FastQCFastQC Report
Thu 26 May 2016
SRR938113_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR938113_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1994970
Sequences flagged as poor quality0
Sequence length101
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT103430.518453911587643No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT83380.4179511471350446No Hit
GTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGA81690.40947984180213237No Hit
GAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTGGTATCAACGCA66360.33263658100121807No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT60270.30210980616249866No Hit
GCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACT53310.26722206348967653No Hit
CTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTG32040.16060391885592265No Hit
GCGTTGATACCACTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCT28010.14040311383128568No Hit
CTGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCA27680.13874895361835016No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA26960.13513987679012715No Hit
TGCTTGTACTCTGCGTTGATACCACTGCTTGTACTCTGCGTTGATACCAC24530.12295924249487461No Hit
GTATCAACGCAGAGTACAAGCAGTGGTATCAACGCAGAGTACAAGCAGTG20360.10205667253141651No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20190.10120452939141943No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAGC13500.028.1497151
TTCGGCG350.00839481227.14232818-19
GATCGTA700.00839690327.1402876
TAGCCCT24750.025.9066374
ACCGTGC1858.985808E-925.6732448
GTGTAGC17600.025.6406331
GTAGCCC15000.022.482333
ATAGCCC15200.021.8740253
TGTAGCC19000.021.2511832
GTACATG93100.021.2256721
TATAGCC16450.021.08022
GAGTACT72450.021.07633412-13
TACATGG93350.021.0161692
AGTACTT74450.019.96788812-13
ACATGGG94700.019.962153
TAGGACG7800.019.4853344
GTACTTT79550.019.31463814-15
CATGGGG34550.018.6957684
AGGACGT8600.018.2250185
CCCTGGC32100.017.7553277
Produced by FastQC (version 0.11.5)