442738801 1102 52-53 -0.03441949798796884 1102 54-55 -0.04275512011182059 1102 56-57 0.008756740477565472 1102 58-59 -0.04973958167641257 1102 60-61 -0.06709583793598028 1102 62-63 -0.08648535836616844 1102 64-65 -0.03454985145310019 1102 66-67 -0.16450622865185238 1102 68-69 -0.1092162831088146 1102 70-71 -0.0544758373293206 1102 72-73 0.03542765155109606 1102 74-75 -0.06481126436637652 1102 76-77 -0.016426778164042588 1102 78-79 0.0712351292176514 1102 80-81 0.20122904424601273 1102 82-83 0.1988372481640397 1102 84-85 0.25593386804688834 1102 86-87 0.3181913329823409 1102 88-89 0.2298020755136676 1102 90-91 0.18682087335063713 1102 92-93 0.22704505936599162 1102 94-95 0.21618673018150858 1102 96-97 0.2868627503204877 1102 98-99 0.46560503865389435 1102 100-101 0.508661332000166 1103 1 0.3632786519319282 1103 2 0.3382639902753155 1103 3 0.2571303729030987 1103 4 0.18069862620788513 1103 5 0.12546577167467632 1103 6 0.21265067918392333 1103 7 0.3035921850601824 1103 8 0.20466101927986813 1103 9 0.2771946012390245 1103 10-11 0.3165280908531827 1103 12-13 0.16538757136192572 1103 14-15 0.2713213767769176 1103 16-17 0.2825610357929236 1103 18-19 0.298185655082456 1103 20-21 0.2370283557806303 1103 22-23 0.17214506257318618 1103 24-25 0.30825357870433834 1103 26-27 0.12276370859883201 1103 28-29 0.32322294369388516 1103 30-31 0.43034050598343043 1103 32-33 0.4300797741527731 1103 34-35 0.4643915585485985 1103 36-37 0.5925085404367607 1103 38-39 0.43966788952078417 1103 40-41 0.5524308108850065 1103 42-43 0.6374616655419274 1103 44-45 0.6313448792522038 1103 46-47 0.6823203021404964 1103 48-49 0.5801923949629852 1103 50-51 0.4780373637286459 1103 52-53 0.6410736314446623 1103 54-55 0.6062752433633563 1103 56-57 0.526143708562671 1103 58-59 0.6405640530753658 1103 60-61 0.6679108110001906 1103 62-63 0.7260423455345375 1103 64-65 0.6610673914547007 1103 66-67 0.740275464610562 1103 68-69 0.7182992310755836 1103 70-71 0.7438785509685459 1103 72-73 0.7995954263737914 1103 74-75 0.6690927693215656 1103 76-77 0.7629726012685758 1103 78-79 0.8560644731892815 1103 80-81 0.897522262331119 1103 82-83 0.9567217783058837 1103 84-85 0.9608374584724224 1103 86-87 0.7311179730532622 1103 88-89 0.915867013456932 1103 90-91 1.2313798272513488 1103 92-93 1.3081565398624448 1103 94-95 1.257448919897822 1103 96-97 1.2320311900367997 1103 98-99 1.3117652780155957 1103 100-101 1.1212499844824322 1104 1 0.4041581886437555 1104 2 0.39065752389298325 1104 3 0.3374829611285577 1104 4 0.2776920092987041 1104 5 0.30383328714549407 1104 6 0.3002654036361747 1104 7 0.2612941419091541 1104 8 0.34991654905073233 1104 9 0.3117885968904659 1104 10-11 0.360399740585585 1104 12-13 0.2647133365400478 1104 14-15 0.2765389873723407 1104 16-17 0.47000092958786865 1104 18-19 0.4864706943866892 1104 20-21 0.3756745549778202 1104 22-23 0.43951039854074025 1104 24-25 0.5278988998282728 1104 26-27 0.47810701956220925 1104 28-29 0.5247572199106401 1104 30-31 0.45822795580780706 1104 32-33 0.5857134242196764 1104 34-35 0.6636872706242016 1104 36-37 0.7518277461985932 1104 38-39 0.6736354739830759 1104 40-41 0.4667443196239276 1104 42-43 0.6653692378784442 1104 44-45 0.7413215946310245 1104 46-47 0.6094906588054911 1104 48-49 0.6767067577326813 1104 50-51 0.5823190161788858 1104 52-53 0.6574644628964137 1104 54-55 0.7556784932797243 1104 56-57 0.8278457594909199 1104 58-59 0.7700205361829049 1104 60-61 0.8101939582656215 1104 62-63 0.8092874795452403 1104 64-65 0.7203437388790235 1104 66-67 0.7723218718274865 1104 68-69 0.8362900635726547 1104 70-71 0.7977753930408014 1104 72-73 0.8135302660208978 1104 74-75 0.7083926888315233 1104 76-77 0.702244688992284 1104 78-79 0.9395313468303605 1104 80-81 0.9052716508158163 1104 82-83 0.8238764812244312 1104 84-85 0.9161619535802998 1104 86-87 0.7876163684789184 1104 88-89 0.7763230104882091 1104 90-91 0.8689334001732405 1104 92-93 0.8490714869689846 1104 94-95 0.8492241697305687 1104 96-97 0.748882879282494 1104 98-99 0.9341251782915627 1104 100-101 0.7565380761365574 1105 1 0.36731247134204636 1105 2 0.35761875423973066 1105 3 0.33482387677523917 1105 4 0.23589743367205074 1105 5 0.14737887515136094 1105 6 0.23747792625922415 1105 7 0.19667176983526957 1105 8 0.2821189509819604 1105 9 0.2956428628966279 1105 10-11 0.27418238418816543 1105 12-13 0.1550780063594459 1105 14-15 0.2523753240911404 1105 16-17 0.2587592356611026 1105 18-19 0.3178666157019947 1105 20-21 0.23491670229570616 1105 22-23 0.25392709523919166 1105 24-25 0.3619346834992001 1105 26-27 0.3031212376398642 1105 28-29 0.4658414249099039 1105 30-31 0.2893841602098277 1105 32-33 0.24561427839564942 1105 34-35 0.30643900252619005 1105 36-37 0.31774630098215795 1105 38-39 0.39287810743147844 1105 40-41 0.35722270926200395 1105 42-43 0.2990847706663331 1105 44-45 0.3301233796229397 1105 46-47 0.3729980806048232 1105 48-49 0.2832061740040146 1105 50-51 0.24785117160473646 1105 52-53 0.18116865842604568 1105 54-55 0.14854834745793255 1105 56-57 0.20183961192402222 1105 58-59 0.06596439703647405 1105 60-61 0.22021169458891876 1105 62-63 0.21582070172996026 1105 64-65 0.12903974583763045 1105 66-67 0.18960105881883038 1105 68-69 0.15203754257665025 1105 70-71 0.3815310936032503 1105 72-73 0.2892759493182737 1105 74-75 0.20623040010917748 1105 76-77 0.237724156302356 1105 78-79 0.21738867198644485 1105 80-81 0.22826520288969832 1105 82-83 0.2513246010526302 1105 84-85 0.12346760244177446 1105 86-87 0.2458623450667723 1105 88-89 0.4070881526479013 1105 90-91 0.21795787758912866 1105 92-93 0.17613018152499293 1105 94-95 0.40090554887293806 1105 96-97 0.2958709906629302 1105 98-99 0.4815992961816882 1105 100-101 0.1981180846636832 1106 1 -0.01380468140140323 1106 2 0.052399447473760574 1106 3 -0.07769047664294249 1106 4 0.0051940055853165745 1106 5 -0.038270447521171036 1106 6 0.053456045591183 1106 7 0.11944821618858725 1106 8 0.029038856504250532 1106 9 0.03859213690698482 1106 10-11 -0.4584850819743096 1106 12-13 -0.2172242963423585 1106 14-15 -0.07698946142022578 1106 16-17 -0.10595631180760279 1106 18-19 -0.06490610886047676 1106 20-21 -0.10821726678747012 1106 22-23 -0.1536382140156647 1106 24-25 -0.0829307598170601 1106 26-27 -0.09423835852178541 1106 28-29 -0.24128945902985066 1106 30-31 -0.10869767495042737 1106 32-33 -0.10122393045163847 1106 34-35 -0.41556750435672285 1106 36-37 -0.4361869443232891 1106 38-39 -0.2214479502976232 1106 40-41 -0.0925118366506652 1106 42-43 -0.12844705689645508 1106 44-45 -0.05945339409409911 1106 46-47 0.017228295006908922 1106 48-49 -0.0706222937529688 1106 50-51 0.04045630017481017 1106 52-53 0.016974450186552303 1106 54-55 -0.10038716746673515 1106 56-57 -0.15452871361631537 1106 58-59 -0.09736355717107159 1106 60-61 -0.32451987966128826 1106 62-63 -0.3086477890309638 1106 64-65 -0.3050875615284383 1106 66-67 -0.5158701660638911 1106 68-69 -0.6531116800787657 1106 70-71 -0.3969379616773665 1106 72-73 -0.09278763944980994 1106 74-75 -0.08736900921280721 1106 76-77 0.08744771475755542 1106 78-79 0.11570900950575336 1106 80-81 -0.011764581054091394 1106 82-83 -0.0790769003581886 1106 84-85 0.01737624738292709 1106 86-87 0.11833931708698131 1106 88-89 -0.13893498881284927 1106 90-91 -0.4064018685981985 1106 92-93 -0.055673915714727684 1106 94-95 -0.007362404680648638 1106 96-97 -0.11533424446384899 1106 98-99 -0.1991023046692284 1106 100-101 -0.328668951963742 1107 1 0.2323857947890744 1107 2 0.14940982360865007 1107 3 0.14198156337928935 1107 4 0.07363017382693471 1107 5 0.16692410500800747 1107 6 0.13010603632677942 1107 7 0.1278778993458971 1107 8 0.18666994785129987 1107 9 0.2696946012390242 1107 10-11 0.10433315037700197 1107 12-13 0.018304238028584052 1107 14-15 0.14376929344359013 1107 16-17 0.1236027024595856 1107 18-19 0.18288803603483217 1107 20-21 0.13271287959015154 1107 22-23 0.22977155066842414 1107 24-25 0.26733095965672504 1107 26-27 0.2625181728845476 1107 28-29 0.31210687226531064 1107 30-31 0.29038514884057065 1107 32-33 0.3059800717718275 1107 34-35 0.2551504871200265 1107 36-37 0.31384038567485817 1107 38-39 0.26718277047316263 1107 40-41 0.2853846799326263 1107 42-43 0.4372533322085985 1107 44-45 0.3757124387760129 1107 46-47 0.5267697069024067 1107 48-49 0.4959364425820283 1107 50-51 0.49470403039531163 1107 52-53 0.48353642906370453 1107 54-55 0.6189166124109775 1107 56-57 0.5660693038007665 1107 58-59 0.54626345783727 1107 60-61 0.5916756919525668 1107 62-63 0.6412730002964437 1107 64-65 0.3986492366927905 1107 66-67 0.5220388574676988 1107 68-69 0.5293855405993853 1107 70-71 0.6597490866828366 1107 72-73 0.5420284620880764 1107 74-75 0.4730585431310885 1107 76-77 0.6187910536495345 1107 78-79 0.6070912589035657 1107 80-81 0.5660416075692147 1107 82-83 0.6424137425916001 1107 84-85 0.6338062084724214 1107 86-87 0.6312891040056421 1107 88-89 0.6794905253616932 1107 90-91 0.7114839939180122 1107 92-93 0.8025389803386354 1107 94-95 0.8280813603740107 1107 96-97 0.8341145233701361 1107 98-99 0.6528441470632167 1107 100-101 0.6187499844824345 1108 1 0.2283865728033092 1108 2 0.1857929727356762 1108 3 0.19480506856223911 1108 4 0.15825523961490262 1108 5 0.1974959454693277 1108 6 0.2124281799848191 1108 7 0.20118949404624686 1108 8 0.21191239081598923 1108 9 0.22845801626385054 1108 10-11 0.2718724515371491 1108 12-13 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58-59 -0.8854646326118498 2316 60-61 -0.7556278039976192 2316 62-63 -0.7222893708697811 2316 64-65 -0.5583349596967366 2316 66-67 -0.759498506931493 2316 68-69 -0.653207063560771 2316 70-71 -0.7753377394774503 2316 72-73 -0.5973361661418721 2316 74-75 -0.603180655652924 2316 76-77 -0.6543820573088865 2316 78-79 -0.5899690405113454 2316 80-81 -0.5751934268905678 2316 82-83 -0.6423981340611178 2316 84-85 -0.7917010062193164 2316 86-87 -0.9686102234559932 2316 88-89 -1.1346646373592755 2316 90-91 -1.2105375772815208 2316 92-93 -1.160867540993113 2316 94-95 -1.3066551420100652 2316 96-97 -1.4551144519978898 2316 98-99 -1.3147189945029858 2316 100-101 -1.3428059111596014 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 5350.0 3 1189.0 4 1031.0 5 1213.0 6 1248.0 7 1579.0 8 1910.0 9 2367.0 10 3137.0 11 4042.0 12 5520.0 13 7319.0 14 8682.0 15 11045.0 16 14561.0 17 20323.0 18 18172.0 19 13647.0 20 12429.0 21 12358.0 22 13195.0 23 14644.0 24 16311.0 25 18723.0 26 21586.0 27 24938.0 28 29318.0 29 35529.0 30 42590.0 31 52401.0 32 66079.0 33 85982.0 34 117731.0 35 173233.0 36 260279.0 37 420956.0 38 403915.0 39 50431.0 40 7.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.050233895562585 19.438217416579935 14.781262562503967 22.730286125353512 2 14.329410643670496 21.05965280058978 42.05832127605688 22.55261527968284 3 19.048032231805788 25.58211501915682 30.219223826579 25.15062892245839 4 11.614956524356142 17.808837581414945 39.580165977393165 30.996039916835745 5 12.754229996844465 38.55466474826208 35.085181879705395 13.605923375188064 6 29.594016021774515 37.6027956812265 19.066553993701287 13.736634303297693 7 26.876167525778367 33.813803841623816 21.559974959017463 17.75005367358036 8 24.264520390410166 35.993275500362856 20.933616128768552 18.808587980458427 9 25.6872939697875 17.416746617129977 21.420722658571485 35.47523675451104 10-11 23.55615385039853 27.348938494855386 28.64787204851104 20.44703560623504 12-13 25.38472925809114 25.098669436687217 28.244198387667385 21.272402917554253 14-15 22.08394877208997 26.721268309564234 27.673202647159872 23.52158027118593 16-17 20.404007371123214 29.678275751030984 28.74484758688004 21.17286929096576 18-19 19.933041088195168 28.999854546184096 30.88631530374769 20.180789061873046 20-21 21.807430631760464 27.830945289230286 30.320739927888386 20.040884151120867 22-23 20.609779372614778 28.368567187259785 29.748795616895695 21.272857823229742 24-25 20.388865600869778 28.021758451983075 30.838722398366613 20.75065354878054 26-27 20.88326600959771 28.083252365944837 30.460056952218622 20.573424672238826 28-29 20.173907155047985 28.332319669140006 30.801630724433405 20.692142451378604 30-31 20.633707966621603 28.66988043671824 30.66455815260057 20.031853444059582 32-33 20.201086621195984 28.497320567298328 30.413805938142552 20.887786873363133 34-35 20.68039222817356 28.42252189321953 30.401997509383705 20.495088369223204 36-37 20.793937907341057 28.300876468283764 30.331681521933778 20.5735041024414 38-39 20.903223282937518 28.179517357546864 30.572019013643427 20.345240345872195 40-41 21.063515131820452 27.976090179110525 30.737143418806507 20.22325127026252 42-43 20.33323270475575 28.392503605578767 30.594815187760894 20.679448501904588 44-45 20.758796514484544 28.75871128179931 29.94901079948259 20.53348140423356 46-47 20.50152151709469 28.44434717164287 29.369165825557776 21.68496548570466 48-49 20.81082131320361 28.298069968499256 29.793133013855062 21.097975704442067 50-51 21.41659720350721 28.72465639238642 29.253520685397692 20.605225718708674 52-53 20.810847195206545 28.71064210307553 29.795241290979884 20.683269410738042 54-55 20.691598480094804 28.48400812949227 29.920532633664195 20.903860756748735 56-57 20.97587243204 29.087753110832786 29.02618323955794 20.910191217569274 58-59 20.78736213654636 28.837565039863712 29.229483802328954 21.14558902126098 60-61 21.000080725668607 28.292416450186497 29.398107409262593 21.309395414882303 62-63 21.495055104277487 28.087539143539846 29.451383798710708 20.966021953471955 64-65 21.249559107872486 28.160992872776646 29.594108502957205 20.995339516393667 66-67 20.66104784935226 28.418008724766775 29.780968397473963 21.139975028407004 68-69 20.84964171281978 28.700324722041042 29.45427445404634 20.99575911109284 70-71 20.79124085248588 28.266765156176582 29.195169199862995 21.746824791474538 72-73 20.66725570881126 28.092019949874587 29.193264660780926 22.047459680533226 74-75 21.017918433146217 28.031504551688176 29.09334784123872 21.857229173926886 76-77 21.01846176600724 27.7890377545559 29.44592140243742 21.74657907699944 78-79 20.710862493673783 27.760405138580186 29.622738134598837 21.905994233147197 80-81 20.601131633910512 28.485369275026347 29.18073354663854 21.732765544424602 82-83 20.7877108444608 28.322058380407704 29.10487084985553 21.785359925275966 84-85 21.034513012568873 27.836043136252197 29.04119100977133 22.088252841407595 86-87 20.910570898978214 27.863098542830894 29.311494804597473 21.91483575359342 88-89 21.581449461416565 27.295022365755322 29.368994844843844 21.754533327984273 90-91 21.055859859977335 27.655893011229548 29.531847645671792 21.75639948312132 92-93 20.738631377793425 27.904389697951594 29.482952018344438 21.874026905910547 94-95 20.71475626401641 28.23794548135948 28.986766178411465 22.06053207621265 96-97 20.76663537378941 27.721101228446027 28.88445405964904 22.627809338115526 98-99 21.153546258433472 27.381693188285528 28.937413659532112 22.527346893748888 100-101 21.703231799551855 27.085631076492405 29.081049189382576 22.13008793457316 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 2446.0 1 1418.5 2 563.5 3 774.0 4 595.5 5 539.0 6 1250.5 7 2589.0 8 3617.5 9 3628.5 10 2799.5 11 2090.5 12 2017.0 13 2079.5 14 2089.0 15 2061.5 16 2137.0 17 2340.5 18 2685.5 19 3122.0 20 3693.0 21 4515.0 22 5469.0 23 6677.0 24 8416.0 25 10535.5 26 13082.0 27 16181.0 28 19714.5 29 23830.5 30 28493.5 31 33649.0 32 38695.0 33 44152.5 34 50340.0 35 56167.0 36 61312.5 37 65372.5 38 68403.5 39 70452.0 40 71869.5 41 73151.0 42 74248.5 43 75351.5 44 77297.5 45 79856.0 46 81476.5 47 83291.5 48 100521.0 49 99569.0 50 78530.0 51 70162.0 52 62256.0 53 55366.0 54 49070.0 55 42139.0 56 35372.5 57 29215.0 58 23118.0 59 17799.0 60 13309.5 61 9667.0 62 6990.5 63 4858.0 64 3264.5 65 2202.5 66 1577.0 67 1195.5 68 890.0 69 677.5 70 472.5 71 331.5 72 266.5 73 209.0 74 163.0 75 129.0 76 93.5 77 58.5 78 41.5 79 37.0 80 28.0 81 21.5 82 15.5 83 10.0 84 7.0 85 5.0 86 3.0 87 3.5 88 3.0 89 1.0 90 1.5 91 1.5 92 1.0 93 1.0 94 0.0 95 0.5 96 0.5 97 0.0 98 0.0 99 0.0 100 0.5 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.4960475596124252 2 0.0508779580645323 3 0.05829661598921287 4 0.06641703885271458 5 0.08255763244560069 6 0.10937507832197978 7 0.07208128443034231 8 0.05348451355158222 9 0.05147947086923613 10-11 0.07654250439856239 12-13 0.11052797786432879 14-15 0.0568930861115706 16-17 0.06235682742096372 18-19 0.06065254114096954 20-21 0.07681819776738497 22-23 0.06589071514859873 24-25 0.09090362261086632 26-27 0.06862258580329529 28-29 0.06904865737329384 30-31 0.07343468824092593 32-33 0.07273292330210479 34-35 0.0745625247497456 36-37 0.07022661994917216 38-39 0.04125375318927101 40-41 0.044812703950435344 42-43 0.0420056441951508 44-45 0.021554208835220582 46-47 0.029022992826959804 48-49 0.02869717339107856 50-51 0.02543897903226615 52-53 0.025088096562855582 54-55 0.02523847476403154 56-57 0.024461520724622425 58-59 0.021253452432868664 60-61 0.02812072361990406 62-63 0.019448914018757174 64-65 0.021779776136984518 66-67 0.015213261352301036 68-69 0.017092988867000506 70-71 0.04255703093279598 72-73 0.03869732376927974 74-75 0.032832573923417395 76-77 0.022180784673453736 78-79 0.014762126748773164 80-81 0.016641854263472634 82-83 0.021604334902279233 84-85 0.018045384141114906 86-87 0.008897376902910821 88-89 0.04260715699985464 90-91 0.054060963322756735 92-93 0.03443660806929427 94-95 0.05521386286510574 96-97 0.045414216755139174 98-99 0.03433635593517697 100-101 0.17205772517882473 >>END_MODULE >>Sequence Length Distribution pass #Length Count 101 1994970.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 67.15368332321701 #Duplication Level Percentage of deduplicated Percentage of total 1 77.46637619210036 52.02152495001504 2 14.207813924844451 19.082140740483943 3 4.497465361198341 9.060640938691535 4 1.8341304814501358 4.926744700990478 5 0.8270041794419135 2.7768188386609594 6 0.4287520176853071 1.727536633189767 7 0.24835696462520676 1.167465946748958 8 0.1454929604509633 0.7816310553505059 9 0.09198563824061072 0.5559456978624575 >10 0.23433978991201806 2.3126530867603785 >50 0.008576978802633575 0.4076280292609623 >100 0.007168279649889125 0.9900670676040519 >500 9.701179640128922E-4 0.4517693506088363 >1k 0.0011193668815532823 1.4323318446035813 >5k 3.731222938510941E-4 1.7597706617836175 >10k+ 7.46244587702188E-5 0.5453304573849336 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 10882 0.5454718617322566 No Hit GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 8792 0.4407083815796729 No Hit GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA 8495 0.425820939663253 No Hit GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA 6500 0.32581943588124135 No Hit TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 6026 0.30205968009544004 No Hit GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT 5303 0.2658185336120342 No Hit CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG 2918 0.14626786367714803 No Hit GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT 2776 0.13914996215481937 No Hit CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA 2737 0.1371950455395319 No Hit AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA 2727 0.1366937848689454 No Hit TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC 2256 0.11308440728432007 No Hit GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG 2137 0.10711940530434043 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10-11 0.0 0.0 0.0 0.0 0.0 12-13 0.0 0.0 0.0 0.0 0.0 14-15 0.0 0.0 0.0 0.0 0.0 16-17 0.0 0.0 0.0 2.5063033529326255E-5 0.0 18-19 0.0 0.0 0.0 1.0025213411730502E-4 0.0 20-21 0.0 0.0 0.0 2.756933688225888E-4 0.0 22-23 0.0 0.0 0.0 6.265758382331564E-4 0.0 24-25 0.0 0.0 0.0 0.001152899542349008 0.0 26-27 0.0 0.0 0.0 0.002481240319403299 0.0 28-29 0.0 0.0 0.0 0.005814623778803691 0.0 30-31 0.0 0.0 0.0 0.017995258074056254 0.0 32-33 0.0 0.0 0.0 0.04280766126808924 0.0 34-35 0.0 0.0 0.0 0.08401128839030161 0.0 36-37 0.0 0.0 0.0 0.14879923006360996 0.0 38-39 0.0 0.0 0.0 0.23682060381860381 0.0 40-41 0.0 0.0 0.0 0.3279999197982927 0.0 42-43 0.0 0.0 0.0 0.4168734366932836 0.0 44-45 0.0 0.0 0.0 0.5279778643287869 0.0 46-47 0.0 0.0 0.0 0.6569522348706998 0.0 48-49 0.0 0.0 0.0 0.7979568615066893 0.0 50-51 0.0 0.0 0.0 0.9357032937838664 0.0 52-53 0.0 0.0 0.0 1.068963443059294 0.0 54-55 0.0 0.0 0.0 1.2155320631387942 0.0 56-57 0.0 0.0 0.0 1.3753339649217784 0.0 58-59 0.0 0.0 0.0 1.558995874624681 0.0 60-61 0.0 0.0 0.0 1.7630089675533969 0.0 62-63 0.0 0.0 0.0 1.9566710276345007 0.0 64-65 0.0 0.0 0.0 2.1480774146979655 0.0 66-67 0.0 0.0 0.0 2.357027925231958 0.0 68-69 0.0 0.0 0.0 2.5887106071770503 0.0 70-71 0.0 0.0 0.0 2.8415464894208933 0.0 72-73 0.0 0.0 0.0 3.098542835230605 0.0 74-75 0.0 0.0 0.0 3.3531581928550303 0.0 76-77 0.0 0.0 0.0 3.6188764743329473 0.0 78-79 0.0 0.0 0.0 3.9077028727249035 0.0 80-81 0.0 0.0 0.0 4.20951192248505 0.0 82-83 0.0 0.0 0.0 4.516183200749886 0.0 84-85 0.0 0.0 0.0 4.860148272906359 0.0 86-87 0.0 0.0 0.0 5.223512133014532 0.0 88-89 0.0 0.0 0.0 5.588003829631523 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGACCG 105 1.8954561E-6 31.681934 5 GTATAGC 1585 0.0 23.496351 1 TAGCCCT 2390 0.0 23.46319 4 GTACATG 8575 0.0 22.82889 1 TACATGG 8735 0.0 22.306114 2 GAGTACT 7195 0.0 22.202238 12-13 ATAGCCC 1405 0.0 21.985683 3 GTGTAGC 1760 0.0 21.431349 1 ACATGGG 8815 0.0 21.348915 3 GTAGCCC 1410 0.0 21.233637 3 AGTACTT 7325 0.0 20.867077 12-13 TCGCCGC 70 4.8253778E-4 20.375395 94-95 GTACTTT 7920 0.0 20.186926 14-15 TATAGCC 1770 0.0 19.599842 2 GTCCTAC 810 0.0 19.451988 1 CGCCGTA 150 3.6834535E-9 19.017036 94-95 TAGGACG 770 0.0 18.515415 4 CATGGGG 3210 0.0 18.505802 4 ACCGTCT 165 0.0014539678 17.291039 8 TACTTTT 8950 0.0 17.120523 14-15 >>END_MODULE